{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T14:31:31Z","timestamp":1759847491735},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Major Industrial Research Project for the Transformation of New and Old Kinetic Energy in Shandong Province","award":["202123-50"],"award-info":[{"award-number":["202123-50"]}]},{"name":"National Key Research and Development Program of China","award":["2017YFD0500400","2017YFD0500400"],"award-info":[{"award-number":["2017YFD0500400","2017YFD0500400"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2024,1]]},"DOI":"10.1007\/s00436-023-08079-9","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T09:02:15Z","timestamp":1702544535000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A candidate virulence factor of Eimeria tenella (EtROP30) predicted by virulence enhancement of transgenic Toxoplasma gondii"],"prefix":"10.1007","volume":"123","author":[{"given":"Bingxiang","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningning","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junpeng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"8079_CR1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-022-07499-3","author":"W Bingxiang","year":"2022","unstructured":"Bingxiang W et al (2022) Characterization of the Eimeria tenella rhoptry protein with a nuclear localization sequence (EtROP30). Parasitol Res. https:\/\/doi.org\/10.1007\/s00436-022-07499-3","journal-title":"Parasitol Res"},{"issue":"10","key":"8079_CR2","doi-asserted-by":"publisher","first-page":"e3000475","DOI":"10.1371\/journal.pbio.3000475","volume":"17","author":"CP Choi","year":"2019","unstructured":"Choi CP et al (2019) A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex. PLoS Biol 17(10):e3000475. https:\/\/doi.org\/10.1371\/journal.pbio.3000475","journal-title":"PLoS Biol"},{"issue":"2","key":"8079_CR3","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1128\/IAI.05974-11","volume":"80","author":"EY Denkers","year":"2012","unstructured":"Denkers EY, Bzik DJ, Fox BA, Butcher BA (2012) An inside job: hacking into Janus kinase\/signal transducer and activator of transcription signaling cascades by the intracellular protozoan Toxoplasma gondii. Infect Immun 80(2):476\u2013482. https:\/\/doi.org\/10.1128\/IAI.05974-11","journal-title":"Infect Immun"},{"issue":"7","key":"8079_CR4","doi-asserted-by":"publisher","first-page":"e13027","DOI":"10.1111\/cmi.13027","volume":"21","author":"MA Diallo","year":"2019","unstructured":"Diallo MA et al (2019) Eimeria tenella ROP kinase EtROP1 induces G0\/G1 cell cycle arrest and inhibits host cell apoptosis. Cell Microbiol 21(7):e13027. https:\/\/doi.org\/10.1111\/cmi.13027","journal-title":"Cell Microbiol"},{"issue":"15","key":"8079_CR5","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1016\/j.micinf.2012.09.005","volume":"14","author":"JF Dubremetz","year":"2012","unstructured":"Dubremetz JF, Lebrun M (2012) Virulence factors of Toxoplasma gondii. Microbes Infect 14(15):1403\u20131410. https:\/\/doi.org\/10.1016\/j.micinf.2012.09.005","journal-title":"Microbes Infect"},{"issue":"5","key":"8079_CR6","doi-asserted-by":"publisher","first-page":"4099","DOI":"10.3892\/mmr.2016.5758","volume":"14","author":"W Fan","year":"2016","unstructured":"Fan W et al (2016) Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16. Mol Med Rep 14(5):4099\u20134108. https:\/\/doi.org\/10.3892\/mmr.2016.5758","journal-title":"Mol Med Rep"},{"issue":"6","key":"8079_CR7","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1016\/j.chom.2010.11.005","volume":"8","author":"SJ Fentress","year":"2010","unstructured":"Fentress SJ et al (2010) Phosphorylation of immunity-related GTPases by a Toxoplasma gondii-secreted kinase promotes macrophage survival and virulence. Cell Host Microbe 8(6):484\u2013495. https:\/\/doi.org\/10.1016\/j.chom.2010.11.005","journal-title":"Cell Host Microbe"},{"key":"8079_CR8","doi-asserted-by":"publisher","first-page":"915","DOI":"10.3389\/fimmu.2019.00915","volume":"10","author":"M Garcia-Sanchez","year":"2019","unstructured":"Garcia-Sanchez M et al (2019) Differential responses of bovine monocyte-derived macrophages to infection by Neospora caninum isolates of high and low virulence. Front Immunol 10:915. https:\/\/doi.org\/10.3389\/fimmu.2019.00915","journal-title":"Front Immunol"},{"key":"8079_CR9","doi-asserted-by":"publisher","unstructured":"Goswami D et al (2020) A replication-competent late liver stage-attenuated human malaria parasite. JCI Insight 5(13). https:\/\/doi.org\/10.1172\/jci.insight.135589","DOI":"10.1172\/jci.insight.135589"},{"issue":"6","key":"8079_CR10","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1093\/abbs\/gmz040","volume":"51","author":"P Hao","year":"2019","unstructured":"Hao P, Cui X, Liu J, Li M, Fu Y, Liu Q (2019) Identification and characterization of stearoyl-CoA desaturase in Toxoplasma gondii. Acta Biochim Biophys Sin (shanghai) 51(6):615\u2013626. https:\/\/doi.org\/10.1093\/abbs\/gmz040","journal-title":"Acta Biochim Biophys Sin (shanghai)"},{"issue":"9","key":"8079_CR11","doi-asserted-by":"publisher","first-page":"1126","DOI":"10.7150\/ijbs.21247","volume":"13","author":"C He","year":"2017","unstructured":"He C et al (2017) Host cell vimentin restrains Toxoplasma gondii invasion and phosphorylation of vimentin is partially regulated by interaction with TgROP18. Int J Biol Sci 13(9):1126\u20131137. https:\/\/doi.org\/10.7150\/ijbs.21247","journal-title":"Int J Biol Sci"},{"issue":"6","key":"8079_CR12","doi-asserted-by":"publisher","first-page":"e99744","DOI":"10.1371\/journal.pone.0099744","volume":"9","author":"T Lei","year":"2014","unstructured":"Lei T, Wang H, Liu J, Nan H, Liu Q (2014) ROP18 is a key factor responsible for virulence difference between Toxoplasma gondii and Neospora caninum. PLoS ONE 9(6):e99744. https:\/\/doi.org\/10.1371\/journal.pone.0099744","journal-title":"PLoS ONE"},{"issue":"11","key":"8079_CR13","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/j.pt.2011.08.001","volume":"27","author":"MB Melo","year":"2011","unstructured":"Melo MB, Jensen KD, Saeij JP (2011) Toxoplasma gondii effectors are master regulators of the inflammatory response. Trends Parasitol 27(11):487\u2013495. https:\/\/doi.org\/10.1016\/j.pt.2011.08.001","journal-title":"Trends Parasitol"},{"issue":"37","key":"8079_CR14","doi-asserted-by":"publisher","first-page":"28731","DOI":"10.1074\/jbc.M110.112359","volume":"285","author":"YC Ong","year":"2010","unstructured":"Ong YC, Reese ML, Boothroyd JC (2010) Toxoplasma rhoptry protein 16 (ROP16) subverts host function by direct tyrosine phosphorylation of STAT6. J Biol Chem 285(37):28731\u201328740. https:\/\/doi.org\/10.1074\/jbc.M110.112359","journal-title":"J Biol Chem"},{"issue":"2","key":"8079_CR15","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.chom.2010.07.004","volume":"8","author":"L Peixoto","year":"2010","unstructured":"Peixoto L et al (2010) Integrative genomic approaches highlight a family of parasite-specific kinases that regulate host responses. Cell Host Microbe 8(2):208\u2013218. https:\/\/doi.org\/10.1016\/j.chom.2010.07.004","journal-title":"Cell Host Microbe"},{"issue":"12","key":"8079_CR16","doi-asserted-by":"publisher","first-page":"e0004268","DOI":"10.1371\/journal.pntd.0004268","volume":"9","author":"SL Ranasinghe","year":"2015","unstructured":"Ranasinghe SL, Fischer K, Zhang W, Gobert GN, McManus DP (2015) Cloning and characterization of two potent kunitz type protease inhibitors from Echinococcus granulosus. PLoS Negl Trop Dis 9(12):e0004268. https:\/\/doi.org\/10.1371\/journal.pntd.0004268","journal-title":"PLoS Negl Trop Dis"},{"issue":"23","key":"8079_CR17","doi-asserted-by":"publisher","first-page":"9625","DOI":"10.1073\/pnas.1015980108","volume":"108","author":"ML Reese","year":"2011","unstructured":"Reese ML, Zeiner GM, Saeij JP, Boothroyd JC, Boyle JP (2011) Polymorphic family of injected pseudokinases is paramount in Toxoplasma virulence. Proc Natl Acad Sci U S A 108(23):9625\u20139630. https:\/\/doi.org\/10.1073\/pnas.1015980108","journal-title":"Proc Natl Acad Sci U S A"},{"key":"8079_CR18","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/j.csbj.2020.12.045","volume":"19","author":"B Ren","year":"2021","unstructured":"Ren B et al (2021) Toxoplasma and Eimeria co-opt the host cFos expression for intracellular development in mammalian cells. Comput Struct Biotechnol J 19:719\u2013731. https:\/\/doi.org\/10.1016\/j.csbj.2020.12.045","journal-title":"Comput Struct Biotechnol J"},{"issue":"5806","key":"8079_CR19","doi-asserted-by":"publisher","first-page":"1780","DOI":"10.1126\/science.1133690","volume":"314","author":"JP Saeij","year":"2006","unstructured":"Saeij JP et al (2006) Polymorphic secreted kinases are key virulence factors in toxoplasmosis. Science 314(5806):1780\u20131783. https:\/\/doi.org\/10.1126\/science.1133690","journal-title":"Science"},{"key":"8079_CR20","doi-asserted-by":"publisher","unstructured":"Shen B, Brown KM, Lee TD, Sibley LD (2014) Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR\/CAS9. mBio 5(3):e01114\u201314. https:\/\/doi.org\/10.1128\/mBio.01114-14","DOI":"10.1128\/mBio.01114-14"},{"issue":"12","key":"8079_CR21","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1016\/j.ijpara.2017.08.002","volume":"47","author":"CE Suarez","year":"2017","unstructured":"Suarez CE, Bishop RP, Alzan HF, Poole WA, Cooke BM (2017) Advances in the application of genetic manipulation methods to apicomplexan parasites. Int J Parasitol 47(12):701\u2013710. https:\/\/doi.org\/10.1016\/j.ijpara.2017.08.002","journal-title":"Int J Parasitol"},{"key":"8079_CR22","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1186\/1471-2148-13-117","volume":"13","author":"E Talevich","year":"2013","unstructured":"Talevich E, Kannan N (2013) Structural and evolutionary adaptation of rhoptry kinases and pseudokinases, a family of coccidian virulence factors. BMC Evol Biol 13:117. https:\/\/doi.org\/10.1186\/1471-2148-13-117","journal-title":"BMC Evol Biol"},{"issue":"5806","key":"8079_CR23","doi-asserted-by":"publisher","first-page":"1776","DOI":"10.1126\/science.1133643","volume":"314","author":"S Taylor","year":"2006","unstructured":"Taylor S et al (2006) A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii. Science 314(5806):1776\u20131780. https:\/\/doi.org\/10.1126\/science.1133643","journal-title":"Science"},{"key":"8079_CR24","doi-asserted-by":"publisher","first-page":"790","DOI":"10.3389\/fphar.2019.00790","volume":"10","author":"DR Vandana","year":"2019","unstructured":"Vandana DR, Tiwari R, Katyal A, Pandey KC (2019) Metacaspases: potential drug target against protozoan parasites. Front Pharmacol 10:790. https:\/\/doi.org\/10.3389\/fphar.2019.00790","journal-title":"Front Pharmacol"},{"issue":"7","key":"8079_CR25","doi-asserted-by":"publisher","first-page":"3843","DOI":"10.3390\/ijms23073843","volume":"23","author":"Y Wu","year":"2022","unstructured":"Wu Y, Zhang X, Fu Y, Liu J, Xue Y, Liu Q (2022) Progesterone can directly inhibit the life activities of Toxoplasma gondii in vitro through the progesterone receptor membrane component (PGRMC). Int J Mol Sci 23(7):3843. https:\/\/doi.org\/10.3390\/ijms23073843","journal-title":"Int J Mol Sci"},{"issue":"12","key":"8079_CR26","doi-asserted-by":"publisher","first-page":"2747","DOI":"10.1084\/jem.20091703","volume":"206","author":"M Yamamoto","year":"2009","unstructured":"Yamamoto M et al (2009) A single polymorphic amino acid on Toxoplasma gondii kinase ROP16 determines the direct and strain-specific activation of Stat3. J Exp Med 206(12):2747\u20132760. https:\/\/doi.org\/10.1084\/jem.20091703","journal-title":"J Exp Med"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-023-08079-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00436-023-08079-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-023-08079-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T10:05:37Z","timestamp":1707127537000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00436-023-08079-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,14]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["8079"],"URL":"https:\/\/doi.org\/10.1007\/s00436-023-08079-9","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,14]]},"assertion":[{"value":"26 August 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 November 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 December 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study protocol and all associated animal studies were approved by the Animal Care and Use Committee of Shandong Agricultural University (approval number: SDAUA-2021\u2013045).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"All authors consent to participate in this publication.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"All authors consent to publish the manuscript.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"45"}}