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Amphistomes were collected from 158 cattle originating from 14 localities, slaughtered in abattoirs in Harare and Bulawayo. One-hundred and twenty-one amphistome specimens were selected and sectioned into two portions, one piece for morphological characterization based on histological sectioning using haematoxylin and eosin staining, and the other piece for molecular identification based on the ITS-2 gene. Morphological characters classified the amphistomes into ten species as follows;\n                    <jats:italic>Calicophoron (C.) calicophorum<\/jats:italic>\n                    ,\n                    <jats:italic>C. microbothrium<\/jats:italic>\n                    ,\n                    <jats:italic>C. clavula<\/jats:italic>\n                    ,\n                    <jats:italic>C. raja<\/jats:italic>\n                    ,\n                    <jats:italic>C. phillerouxi<\/jats:italic>\n                    ,\n                    <jats:italic>C. sukari<\/jats:italic>\n                    ,\n                    <jats:italic>Paramphistomum (P.) gracile<\/jats:italic>\n                    ,\n                    <jats:italic>P. hibarniae<\/jats:italic>\n                    ,\n                    <jats:italic>Carmyerius (Ca.) multivitellarius<\/jats:italic>\n                    ,\n                    <jats:italic>Cotylophoron (Co.) cotylophorum<\/jats:italic>\n                    , and unidentified\n                    <jats:italic>Paramphistomum<\/jats:italic>\n                    sp.\n                    <jats:italic>Calicophoron<\/jats:italic>\n                    was the most common genus, and\n                    <jats:italic>C. microbothrium<\/jats:italic>\n                    was the most common and widely distributed species recorded across the\u00a014 localities. The ITS-2 gene was able to confirm the identification of\n                    <jats:italic>C. microbothrium<\/jats:italic>\n                    ,\n                    <jats:italic>C. clavula<\/jats:italic>\n                    ,\n                    <jats:italic>C. raja<\/jats:italic>\n                    ,\n                    <jats:italic>C. calicophorum<\/jats:italic>\n                    and\n                    <jats:italic>C. phillerouxi<\/jats:italic>\n                    . There were no comparative ITS-2 sequences for\n                    <jats:italic>C. sukari<\/jats:italic>\n                    ,\n                    <jats:italic>P. gracile<\/jats:italic>\n                    and\n                    <jats:italic>P. hibarniae<\/jats:italic>\n                    on the GenBank. Hence, specimens identified morphologically as\n                    <jats:italic>C. sukari<\/jats:italic>\n                    showed a\u00a0100% homology to multiple\n                    <jats:italic>Calicophoron<\/jats:italic>\n                    species, but grouped with\n                    <jats:italic>C. raja.<\/jats:italic>\n                    Furthermore,\n                    <jats:italic>Ca. multivitellarius<\/jats:italic>\n                    specimens confirmed the genus identity by forming sister clade with other\n                    <jats:italic>Carmyerius<\/jats:italic>\n                    spp. There were no species-level resolution for\n                    <jats:italic>Paramphistomum<\/jats:italic>\n                    sp.,\n                    <jats:italic>P. gracile<\/jats:italic>\n                    and\n                    <jats:italic>P. hibarniae<\/jats:italic>\n                    , as these isolates formed a clade with\n                    <jats:italic>Paramphistomum<\/jats:italic>\n                    sp. (OQ459353.1). The study resulted in new geographical records for\n                    <jats:italic>P. gracile<\/jats:italic>\n                    ,\n                    <jats:italic>P. hibarniae<\/jats:italic>\n                    and\n                    <jats:italic>Ca. multivitellarius<\/jats:italic>\n                    in Zimbabwe, hence adding new knowledge to the geographical distribution of these species within Africa.\n                  <\/jats:p>","DOI":"10.1007\/s00436-026-08682-6","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T18:04:57Z","timestamp":1778177097000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Morphological and molecular identification of amphistomes occurring in cattle in Zimbabwe"],"prefix":"10.1007","volume":"125","author":[{"given":"Ignore","family":"Nyagura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mokgadi P.","family":"Malatji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline S.","family":"Sibula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philile I.","family":"Ngcamphalala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samson","family":"Mukaratirwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,7]]},"reference":[{"key":"8682_CR1","doi-asserted-by":"publisher","first-page":"2511","DOI":"10.1007\/s00436-020-06749-6","volume":"119","author":"PV Alves","year":"2020","unstructured":"Alves PV, Assis JC, L\u00f3pez-Hern\u00e1ndez D et al (2020) A phylogenetic study of the cecal amphistome Zygocotyle lunata (Trematoda: Zygocotylidae), with notes on the molecular systematics of Paramphistomoidea. 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