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N\u00b05962\/22 and 872\/23"]}],"id":[{"id":"10.13039\/501100003947","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Bern"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Domestic goats (<jats:italic>Capra aegagrus hircus<\/jats:italic>) are intermediate hosts for three species of <jats:italic>Sarcocystis<\/jats:italic>: <jats:italic>S. capracanis<\/jats:italic>, <jats:italic>S. hircicanis<\/jats:italic>, and <jats:italic>S. moulei<\/jats:italic>. The present study aimed to describe and identify <jats:italic>Sarcocystis<\/jats:italic> spp. in goat\/kid muscles utilizing optical and transmission electron microscopy, a serological method, and PCR-sequencing of individual cysts. Both <jats:italic>S. capracanis<\/jats:italic> and <jats:italic>S. hircicanis<\/jats:italic> were identified by light and transmission electron microscopy and molecular methods. No macroscopic cysts were detected in any of the examined animals (<jats:italic>n<\/jats:italic>\u2009=\u200990, from two Argentinean provinces); only microscopic sarcocysts of <jats:italic>Sarcocystis<\/jats:italic> spp. were detected in 17 of 19 adult goats (90%), but in no kids (<jats:italic>n<\/jats:italic>\u2009=\u200971) aged 37 to 290\u00a0days. Fourteen <jats:italic>18S rRNA<\/jats:italic> and the six <jats:italic>coxI<\/jats:italic> sequences from individual cysts highlighted high similarity (99.06\u2013100% and 98.89\u201399.81%, respectively) with <jats:italic>S. capracanis<\/jats:italic> sequences from other regions. Two distinct groups of <jats:italic>S. capracanis 18S rRNA<\/jats:italic> sequences with high intra-group identities (99.42\u2013100%) were identified, as well as another sequence that did not group. Each group was integrated from samples of both sampled provinces. Despite relatively extensive differences between these groups of sequences (up to 3.8%), the phylogenetic analyses positioned the three representative sequences all together in a branch with <jats:italic>S. capracanis<\/jats:italic> sequences but also containing <jats:italic>S. tenella<\/jats:italic>. In addition, one <jats:italic>18S rRNA<\/jats:italic> sequence showed a 100% identity with those of <jats:italic>S. hircicanis<\/jats:italic>. Two animals harbor <jats:italic>S. capracanis<\/jats:italic> and <jats:italic>S. hircicanis<\/jats:italic> cysts. Twelve out of 18 adult goats were seropositive by <jats:italic>Sarcocystis<\/jats:italic> spp. IFAT with titers\u2009\u2264\u2009100. This is the first report that confirmed the infection and co-infection of <jats:italic>S. capracanis<\/jats:italic> and <jats:italic>S. hircicanis<\/jats:italic> in domestic goats from Argentina and evidenced a high variability in the <jats:italic>18S rRNA<\/jats:italic> fragment sequences of <jats:italic>S. capracanis<\/jats:italic>.<\/jats:p>","DOI":"10.1007\/s00436-025-08519-8","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T06:56:03Z","timestamp":1751007363000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identifying Sarcocystis spp. infection in goats: a combined morphological, serological, and molecular approach"],"prefix":"10.1007","volume":"124","author":[{"given":"K. D.","family":"Steffen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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O.","family":"Arias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Mor\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"key":"8519_CR1","doi-asserted-by":"publisher","unstructured":"Bittencourt MV, Meneses IDS, Ribeiro-Andrade M, de Jesus RF, de Ara\u00fajo FR, Gondim LFP (2016) Sarcocystis spp. in sheep and goats: frequency of infection and species identification by morphological, ultrastructural, and molecular tests in Bahia, Brazil. Parasitol Res 115(4):1683\u20131689. https:\/\/doi.org\/10.1007\/S00436-016-4909-5","DOI":"10.1007\/S00436-016-4909-5"},{"key":"8519_CR2","unstructured":"Buxton D (1998) Protozoan infections (Toxoplasma gondii, Neospora caninum and Sarcocystis spp.) in sheep and goats: recent advances. 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Furthermore, we confirmed that no generative artificial intelligence (AI) and AI-assisted technologies were used in the writing process.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"We confirm that the manuscript has been read and approved by all named\u00a0authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors\u00a0listed in the manuscript has been approved by all of us.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"All authors agree with this submission.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"71"}}