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High levels of benzimidazole-resistant\n                    <jats:italic>Trichostrongylus<\/jats:italic>\n                    populations have been reported in Thailand, where the resistant allele frequency of\n                    <jats:italic>Trichostrongylus<\/jats:italic>\n                    populations were consistently higher than the resistant allele frequency of\n                    <jats:italic>Haemonchus.<\/jats:italic>\n                    Despite its zoonotic importance and the widespread use of benzimidazoles for gastrointestinal nematode infections in livestock,\n                    <jats:italic>Trichostrongylus<\/jats:italic>\n                    is often overshadowed by the more pathogenic\n                    <jats:italic>Haemonchus<\/jats:italic>\n                    . Here, we aim to assess the benzimidazole resistance status of\n                    <jats:italic>Trichostrongylus colubriformis<\/jats:italic>\n                    adults in Thailand, and investigate their genetic diversity and population structure. Using 60\n                    <jats:italic>T. colubriformis<\/jats:italic>\n                    adults from Sukhothai and Satun, benzimidazole resistance was genotypically detected using the\n                    <jats:italic>b-tubulin<\/jats:italic>\n                    gene. Genetic diversity was investigated by amplification of the mitochondrial\n                    <jats:italic>COI<\/jats:italic>\n                    gene of representative specimens. Significant levels of benzimidazole resistance was detected, with the heterozygous (RS) genotype as the majority (81.7%). The population genetic structure revealed high genetic diversity of\n                    <jats:italic>T. colubriformis<\/jats:italic>\n                    populations and high genetic differentiation between the populations from Thailand and other countries, indicative of limited genetic exchange between populations. Despite significant levels of benzimidazole resistance in Thailand\u2019s\n                    <jats:italic>T. colubriformis<\/jats:italic>\n                    population, the population structure, where limited genetic exchange and high genetic differentiation was observed between populations, suggests that locally targeted measures could be effective to curb anthelminthic resistance spread. However, additionally surveillance of this zoonotic nematode is advocated to sustainably safeguard animal and human health.\n                  <\/jats:p>","DOI":"10.1007\/s00436-026-08713-2","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:06:24Z","timestamp":1781532384000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genotypic evidence of benzimidazole resistance and population structure insights in zoonotic Trichostrongylus colubriformis from Thailand"],"prefix":"10.1007","volume":"125","author":[{"given":"Abigail Hui En","family":"Chan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wallop","family":"Pakdee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanisara","family":"Kaenkaew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Punyanuch","family":"Kaewwongwattana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sivapong","family":"Sungpradit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urusa","family":"Thaenkham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"8713_CR1","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.ijpara.2010.07.014","volume":"41","author":"E Archie","year":"2011","unstructured":"Archie E, Ezenwa V (2011) Population genetic structure and history of a generalist parasite infecting multiple sympatric host species. 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