{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:01:19Z","timestamp":1777982479465,"version":"3.51.4"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Research Project of Tibet Autonomous Region"},{"name":"Henan Province modern agricultural industrial technology system"},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasitol Res"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s00436-026-08663-9","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:30:16Z","timestamp":1773815416000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First identification of Sarcocystis medusiformis in Chinese sheep (Ovis aries): morphological and molecular characterization"],"prefix":"10.1007","volume":"125","author":[{"given":"Danqu","family":"Lamu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luyao","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhun","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangsheng","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Tao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yurong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjie","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"issue":"2","key":"8663_CR1","doi-asserted-by":"publisher","first-page":"262","DOI":"10.2307\/3284453","volume":"83","author":"JR Barta","year":"1997","unstructured":"Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW, Feighner SD, Elbrecht A, Perkins-Barrow A, Jenkins MC, Danforth HD, Ruff MD, Profous-Juchelka H (1997) Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 83(2):262\u2013271","journal-title":"J Parasitol"},{"issue":"10","key":"8663_CR2","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1080\/00480169.1979.34651","volume":"27","author":"GH Collins","year":"1979","unstructured":"Collins GH, Atkinson E, Charleston WA (1979) Studies on Sarcocystis species III: The macrocystic species of sheep. N Z Vet J 27(10):204\u2013206. https:\/\/doi.org\/10.1080\/00480169.1979.34651","journal-title":"N Z Vet J"},{"issue":"Suppl2","key":"8663_CR3","doi-asserted-by":"publisher","first-page":"W13","DOI":"10.1093\/nar\/gkr245","volume":"39","author":"P Di Tommaso","year":"2011","unstructured":"Di Tommaso P, Moretti S, Xenarios I, Orobitg M, Montanyola A, Chang JM, Taly JF, Notredame C (2011) T-Coffee: A web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension. Nucl Acids Res 39(Suppl2):W13\u2013W17. https:\/\/doi.org\/10.1093\/nar\/gkr245","journal-title":"Nucl Acids Res"},{"issue":"1","key":"8663_CR4","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1186\/s12917-018-1712-9","volume":"14","author":"H Dong","year":"2018","unstructured":"Dong H, Su R, Wang Y, Tong Z, Zhang L, Yang Y, Hu J (2018) Sarcocystis species in wild and domestic sheep (Ovis ammon and Ovis aries) from China. BMC Vet Res 14(1):377. https:\/\/doi.org\/10.1186\/s12917-018-1712-9","journal-title":"BMC Vet Res"},{"key":"8663_CR5","volume-title":"Sarcocystosis of animals and humans","author":"JP Dubey","year":"2016","unstructured":"Dubey JP, Calero-Bernal R, Rosenthal BM, Speer CA, Fayer R (2016) Sarcocystosis of animals and humans, 2nd edn. CRC, Boca Raton, FL, USA","edition":"2"},{"issue":"2","key":"8663_CR6","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1007\/s00436-020-07002-w","volume":"120","author":"A El-Morsey","year":"2021","unstructured":"El-Morsey A, Abdo W, Zaid AAA, Sorour SSG (2021) Morphologic and molecular identification of three macroscopic Sarcocystis species infecting domestic sheep (Ovis aries) and cattle (Bos taurus) in Egypt. Parasitol Res 120(2):637\u2013654. https:\/\/doi.org\/10.1007\/s00436-020-07002-w","journal-title":"Parasitol Res"},{"issue":"3","key":"8663_CR7","first-page":"181","volume":"5","author":"F Farhang-Pajuh","year":"2014","unstructured":"Farhang-Pajuh F, Yakhchali M, Mardani K (2014) Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran. Vet Res Forum 5(3):181\u2013186","journal-title":"Vet Res Forum"},{"issue":"7","key":"8663_CR8","doi-asserted-by":"publisher","first-page":"902","DOI":"10.3390\/pathogens12070902","volume":"12","author":"Y Feng","year":"2023","unstructured":"Feng Y, Guo R, Sang X, Zhang X, Li M, Li X, Yang N, Jiang T (2023) A systematic meta-analysis of global Sarcocystis infection in sheep and goats. Pathogens 12(7):902. https:\/\/doi.org\/10.3390\/pathogens12070902","journal-title":"Pathogens"},{"issue":"6","key":"8663_CR9","doi-asserted-by":"publisher","first-page":"916","DOI":"10.2307\/3284040","volume":"81","author":"CK Fenger","year":"1995","unstructured":"Fenger CK, Granstrom DE, Langemeier JL, Stamper S, Donahue JM, Patterson JS, Gajadhar AA, Marteniuk JV, Xiaomin Z, Dubey JP (1995) Identification of opossums (Didelphis virginiana) as the putative definitive host of Sarcocystis neurona. J Parasitol 81(6):916\u2013919","journal-title":"J Parasitol"},{"key":"8663_CR10","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1016\/j.ijpara.2013.02.004","volume":"43","author":"B Gjerde","year":"2013","unstructured":"Gjerde B (2013) Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. Int J Parasitol 43:579\u2013591. https:\/\/doi.org\/10.1016\/j.ijpara.2013.02.004","journal-title":"Int J Parasitol"},{"key":"8663_CR11","doi-asserted-by":"publisher","first-page":"3501","DOI":"10.1007\/s00436-014-4062-y","volume":"113","author":"B Gjerde","year":"2014","unstructured":"Gjerde B (2014a) Molecular characterisation of Sarcocystis rileyi from a common eider (Somateria mollissima) in Norway. Parasitol Res 113:3501\u20133509. https:\/\/doi.org\/10.1007\/s00436-014-4062-y","journal-title":"Parasitol Res"},{"key":"8663_CR12","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1017\/S0031182013001819","volume":"141","author":"B Gjerde","year":"2014","unstructured":"Gjerde B (2014b) Sarcocystis species in red deer revisited: with a re-description of two known species as Sarcocystis elongata n. sp. and Sarcocystis truncata n. Sp. based on mitochondrial cox1 sequences. Parasitol 141:441\u2013452. https:\/\/doi.org\/10.1017\/S0031182013001819","journal-title":"Parasitol"},{"key":"8663_CR13","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1007\/s00436-019-06504-6","volume":"119","author":"B Gjerde","year":"2020","unstructured":"Gjerde B, de la Fuente C, Alunda JM, Luz\u00f3n M (2020) Molecular characterisation of five Sarcocystis species in domestic sheep (Ovis aries) from Spain. Parasitol Res 119:215\u2013231. https:\/\/doi.org\/10.1007\/s00436-019-06504-6","journal-title":"Parasitol Res"},{"key":"8663_CR14","first-page":"95","volume":"41","author":"TA Hall","year":"1999","unstructured":"Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT. Nucl Acids Symp Ser 41:95\u201398","journal-title":"Nucl Acids Symp Ser"},{"key":"8663_CR15","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1051\/parasite\/2017025","volume":"24","author":"JJ Hu","year":"2017","unstructured":"Hu JJ, Huang S, Wen T, Esch GW, Liang Y, Li HL (2017) Sarcocystis spp. in domestic sheep in Kunming City, China: Prevalence, morphology, and molecular characteristics. Parasite 24:30. https:\/\/doi.org\/10.1051\/parasite\/2017025","journal-title":"Parasite"},{"issue":"10","key":"8663_CR16","doi-asserted-by":"publisher","first-page":"504","DOI":"10.3390\/vetsci11100504","volume":"11","author":"Y Kang","year":"2024","unstructured":"Kang Y, Lu XS, He YH, Wang C, Wu ZX, Wang L, Wu XJ, Hu JJ, Zhu XQ (2024) First molecular identification and prevalence of Sarcocystis spp. in sheep intended for human consumption in Shanxi province, China. Vet Sci 11(10):504. https:\/\/doi.org\/10.3390\/vetsci11100504","journal-title":"Vet Sci"},{"issue":"4","key":"8663_CR17","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1007\/BF00348582","volume":"41","author":"H Mehlhorn","year":"1973","unstructured":"Mehlhorn H, Scholtyseck E (1973) The fine structure of the cyst stages of Sarcocystis tenella. Z Parasitenkd 41(4):291\u2013310","journal-title":"Z Parasitenkd"},{"issue":"6","key":"8663_CR18","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1016\/s0020-7519(99)00062-4","volume":"29","author":"NB Mugridge","year":"1999","unstructured":"Mugridge NB, Morrison DA, Johnson AM, Luton K, Dubey JP, Vot\u00fdpka J, Tenter AM (1999) Phylogenetic relationships of the genus Frenkelia: a review of its history and new knowledge gained from comparison of large subunit ribosomal ribonucleic acid gene sequences. Int J Parasitol 29(6):957\u2013972. https:\/\/doi.org\/10.1016\/s0020-7519(99)00062-4","journal-title":"Int J Parasitol"},{"key":"8663_CR19","doi-asserted-by":"publisher","first-page":"1225796","DOI":"10.3389\/fvets.2023.1225796","volume":"10","author":"S Nawshirwan","year":"2023","unstructured":"Nawshirwan S, Heucken N, Piekarek N, van Beers T, Fulgham-Scott N, Grandoch A, Neiss WF, Vogt J, Barham M (2023) Morphological, ultrastructural, genetic characteristics and remarkably low prevalence of macroscopic Sarcocystis species isolated from sheep and goats in Kurdistan region, Iraq. Front Vet Sci 10:1225796. https:\/\/doi.org\/10.3389\/fvets.2023.1225796","journal-title":"Front Vet Sci"},{"issue":"9","key":"8663_CR21","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1111\/j.1751-0813.1986.tb08065.x","volume":"63","author":"PJ O\u2019Donoghue","year":"1986","unstructured":"O\u2019Donoghue PJ, Ford GE (1986) The prevalence and intensity of Sarcocystis spp. infections in sheep. Aust Vet J 63(9):273\u2013278. https:\/\/doi.org\/10.1111\/j.1751-0813.1986.tb08065.x","journal-title":"Aust Vet J"},{"issue":"1\u20132","key":"8663_CR20","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/0304-4017(87)90130-0","volume":"24","author":"DL Obendorf","year":"1987","unstructured":"Obendorf DL, Munday BL (1987) Experimental infection with Sarcocystis medusiformis in sheep. Vet Parasitol 24(1\u20132):59\u201365. https:\/\/doi.org\/10.1016\/0304-4017(87)90130-0","journal-title":"Vet Parasitol"},{"issue":"3","key":"8663_CR22","doi-asserted-by":"publisher","first-page":"103","DOI":"10.3390\/vetsci11030103","volume":"11","author":"MP Peris","year":"2024","unstructured":"Peris MP, Gracia MJ, Moreno B, Juan-Puente P, Morales M, Serrano M, Manzano MD, Halaihel N, Badiola J, Castillo JA (2024) Identification of Sarcocystis spp. in slaughtered sheep from Spain and evaluation of bradyzoite viability after freezing. Vet Sci 11(3):103. https:\/\/doi.org\/10.3390\/vetsci11030103","journal-title":"Vet Sci"},{"key":"8663_CR23","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.vprsr.2016.05.004","volume":"3\u20134","author":"AP Pipia","year":"2016","unstructured":"Pipia AP, Varcasia A, Zidda A, Dess\u00ec G, Panzalis R, Tamponi C, Marrosu R, Tosciri G, Sanna G, Dore F, Chiesa F, Scala A (2016) Cross-sectional investigation on sheep sarcosporidiosis in Sardinia, Italy. Vet Parasitol Reg Stud Rep 3\u20134:13\u201317. https:\/\/doi.org\/10.1016\/j.vprsr.2016.05.004","journal-title":"Vet Parasitol Reg Stud Rep"},{"key":"8663_CR24","first-page":"130","volume":"40","author":"A Railliet","year":"1886","unstructured":"Railliet A (1886) Psorospermies geanles dans L\u2019oesophage et les muscles du mouton. Bull Acad V\u00e9t Fr 40:130\u2013134","journal-title":"Bull Acad V\u00e9t Fr"},{"issue":"3","key":"8663_CR25","first-page":"565","volume":"38","author":"M Stolte","year":"1996","unstructured":"Stolte M, Odening K, Bockhardt I (1996) Antelopes (Bovidae) kept in European zoological gardens as intermediate hosts of Sarcocystis species. Parassitologia 38(3):565\u2013570","journal-title":"Parassitologia"},{"key":"8663_CR26","doi-asserted-by":"publisher","first-page":"102219","DOI":"10.1016\/j.parint.2020.102219","volume":"80","author":"Y Sun","year":"2021","unstructured":"Sun Y, Ju J, Su X, Xie C, Li Y, Kang M (2021) Infection survey and morphological characteristics of Sarcocystis spp. in naturally infected Tibetan sheep from Qinghai in northwestern China. Parasitol Int 80:102219. https:\/\/doi.org\/10.1016\/j.parint.2020.102219","journal-title":"Parasitol Int"},{"issue":"7","key":"8663_CR27","doi-asserted-by":"publisher","first-page":"3022","DOI":"10.1093\/molbev\/msab120","volume":"38","author":"K Tamura","year":"2021","unstructured":"Tamura K, Stecher G, Kumar S (2021) MEGA11: Molecular evolutionary genetics analysis version 11. Mol Biol Evol 38(7):3022\u20133027. https:\/\/doi.org\/10.1093\/molbev\/msab120","journal-title":"Mol Biol Evol"},{"key":"8663_CR28","first-page":"37","volume":"1","author":"G Wang","year":"1989","unstructured":"Wang G, Wei T, Wang X et al (1989) Sarcocystis cystiformis n. sp. infecting sheep. Xinjiang Agric Sci 1:37\u201340 (In Chinese)","journal-title":"Xinjiang Agric Sci"}],"container-title":["Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00436-026-08663-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-026-08663-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00436-026-08663-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:33:44Z","timestamp":1773815624000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00436-026-08663-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,18]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["8663"],"URL":"https:\/\/doi.org\/10.1007\/s00436-026-08663-9","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-8423378\/v1","asserted-by":"object"}]},"ISSN":["0932-0113","1432-1955"],"issn-type":[{"value":"0932-0113","type":"print"},{"value":"1432-1955","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,18]]},"assertion":[{"value":"22 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 March 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 March 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The animal study protocol was approved by the Animal Ethics Committee of Yunnan University (permission number YNU20230466, received date: 3 March 2023).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to publish"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"45"}}