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The safety, immunostimulatory, and antimicrobial properties of alginate nanoparticle formulation (Alg-NP) highlight its potential as an oral therapy against acute toxoplasmosis. In the current study, Alg-NP was formulated and characterized and then assessed for its anti-Toxoplasma effects using parasitological, ultrastructural, immunological, and histopathological studies. Treatment with Alg-NP significantly prolonged mice survival and reduced the parasite burden in both peritoneal fluid and tissue impression smears. In addition, it altered parasite viability and caused severe tachyzoite deformities as evidenced by ultrastructural studies. Alg-NP induced high levels of serum IFN-\u03b3 in infected mice with significant amelioration in histopathological changes in both hepatic and splenic tissue sections. In conclusion, Alg-NP could be considered a promising therapeutic agent against acute murine toxoplasmosis, and owing to its safety, it could potentially be enlisted for human use.<\/jats:p>","DOI":"10.1007\/s00436-024-08307-w","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T06:03:31Z","timestamp":1722924211000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Therapeutic potential of oral alginate nanoparticles against experimental toxoplasmosis"],"prefix":"10.1007","volume":"123","author":[{"given":"Hoda A.","family":"Rashed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amany","family":"Abdel-Bary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Elmorsy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,8,6]]},"reference":[{"key":"8307_CR1","doi-asserted-by":"publisher","first-page":"108548","DOI":"10.1016\/j.exppara.2023.108548","volume":"250","author":"Q AlGabbani","year":"2023","unstructured":"AlGabbani Q (2023) Nanotechnology: a promising strategy for the control of parasitic infections. 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