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Cimicid swallow bugs specifically target swallow birds (Hirundinidae) and their nestlings in infested nests. Bugs of the genus <jats:italic>Oeciacus<\/jats:italic> are commonly found in mud nests of swallows and martins, while they rarely visit the homes of humans. Although\u2014unlike other cimicid species\u2014the house martin bug <jats:italic>Oeciacus hirundinis<\/jats:italic> has never been reported as a vector of zoonotic pathogens, its possible role in arbovirus circulation in continental Europe is unclear. Samples of <jats:italic>O. hirundinis<\/jats:italic> were therefore collected from abandoned house martin (<jats:italic>Delichon urbicum<\/jats:italic>) nests in southern Moravia (Czech Republic) during the 2021\/2022 winter season and checked for alpha-, flavi- and bunyaviruses by RT-PCR. Of a total of 96 pools consisting of three adult bugs each, one pool tested positive for Usutu virus (USUV)-RNA. Phylogenetic analysis showed that the virus strain was closely related to Italian and some Central European strains and corresponded to USUV lineage 5. The detection of USUV in <jats:italic>O. hirundinis<\/jats:italic> during wintertime in the absence of swallows raises the question for a possible role of this avian ectoparasite in virus overwintering in Europe.<\/jats:p>","DOI":"10.1007\/s00436-024-08325-8","type":"journal-article","created":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T04:02:06Z","timestamp":1724126526000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of Usutu virus in a house martin bug Oeciacus hirundinis (Hemiptera: Cimicidae): implications for virus overwintering in a temperate zone"],"prefix":"10.1007","volume":"123","author":[{"given":"Silvie","family":"\u0160ikutov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Mendel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krist\u00edna","family":"Mravcov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romana","family":"Kej\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zden\u011bk","family":"Hub\u00e1lek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helge","family":"Kampen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivo","family":"Rudolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,8,20]]},"reference":[{"key":"8325_CR1","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/j.virol.2004.08.005","volume":"328","author":"T Bakonyi","year":"2004","unstructured":"Bakonyi T, Gould EA, Kolodziejek J, Weissenb\u00f6ck H, Nowotny N (2004) Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001: comparison with the South African strain SAAR-1776 and other flaviviruses. 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