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Since the human parasites are not viable in immune-competent mice, animal models have been developed, among them <jats:italic>Litomosoides sigmodontis<\/jats:italic> which permits a complete life cycle in BALB\/c mice, including the development of patent infections with circulating microfilariae (Mf, the worm\u2019s offspring). To investigate the immunomodulatory properties of helminths in vitro, antigenic extracts can be prepared from different life cycle stages of the <jats:italic>L. sigmodontis<\/jats:italic> model, including adult worms, but the methods to prepare these antigens differ between research groups. This study analyzed whether different centrifugation methods during the preparation of an antigenic extract, the gender of used worms, or the different fractions (soluble or pellet) altered filarial-specific CD4<jats:sup>+<\/jats:sup> T\u00a0cell responses. These cells were isolated from pre-patent or patent\/chronic infected mice, hence those without and those with Mf, respectively. Ex vivo immune responses were compared at these two different time points of the infection as well as the parasitic parameters. Worm burden and cell infiltration were elevated in the thoracic cavity (TC) and draining mediastinal lymph nodes at the pre-patent stage. Within the TC, eosinophils were significantly up-regulated at the earlier time point of infection which was further reflected by the eosinophil-related eotaxin-1 levels. Regarding the production of cytokines by re-stimulated CD4<jats:sup>+<\/jats:sup> T cells in the presence of different antigen preparations, cytokine levels were comparable for all used extracts. Our data show that immune responses differ between pre-patent and patent filarial infection, but not in response to the different antigenic extracts themselves.<\/jats:p>","DOI":"10.1007\/s00436-024-08365-0","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T05:02:24Z","timestamp":1729573344000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differences of in vitro immune responses between patent and pre-patent Litomosoides sigmodontis\u2013infected mice are independent of the filarial antigenic stimulus used"],"prefix":"10.1007","volume":"123","author":[{"given":"Kathrin","family":"Arndts","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Wiszniewsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna-Lena","family":"Neumann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Wiszniewsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gnatoulma","family":"Katawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achim","family":"Hoerauf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura E.","family":"Layland-Heni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Ritter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc P.","family":"H\u00fcbner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,10,22]]},"reference":[{"issue":"6","key":"8365_CR1","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1179\/136485910X12786389891407","volume":"104","author":"T Adjobimey","year":"2010","unstructured":"Adjobimey T, Hoerauf A (2010) Induction of immunoglobulin G4 in human filariasis: an indicator of immunoregulation. 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