{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T18:37:44Z","timestamp":1780079864845,"version":"3.54.0"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2010,5,7]],"date-time":"2010-05-07T00:00:00Z","timestamp":1273190400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Tissue Res"],"published-print":{"date-parts":[[2010,6]]},"DOI":"10.1007\/s00441-010-0972-x","type":"journal-article","created":{"date-parts":[[2010,5,6]],"date-time":"2010-05-06T08:39:42Z","timestamp":1273135182000},"page":"569-581","source":"Crossref","is-referenced-by-count":25,"title":["Altered epididymal sperm maturation and cytoplasmic droplet migration in subfertile male Alox15 mice"],"prefix":"10.1007","volume":"340","author":[{"given":"Kelly","family":"Moore","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle","family":"Lovercamp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Antelman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Sutovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurishankar","family":"Manandhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"van Leyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Safranski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Sutovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,5,7]]},"reference":[{"key":"972_CR1","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1530\/jrf.0.1200385","volume":"120","author":"MA Akbarsha","year":"2000","unstructured":"Akbarsha MA, Latha PN, Murugaian P (2000) Retention of cytoplasmic droplet by rat cauda epididymal spermatozoa after treatment with cytotoxic and xenobiotic agents. J Reprod Fertil 120:385\u2013390","journal-title":"J Reprod Fertil"},{"key":"972_CR2","volume-title":"Abnormal morphology of bovine spermatozoa.","author":"AD Barth","year":"1989","unstructured":"Barth AD, Oko RJ (1989) Abnormal morphology of bovine spermatozoa. Iowa State University Press, Ames, Iowa"},{"key":"972_CR3","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1007\/BF00381652","volume":"55","author":"G Bloom","year":"1961","unstructured":"Bloom G, Nicander L (1961) On the ultrastructure and development of the protoplasmic droplet of spermatozoa. Z Zellforsch 55:833\u2013844","journal-title":"Z Zellforsch"},{"key":"972_CR4","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/BF00210003","volume":"219","author":"SA Bozek","year":"1981","unstructured":"Bozek SA, Jensen DR, Tone JN (1981) Scanning electron microscopic study of spermatozoa from gossypol-treated rats. Cell Tissue Res 219:659\u2013663","journal-title":"Cell Tissue Res"},{"key":"972_CR5","doi-asserted-by":"crossref","first-page":"23679","DOI":"10.1074\/jbc.274.34.23679","volume":"274","author":"AR Brash","year":"1999","unstructured":"Brash AR (1999) Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate. J Biol Chem 274:23679\u201323682","journal-title":"J Biol Chem"},{"key":"972_CR6","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/molehr\/3.3.195","volume":"3","author":"H Breitbart","year":"1997","unstructured":"Breitbart H, Spungin B (1997) The biochemistry of the acrosome reaction. Mol Hum Reprod 3:195\u2013202","journal-title":"Mol Hum Reprod"},{"key":"972_CR7","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1016\/S0093-691X(00)00264-8","volume":"53","author":"PJ Chenoweth","year":"2000","unstructured":"Chenoweth PJ, Chase CC Jr, Risco CA, Larsen RE (2000) Characterization of gossypol-induced sperm abnormalities in bulls. Theriogenology 53:1193\u20131203","journal-title":"Theriogenology"},{"key":"972_CR8","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/jemt.10314","volume":"61","author":"TG Cooper","year":"2003","unstructured":"Cooper TG, Yeung CH (2003) Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet. Microsc Res Tech 61:28\u201338","journal-title":"Microsc Res Tech"},{"key":"972_CR9","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/jemt.10312","volume":"61","author":"JL Dacheux","year":"2003","unstructured":"Dacheux JL, Gatti JL, Dacheux F (2003) Contribution of epididymal secretory proteins for spermatozoa maturation. Microsc Res Tech 61:7\u201317","journal-title":"Microsc Res Tech"},{"key":"972_CR10","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.mce.2009.03.007","volume":"306","author":"JL Dacheux","year":"2009","unstructured":"Dacheux JL, Belleannee C, Jones R, Labas V, Belghazi M, Guyonnet B, Druart X, Gatti JL, Dacheux F (2009) Mammalian epididymal proteome. Mol Cell Endocrinol 306:45\u201350","journal-title":"Mol Cell Endocrinol"},{"key":"972_CR11","first-page":"505","volume":"21","author":"W Dubiel","year":"1989","unstructured":"Dubiel W, Rapoport SM (1989) ATP-dependent proteolysis of mitochondria of reticulocytes. Revis Biol Celular 21:505\u2013521","journal-title":"Revis Biol Celular"},{"key":"972_CR12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1530\/rep.1.00646","volume":"130","author":"KA Fischer","year":"2005","unstructured":"Fischer KA, Leyen K van, Lovercamp KW, Manandhar G, Sutovsky M, Feng D, Safranski T, Sutovsky P (2005) 15-Lipoxygenase is a component of the mammalian sperm cytoplasmic droplet. Reproduction 130:213\u2013222","journal-title":"Reproduction"},{"key":"972_CR13","first-page":"65","volume":"4","author":"AW Ford-Hutchinson","year":"1991","unstructured":"Ford-Hutchinson AW (1991) Arachidonate 15-lipoxygenase; characteristics and potential biological significance. Eicosanoids 4:65\u201374","journal-title":"Eicosanoids"},{"key":"972_CR14","first-page":"241","volume":"16","author":"S Grossman","year":"1986","unstructured":"Grossman S, Schon IS, Sofer Y, Magid N, Bartoov B (1986) Arachidonic acid metabolizing enzymes in the ram reproductive system. Adv Prostaglandin Thromboxane Leukot Res 16:241\u2013252","journal-title":"Adv Prostaglandin Thromboxane Leukot Res"},{"key":"972_CR15","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0014-5793(01)02080-4","volume":"489","author":"C Grullich","year":"2001","unstructured":"Grullich C, Duvoisin RM, Wiedmann M, Leyen K van (2001) Inhibition of 15-lipoxygenase leads to delayed organelle degradation in the reticulocyte. FEBS Lett 489:51\u201354","journal-title":"FEBS Lett"},{"key":"972_CR16","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0005-2760(85)90173-0","volume":"834","author":"Y Hamasaki","year":"1985","unstructured":"Hamasaki Y, Tai HH (1985) Gossypol, a potent inhibitor of arachidonate 5- and 12-lipoxygenases. Biochim Biophys Acta 834:37\u201341","journal-title":"Biochim Biophys Acta"},{"key":"972_CR17","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1071\/RD9961039","volume":"8","author":"H Harayama","year":"1996","unstructured":"Harayama H, Shibukawa T, Miyake M, Kannan Y, Kato S (1996) Fructose stimulates shedding of cytoplasmic droplets from epididymal boar spermatozoa. Reprod Fertil Dev 8:1039\u20131043","journal-title":"Reprod Fertil Dev"},{"key":"972_CR18","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1002\/aja.1001830202","volume":"183","author":"L Hermo","year":"1988","unstructured":"Hermo L, Dworkin J, Oko R (1988) Role of epithelial clear cells of the rat epididymis in the disposal of the contents of cytoplasmic droplets detached from spermatozoa. Am J Anat 183:107\u2013124","journal-title":"Am J Anat"},{"key":"972_CR19","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1016\/S0021-9258(19)40037-9","volume":"265","author":"H Kuhn","year":"1990","unstructured":"Kuhn H, Brash AR (1990) Occurrence of lipoxygenase products in membranes of rabbit reticulocytes. Evidence for a role of the reticulocyte lipoxygenase in the maturation of red cells. J Biol Chem 265:1454\u20131458","journal-title":"J Biol Chem"},{"key":"972_CR20","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1002\/j.1939-4640.2004.tb02798.x","volume":"25","author":"CE Kuster","year":"2004","unstructured":"Kuster CE, Hess RA, Althouse GC (2004) Immunofluorescence reveals ubiquitination of retained distal cytoplasmic droplets on ejaculated porcine spermatozoa. J Androl 25:340\u2013347","journal-title":"J Androl"},{"key":"972_CR21","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1095\/biolreprod.109.079566","volume":"82","author":"S Kwitny","year":"2009","unstructured":"Kwitny S, Klaus AV, Hunnicutt GR (2009) The annulus of the mouse sperm tail is required to establish a membrane diffusion barrier that is engaged during the late steps of spermiogenesis. Biol Reprod 82:669\u2013678","journal-title":"Biol Reprod"},{"key":"972_CR22","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/0005-2760(90)90104-6","volume":"1043","author":"Y Lax","year":"1990","unstructured":"Lax Y, Grossman S, Rubinstein S, Magid N, Breitbart H (1990) Role of lipoxygenase in the mechanism of acrosome reaction in mammalian spermatozoa. Biochim Biophys Acta 1043:12\u201318","journal-title":"Biochim Biophys Acta"},{"key":"972_CR23","first-page":"189","volume":"5","author":"GZ Liu","year":"1981","unstructured":"Liu GZ (1981) Clinical study of gossypol as a male contraceptive. Reproduction 5:189\u2013193","journal-title":"Reproduction"},{"key":"972_CR24","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/j.theriogenology.2006.08.019","volume":"67","author":"KW Lovercamp","year":"2007","unstructured":"Lovercamp KW, Safranski TJ, Fischer KA, Manandhar G, Sutovsky M, Herring W, Sutovsky P (2007) Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars. Theriogenology 67:704\u2013718","journal-title":"Theriogenology"},{"key":"972_CR25","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/aja.1001740309","volume":"174","author":"S Meizel","year":"1985","unstructured":"Meizel S (1985) Molecules that initiate or help stimulate the acrosome reaction by their interaction with the mammalian sperm surface. Am J Anat 174:285\u2013302","journal-title":"Am J Anat"},{"key":"972_CR26","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1083\/jcb.123.4.809","volume":"123","author":"R Oko","year":"1993","unstructured":"Oko R, Hermo L, Chan PT, Fazel A, Bergeron JJ (1993) The cytoplasmic droplet of rat epididymal spermatozoa contains saccular elements with Golgi characteristics. J Cell Biol 123:809\u2013821","journal-title":"J Cell Biol"},{"key":"972_CR27","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0005-2760(89)90342-1","volume":"1002","author":"EH Oliw","year":"1989","unstructured":"Oliw EH, Sprecher H (1989) Metabolism of polyunsaturated fatty acids by an (n-6)-lipoxygenase associated with human ejaculates. Biochim Biophys Acta 1002:283\u2013291","journal-title":"Biochim Biophys Acta"},{"key":"972_CR28","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1002\/jemt.10316","volume":"61","author":"GE Olson","year":"2003","unstructured":"Olson GE, Winfrey VP, Nagdas SK (2003) Structural modification of the hamster sperm acrosome during posttesticular development in the epididymis. Microsc Res Tech 61:46\u201355","journal-title":"Microsc Res Tech"},{"key":"972_CR29","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1530\/rep.0.1210889","volume":"121","author":"AM Petrunkina","year":"2001","unstructured":"Petrunkina AM, Gehlhaar R, Drommer W, Waberski D, Topfer-Petersen E (2001) Selective sperm binding to pig oviductal epithelium in vitro. Reproduction 121:889\u2013896","journal-title":"Reproduction"},{"key":"972_CR30","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.ydbio.2007.09.053","volume":"312","author":"CI Rodriguez","year":"2007","unstructured":"Rodriguez CI, Stewart CL (2007) Disruption of the ubiquitin ligase HERC4 causes defects in spermatozoon maturation and impaired fertility. Dev Biol 312:501\u2013508","journal-title":"Dev Biol"},{"key":"972_CR31","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0014-5793(75)80439-X","volume":"60","author":"T Schewe","year":"1975","unstructured":"Schewe T, Halangk W, Hiebsch C, Rapoport SM (1975) A lipoxygenase in rabbit reticulocytes which attacks phospholipids and intact mitochondria. FEBS Lett 60:149\u2013152","journal-title":"FEBS Lett"},{"key":"972_CR32","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1002\/j.1939-4640.1985.tb00814.x","volume":"6","author":"QX Shi","year":"1985","unstructured":"Shi QX, Friend DS (1985) Effect of gossypol acetate on guinea pig epididymal spermatozoa in vivo and their susceptibility to capacitation in vitro. J Androl 6:45\u201352","journal-title":"J Androl"},{"key":"972_CR33","doi-asserted-by":"crossref","first-page":"24055","DOI":"10.1074\/jbc.271.39.24055","volume":"271","author":"D Sun","year":"1996","unstructured":"Sun D, Funk CD (1996) Disruption of 12\/15-lipoxygenase expression in peritoneal macrophages. Enhanced utilization of the 5-lipoxygenase pathway and diminished oxidation of low density lipoprotein. J Biol Chem 271:24055\u201324062","journal-title":"J Biol Chem"},{"key":"972_CR34","first-page":"59","volume":"253","author":"P Sutovsky","year":"2004","unstructured":"Sutovsky P (2004) Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa, and zygotes by immunofluorescence, confocal, and immunoelectron microscopy. Methods Mol Biol 253:59\u201377","journal-title":"Methods Mol Biol"},{"key":"972_CR35","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/S1472-6483(10)60495-6","volume":"8","author":"P Sutovsky","year":"2004","unstructured":"Sutovsky P, Leyen K van, McCauley T, Day BN, Sutovsky M (2004) Degradation of paternal mitochondria after fertilization: implications for heteroplasmy, assisted reproductive technologies and mtDNA inheritance. Reprod Biomed Online 8:24\u201333","journal-title":"Reprod Biomed Online"},{"key":"972_CR36","doi-asserted-by":"crossref","first-page":"3070","DOI":"10.1210\/endo.136.7.7789334","volume":"136","author":"V Syed","year":"1995","unstructured":"Syed V, Stephan JP, Gerard N, Legrand A, Parvinen M, Bardin CW, Jegou B (1995) Residual bodies activate Sertoli cell interleukin-1 alpha (IL-1 alpha) release, which triggers IL-6 production by an autocrine mechanism, through the lipoxygenase pathway. Endocrinology 136:3070\u20133078","journal-title":"Endocrinology"},{"key":"972_CR37","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0378-4320(00)00231-1","volume":"65","author":"J Thundathil","year":"2001","unstructured":"Thundathil J, Palasz AT, Barth AD, Mapletoft RJ (2001) The use of in vitro fertilization techniques to investigate the fertilizing ability of bovine sperm with proximal cytoplasmic droplets. Anim Reprod Sci 65:181\u2013192","journal-title":"Anim Reprod Sci"},{"key":"972_CR38","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/S0962-8924(98)01386-5","volume":"8","author":"K Leyen van","year":"1998","unstructured":"van Leyen K (1998) Pictures in cell biology. Un-LOXing the door to organelle degradation. Trends Cell Biol 8:436","journal-title":"Trends Cell Biol"},{"key":"972_CR39","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0378-4320(94)90061-2","volume":"36","author":"D Waberski","year":"1994","unstructured":"Waberski D, Meding S, Dirksen G, Weitze KF, Leiding C, Hahn R (1994) Fertility of long-term-stored boar semen: influence of extender (Androhep and Kiev), storage time and plasma droplets in the semen. Anim Reprod Sci 36:145\u2013151","journal-title":"Anim Reprod Sci"},{"key":"972_CR40","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1002\/jemt.10315","volume":"61","author":"K Yoshinaga","year":"2003","unstructured":"Yoshinaga K, Toshimori K (2003) Organization and modifications of sperm acrosomal molecules during spermatogenesis and epididymal maturation. Microsc Res Tech 61:39\u201345","journal-title":"Microsc Res Tech"},{"key":"972_CR41","doi-asserted-by":"crossref","first-page":"6852","DOI":"10.1073\/pnas.0701669104","volume":"104","author":"H Zheng","year":"2007","unstructured":"Zheng H, Stratton CJ, Morozumi K, Jin J, Yanagimachi R, Yan W (2007) Lack of Spem1 causes aberrant cytoplasm removal, sperm deformation, and male infertility. Proc Natl Acad Sci USA 104:6852\u20136857","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["Cell and Tissue Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-010-0972-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00441-010-0972-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-010-0972-x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T06:48:14Z","timestamp":1635317294000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00441-010-0972-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,7]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["972"],"URL":"https:\/\/doi.org\/10.1007\/s00441-010-0972-x","relation":{},"ISSN":["0302-766X","1432-0878"],"issn-type":[{"value":"0302-766X","type":"print"},{"value":"1432-0878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,7]]}}}