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Some reports suggest that these reptile ticks bite and attach to humans via accidental parasitism and transmit disease, with the tick <jats:italic>Bothriocroton hydrosauri<\/jats:italic> known to transmit <jats:italic>Rickettsia honei<\/jats:italic> or Flinders Island Spotted Fever <jats:italic>Rickettsia<\/jats:italic> to humans. This descriptive study aims to identify the ticks collected from wild reptiles submitted to veterinary clinics and captured by snake rescuers from New South Wales (NSW), Australia, and detect the presence of tick-borne bacterial DNA using quantitative polymerase chain reaction (qPCR) to detect <jats:italic>Rickettsia<\/jats:italic> spp. and <jats:italic>Bartonella<\/jats:italic> spp. and conventional nested-PCR to detect <jats:italic>Borrelia<\/jats:italic> spp. Morphological identification revealed ticks removed from one eastern blue-tongued lizard (<jats:italic>Tiliqua scincoides scincoides<\/jats:italic>) from North-Eastern NSW (Lismore), one eastern blue-tongued lizard from the Greater Sydney area (Canley Heights), one diamond python (<jats:italic>Morelia spilota spilota<\/jats:italic>) from the Greater Sydney area (Woronora Heights) and one red-bellied black snake (<jats:italic>Pseudechis porphyriacus<\/jats:italic>) from the Greater Sydney Area (Cronulla) in New South Wales were <jats:italic>Amblyomma moreliae<\/jats:italic>. No ticks were positive for <jats:italic>Bartonella<\/jats:italic> spp. and <jats:italic>Borrelia<\/jats:italic> spp. DNA using real-time PCR targeting <jats:italic>ssr<\/jats:italic>A gene and nested PCR targeting <jats:italic>Borrelia<\/jats:italic>-specific 16S rRNA gene, respectively. Real-time PCR targeting <jats:italic>glt<\/jats:italic>A, <jats:italic>omp<\/jats:italic>A, <jats:italic>omp<\/jats:italic>B and 17kDa gene of <jats:italic>Rickettsia<\/jats:italic> spp. revealed 14 out of 16 ticks were positive. The undescribed <jats:italic>Rickettsia<\/jats:italic> sp. DNA was identical to that previously recovered from reptile ticks in Australia and closely related to <jats:italic>Rickettsia tamurae<\/jats:italic> and <jats:italic>Rickettsia monacensis,<\/jats:italic> both of which are aetiologic pathogens of the Spotted Fever Group Rickettsiosis (SFGR). These results accentuate the ongoing need for increased study efforts to understand zoonotic potential of bacteria from reptile ticks and the tick-reptile-human relationship.<\/jats:p>","DOI":"10.1007\/s00436-023-08108-7","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T07:02:43Z","timestamp":1704783763000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Detection of tick-borne bacterial DNA (Rickettsia sp.) in reptile ticks Amblyomma moreliae from New South Wales, Australia"],"prefix":"10.1007","volume":"123","author":[{"given":"Michelle Misong","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenn","family":"Shea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"\u0160lapeta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,9]]},"reference":[{"issue":"1","key":"8108_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.11646\/zootaxa.5253.1.1","volume":"5253","author":"SC Barker","year":"2023","unstructured":"Barker SC, Barker D (2023) Ticks of Australasia: 125 species of ticks in and around Australia. 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