{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:14:58Z","timestamp":1790381698304,"version":"4.1.0"},"reference-count":81,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":20,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais , Brasil","award":["APQ-00968-21"],"award-info":[{"award-number":["APQ-00968-21"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais , Brasil","award":["APQ-00968-21"],"award-info":[{"award-number":["APQ-00968-21"]}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1007\/s00436-025-08503-2","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T05:19:20Z","timestamp":1747804760000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Exploring real-time PCR techniques for diagnosing leishmaniasis: key insights from a systematic review"],"prefix":"10.1007","volume":"124","author":[{"given":"Jos\u00e9 Bryan","family":"Rihs","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Teixeira","family":"Vilela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janete Soares Coelho","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio","family":"Caldas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Souza","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Paulo Gomes","family":"Mol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"issue":"14","key":"8503_CR1","doi-asserted-by":"publisher","first-page":"1891","DOI":"10.1017\/s0031182014001280","volume":"141","author":"ER Adams","year":"2014","unstructured":"Adams ER, Gomez MA, Scheske L, Rios R, Marquez R, Cossio A, Albertini A, Schalling H, Saravia NG (2014) Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR. Parasitology 141(14):1891\u20131897. https:\/\/doi.org\/10.1017\/s0031182014001280","journal-title":"Parasitology"},{"issue":"7","key":"8503_CR2","doi-asserted-by":"publisher","first-page":"e00386","DOI":"10.1128\/jcm.00386-18","volume":"56","author":"ER Adams","year":"2018","unstructured":"Adams ER, Schoone G, Versteeg I, Gomez MA, Diro E, Mori Y, Perlee D, Downing T, Saravia N, Assaye A, Hailu A, Albertini A, Ndungu JM, Schalling H (2018) Development and evaluation of a novel loop-mediated isothermal amplification assay for diagnosis of cutaneous and visceral leishmaniasis. J Clin Microbiol 56(7):e00386-18. https:\/\/doi.org\/10.1128\/jcm.00386-18","journal-title":"J Clin Microbiol"},{"key":"8503_CR3","doi-asserted-by":"publisher","unstructured":"Arenas R, Torres-Guerrero E, Quintanilla-Cedillo MR, Ruiz-Esmenjaud J (2017) Leishmaniasis: a review. F1000Res, 6, 750. https:\/\/doi.org\/10.12688\/2Ff1000research.11120.1.","DOI":"10.12688\/2Ff1000research.11120.1"},{"issue":"1","key":"8503_CR4","doi-asserted-by":"publisher","first-page":"24","DOI":"10.4269\/ajtmh.16-84256","volume":"96","author":"N Aronson","year":"2017","unstructured":"Aronson N, Herwaldt BL, Libman M, Pearson R, Lopez-Velez R, Weina P, Carvalho E, Efros M, Jer\u00f4nimo S, Magil A (2017) Diagnosis and treatment of leishmaniasis: Clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH). Am J Trop Med Hyg 96(1):24. https:\/\/doi.org\/10.4269\/ajtmh.16-84256","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"8503_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-018-2717-8","volume":"11","author":"C Attipa","year":"2018","unstructured":"Attipa C, Solano-Gallego L, Papasouliotis K, Soutter F, Morris D, Helps C, Carver S, Tasker S (2018) Association between Canine Leishmaniosis and Ehrlichia canis co-infection: a prospective case-control study. Parasit Vectors 11(1):1\u20139. https:\/\/doi.org\/10.1186\/s13071-018-2717-8","journal-title":"Parasit Vectors"},{"issue":"1\u20132","key":"8503_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.vetpar.2013.03.010","volume":"195","author":"VS Belo","year":"2013","unstructured":"Belo VS, Struchiner CJ, Werneck GL, Barbosa DS, de Oliveira RB, Neto RGT, da Silva ES (2013) A systematic review and meta-analysis of the factors associated with Leishmania infantum infection in dogs in Brazil. Vet Parasitol 195(1\u20132):1\u201313. https:\/\/doi.org\/10.1016\/j.vetpar.2013.03.010","journal-title":"Vet Parasitol"},{"key":"8503_CR7","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.exppara.2017.04.004","volume":"177","author":"JC Benassi","year":"2017","unstructured":"Benassi JC, Benvenga GU, Ferreira HL, Pereira VF, Keid LB, Soares R, Oliveira TMFS (2017) Detection of Leishmania infantum DNA in conjunctival swabs of cats by quantitative real-time PCR. Exp Parasitol 177:93\u201397. https:\/\/doi.org\/10.1016\/j.exppara.2017.04.004","journal-title":"Exp Parasitol"},{"issue":"5","key":"8503_CR8","doi-asserted-by":"publisher","first-page":"e188","DOI":"10.1111\/jdv.15440","volume":"33","author":"JO Bergmann","year":"2019","unstructured":"Bergmann JO, dos Santos J\u00fanior ACM, Sevilha-Santos L, Medeiros-Silva V, Pompeu CB, Arabi AYM, Marques DS, de Carvalho BCV, Nitz N, Gomes CM, de Lima BD, Sampaio RNR (2019) Accuracy of a TaqMan-based real-time polymerase chain reaction combined to a Novy-MacNeal-Nicolle medium culture for the diagnosis of American Tegumentary Leishmaniasis. J Eur Acad Dermatol Venereol 33(5):e188\u2013e190. https:\/\/doi.org\/10.1111\/jdv.15440","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"8503_CR9","doi-asserted-by":"publisher","DOI":"10.1590\/S1678-9946201759038","volume":"59","author":"SM Boni","year":"2017","unstructured":"Boni SM, Oyafuso LK, Soler RC, Lindoso JAL (2017) Efficiency of noninvasive sampling methods (swab) together with polymerase chain reaction (PCR) for diagnosing American Tegumentary Leishmaniasis. Rev Inst Med Trop Sao Paulo 59:e38. https:\/\/doi.org\/10.1590\/S1678-9946201759038","journal-title":"Rev Inst Med Trop Sao Paulo"},{"issue":"2","key":"8503_CR10","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1007\/s15010-022-01943-3","volume":"51","author":"M Busutti","year":"2023","unstructured":"Busutti M, Deni A, De Pascali AM, Ortalli M, Attard L, Granozzi B, Fabbrizio B, La Man\u00e1 G, Comai G, Varani S (2023) Updated diagnosis and graft involvement for Visceral Leishmaniasis in kidney transplant recipients: a case report and literature review. Infection 51(2):507\u2013518. https:\/\/doi.org\/10.1007\/s15010-022-01943-3","journal-title":"Infection"},{"issue":"5","key":"8503_CR11","doi-asserted-by":"publisher","first-page":"1683","DOI":"10.1007\/s00436-020-06654-y","volume":"119","author":"S Caldas","year":"2020","unstructured":"Caldas S, Marcelino AP, Faria G, de Oliveira SF, Ataide ACZ, Cunha LM, Bahia MTB, Paz GF, Gontijo CMF (2020) Visceral Leishmaniasis: a practical strategy for quantitative molecular diagnosis in naturally infected dogs. Parasitol Res 119(5):1683\u20131690. https:\/\/doi.org\/10.1007\/s00436-020-06654-y","journal-title":"Parasitol Res"},{"issue":"6","key":"8503_CR12","doi-asserted-by":"publisher","first-page":"1443","DOI":"10.1590\/1678-4162-9053","volume":"69","author":"MP Campos","year":"2017","unstructured":"Campos MP, Madeira MF, Silva DA, Solc\u00e0 MS, Esp\u00edndola OM, J\u00fanior AAVM, Figueiredo FB (2017) Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of Canine Visceral Leishmaniasis. Arq Bras Med Vet Zootec 69(6):1443\u20131450. https:\/\/doi.org\/10.1590\/1678-4162-9053","journal-title":"Arq Bras Med Vet Zootec"},{"key":"8503_CR13","doi-asserted-by":"crossref","unstructured":"Castellanos-Gonzalez A, Saldarriaga AO, Tartaglino L, Gacek R, Temple E, Sparks H, Melby PC, Travi BL (2015) A novel molecular test to diagnose Canine Visceral Leishmaniasis at the point of care. American Journal of Tropical Medicine and Hygiene 93(5):970\u2013975","DOI":"10.4269\/ajtmh.15-0145"},{"issue":"4","key":"8503_CR14","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1016\/j.diagmicrobio.2013.10.015","volume":"78","author":"AL Carvalho Ferreira","year":"2014","unstructured":"Carvalho Ferreira AL, Carregal VM, De Almeida FS, Leite RS, de Andrade ASR (2014) Detection of Leishmania infantum in 4 different dog samples by real-time PCR and ITS-1 nested PCR. Diagn Microbiol Infect Dis 78(4):418\u2013421. https:\/\/doi.org\/10.1016\/j.diagmicrobio.2013.10.015","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"7","key":"8503_CR15","doi-asserted-by":"publisher","first-page":"865","DOI":"10.3390\/pathogens10070865","volume":"10","author":"G Castelli","year":"2021","unstructured":"Castelli G, Bruno F, Reale S, Catanzaro S, Valenza V, Vitale F (2021) Diagnosis of leishmaniasis: quantification of parasite load by a real-time PCR assay with high sensitivity. Pathogens 10(7):865. https:\/\/doi.org\/10.3390\/pathogens10070865","journal-title":"Pathogens"},{"issue":"1","key":"8503_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-014-0460-3","volume":"7","author":"M Ceccarelli","year":"2014","unstructured":"Ceccarelli M, Galluzzi L, Sisti D, Bianchi B, Magnani M (2014) Application of qPCR in conjunctival swab samples for the evaluation of Canine Leishmaniasis in borderline cases or disease relapse and correlation with clinical parameters. Parasit Vectors 7(1):1\u201312. https:\/\/doi.org\/10.1186\/s13071-014-0460-3","journal-title":"Parasit Vectors"},{"issue":"12","key":"8503_CR17","doi-asserted-by":"publisher","first-page":"2006","DOI":"10.3390\/microorganisms8122006","volume":"8","author":"M Ceccarelli","year":"2020","unstructured":"Ceccarelli M, Buffi G, Diotallevi A, Andreoni F, Bencardino D, Vitale F, Castelli G, Bruno F, Magnani M, Galluzzi L (2020) Evaluation of a kDNA-based qPCR assay for the detection and quantification of old world leishmania species. Microorganisms 8(12):2006. https:\/\/doi.org\/10.3390\/microorganisms8122006","journal-title":"Microorganisms"},{"key":"8503_CR18","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.exppara.2019.05.006","volume":"203","author":"JLL Celeste","year":"2019","unstructured":"Celeste JLL, Caldeira RL, Pires SF, Silveira KD, Soares RP, de Andrade HM (2019) Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Leishmania amazonensis in skin samples. Exp Parasitol 203:23\u201329. https:\/\/doi.org\/10.1016\/j.exppara.2019.05.006","journal-title":"Exp Parasitol"},{"issue":"3","key":"8503_CR19","doi-asserted-by":"publisher","first-page":"e03354","DOI":"10.1128\/spectrum.03354-22","volume":"11","author":"C Chicharro","year":"2013","unstructured":"Chicharro C, Nieto J, Miguela\u00f1ez S, Garcia E, Ortega S, Pe\u00f1a A, Rubio JM, Flores-Ch\u00e1vez M (2013) Molecular diagnosis of leishmaniasis in Spain: development and validation of ready-to-use gel-form nested and real-time PCRs to detect Leishmania spp. Microbiology Spectrum 11(3):e03354-e3422. https:\/\/doi.org\/10.1128\/spectrum.03354-22","journal-title":"Microbiology Spectrum"},{"issue":"2","key":"8503_CR20","doi-asserted-by":"publisher","first-page":"95","DOI":"10.3390\/tropicalmed5020095","volume":"5","author":"R Chowdhury","year":"2020","unstructured":"Chowdhury R, Ghosh P, Khan MAA, Hossain F, Faisal K, Nath R, Baker J, El Wahed AA, Maruf S, Nath P, Ghosh D, Masud-Ur-Rashid MD, Utba Bin Rashid MD, Duthie MS, Mondal D (2020) Evaluation of rapid extraction methods coupled with a recombinase polymerase amplification assay for point-of-need diagnosis of post-kala-azar dermal leishmaniasis. Tropical Medicine and Infectious Disease 5(2):95. https:\/\/doi.org\/10.3390\/tropicalmed5020095","journal-title":"Tropical Medicine and Infectious Disease"},{"issue":"12","key":"8503_CR21","doi-asserted-by":"publisher","first-page":"5197","DOI":"10.1128\/AAC.00866-09","volume":"53","author":"RI da Luz","year":"2009","unstructured":"da Luz RI, Vermeersch M, Dujardin JC, Cos P, Maes L (2009) In vitro sensitivity testing of Leishmania clinical field isolates: preconditioning of promastigotes enhances infectivity for macrophage host cells. Antimicrob Agents Chemother 53(12):5197\u20135203. https:\/\/doi.org\/10.1128\/AAC.00866-09","journal-title":"Antimicrob Agents Chemother"},{"issue":"4","key":"8503_CR22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002150","volume":"7","author":"SA de Ferreira","year":"2013","unstructured":"de Ferreira SA, Almeida GG, de Silva SO, Vogas GP, Fujiwara RT, de Andrade ASR, Melo MN (2013) Nasal, oral and ear swabs for Canine Visceral Leishmaniasis diagnosis: New practical approaches for detection of Leishmania infantum DNA. PLoS Negl Trop Dis 7(4):e2150. https:\/\/doi.org\/10.1371\/journal.pntd.0002150","journal-title":"PLoS Negl Trop Dis"},{"key":"8503_CR23","doi-asserted-by":"publisher","unstructured":"de Morais RCS, Melo de MGN, Goes de TC, Pessoa e Silva R, de Morais de RF, Guerra JAO, Brito de MEF, Brand\u00e3o-Filho SP, Cavalcante MP (2020) Duplex qPCR for Leishmania species identification using lesion imprint on filter paper. Exp Parasitol 219: 108019. https:\/\/doi.org\/10.1016\/j.exppara.2020.108019","DOI":"10.1016\/j.exppara.2020.108019"},{"issue":"12","key":"8503_CR24","doi-asserted-by":"publisher","first-page":"2953","DOI":"10.1590\/s0102-311x2008001200026","volume":"24","author":"OC Di Lorenzo","year":"2008","unstructured":"Di Lorenzo OC, Morais MHF, Machado-Coelho GLL (2008) Visceral Leishmaniasis in large brazilian cities: challenges for control. SciELO Public Health 24(12):2953\u20132958. https:\/\/doi.org\/10.1590\/s0102-311x2008001200026","journal-title":"SciELO Public Health"},{"issue":"6","key":"8503_CR25","doi-asserted-by":"publisher","first-page":"465","DOI":"10.4269\/ajtmh.16-84256","volume":"65","author":"KK Dixit","year":"2020","unstructured":"Dixit KK, Singh R, Salotra P (2020) Advancement in molecular diagnosis of post kala-azar dermal leishmaniasis. Indian J Dermatol 65(6):465. https:\/\/doi.org\/10.4269\/ajtmh.16-84256","journal-title":"Indian J Dermatol"},{"key":"8503_CR26","doi-asserted-by":"publisher","unstructured":"Dixit KK, Ramesh V, Gupta R, Negi NS, Singh R, Salotra P (2021) Real-time fluorimetry loop-mediated isothermal amplification for diagnosis of leishmaniasis and as a tool for assessment of cure for Post\u2013Kala-Azar Dermal Leishmaniasis. Am J Trop Med Hyg, 104(6): 2097. https:\/\/doi.org\/10.4269\/2Fajtmh.20-1057.","DOI":"10.4269\/2Fajtmh.20-1057"},{"issue":"9","key":"8503_CR27","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00132-21","volume":"59","author":"KK Dixit","year":"2021","unstructured":"Dixit KK, Ramesh V, Upadhyay S, Singh AK, Singh OP, Sundar S, Singh R, Salotra P (2021b) Utility of Blood as the Clinical Specimen for the Molecular Diagnosis of Post-Kala-Azar Dermal Leishmaniasis. J Clin Microbiol 59(9):e0013221. https:\/\/doi.org\/10.1128\/jcm.00132-21","journal-title":"J Clin Microbiol"},{"key":"8503_CR28","doi-asserted-by":"publisher","unstructured":"Eroglu F, Uzun S, Koltas IS (2014) Comparison of clinical samples and methods in chronic Cutaneous Leishmaniasis. Am J Trop Med Hyg, 91(5), 895. https:\/\/doi.org\/10.4269\/2Fajtmh.13-0582.","DOI":"10.4269\/2Fajtmh.13-0582"},{"issue":"10","key":"8503_CR29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008750","volume":"14","author":"CPB Filgueira","year":"2020","unstructured":"Filgueira CPB, Moreira OC, Cantanh\u00eade LM, de Farias HMT, Porrozzi R, Britto C, Boit\u00e9 MC, Cupolillo E (2020) Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis. PLoS Negl Trop Dis 14(10):e0008750. https:\/\/doi.org\/10.1371\/journal.pntd.0008750","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"8503_CR30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2017.01907","volume":"11","author":"L Galluzzi","year":"2018","unstructured":"Galluzzi L, Ceccarelli M, Diotallevi A, Menotta M, Magnani M (2018) Real-time PCR applications for diagnosis of leishmaniasis. Parasit Vectors 11(1):1\u201313. https:\/\/doi.org\/10.3389\/fmicb.2017.01907","journal-title":"Parasit Vectors"},{"issue":"3","key":"8503_CR31","doi-asserted-by":"publisher","first-page":"588","DOI":"10.3390\/microorganisms9030588","volume":"9","author":"P Ghosh","year":"2021","unstructured":"Ghosh P, Sharma A, Bhattarai NR, Abhishek K, Nisansala T, Kumar A, B\u00f6hlken-Fascher S, Chowdhury R, Khan MDAA, Faisal K, Hossain F, Uddin MRR, Rashid MDUU, Maruf S, Rai K, Sooriyaarachchi M, Abhayarathna WLK, Karki P, Kumar S, Ranasinghe S, Khanal B, Satyabrata R, Das P, Mondal D, El Wahed AA (2021) A multi-country, single-blinded, phase 2 study to evaluate a point-of-need system for rapid detection of leishmaniasis and its implementation in endemic settings. Microorganisms 9(3):588. https:\/\/doi.org\/10.3390\/microorganisms9030588","journal-title":"Microorganisms"},{"issue":"2","key":"8503_CR32","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1128\/jcm.01954-16","volume":"55","author":"CM Gomes","year":"2017","unstructured":"Gomes CM, Cesetti MV, De Paula NA, Vernal S, Gupta G, Sampaio RNR, Roselino AM (2017) Field validation of SYBR Green- and TaqMan-based real-time PCR using biopsy and swab samples to diagnose American Tegumentary Leishmaniasis in an area where Leishmania (Viannia) braziliensis is endemic. J Clin Microbiol 55(2):526\u2013534. https:\/\/doi.org\/10.1128\/jcm.01954-16","journal-title":"J Clin Microbiol"},{"key":"8503_CR33","unstructured":"Guti\u00e9rrez-Ocampo E, Villamizar-Pe\u00f1a R, Cortes-Bonilla I, Garc\u00eda-Zuluaga LM, Holguin-Rivera Y, Ospina-Arzuaga HD, Trujilo MCC, Trejos-Mendonza AE, Perez-Vargas S, Arteaga-Livias K, Zambrano LI, Bonilla-Aldana DK, Perez-Garcia LA, Hern\u00e1ndez-Pereira CE, Rodriguez-Morales AJ, Paniz-Mondolfi A, Delgado O (2021) Human Visceral Leishmaniasis prevalence by different diagnostic methods in Latin America: a systematic review and meta-analysis. Infez Med, 29(2), 199\u2013208. https:\/\/europepmc.org\/article\/med\/34061784."},{"issue":"9","key":"8503_CR34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185606","volume":"12","author":"F Hossain","year":"2017","unstructured":"Hossain F, Ghosh P, Khan MAA, Duthie MS, Vallur AC, Picone A, Howard RF, Reed SG, Mondal D (2017) Real-time PCR in detection and quantitation of Leishmania donovani for the diagnosis of Visceral Leishmaniasis patients and the monitoring of their response to treatment. PLoS ONE 12(9):e0185606. https:\/\/doi.org\/10.1371\/journal.pone.0185606","journal-title":"PLoS ONE"},{"key":"8503_CR35","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.670759","volume":"11","author":"F Hossain","year":"2021","unstructured":"Hossain F, Picado A, Owen SI, Ghosh P, Chowdhury R, Maruf S, Khan MDAA, Rashid MDU, Nath R, Baker J, Ghosh D, Adams ER, Duthie MS, Hossain MDS, Basher A, Nath P, Aktar F, Cruz I, Mondal D (2021) Evaluation of Loopamp\u2122 Leishmania detection kit and Leishmania antigen ELISA for post-elimination detection and management of Visceral Leishmaniasis in Bangladesh. Front Cell Infect Microbiol 11:670759. https:\/\/doi.org\/10.3389\/fcimb.2021.670759","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"8503_CR36","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.pt.2019.10.005","volume":"36","author":"AV Ibarra-Meneses","year":"2020","unstructured":"Ibarra-Meneses AV, Moreno J, Carrillo E (2020) New strategies and biomarkers for the control of Visceral Leishmaniasis. Trends Parasitol 36(1):29\u201338. https:\/\/doi.org\/10.1016\/j.pt.2019.10.005","journal-title":"Trends Parasitol"},{"key":"8503_CR37","doi-asserted-by":"publisher","unstructured":"Ibarra-Meneses AV, Chicharro C, S\u00e1nchez C, Garc\u00eda E, Ortega S, Ndung\u2019u JM, Moreno J, Cruz I, Carrillo E (2021) Loop-mediated isothermal amplification allows rapid, simple and accurate molecular diagnosis of Human Cutaneous and Visceral Leishmaniasis caused by Leishmania infantum when compared to PCR. Microorganisms, 9(3), 610, https:\/\/doi.org\/10.3390\/microorganisms9030610","DOI":"10.3390\/microorganisms9030610"},{"issue":"23\u201324","key":"8503_CR38","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1016\/j.drudis.2013.08.005","volume":"18","author":"K Jain","year":"2013","unstructured":"Jain K, Jain NK (2013) Novel therapeutic strategies for treatment of Visceral Leishmaniasis. Drug Discov Today 18(23\u201324):1272\u20131281. https:\/\/doi.org\/10.1016\/j.drudis.2013.08.005","journal-title":"Drug Discov Today"},{"issue":"6","key":"8503_CR39","doi-asserted-by":"publisher","first-page":"1826","DOI":"10.1128\/jcm.00208-13","volume":"51","author":"M Jara","year":"2013","unstructured":"Jara M, Adaui V, Valencia BM, Martinez D, Alba M, Castrillon C, Cruz M, Cruz I, Van der Auwera G, Llanos-Cuentas A, Dujardin JC, Arevalo J (2013) Real-time PCR assay for detection and quantification of Leishmania (Viannia) organisms in skin and mucosal lesions: Exploratory study of parasite load and clinical parameters. J Clin Microbiol 51(6):1826\u20131833. https:\/\/doi.org\/10.1128\/jcm.00208-13","journal-title":"J Clin Microbiol"},{"key":"8503_CR40","doi-asserted-by":"publisher","unstructured":"Larson M, Toepp A, Scott B, EPID:158, Kurtz M, Fowler H, Esfandiari J, Howard RF, Vallur ACC, Duthie MS, Petersen C (2017) Semi-quantitative measurement of asymptomatic L. infantum infection and symptomatic Visceral Leishmaniasis in dogs using Dual-Path Platform\u00ae CVL. Appl Microbiol Biotechnol, 101(1), 381\u201390. https:\/\/doi.org\/10.1007\/s00253-016-7925-6.","DOI":"10.1007\/s00253-016-7925-6"},{"issue":"3\u20134","key":"8503_CR41","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.exppara.2017.04.004","volume":"170","author":"RS Leite","year":"2010","unstructured":"Leite RS, Ferreira AS, Ituassu LT, de Melo MN, de Andrade ASR (2010) PCR diagnosis of Visceral Leishmaniasis in asymptomatic dogs using conjunctival swab samples. Vet Parasitol 170(3\u20134):201\u2013206. https:\/\/doi.org\/10.1016\/j.exppara.2017.04.004","journal-title":"Vet Parasitol"},{"key":"8503_CR42","doi-asserted-by":"publisher","unstructured":"Le\u00f3n CM, Mu\u00f1oz M, Hern\u00e1ndez C, Ayala MS, Fl\u00f3rez C, Teher\u00e1n A, Cubides JR, Ram\u00edrez JD (2017) Analytical performance of four polymerase chain reaction (PCR) and real time PCR (qPCR) assays for the detection of six Leishmania species DNA in Colombia. Front Microbiol, 8(OCT), 1907. https:\/\/doi.org\/10.3389\/fmicb.2017.01907.","DOI":"10.3389\/fmicb.2017.01907"},{"issue":"4","key":"8503_CR43","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1371\/journal.pone.0103635","volume":"30","author":"T Lima","year":"2019","unstructured":"Lima T, Mart\u00ednez-Sogues L, Montserrat-Sangr\u00e0 S, Solano-Gallego L, Ordeix L (2019) Diagnostic performance of a qPCR for Leishmania on stained cytological specimens and on filter paper impressions obtained from cutaneous lesions suggestive of canine leishmaniosis. Vet Dermatol 30(4):318-e89. https:\/\/doi.org\/10.1371\/journal.pone.0103635","journal-title":"Vet Dermatol"},{"issue":"12","key":"8503_CR44","doi-asserted-by":"publisher","first-page":"2436","DOI":"10.1017\/s0950268817001443","volume":"145","author":"EG Lopes","year":"2017","unstructured":"Lopes EG, Sev\u00e1 ADP, Ferreira F, Nunes CM, Keid LB, Hiramoto RM, Ferreira HL, Oliveira TMFS, Bigoto MFD, Galvis-Ovallos F, Galati EAB, Soares RM (2017) Serological and molecular diagnostic tests for canine visceral leishmaniasis in Brazilian endemic area: one out of five seronegative dogs are infected. Epidemiol Infect 145(12):2436\u20132444. https:\/\/doi.org\/10.1017\/s0950268817001443","journal-title":"Epidemiol Infect"},{"issue":"2","key":"8503_CR45","doi-asserted-by":"publisher","first-page":"43","DOI":"10.3390\/vetsci7020043","volume":"7","author":"MP Maurelli","year":"2020","unstructured":"Maurelli MP, Bosco A, Manzillo VF, Vitale F, Giaquinto D, Ciuca L, Molinaro G, Cringoli G, Oliva G, Rinaldi L, Gizzarelli L (2020) Clinical, molecular and serological diagnosis of canine leishmaniosis: an integrated approach. Veterinary Sciences 7(2):43. https:\/\/doi.org\/10.3390\/vetsci7020043","journal-title":"Veterinary Sciences"},{"issue":"1","key":"8503_CR46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008903","volume":"15","author":"B Merdekios","year":"2021","unstructured":"Merdekios B, Pareyn M, Tadesse D, Eligo N, Kassa M, Jacobs BKM, Leirs H, Van Geertruyden JP, Van Griensven J, Caljon G, Cnops L (2021) Evaluation of conventional and four real-time PCR methods for the detection of Leishmania on field-collected samples in Ethiopia. PLoS Negl Trop Dis 15(1):e0008903. https:\/\/doi.org\/10.1371\/journal.pntd.0008903","journal-title":"PLoS Negl Trop Dis"},{"key":"8503_CR47","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.meegid.2018.05.021","volume":"63","author":"A Mirzaei","year":"2018","unstructured":"Mirzaei A, Ahmadipour F, Cannet A, Marty P, Delaunay P, Perrin P, Dorkeld F, Sereno D, Akhoundi M (2018) Immunodetection and molecular determination of visceral and cutaneous Leishmania infection using patients\u2019 urine. Infect Genet Evol 63:257\u201368. https:\/\/doi.org\/10.1016\/j.meegid.2018.05.021","journal-title":"Infect Genet Evol"},{"issue":"1","key":"8503_CR48","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.exppara.2012.10.017","volume":"133","author":"A Mohammadiha","year":"2013","unstructured":"Mohammadiha A, Mohebali M, Haghighi A, Mahdian R, Abadi AR, Zarei Z, Yeganeh F, Kazemi B, Taghipour M, Akhoundi B (2013) Comparison of real-time PCR and conventional PCR with two DNA targets for detection of Leishmania (Leishmania) infantum infection in human and dog blood samples. Exp Parasitol 133(1):89\u201394. https:\/\/doi.org\/10.1016\/j.exppara.2012.10.017","journal-title":"Exp Parasitol"},{"key":"8503_CR49","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.micpath.2018.10.036","volume":"126","author":"FM Monteiro","year":"2019","unstructured":"Monteiro FM, Machado AS, Rocha-Silva F, Assun\u00e7\u00e3o CB, Graciele-Melo C, Costa LE, Portela AS, Coelho EAF, de Figueiredo SM, Caligiorne RB (2019) Canine Visceral Leishmaniasis: Detection of Leishmania spp. genome in peripheral blood of seropositive dogs by real-time Polymerase Chain Reaction (rt-PCR). Microb Pathog 126:263\u2013268. https:\/\/doi.org\/10.1016\/j.micpath.2018.10.036","journal-title":"Microb Pathog"},{"issue":"1","key":"8503_CR50","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-016-1572-8","volume":"9","author":"D Mondal","year":"2016","unstructured":"Mondal D, Ghosh P, Khan MAA, Hossain F, B\u00f6hlken-Fascher S, Matlashewski G, Kroeger A, Olliaro P, El Wahed AA (2016) Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay. Parasit Vectors 9(1):1\u20138. https:\/\/doi.org\/10.1186\/s13071-016-1572-8","journal-title":"Parasit Vectors"},{"key":"8503_CR51","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.actatropica.2017.09.020","volume":"184","author":"OC Moreira","year":"2018","unstructured":"Moreira OC, Yadon ZE, Cupolillo E (2018) The applicability of real-time PCR in the diagnostic of cutaneous leishmaniasis and parasite quantification for clinical management: Current status and perspectives. Acta Trop 184:29\u201337. https:\/\/doi.org\/10.1016\/j.actatropica.2017.09.020","journal-title":"Acta Trop"},{"key":"8503_CR52","doi-asserted-by":"publisher","unstructured":"Nicolas L, Prina E, Lang T, Milon G (2002) Real-time PCR for detection and quantitation of Leishmania in mouse tissues. J Clin Microbiol, 40(5), 1666\u20139. https:\/\/doi.org\/10.1128\/2FJCM.40.5.1666-1669.2002.","DOI":"10.1128\/2FJCM.40.5.1666-1669.2002"},{"issue":"12","key":"8503_CR53","doi-asserted-by":"publisher","first-page":"e1032","DOI":"10.1016\/j.ijid.2010.06.019","volume":"14","author":"A Pavli","year":"2010","unstructured":"Pavli A, Maltezou HC (2010) Leishmaniasis, an emerging infection in travelers. Int J Infect Dis 14(12):e1032-9. https:\/\/doi.org\/10.1016\/j.ijid.2010.06.019","journal-title":"Int J Infect Dis"},{"issue":"2","key":"8503_CR54","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1016\/j.jiph.2022.12.021","volume":"16","author":"MMC Pederiva","year":"2023","unstructured":"Pederiva MMC, Santos SM, Rivarola LGS, Guerreiro VJ, Lopes KS, Lima J\u00fanior MSC, Neitzke-Abreu HC (2023) Asymptomatic Leishmania infection in humans: a systematic review. J Infect Public Health 16(2):286\u201394. https:\/\/doi.org\/10.1016\/j.jiph.2022.12.021","journal-title":"J Infect Public Health"},{"issue":"10","key":"8503_CR55","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1111\/tmi.13465","volume":"25","author":"HP Pena","year":"2020","unstructured":"Pena HP, Belo VS, Xavier-J\u00fanior JCC, Teixeira-Neto RG, Melo SN, Pereira DA, Fontes IC, Santos IM, Lopes VV, Tafuri WL, Romero GAS, da Silva ES (2020) Accuracy of diagnostic tests for American Tegumentary Leishmaniasis: a systematic literature review with meta-analyses. Tropical Med Int Health 25(10):1168\u20131181. https:\/\/doi.org\/10.1111\/tmi.13465","journal-title":"Tropical Med Int Health"},{"key":"8503_CR56","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/639310","volume":"2014","author":"MR Pereira","year":"2014","unstructured":"Pereira MR, Rocha-Silva F, Graciele-Melo C, Lafuente CR, Magalh\u00e3es T, Caligiorne RB (2014) Comparison between conventional and real-time PCR assays for diagnosis of Visceral Leishmaniasis. Biomed Res Int 2014:639310. https:\/\/doi.org\/10.1155\/2014\/639310","journal-title":"Biomed Res Int"},{"key":"8503_CR57","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.mimet.2016.10.002","volume":"131","author":"R Pessoa-e-Silva","year":"2016","unstructured":"Pessoa-e-Silva R, Trajano-Silva LAM, da Silva MAL, Gon\u00e7alves-de-Albuquerque SC, de Goes TC, de Morais RCS, de Melo FL, de Paiva-Cavalcanti M (2016) Evaluation of urine for Leishmania infantum DNA detection by real-time quantitative PCR. J Microbiol Methods 131:34\u201341. https:\/\/doi.org\/10.1016\/j.mimet.2016.10.002","journal-title":"J Microbiol Methods"},{"issue":"3","key":"8503_CR58","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1590\/S1984-29612013000300005","volume":"22","author":"RAN Ramos","year":"2013","unstructured":"Ramos RAN, Ramos CAN, Santos EMS, Ara\u00fajo FR, Carvalho GA, Faustino MAG, Alves LC (2013) Quantification of Leishmania infantum DNA in the bone marrow, lymph node and spleen of dogs. Rev Bras Parasitol Vet 22(3):346\u201350. https:\/\/doi.org\/10.1590\/S1984-29612013000300005","journal-title":"Rev Bras Parasitol Vet"},{"issue":"3\u20134","key":"8503_CR59","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1016\/j.vetpar.2013.07.006","volume":"197","author":"LES Reis","year":"2013","unstructured":"Reis LES, Coura-Vital W, Roatt BM, Bouillet LEM, Ker HG, Fortes de Brito RC, Resende DM, Carneiro M, Giunchetti RC, Marques MJ, Carneiro CM, Reis AB (2013) Molecular diagnosis of canine visceral leishmaniasis: a comparative study of three methods using skin and spleen from dogs with natural Leishmania infantum infection. Vet Parasitol 197(3\u20134):498\u2013503. https:\/\/doi.org\/10.1016\/j.vetpar.2013.07.006","journal-title":"Vet Parasitol"},{"key":"8503_CR60","doi-asserted-by":"publisher","unstructured":"Rijal S, Sundar C, Mondal D, Das P, Alvar J, Bolaert M (2019) Eliminating visceral leishmaniasis in South Asia: the road ahead. bmj, 364. https:\/\/doi.org\/10.1136\/bmj.k5224.","DOI":"10.1136\/bmj.k5224"},{"key":"8503_CR61","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-016-1412-x","volume":"9","author":"V Rodrigues","year":"2016","unstructured":"Rodrigues V, de CordeiroSilva A, Laforge M, Silvestre R, Estaquier J (2016) Regulation of immunity during visceral Leishmania infection. Parasites & vectors 9:1\u201313. https:\/\/doi.org\/10.1186\/s13071-016-1412-x","journal-title":"Parasites & vectors"},{"key":"8503_CR62","doi-asserted-by":"publisher","unstructured":"Sagi O, Berkowitz A, Codish S, Novack V, Rashti A, Akad F, Shemer-Avni Y (2017) Sensitive molecular diagnostics for cutaneous leishmaniasis. Open Forum Infect Dis, 4(2), ofx037. https:\/\/doi.org\/10.1093\/ofid\/ofx037.","DOI":"10.1093\/ofid\/ofx037"},{"key":"8503_CR63","doi-asserted-by":"publisher","unstructured":"Saldarriaga OA, Castellanos-Gonzalez A, Porrozzi R, Baldeviano GC, Lescano AG, de Los Santos MB, Fern\u00e1ndez OL, Saravia NG, Costa E, Melby PC, Travi BL (2016) An innovative field-applicable molecular test to diagnose cutaneous Leishmania Viannia spp., Infections. PLoS Negl Trop Dis 10(4): e0004638. https:\/\/doi.org\/10.1371\/journal.pntd.0004638.","DOI":"10.1371\/journal.pntd.0004638"},{"issue":"10","key":"8503_CR64","doi-asserted-by":"publisher","first-page":"1887","DOI":"10.3390\/microorganisms10101887","volume":"10","author":"S Scarpini","year":"2022","unstructured":"Scarpini S, Dondi A, Totaro C, Biagi C, Melchionda F, Zama D, Pierantoni L, Gennari M, Campagna C, Prete A, Lanari M (2022) Visceral Leishmaniasis: epidemiology, diagnosis, and treatment regimens in different geographical areas with a focus on pediatrics. Microorganisms 10(10):1887. https:\/\/doi.org\/10.3390\/microorganisms10101887","journal-title":"Microorganisms"},{"issue":"2","key":"8503_CR65","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.cmi.2018.04.025","volume":"25","author":"L Sevilha-Santos","year":"2019","unstructured":"Sevilha-Santos L, dos Santos J\u00fanior ACM, Medeiros-Silva V, Bergmann JO, da Silva EF, Segato LF, \u00c1rabe AYM, de Paula NA, Sampaio RNR, Lima BD, Gomes CM (2019) Accuracy of qPCR for quantifying Leishmania kDNA in different skin layers of patients with American Tegumentary Leishmaniasis. Clin Microbiol Infect 25(2):242\u20137. https:\/\/doi.org\/10.1016\/j.cmi.2018.04.025","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"8503_CR66","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1080\/01652176.2016.1252073","volume":"37","author":"RC Silva","year":"2016","unstructured":"Silva RC, Richini-Pereira VB, Kikuti M, Marson PM, Langoni H (2016) Detection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primers. Veterinary Quarterly 37(1):23\u201330. https:\/\/doi.org\/10.1080\/01652176.2016.1252073","journal-title":"Veterinary Quarterly"},{"issue":"7","key":"8503_CR67","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103635","volume":"9","author":"MDS Solc\u00e0","year":"2014","unstructured":"Solc\u00e0 MDS, Bastos LA, Guedes CES, Bordoni M, Borja LS, Laranjeira DF, Tuy PGSE, Amorim LDAF, Nascimento EG, Oliveira GGS, dos Santos WLC, Fraga DBM, Veras PST (2014) Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis. PLoS ONE 9(7):e103635. https:\/\/doi.org\/10.1371\/journal.pone.0103635","journal-title":"PLoS ONE"},{"issue":"7","key":"8503_CR68","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003936","volume":"9","author":"M Su\u00e1rez","year":"2015","unstructured":"Su\u00e1rez M, Valencia BM, Jara M, Alba M, Boggild AK, Dujardin JC, Llanos-Cuentas A, Arevalo J, Adaui V (2015) Quantification of Leishmania (Viannia) kinetoplast DNA in ulcers of Cutaneous Leishmaniasis reveals inter-site and inter-sampling variability in parasite load. PLoS Negl Trop Dis 9(7):e0003936. https:\/\/doi.org\/10.1371\/journal.pntd.0003936","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"8503_CR69","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1007\/s40291-018-0343-y","volume":"22","author":"S Sundar","year":"2018","unstructured":"Sundar S, Singh OP (2018) Molecular diagnosis of Visceral Leishmaniasis. Mol Diagn Ther 22(4):443\u201357. https:\/\/doi.org\/10.1007\/s40291-018-0343-y","journal-title":"Mol Diagn Ther"},{"issue":"7","key":"8503_CR70","doi-asserted-by":"publisher","first-page":"826","DOI":"10.3390\/pathogens10070826","volume":"10","author":"K Tanida","year":"2021","unstructured":"Tanida K, Balczun C, Hahn A, Veit A, Nickel B, Poppert S, Scheid PL, Hagen RM, Frickmann H, Loderst\u00e4dt U, Tannick E (2021) Comparison of three in-house real PCR assays targeting kinetoplast DNA, the small subunit ribosomal RNA gene and the glucose-6-phosphate isomerase gene for the detection of Leishmania spp. in human serum. Pathogens 10(7):826. https:\/\/doi.org\/10.3390\/pathogens10070826","journal-title":"Pathogens"},{"key":"8503_CR71","doi-asserted-by":"crossref","unstructured":"Thomaz C, de Mello CX, Esp\u00edndola OM, Shubach AO, Quintella LP, de Oliveira RVC, Duarte ACG, Pimentel MIF, Lyra MR, Marzochi MCA (2021) Comparison of parasite load by qPCR and histopathological changes of inner and outer edge of ulcerated cutaneous lesions of Cutaneous Leishmaniasis. PLoS One 16(1):e0243978","DOI":"10.1371\/journal.pone.0243978"},{"issue":"10","key":"8503_CR72","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0257043","volume":"16","author":"GB Tonelli","year":"2021","unstructured":"Tonelli GB, Binder C, Nogueira VLC, Prado MH, Theobaldo GG, Campos AM, Souza CM, Andrade Filho JD (2021) The sand fly (Diptera: Psychodidae) fauna of the urban area of Lassance, Northeast Minas Gerais. Brazil Plos One 16(10):e0257043. https:\/\/doi.org\/10.1371\/journal.pone.0257043","journal-title":"Brazil Plos One"},{"issue":"5","key":"8503_CR73","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002205","volume":"7","author":"SO Toz","year":"2013","unstructured":"Toz SO, Culha G, Zeyrek FY, Ertabaklar H, Alkan MZ, Vardarli AT, Gunduz C, Ozbel Y (2013) A Real-time ITS1-PCR based method in the diagnosis and species identification of Leishmania parasite from human and dog clinical samples in Turkey. PLoS Negl Trop Dis 7(5):e2205. https:\/\/doi.org\/10.1371\/journal.pntd.0002205","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"8503_CR74","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.03.010","volume":"12","author":"BL Travi","year":"2018","unstructured":"Travi BL, Cordeiro-da-Silva A, Dantas-Torres F, Mir\u00f3 G (2018) Canine Visceral Leishmaniasis: Diagnosis and management of the reservoir living among us. PLoS Negl Trop Dis 12(1):e0006082. https:\/\/doi.org\/10.1016\/j.vetpar.2013.03.010","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"8503_CR75","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001956","volume":"7","author":"P Tsukayama","year":"2013","unstructured":"Tsukayama P, N\u00fa\u00f1ez JH, De Los SM, Sober\u00f3n V, Lucas CM, Matlashewski G, Llanos-Cuentas A, Ore M, Baldeviano GC, Edgel KA, Lescano AG, Graf PCF, Bacon DJ (2013) A FRET-Based Real-Time PCR assay to identify the main causal agents of New World Tegumentary Leishmaniasis. PLoS Negl Trop Dis 7(1):e1956. https:\/\/doi.org\/10.1371\/journal.pntd.0001956","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"8503_CR76","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1111\/tmi.12047","volume":"18","author":"S Verma","year":"2013","unstructured":"Verma S, Bhandari V, Avishek K, Ramesh V, Salotra P (2013) Reliable diagnosis of Post-Kala-Azar Dermal Leishmaniasis (PKDL) using slit aspirate specimen to avoid invasive sampling procedures. Tropical Med Int Health 18(3):268\u201375. https:\/\/doi.org\/10.1111\/tmi.12047","journal-title":"Tropical Med Int Health"},{"issue":"4","key":"8503_CR77","doi-asserted-by":"publisher","first-page":"1607","DOI":"10.1007\/s00436-012-2999-2","volume":"111","author":"MA Viol","year":"2012","unstructured":"Viol MA, Lima VMF, Aquino MCC, Gallo G, Alves IP, Generoso D, Perri SHV, Lucheis SB, Langoni H, Nunes CM, Bresciani KDS (2012) Detection of cross infections by Leishmania spp. and Trypanosoma spp. in dogs using Indirect Immunoenzyme Assay, Indirect Fluorescent Antibody Test and Polymerase Chain Reaction. Parasitol Res 111(4):1607\u20131613. https:\/\/doi.org\/10.1007\/s00436-012-2999-2","journal-title":"Parasitol Res"},{"key":"8503_CR78","unstructured":"World Health Organization (2021) Global leishmaniasis surveillance: 2021, assessing the impact of the COVID-19 pandemic. https:\/\/www.who.int\/publications\/i\/item\/who-wer9745-575-590. Accessed 26 April 2023"},{"key":"8503_CR79","doi-asserted-by":"crossref","unstructured":"Wortmann G, Hochberg L, Houng HH, Sweeney C, Zapor M, Aronson N, Weina P, Ockenhouse CF (2005) Rapid identification of Leishmania complexes by a real-time PCR assay. Am J Trop Med Hyg, 73(6): 999\u20131004. https:\/\/pubmed.ncbi.nlm.nih.gov\/16354801\/.","DOI":"10.4269\/ajtmh.2005.73.999"},{"key":"8503_CR80","doi-asserted-by":"publisher","first-page":"1406632","DOI":"10.3389\/fmicb.2024.1406632","volume":"15","author":"N Yang","year":"2024","unstructured":"Yang N, Zhang H, Han X, Liu Z, Lu Y (2024) Advancements and applications of loop-mediated isothermal amplification technology: a comprehensive overview. Front Microbiol 15:1406632. https:\/\/doi.org\/10.3389\/fmicb.2024.1406632","journal-title":"Front Microbiol"},{"issue":"1","key":"8503_CR81","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-016-1721-0","volume":"9","author":"EE Zijilstra","year":"2016","unstructured":"Zijilstra EE (2016) The immunology of post-kala-azar dermal leishmaniasis (PKDL). Parasit Vectors 9(1):1\u20139. https:\/\/doi.org\/10.1186\/s13071-016-1721-0","journal-title":"Parasit Vectors"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-025-08503-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00436-025-08503-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-025-08503-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T11:02:30Z","timestamp":1748948550000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00436-025-08503-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":81,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["8503"],"URL":"https:\/\/doi.org\/10.1007\/s00436-025-08503-2","relation":{},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2 June 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 May 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 May 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"54"}}