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Felines, the definitive hosts of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    , play a crucial role in the transmission of toxoplasmosis. The booming pet industry has led to more cats and cat-owning families, increasing the risk of toxoplasmosis transmission from animals to humans. Monitoring feline\n                    <jats:italic>T. gondii<\/jats:italic>\n                    infection accurately is crucial for reducing transmission risks. However, existing diagnostic methods focus on detecting whether cats are infected with\n                    <jats:italic>T. gondii<\/jats:italic>\n                    but fail to trace whether feline toxoplasmosis infections originate from oocysts or cysts. In this study, we assessed four late-stage development abundant proteins that were highly expressed specifically in sporulated oocysts to evaluate their specificity in binding to cat anti-\n                    <jats:italic>T. gondii<\/jats:italic>\n                    -oocyst serum. The LEA880 protein can only specifically react with cat anti-\n                    <jats:italic>T. gondii<\/jats:italic>\n                    -oocyst positive serum, but not with cat anti-\n                    <jats:italic>T. gondii<\/jats:italic>\n                    -cyst positive serum or negative cat serum. The optimized indirect enzyme-linked immunosorbent assay (iELISA) method based on the LEA880 protein exhibits good specificity and sensitivity in detecting\n                    <jats:italic>T. gondii<\/jats:italic>\n                    oocyst infection in cats.\n                  <\/jats:p>","DOI":"10.1007\/s00436-025-08523-y","type":"journal-article","created":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T20:01:02Z","timestamp":1751832062000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development and optimization of an ELISA method to detect Toxoplasma gondii oocyst infection in cats"],"prefix":"10.1007","volume":"124","author":[{"given":"Mingfeng","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bufan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengming","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulian","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bang","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"8523_CR1","doi-asserted-by":"publisher","first-page":"e02868","DOI":"10.1128\/mbio.02868-22","volume":"14","author":"D Arranz-Sol\u00eds","year":"2023","unstructured":"Arranz-Sol\u00eds D, Warschkau D, Fabian BT, Seeber F, Saeij JPJ (2023) Late embryogenesis abundant proteins contribute to the resistance of Toxoplasma gondii oocysts against environmental stresses. 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All the mice were sacrificed after the experiment. Our study was authorized by the animal ethical committee from Huazhong Agricultural University in China (permit no. HZAUMO-2025\u20130123). Cats used for experimental purposes were purchased from a cat breeding institution in Hubei Province of China. All cat serum samples were collected from the Animal Hospital of Huazhong Agricultural University.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"All authors gave their consent before submitting this work.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"79"}}