{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T12:14:30Z","timestamp":1784031270870,"version":"3.55.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_200354"],"award-info":[{"award-number":["310030_200354"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Genet Genomics"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Investigations of hereditary phenotypes in spontaneous mutants may help to better understand the physiological functions of the altered genes. We investigated two unrelated domestic shorthair cats with bulbous swellings of the hair shafts. The clinical, histopathological, and ultrastructural features were similar to those in mice with lanceolate hair phenotype caused by loss-of-function variants in <jats:italic>Dsg4<\/jats:italic> encoding desmoglein 4. We sequenced the genomes from both affected cats and compared the data of each affected cat to 61 control genomes. A search for private homozygous variants in the <jats:italic>DSG4<\/jats:italic> candidate gene revealed independent frameshift variants in each case, c.76del or p.Ile26fsLeu*4 in case no. 1 and c.1777del or p.His593Thrfs*23 in case no. 2. DSG4 is a transmembrane glycoprotein located primarily in the extracellular part of desmosomes, a complex of adhesion molecules responsible for connecting the keratin intermediate filaments of neighbouring epithelial cells. Desmosomes are essential for normal hair shaft formation. Both identified <jats:italic>DSG4<\/jats:italic> variants in the affected cats lead to premature stop codons and truncate major parts of the open-reading frame. We assume that this leads to a complete loss of DSG4 function, resulting in an incorrect formation of the desmosomes and causing the development of defective hair shafts. Together with the knowledge on the effects of <jats:italic>DSG4<\/jats:italic> variants in other species, our data suggest that the identified <jats:italic>DSG4<\/jats:italic> variants cause the hair shaft dystrophy. To the best of our knowledge, this study represents the first report of pathogenic <jats:italic>DSG4<\/jats:italic> variants in domestic animals.<\/jats:p>","DOI":"10.1007\/s00438-021-01842-6","type":"journal-article","created":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T13:03:21Z","timestamp":1638968601000},"page":"147-154","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Independent DSG4 frameshift variants in cats with hair shaft dystrophy"],"prefix":"10.1007","volume":"297","author":[{"given":"Sarah","family":"Kiener","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1940-0341","authenticated-orcid":false,"given":"Ana","family":"Rostaher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7271-805X","authenticated-orcid":false,"given":"Silvia","family":"R\u00fcfenacht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8155-0041","authenticated-orcid":false,"given":"Vidhya","family":"Jagannathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1523-5430","authenticated-orcid":false,"given":"John P.","family":"Sundberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4876-5112","authenticated-orcid":false,"given":"Monika","family":"Welle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0553-4880","authenticated-orcid":false,"given":"Tosso","family":"Leeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,12,8]]},"reference":[{"key":"1842_CR1","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1007\/s13555-019-0313-2","volume":"9","author":"A Ahmed","year":"2019","unstructured":"Ahmed A, Almohanna H, Griggs J, Tosti A (2019) Genetic hair disorders: a review. Dermatol Ther (heidelb) 9:421\u2013448. https:\/\/doi.org\/10.1007\/s13555-019-0313-2","journal-title":"Dermatol Ther (heidelb)"},{"key":"1842_CR2","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1111\/j.1432-0436.2006.00061.x","volume":"74","author":"H Bazzi","year":"2006","unstructured":"Bazzi H, Getz A, Mahoney MG, Ishida-Yamamoto A, Langbein L, Wahl JK 3rd, Christiano AM (2006) Desmoglein 4 is expressed in highly differentiated keratinocytes and trichocytes in human epidermis and hair follicle. Differentiation 74:129\u2013140. https:\/\/doi.org\/10.1111\/j.1432-0436.2006.00061.x","journal-title":"Differentiation"},{"key":"1842_CR3","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1016\/j.diff.2009.06.004","volume":"78","author":"H Bazzi","year":"2009","unstructured":"Bazzi H, Demehri S, Potter CS, Barber AG, Awgulewitsch A, Kopan R, Christiano AM (2009) Desmoglein 4 is regulated by transcription factors implicated in hair shaft differentiation. Differentiation 78:292\u2013300. https:\/\/doi.org\/10.1016\/j.diff.2009.06.004","journal-title":"Differentiation"},{"key":"1842_CR4","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1038\/sj.jid.5701038","volume":"128","author":"J Chen","year":"2008","unstructured":"Chen J, Jaeger K, Den Z, Koch PJ, Sundberg JP, Roop DR (2008) Mice expressing a mutant Krt75 (K6hf) allele develop hair and nail defects resembling pachyonychia congenita. J Invest Dermatol 128:270\u2013279. https:\/\/doi.org\/10.1038\/sj.jid.5701038","journal-title":"J Invest Dermatol"},{"key":"1842_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jaad.2008.04.002","volume":"59","author":"AS Cheng","year":"2008","unstructured":"Cheng AS, Bayliss SJ (2008) The genetics of hair shaft disorders. J Am Acad Dermatol 59:1\u201322. https:\/\/doi.org\/10.1016\/j.jaad.2008.04.002","journal-title":"J Am Acad Dermatol"},{"key":"1842_CR6","doi-asserted-by":"publisher","first-page":"80","DOI":"10.4161\/fly.19695","volume":"6","author":"P Cingolani","year":"2012","unstructured":"Cingolani P, Platts A, le Wang L, Coon M, Nguyen T, Wang L, Land SJ, Lu X, Ruden DM (2012) A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly (austin) 6:80\u201392. https:\/\/doi.org\/10.4161\/fly.19695","journal-title":"Fly (austin)"},{"key":"1842_CR7","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1111\/age.12834","volume":"50","author":"V Jagannathan","year":"2019","unstructured":"Jagannathan V, Dr\u00f6gem\u00fcller C, Leeb T, Dog Biomedical Variant Database Consortium (DBVDC) (2019) A comprehensive biomedical variant catalogue based on whole genome sequences of 582 dogs and eight wolves. Anim Genet 50:695\u2013704. https:\/\/doi.org\/10.1111\/age.12834","journal-title":"Anim Genet"},{"key":"1842_CR8","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1016\/j.ygeno.2003.11.015","volume":"83","author":"CA Jahoda","year":"2004","unstructured":"Jahoda CA, Kljuic A, O\u2019Shaughnessy R, Crossley N, Whitehouse CJ, Robinson M, Reynolds AJ, Demarchez M, Porter RM, Shapiro L, Christiano AM (2004) The lanceolate hair rat phenotype results from a missense mutation in a calcium coordinating site of the desmoglein 4 gene. Genomics 83:747\u2013756. https:\/\/doi.org\/10.1016\/j.ygeno.2003.11.015","journal-title":"Genomics"},{"key":"1842_CR9","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/s00403-006-0671-3","volume":"298","author":"P John","year":"2006","unstructured":"John P, Tariq M, Arshad Rafiq M, Amin-Ud-Din M, Muhammad D, Waheed I, Ansar M, Ahmad W (2006) Recurrent intragenic deletion mutation in desmoglein 4 gene underlies autosomal recessive hypotrichosis in two Pakistani families of Balochi and Sindhi origins. Arch Dermatol Res 298:135\u2013137. https:\/\/doi.org\/10.1007\/s00403-006-0671-3","journal-title":"Arch Dermatol Res"},{"key":"1842_CR10","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/S0092-8674(03)00273-3","volume":"113","author":"A Kljuic","year":"2003","unstructured":"Kljuic A, Bazzi H, Sundberg JP, Martinez-Mir A, O\u2019Shaughnessy R, Mahoney MG, Levy M, Montagutelli X, Ahmad W, Aita VM, Gordon D, Uitto J, Whiting D, Ott J, Fischer S, Gilliam TC, Jahoda CA, Morris RJ, Panteleyev AA, Nguyen VT, Christiano AM (2003) Desmoglein 4 in hair follicle differentiation and epidermal adhesion: evidence from inherited hypotrichosis and acquired pemphigus vulgaris. Cell 113:249\u2013260. https:\/\/doi.org\/10.1016\/S0092-8674(03)00273-3","journal-title":"Cell"},{"key":"1842_CR11","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1111\/j.0022-202X.2005.23903.x","volume":"125","author":"AG Messenger","year":"2005","unstructured":"Messenger AG, Bazzi H, Parslew R, Shapiro L, Christiano AM (2005) A missense mutation in the cadherin interaction site of the desmoglein 4 gene underlies localized autosomal recessive hypotrichosis. J Invest Dermatol 125:1077\u20131079. https:\/\/doi.org\/10.1111\/j.0022-202X.2005.23903.x","journal-title":"J Invest Dermatol"},{"key":"1842_CR12","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1111\/1523-1747.ep12297438","volume":"107","author":"X Montagutelli","year":"1996","unstructured":"Montagutelli X, Hogan ME, Aubin G, Lalouette A, Gu\u00e9net JL, King LE, Sundberg JP (1996) Lanceolate hair (lah): a recessive mouse mutation with alopecia and abnormal hair. J Invest Dermatol 107:20\u201325. https:\/\/doi.org\/10.1111\/1523-1747.ep12297438","journal-title":"J Invest Dermatol"},{"key":"1842_CR13","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1111\/j.0022-202X.2004.23311.x","volume":"123","author":"C Moss","year":"2004","unstructured":"Moss C, Martinez-Mir A, Lam H, Tadin-Strapps M, Kljuic A, Christiano AM (2004) A recurrent intragenic deletion in the desmoglein 4 gene underlies localized autosomal recessive hypotrichosis. J Invest Dermatol 123:607\u2013610. https:\/\/doi.org\/10.1111\/j.0022-202X.2004.23311.x","journal-title":"J Invest Dermatol"},{"key":"1842_CR14","doi-asserted-by":"publisher","first-page":"1484","DOI":"10.1172\/JCI20480","volume":"114","author":"T Nagasaka","year":"2004","unstructured":"Nagasaka T, Nishifuji K, Ota T, Whittock NV, Amagai M (2004) Defining the pathogenic involvement of desmoglein 4 in pemphigus and staphylococcal scalded skin syndrome. J Clin Invest 114:1484\u20131492. https:\/\/doi.org\/10.1172\/JCI20480","journal-title":"J Clin Invest"},{"key":"1842_CR15","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1034\/j.1600-0625.2001.100601.x","volume":"10","author":"M Nakamura","year":"2001","unstructured":"Nakamura M, Sundberg JP, Paus R (2001) Mutant laboratory mice with abnormalities in hair follicle morphogenesis, cycling, and\/or structure: annotated tables. Exp Dermatol 10:369\u2013390. https:\/\/doi.org\/10.1034\/j.1600-0625.2001.100601.x","journal-title":"Exp Dermatol"},{"key":"1842_CR16","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.0022-202X.2004.22715.x","volume":"123","author":"MA Rafiq","year":"2004","unstructured":"Rafiq MA, Ansar M, Mahmood S, Haque S, Faiyaz-ul-Haque M, Leal SM, Ahmad W (2004) A recurrent intragenic deletion mutation in DSG4 gene in three Pakistani families with autosomal recessive hypotrichosis. J Invest Dermatol 123:247\u2013248. https:\/\/doi.org\/10.1111\/j.0022-202X.2004.22715.x","journal-title":"J Invest Dermatol"},{"key":"1842_CR17","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1111\/vde.12925","volume":"32","author":"A Rostaher","year":"2021","unstructured":"Rostaher A, Bettenay S, Specht L, Silva KA, Bechtold L, Chen J, Majzoub M, Mueller RS, Sundberg JP (2021) Hair follicle dystrophy in a litter of domestic cats resembling lanceolate hair mutant mice. Vet Dermatol 32:74-e14. https:\/\/doi.org\/10.1111\/vde.12925","journal-title":"Vet Dermatol"},{"key":"1842_CR18","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.1038\/sj.jid.5700237","volume":"126","author":"JV Schaffer","year":"2006","unstructured":"Schaffer JV, Bazzi H, Vitebsky A, Witkiewicz A, Kovich OI, Kamino H, Shapiro LS, Amin SP, Orlow SJ, Christiano AM (2006) Mutations in the desmoglein 4 gene underlie localized autosomal recessive hypotrichosis with monilethrix hairs and congenital scalp erosions. J Invest Dermatol 126:1286\u20131291. https:\/\/doi.org\/10.1038\/sj.jid.5700237","journal-title":"J Invest Dermatol"},{"key":"1842_CR19","doi-asserted-by":"publisher","first-page":"R132","DOI":"10.1016\/j.cub.2008.12.005","volume":"19","author":"MR Schneider","year":"2009","unstructured":"Schneider MR, Schmidt-Ullrich R, Paus R (2009) The hair follicle as a dynamic miniorgan. Curr Biol 19:R132-142. https:\/\/doi.org\/10.1016\/j.cub.2008.12.005","journal-title":"Curr Biol"},{"key":"1842_CR20","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1146\/annurev-genom-021610-131501","volume":"11","author":"Y Shimomura","year":"2010","unstructured":"Shimomura Y, Christiano AM (2010) Biology and genetics of hair. Annu Rev Genomics Hum Genet 11:109\u2013132. https:\/\/doi.org\/10.1146\/annurev-genom-021610-131501","journal-title":"Annu Rev Genomics Hum Genet"},{"key":"1842_CR21","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1038\/sj.jid.5700113","volume":"126","author":"Y Shimomura","year":"2006","unstructured":"Shimomura Y, Sakamoto F, Kariya N, Matsunaga K, Ito M (2006) Mutations in the desmoglein 4 gene are associated with monilethrix-like congenital hypotrichosis. J Invest Dermatol 126:1281\u20131285. https:\/\/doi.org\/10.1038\/sj.jid.5700113","journal-title":"J Invest Dermatol"},{"key":"1842_CR22","volume-title":"Handbook of mouse mutations with skin and hair abnormalities: animal models and biomedical tools","author":"JP Sundberg","year":"1994","unstructured":"Sundberg JP (1994) Handbook of mouse mutations with skin and hair abnormalities: animal models and biomedical tools. CRC Press, Boca Raton"},{"key":"1842_CR23","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1111\/1523-1747.ep12343152","volume":"106","author":"JP Sundberg","year":"1996","unstructured":"Sundberg JP, King LE (1996) Mouse mutations as animal models and biomedical tools for dermatological research. J Invest Dermatol 106:368\u2013376. https:\/\/doi.org\/10.1111\/1523-1747.ep12343152","journal-title":"J Invest Dermatol"},{"key":"1842_CR24","first-page":"181","volume-title":"Pathology of genetically engineered mice","author":"JP Sundberg","year":"2000","unstructured":"Sundberg JP, King LE (2000) Skin and its appendages: normal anatomy and pathology of spontaneous, transgenic and targeted mouse mutations. In: Ward JM, Mahler JF, Maronpot RR, Sundberg JP (eds) Pathology of genetically engineered mice. Iowa State University Press, Ames, pp 181\u2013213"},{"key":"1842_CR25","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1034\/j.1600-0625.2000.009003206.x","volume":"9","author":"JP Sundberg","year":"2000","unstructured":"Sundberg JP, Boggess D, Bascom C, Limberg BJ, Shultz LD, Sundberg BA, King LE, Montagutelli X (2000) Lanceolate hair-J (lahJ): a mouse model for human hair disorders. Exp Dermatol 9:206\u2013218. https:\/\/doi.org\/10.1034\/j.1600-0625.2000.009003206.x","journal-title":"Exp Dermatol"},{"key":"1842_CR26","doi-asserted-by":"publisher","first-page":"1779","DOI":"10.1038\/sj.jid.5700791","volume":"127","author":"M Wajid","year":"2007","unstructured":"Wajid M, Bazzi H, Rockey J, Lubetkin J, Zlotogorski A, Christiano AM (2007) Localized autosomal recessive hypotrichosis due to a frameshift mutation in the desmoglein 4 gene exhibits extensive phenotypic variability within a Pakistani family. J Invest Dermatol 127:1779\u20131782. https:\/\/doi.org\/10.1038\/sj.jid.5700791","journal-title":"J Invest Dermatol"},{"key":"1842_CR27","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1177\/0192623316631843","volume":"44","author":"MM Welle","year":"2016","unstructured":"Welle MM, Wiener DJ (2016) The hair follicle: a comparative review of canine hair follicle anatomy and physiology. Toxicol Pathol 44:564\u2013574. https:\/\/doi.org\/10.1177\/0192623316631843","journal-title":"Toxicol Pathol"},{"key":"1842_CR28","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1046\/j.1523-1747.2003.12113.x","volume":"120","author":"NV Whittock","year":"2003","unstructured":"Whittock NV, Bower C (2003) Genetic evidence for a novel human desmosomal cadherin, desmoglein 4. J Invest Dermatol 120:523\u2013530. https:\/\/doi.org\/10.1046\/j.1523-1747.2003.12113.x","journal-title":"J Invest Dermatol"},{"key":"1842_CR29","doi-asserted-by":"publisher","first-page":"884","DOI":"10.3390\/genes11080884","volume":"11","author":"DJ Wiener","year":"2020","unstructured":"Wiener DJ, Groch KR, Brunner MAT, Leeb T, Jagannathan V, Welle MM (2020) Transcriptome profiling and differential gene expression in canine microdissected anagen and telogen hair follicles and interfollicular epidermis. Genes (basel) 11:884. https:\/\/doi.org\/10.3390\/genes11080884","journal-title":"Genes (basel)"},{"key":"1842_CR30","doi-asserted-by":"publisher","first-page":"1292","DOI":"10.1038\/sj.jid.5700251","volume":"126","author":"A Zlotogorski","year":"2006","unstructured":"Zlotogorski A, Marek D, Horev L, Abu A, Ben-Amitai D, Gerad L, Ingber A, Frydman M, Reznik-Wolf H, Vardy DA, Pras E (2006) An autosomal recessive form of monilethrix is caused by mutations in DSG4: clinical overlap with localized autosomal recessive hypotrichosis. J Invest Dermatol 126:1292\u20131296. https:\/\/doi.org\/10.1038\/sj.jid.5700251","journal-title":"J Invest Dermatol"}],"container-title":["Molecular Genetics and Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00438-021-01842-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00438-021-01842-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00438-021-01842-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T08:08:57Z","timestamp":1643616537000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00438-021-01842-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,8]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["1842"],"URL":"https:\/\/doi.org\/10.1007\/s00438-021-01842-6","relation":{},"ISSN":["1617-4615","1617-4623"],"issn-type":[{"value":"1617-4615","type":"print"},{"value":"1617-4623","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,8]]},"assertion":[{"value":"22 October 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 November 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 December 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The affected cats in this study were privately owned and skin biopsies and blood samples for diagnostic purposes were collected with the consent of their owners. The collection of blood samples from healthy control cats was approved by the \u201cCantonal Committee for Animal Experiments\u201d (Canton of Bern; permit 71\/19).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}]}}