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Whereas several anticoagulants, including two putative hirudins of\n                    <jats:italic>A. fenestrata<\/jats:italic>\n                    , have already been identified in previous investigations, functional characterizations of these components are yet missing. We conducted an in-depth re-analysis of the salivary gland transcriptomic data set of\n                    <jats:italic>A. fenestrata<\/jats:italic>\n                    and identified two additional hirudin-like factors that exhibit a remarkable structural feature\u2014the presence of an RGD motif at or near the N-terminal end of the molecules. The presence of such an RGD motif is a key feature of leech-derived platelet aggregation inhibitors such as ornatins and decorsins. However, both in ornatins and in decorsins, the respective RGD motif is located within the molecule between the 5th and the 6th cysteine residues, but not at or near the N-terminus. We named the two putative platelet aggregation inhibitors \u201cfenestrins.\u201d Both the two putative hirudins and the two fenestrins of\n                    <jats:italic>A. fenestrata<\/jats:italic>\n                    were expressed as recombinant proteins, purified, and functionally characterized in either coagulation or platelet aggregation assays.\n                  <\/jats:p>","DOI":"10.1007\/s00436-025-08578-x","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T02:28:40Z","timestamp":1762482520000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hirudins and fenestrins of the African medicinal leech Asiaticobdella fenestrata"],"prefix":"10.1007","volume":"124","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1479-7945","authenticated-orcid":false,"given":"Lucia","family":"Schulz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0925-6745","authenticated-orcid":false,"given":"C\u00e9line","family":"Tolksdorf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6003-4662","authenticated-orcid":false,"given":"Bernhard H.","family":"Rauch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3200-6207","authenticated-orcid":false,"given":"Sebastian","family":"Kvist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1929-1728","authenticated-orcid":false,"given":"Christian","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,7]]},"reference":[{"issue":"12","key":"8578_CR1","doi-asserted-by":"publisher","first-page":"e4825","DOI":"10.1002\/pro.4825","volume":"32","author":"L Acquasaliente","year":"2023","unstructured":"Acquasaliente L, Pierangelini A, Pagotto A, Pozzi N, De Filippis V (2023) From haemadin to haemanorm: synthesis and characterization of full-length haemadin from the leech Haemadipsa sylvestris and of a novel bivalent, highly potent thrombin inhibitor (haemanorm). 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