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Characterizing the genetic variability of <jats:italic>H. contortus<\/jats:italic> populations is crucial for understanding patterns of disease transmission and developing effective control strategies against haemonchosis. This study aimed to identify the genetic variability of <jats:italic>H. contortus<\/jats:italic> isolates in small ruminants from slaughterhouses in Bangladesh. During January to December 2015, 400 abomasa samples were collected and 186 were found to be positive for <jats:italic>Haemonchus<\/jats:italic>. A 321-bp fragment of the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA and an 800-bp fragment of the mitochondrial nicotinamide dehydrogenase subunit-4 gene (<jats:italic>nad<\/jats:italic>4) were amplified using polymerase chain reaction (PCR) and directly sequenced. The results showed 10 genotypes (ITS-2) and 45 haplotypes (<jats:italic>nad<\/jats:italic>4) among the 186 worms. The sequences were 98.5 to 100% identical to reference sequences from the GenBank database. ITS-2 sequence analysis revealed four nucleotide substitutions at positions 30, 41, 42, and 216. There was one transition (C\/T) at position 42 and three transversions (C\/A at position 30, G\/C at position 41, and T\/A at position 216). The <jats:italic>nad<\/jats:italic>4 gene sequences showed 15 substitutions, all of which were transitions. The pairwise distance of ITS-2 between <jats:italic>H. contortus<\/jats:italic> populations ranged from 0.005 to 1.477. The nucleotide diversity (<jats:italic>\u03bc<\/jats:italic>) among the populations was 0.009524 using ITS-2 and 0.00394 using <jats:italic>nad<\/jats:italic>4. This study indicated low genetic deviation among <jats:italic>H. contortus<\/jats:italic> populations in Bangladesh.<\/jats:p>","DOI":"10.1007\/s00436-023-08000-4","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T06:01:48Z","timestamp":1697695308000},"page":"3101-3107","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genetic variability of Haemonchus contortus isolates in small ruminants from slaughterhouses in Bangladesh"],"prefix":"10.1007","volume":"122","author":[{"given":"Muhammad Abdul","family":"Mannan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharmin","family":"Chowdhury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Alamgir","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Hazzaz Bin","family":"Kabir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,19]]},"reference":[{"key":"8000_CR1","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.vetpar.2012.12.014","volume":"193","author":"H Akkari","year":"2013","unstructured":"Akkari H, Jebali J, Gharbi M, Mhadhbi M, Awadi S, Darghouth MA (2013) Epidemiological study of sympatric Haemonchus species and genetic characterization of Haemonchus contortus in domestic ruminants in Tunisia. 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