{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:39:21Z","timestamp":1791329961677,"version":"4.3.1"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2005,10,25]],"date-time":"2005-10-25T00:00:00Z","timestamp":1130198400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Tissue Res"],"published-print":{"date-parts":[[2006,3]]},"DOI":"10.1007\/s00441-005-0062-7","type":"journal-article","created":{"date-parts":[[2005,10,25]],"date-time":"2005-10-25T09:55:54Z","timestamp":1130234154000},"page":"405-415","source":"Crossref","is-referenced-by-count":112,"title":["Towards an in vitro model of cystic fibrosis small airway epithelium: characterisation of the human bronchial epithelial cell line CFBE41o-"],"prefix":"10.1007","volume":"323","author":[{"given":"Carsten","family":"Ehrhardt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva-Maria","family":"Collnot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Baldes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Laue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Jin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus-Michael","family":"Lehr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2005,10,25]]},"reference":[{"key":"62_CR1","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1164\/rccm.200206-627OC","volume":"166","author":"N Aldallal","year":"2002","unstructured":"Aldallal N, McNaughton EE, Manzel LJ, Richards AM, Zabner J, Ferkol TW, Look DC (2002) Inflammatory response in airway epithelial cells isolated from patients with cystic fibrosis. Am J Respir Crit Care Med 166:1248\u20131256","journal-title":"Am J Respir Crit Care Med"},{"key":"62_CR2","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/S0006-291X(03)01436-0","volume":"308","author":"C Andersson","year":"2003","unstructured":"Andersson C, Servetnyk Z, Roomans GM (2003) Activation of CFTR by genistein in human airway epithelial cell lines. Biochem Biophys Res Commun 308:518\u2013522","journal-title":"Biochem Biophys Res Commun"},{"key":"62_CR3","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1016\/S0169-409X(02)00144-8","volume":"54","author":"RC Boucher","year":"2002","unstructured":"Boucher RC (2002) An overview of the pathogenesis of cystic fibrosis lung disease. Adv Drug Deliv Rev 54:1359\u20131371","journal-title":"Adv Drug Deliv Rev"},{"key":"62_CR4","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/sj.gt.3301741","volume":"9","author":"E Bruscia","year":"2002","unstructured":"Bruscia E, Sangiuolo F, Sinibaldi P, Goncz KK, Novelli G, Gruenert DC (2002) Isolation of CF cell lines corrected at DeltaF508-CFTR locus by SFHR-mediated targeting. Gene Ther 9:683\u2013685","journal-title":"Gene Ther"},{"key":"62_CR5","doi-asserted-by":"crossref","first-page":"5171","DOI":"10.1073\/pnas.89.11.5171","volume":"89","author":"AL Cozens","year":"1992","unstructured":"Cozens AL, Yezzi MJ, Chin L, Simon EM, Finkbeiner WE, Wagner JA, Gruenert DC (1992) Characterization of immortal cystic fibrosis tracheobronchial gland epithelial cells. Proc Natl Acad Sci U S A 89:5171\u20135175","journal-title":"Proc Natl Acad Sci U S A"},{"key":"62_CR6","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1165\/ajrcmb.10.1.7507342","volume":"10","author":"AL Cozens","year":"1994","unstructured":"Cozens AL, Yezzi MJ, Kunzelmann K, Ohrui T, Chin L, Eng K, Finkbeiner WE, Widdicombe JH, Gruenert DC (1994) CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells. Am J Respir Cell Mol Biol 10:38\u201347","journal-title":"Am J Respir Cell Mol Biol"},{"key":"62_CR7","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1023\/A:1003599916558","volume":"31","author":"DR Dorscheid","year":"1999","unstructured":"Dorscheid DR, Conforti AE, Hamann KJ, Rabe KF, White SR (1999) Characterization of cell surface lectin-binding patterns of human airway epithelium. Histochem J 31:145\u2013151","journal-title":"Histochem J"},{"key":"62_CR8","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.1016\/j.lfs.2004.04.025","volume":"75","author":"A Dragomir","year":"2004","unstructured":"Dragomir A, Hjelte L, Hagenfeldt L, Roomans GM (2004) Heparin can improve the viability of transfected cystic fibrosis cell lines in vitro. Life Sci 75:2203\u20132216","journal-title":"Life Sci"},{"key":"62_CR9","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s00441-002-0548-5","volume":"308","author":"C Ehrhardt","year":"2002","unstructured":"Ehrhardt C, Kneuer C, Fiegel J, Hanes J, Schaefer UF, Kim KJ, Lehr CM (2002) Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o-: implications for the use of this cell line as an in vitro model for bronchial drug absorption studies. Cell Tissue Res 308:391\u2013400","journal-title":"Cell Tissue Res"},{"key":"62_CR10","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1023\/A:1023230328687","volume":"20","author":"C Ehrhardt","year":"2003","unstructured":"Ehrhardt C, Kneuer C, Laue M, Schaefer UF, Kim KJ, Lehr CM (2003) 16HBE14o- human bronchial epithelial cell layers express P-glycoprotein, lung resistance-related protein and caveolin-1. Pharm Res 20:545\u2013551","journal-title":"Pharm Res"},{"key":"62_CR11","doi-asserted-by":"crossref","first-page":"6479","DOI":"10.1074\/jbc.272.10.6479","volume":"272","author":"A Fasbender","year":"1997","unstructured":"Fasbender A, Zabner J, Chill\u00f3n M, Moninger TO, Puga AP, Davidson BL, Welsh MJ (1997) Complexes of adenovirus with polycationic polymers and cationic lipids increase the efficiency of gene transfer in vitro and in vivo. J Biol Chem 272:6479\u20136489","journal-title":"J Biol Chem"},{"key":"62_CR12","doi-asserted-by":"crossref","first-page":"E12","DOI":"10.1208\/ps040312","volume":"4","author":"BI Florea","year":"2002","unstructured":"Florea BI, Meaney C, Junginger HE, Borchard G (2002) Transfection efficiency and toxicity of polyethylenimine in differentiated Calu-3 and nondifferentiated COS-1 cell cultures. AAPS PharmSci 4:E12","journal-title":"AAPS PharmSci"},{"key":"62_CR13","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00441-002-0653-5","volume":"311","author":"S Fuchs","year":"2003","unstructured":"Fuchs S, Hollins HJ, Laue M, Schaefer UF, Roemer K, Gumbleton M, Lehr CM (2003) Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C. Cell Tissue Res 311:31\u201345","journal-title":"Cell Tissue Res"},{"key":"62_CR14","doi-asserted-by":"crossref","first-page":"11709","DOI":"10.1074\/jbc.M111958200","volume":"277","author":"MS Gelman","year":"2002","unstructured":"Gelman MS, Kannegaard ES, Kopito RR (2002) A principal role for the proteasome in endoplasmic reticulum-associated degradation of misfolded intracellular cystic fibrosis transmembrane conductance regulator. J Biol Chem 277:11709\u201311714","journal-title":"J Biol Chem"},{"key":"62_CR15","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1093\/hmg\/7.12.1913","volume":"7","author":"KK Goncz","year":"1998","unstructured":"Goncz KK, Kunzelmann K, Xu Z, Gruenert DC (1998) Targeted replacement of normal and mutant CFTR sequences in human airway epithelial cells using DNA fragments. Hum Mol Genet 7:1913\u20131919","journal-title":"Hum Mol Genet"},{"key":"62_CR16","first-page":"489","volume":"5","author":"U Griesenbach","year":"2003","unstructured":"Griesenbach U, Geddes DM, Alton EW (2003) Update on gene therapy for cystic fibrosis. Curr Opin Mol Ther 5:489\u2013494","journal-title":"Curr Opin Mol Ther"},{"key":"62_CR17","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/BF02623833","volume":"26","author":"DC Gruenert","year":"1990","unstructured":"Gruenert DC, Basbaum CB, Widdicombe JH (1990) Long-term culture of normal and cystic fibrosis epithelial cells grown under serum-free conditions. In Vitro Cell Dev Biol 26:411\u2013418","journal-title":"In Vitro Cell Dev Biol"},{"key":"62_CR18","first-page":"L347","volume":"268","author":"DC Gruenert","year":"1995","unstructured":"Gruenert DC, Finkbeiner WE, Widdicombe JH (1995) Culture and transformation of human airway epithelial cells. Am J Physiol 268:L347\u2013L360","journal-title":"Am J Physiol"},{"key":"62_CR19","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1002\/1520-6017(200105)90:5<647::AID-JPS1021>3.0.CO;2-G","volume":"90","author":"KO Hamilton","year":"2001","unstructured":"Hamilton KO, Backstrom G, Yazdanian MA, Audus KL (2001) P-glycoprotein efflux pump expression and activity in Calu-3 cells. J Pharm Sci 90:647\u2013658","journal-title":"J Pharm Sci"},{"key":"62_CR20","first-page":"240","volume":"56","author":"A Jaffe","year":"2001","unstructured":"Jaffe A, Bush A (2001) Cystic fibrosis: review of the decade. Monaldi Arch Chest Dis 56:240\u2013247","journal-title":"Monaldi Arch Chest Dis"},{"key":"62_CR21","first-page":"L496","volume":"259","author":"DM Jefferson","year":"1992","unstructured":"Jefferson DM, Valentich JD, Marini FC, Grubman SA, Iannuzzi MC, Dorkin HL, Klinger KW, Welsh MJ (1992) Expression of normal and cystic fibrosis phenotypes by continuous airway epithelial cell lines. Am J Physiol 259:L496\u2013L505","journal-title":"Am J Physiol"},{"key":"62_CR22","first-page":"17","volume":"59","author":"RG Jenkins","year":"2003","unstructured":"Jenkins RG, McAnulty RJ, Hart SL, Laurent GJ (2003) Pulmonary gene therapy. Realistic hope for the future, or false dawn in the promised land? Monaldi Arch Chest Dis 59:17\u201324","journal-title":"Monaldi Arch Chest Dis"},{"key":"62_CR23","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1165\/ajrcmb.20.6.3440","volume":"20","author":"C Jiand","year":"1999","unstructured":"Jiand C, Finkbeiner WE, Widdicombe JH, Fang SL, Wang KX, Nietupski JB, Hehir KM, Cheng SH (1999) Restoration of cyclic adenosine monophosphate-stimulated chloride channel activity in human cystic fibrosis tracheobronchial submucosal gland cells by adenovirus-mediated and cationic lipid-mediated gene transfer. Am J Respir Cell Mol Biol 20:1107\u20131115","journal-title":"Am J Respir Cell Mol Biol"},{"key":"62_CR24","first-page":"115","volume-title":"Epithelial cell culture protocols","author":"PH Karp","year":"1999","unstructured":"Karp PH, Moninger TO, Weber SP, Nesselhauf TS, Launspach J, Zabner J, Welsh M (1999) Developing an in vitro model of differentiated human airway epithelia: methods for establishing primary cultures. In: Wise C (ed) Epithelial cell culture protocols. Humana, Totowa, pp 115\u2013137"},{"key":"62_CR25","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1183\/09031936.97.10081837","volume":"10","author":"E Lechapt-Zalcman","year":"1997","unstructured":"Lechapt-Zalcman E, Hurbain I, Lacave R, Commo F, Urban T, Antoine M, Milleron B, Bernaudin JF (1997) MDR 1-Pgp 170 expression in human bronchus. Eur Respir J 10:1837\u20131843","journal-title":"Eur Respir J"},{"key":"62_CR26","doi-asserted-by":"crossref","first-page":"27585","DOI":"10.1074\/jbc.C200330200","volume":"277","author":"TW Loo","year":"2002","unstructured":"Loo TW, Bartlett MC, Clarke DM (2002) Introduction of the most common cystic fibrosis mutation (delta F508) into human P-glycoprotein disrupts packing of the transmembrane segments. J Biol Chem 277:27585\u201327588","journal-title":"J Biol Chem"},{"key":"62_CR27","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/S0092-8674(00)81724-9","volume":"95","author":"H Matsui","year":"1998","unstructured":"Matsui H, Grubb BR, Tarran R, Randell SH, Gatzy JT, Davis CW, Boucher RC (1998) Evidence for periciliary liquid layer depletion, not abnormal ion composition, in the pathogenesis of cystic fibrosis airways disease. Cell 95:1005\u20131015","journal-title":"Cell"},{"key":"62_CR28","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s004410051217","volume":"295","author":"GR Newman","year":"1999","unstructured":"Newman GR, Campbell L, von Ruhland C, Jasani B, Gumbleton M (1999) Caveolin and its cellular and subcellular immunolocalisation in lung alveolar epithelium: implications for alveolar epithelial type I cell function. Cell Tissue Res 295:111\u2013120","journal-title":"Cell Tissue Res"},{"key":"62_CR29","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1007\/BF03256668","volume":"2","author":"GM Roomans","year":"2003","unstructured":"Roomans GM (2003) Pharmacological approaches to correcting the ion transport defect in cystic fibrosis. Am J Respir Med 2:413\u2013431","journal-title":"Am J Respir Med"},{"key":"62_CR30","doi-asserted-by":"crossref","first-page":"39051","DOI":"10.1074\/jbc.M407434200","volume":"279","author":"MF Rosenberg","year":"2004","unstructured":"Rosenberg MF, Kamis AB, Aleksandrov LA, Ford RC, Riordan JR (2004) Purification and crystallisation of the cystic fibrosis transmembrane conductance regulator (CFTR). J Biol Chem 279:39051\u201339057","journal-title":"J Biol Chem"},{"key":"62_CR31","first-page":"66","volume":"102","author":"S Rybarova","year":"2001","unstructured":"Rybarova S, Batekova M, Hodorova I, Mirossay A, Kluchova D, Bobrov N, Kocisova M (2001) Immunohistochemical detection of LRP protein in the normal human lung. Bratisl Lek L 102:66\u201372","journal-title":"Bratisl Lek L"},{"key":"62_CR32","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1471-2350-3-8","volume":"3","author":"F Sangiuolo","year":"2002","unstructured":"Sangiuolo F, Bruscia E, Serafino A, Nardone AM, Bonifazi E, Lais M, Gruenert DC, Novelli G (2002) In vitro correction of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique. BMC Med Genet 3:8","journal-title":"BMC Med Genet"},{"key":"62_CR33","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.jconrel.2004.02.029","volume":"97","author":"K Tabatt","year":"2004","unstructured":"Tabatt K, Kneuer C, Sameti M, Olbrich C, Muller RH, Lehr CM, Bakowsky U (2004) Transfection with different colloidal systems: comparison of solid lipid nanoparticles and liposomes. J Control Release 97:321\u2013332","journal-title":"J Control Release"},{"key":"62_CR34","doi-asserted-by":"crossref","first-page":"C791","DOI":"10.1152\/ajpcell.00435.2002","volume":"284","author":"TA Tucker","year":"2003","unstructured":"Tucker TA, Varga K, Bebok Z, Zsembery A, McCarty NA, Collawn JF, Schwiebert EM, Schwiebert LM (2003) Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipids. Am J Physiol 284:C791\u2013C804","journal-title":"Am J Physiol"},{"key":"62_CR35","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1165\/ajrcmb\/4.4.313","volume":"4","author":"PL Zeitlin","year":"1991","unstructured":"Zeitlin PL, Lu L, Rhim J, Cutting G, Stetten G, Kieffer KA, Craig R, Guggino WB (1991) A cystic fibrosis bronchial epithelial cell line: immortalization by adeno-12-SV40 infection. Am J Respir Cell Mol Biol 4:313\u2013319","journal-title":"Am J Respir Cell Mol Biol"}],"container-title":["Cell and Tissue Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-005-0062-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00441-005-0062-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-005-0062-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T23:41:08Z","timestamp":1559086868000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00441-005-0062-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10,25]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["62"],"URL":"https:\/\/doi.org\/10.1007\/s00441-005-0062-7","relation":{},"ISSN":["0302-766X","1432-0878"],"issn-type":[{"value":"0302-766X","type":"print"},{"value":"1432-0878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,10,25]]}}}