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Congenital heart defects (CHD) are found in up to 45 percent of girls with TS and span a phenotypic continuum of obstructive left-sided lesions, with bicuspid aortic valve (BAV) being the most common. Several recent studies have demonstrated a genome-wide impact of X chromosome haploinsufficiency, including global hypomethylation and altered RNA expression. The presence of such broad changes to the TS epigenome and transcriptome led others to hypothesize that X chromosome haploinsufficiency sensitizes the TS genome, and several studies have demonstrated that a second genetic hit can modify disease susceptibility in TS. The objective of this study was to determine whether genetic variants in known heart developmental pathways act synergistically in this setting to increase the risk for CHD, specifically BAV, in TS. We analyzed 208 whole exomes from girls and women with TS and performed gene-based variant enrichment analysis and rare-variant association testing to identify variants associated with BAV in TS. Notably, rare variants in\n                    <jats:italic>CRELD1<\/jats:italic>\n                    were significantly enriched in individuals with TS who had BAV compared to those with structurally normal hearts. CRELD1 is a protein that functions as a regulator of calcineurin\/NFAT signaling, and rare variants in\n                    <jats:italic>CRELD1<\/jats:italic>\n                    have been associated with both syndromic and non-syndromic CHD. This observation supports the hypothesis that genetic modifiers outside the X chromosome that lie in known heart development pathways may influence CHD risk in TS.\n                  <\/jats:p>","DOI":"10.1007\/s00439-023-02538-0","type":"journal-article","created":{"date-parts":[[2023,3,26]],"date-time":"2023-03-26T19:51:13Z","timestamp":1679860273000},"page":"523-530","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["CRELD1 variants are associated with bicuspid aortic valve in Turner syndrome"],"prefix":"10.1007","volume":"142","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6450-0918","authenticated-orcid":false,"given":"Catherina T.","family":"Pinnaro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6375-8968","authenticated-orcid":false,"given":"Chloe B.","family":"Beck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather J.","family":"Major","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9279-356X","authenticated-orcid":false,"given":"Benjamin W.","family":"Darbro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,16]]},"reference":[{"issue":"4","key":"2538_CR1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0231402","volume":"15","author":"M Abu-Halima","year":"2020","unstructured":"Abu-Halima M, Oberhoffer FS, El Rahman MA et al (2020) Insights from circulating microRNAs in cardiovascular entities in Turner syndrome patients. 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