{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:09:46Z","timestamp":1787317786081,"version":"build-2736575974"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2019,9,7]],"date-time":"2019-09-07T00:00:00Z","timestamp":1567814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2019,9,7]],"date-time":"2019-09-07T00:00:00Z","timestamp":1567814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Tissue Res"],"published-print":{"date-parts":[[2020,3]]},"DOI":"10.1007\/s00441-019-03092-w","type":"journal-article","created":{"date-parts":[[2019,9,7]],"date-time":"2019-09-07T01:55:03Z","timestamp":1567821303000},"page":"577-587","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Sertoli cell\u2013conditioned medium restores spermatogenesis in azoospermic mouse testis"],"prefix":"10.1007","volume":"379","author":[{"given":"Sakineh","family":"Panahi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Karamian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ensieh","family":"Sajadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas","family":"Aliaghaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Nazarian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabnam","family":"Abdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Danyali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahrokh","family":"Paktinat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6947-3285","authenticated-orcid":false,"given":"Mohammad-Amin","family":"Abdollahifar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza Mastery","family":"Farahani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,7]]},"reference":[{"issue":"2","key":"3092_CR1","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1530\/jrf.0.0850771","volume":"85","author":"A Bellve","year":"1989","unstructured":"Bellve A, Zheng W (1989) Growth factors as autocrine and paracrine modulators of male gonadal functions. J Reprod Fertil 85(2):771\u2013793","journal-title":"J Reprod Fertil"},{"key":"3092_CR2","doi-asserted-by":"crossref","unstructured":"Endo T, Romer KA, Anderson EL, Baltus AE, de Rooij DG, Page DC (2015) \"Periodic retinoic acid\u2013STRA8 signaling intersects with periodic germ-cell competencies to regulate spermatogenesis.\" Proc Natl Acad Sci: 201505683","DOI":"10.1073\/pnas.1505683112"},{"issue":"3","key":"3092_CR3","doi-asserted-by":"publisher","first-page":"e12887","DOI":"10.1111\/and.12887","volume":"50","author":"R Ghaem Maghami","year":"2018","unstructured":"Ghaem Maghami R, Mirzapour T, Bayrami A (2018) Differentiation of mesenchymal stem cells to germ-like cells under induction of Sertoli cell-conditioned medium and retinoic acid. Andrologia 50(3):e12887","journal-title":"Andrologia"},{"issue":"1","key":"3092_CR4","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/0303-7207(90)90064-F","volume":"70","author":"A Grootenhuis","year":"1990","unstructured":"Grootenhuis A, Timmerman M, Hordijk P, de Jong F (1990) Inhibin in immature rat Sertoli cell conditioned medium: a 32 kDa \u03b1\u03b2-B dimer. Mol Cell Endocrinol 70(1):109\u2013116","journal-title":"Mol Cell Endocrinol"},{"issue":"7\u201312","key":"3092_CR5","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1111\/j.1699-0463.1988.tb00954.x","volume":"96","author":"H Gundersen","year":"1988","unstructured":"Gundersen H, Bagger P, Bendtsen T, Evans S, Korbo L, Marcussen N, M\u00f8ller A, Nielsen K, Nyengaard J, Pakkenberg B (1988a) The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. Apmis 96(7\u201312):857\u2013881","journal-title":"Apmis"},{"issue":"1\u20136","key":"3092_CR6","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1111\/j.1699-0463.1988.tb05320.x","volume":"96","author":"H Gundersen","year":"1988","unstructured":"Gundersen H, BENDTSEN TF, KORBO L, MARCUSSEN N, M\u00f8ller A, Nielsen K, Nyengaard J, Pakkenberg B, S\u00f8rensen FB, Vesterby A (1988b) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Apmis 96(1\u20136):379\u2013394","journal-title":"Apmis"},{"issue":"4","key":"3092_CR7","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.4067\/S0717-95022017000401597","volume":"35","author":"M Hajian Monfared","year":"2017","unstructured":"Hajian Monfared M, Akbari M, Solhjoo S, Tooli H, Omidi A, Aliakbari F, Ijaz S, Mokhtari T, Rastegar T (2017) Inductive role of sustentacular cells (Sertoli cells) conditioned medium on bone marrow derived mesenchymal stem cells. Int J Morphol 35(4):1597\u20131606","journal-title":"Int J Morphol"},{"key":"3092_CR8","doi-asserted-by":"crossref","unstructured":"Hajihoseini M, Vahdati A, Ebrahim Hosseini S, Mehrabani D, Tamadon A (2017) Induction of spermatogenesis after stem cell therapy of azoospermic guinea pigs. Vet Arh 87(3):333\u2013350","DOI":"10.24099\/vet.arhiv.151209"},{"key":"3092_CR9","doi-asserted-by":"crossref","unstructured":"Jarvi K, Lo K, Fischer A, Grantmyre J, Zini A, Chow V, Mak V (2010) CUA Guideline: The workup of azoospermic males. Can Urol Assoc J 4(3):163","DOI":"10.5489\/cuaj.10050"},{"key":"3092_CR10","doi-asserted-by":"crossref","unstructured":"Ma H-T, Niu C-M, Xia J, Shen X-Y, Xia M-M, Hu Y-Q, Zheng Y (2018) Stimulated by retinoic acid gene 8 (Stra8) plays important roles in many stages of spermatogenesis. Asian J Androl 20(5):479","DOI":"10.4103\/aja.aja_26_18"},{"key":"3092_CR11","doi-asserted-by":"crossref","unstructured":"Medrano J, Marques-Mari A, Aguilar C, Riboldi M, Garrido N, Martinez-Romero A, O'connor E, Gil-Salom M, Simon C (2010) Comparative analysis of the germ cell markers c-KIT, SSEA-1 and VASA in testicular biopsies from secretory and obstructive azoospermias. Mol Hum Reprod 16(11):811\u2013817","DOI":"10.1093\/molehr\/gaq044"},{"key":"3092_CR12","unstructured":"Monfared MH, Minaee B, Rastegar T, Khrazinejad E, Barbarestani M (2016) Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells. Iran J Basic Med Sci 19(11):1186"},{"key":"3092_CR13","unstructured":"Monsefi M, Fereydouni B, Rohani L, Talaei T (2013) Mesenchymal stem cells repair germinal cells of seminiferous tubules of sterile rats. Iran J Reprod Med 11(7):537"},{"key":"3092_CR14","doi-asserted-by":"crossref","unstructured":"Mozafar A, Mehrabani D, Vahdati A, Hosseini E, Forouzanfar M (2018) Histomorphometric evaluation of allogeneic transplantation of bone marrow mesenchymal stem cells in azoospermic mice model. lnaBJ 10(2):171\u2013178","DOI":"10.18585\/inabj.v10i2.395"},{"issue":"7","key":"3092_CR15","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1038\/labinvest.3700429","volume":"86","author":"K Nayernia","year":"2006","unstructured":"Nayernia K, Lee JH, Drusenheimer N, Nolte J, Wulf G, Dressel R, Gromoll J, Engel W (2006) Derivation of male germ cells from bone marrow stem cells. Lab Investig 86(7):654","journal-title":"Lab Investig"},{"issue":"4","key":"3092_CR16","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1530\/REP-14-0653","volume":"149","author":"BA Niedenberger","year":"2015","unstructured":"Niedenberger BA, Busada JT, Geyer CB (2015) Marker expression reveals heterogeneity of spermatogonia in the neonatal mouse testis. Reproduction 149(4):329\u2013338","journal-title":"Reproduction"},{"issue":"3","key":"3092_CR17","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/0303-7207(87)90017-7","volume":"50","author":"M Perrard-Sapori","year":"1987","unstructured":"Perrard-Sapori M, Chatelain P, Rogemond N, Saez J (1987) Modulation of Leydig cell functions by culture with Sertoli cells or with Sertoli cell-conditioned medium: effect of insulin, somatomedin-C and FSH. Mol Cell Endocrinol 50(3):193\u2013201","journal-title":"Mol Cell Endocrinol"},{"issue":"9","key":"3092_CR18","doi-asserted-by":"publisher","first-page":"e36","DOI":"10.1093\/nar\/30.9.e36","volume":"30","author":"MW Pfaffl","year":"2002","unstructured":"Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST\u00a9) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30(9):e36\u2013e36","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"3092_CR19","doi-asserted-by":"publisher","first-page":"2479","DOI":"10.1210\/en.2016-1156","volume":"157","author":"D Rebourcet","year":"2016","unstructured":"Rebourcet D, Wu J, Cruickshanks L, Smith SE, Milne L, Fernando A, Wallace RJ, Gray CD, Hadoke PW, Mitchell RT (2016) Sertoli cells modulate testicular vascular network development, structure, and function to influence circulating testosterone concentrations in adult male mice. Endocrinology 157(6):2479\u20132488","journal-title":"Endocrinology"},{"issue":"3","key":"3092_CR20","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/j.modgep.2003.11.003","volume":"4","author":"P Rossi","year":"2004","unstructured":"Rossi P, Dolci S, Sette C, Capolunghi F, Pellegrini M, Loiarro M, Di Agostino S, Paronetto MP, Grimaldi P, Merico D (2004) Analysis of the gene expression profile of mouse male meiotic germ cells. Gene Expr Patterns 4(3):267\u2013281","journal-title":"Gene Expr Patterns"},{"key":"3092_CR21","doi-asserted-by":"crossref","unstructured":"Takashima S, Kanatsu-Shinohara M, Tanaka T, Morimoto H, Inoue K, Ogonuki N, Jijiwa M, Takahashi M, Ogura A, Shinohara T (2015) Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal. Stem Cell Reports 4(3):489\u2013502","DOI":"10.1016\/j.stemcr.2015.01.010"},{"key":"3092_CR22","unstructured":"Zanganeh BM, Rastegar T, Roudkenar MH, Kashani IR, Amidi F, Barbarestani M (2013) Co-culture of spermatogonial stem cells with sertoli cells in the presence of testosterone and FSH improved differentiation via up-regulation of post meiotic genes. Acta Med Iran 51(1):1\u201311"},{"issue":"3","key":"3092_CR23","doi-asserted-by":"publisher","first-page":"186","DOI":"10.4161\/spmg.1.3.17760","volume":"1","author":"L Zhang","year":"2011","unstructured":"Zhang L, Tang J, Haines CJ, Feng H, Lai L, Teng X, Han Y (2011) c-kit and its related genes in spermatogonial differentiation. Spermatogenesis 1(3):186\u2013194","journal-title":"Spermatogenesis"},{"issue":"1","key":"3092_CR24","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/1471-213X-13-38","volume":"13","author":"L Zhang","year":"2013","unstructured":"Zhang L, Tang J, Haines CJ, Feng H, Lai L, Teng X, Han Y (2013) c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation. BMC Dev Biol 13(1):38","journal-title":"BMC Dev Biol"}],"updated-by":[{"DOI":"10.1007\/s00441-025-03986-y","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000}}],"container-title":["Cell and Tissue Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-019-03092-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00441-019-03092-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-019-03092-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T05:34:07Z","timestamp":1751002447000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00441-019-03092-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,7]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["3092"],"URL":"https:\/\/doi.org\/10.1007\/s00441-019-03092-w","relation":{},"ISSN":["0302-766X","1432-0878"],"issn-type":[{"value":"0302-766X","type":"print"},{"value":"1432-0878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,7]]},"assertion":[{"value":"12 January 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 August 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 September 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 June 2025","order":4,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Update","order":5,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The original online version of this article was revised: \nThe authors regret that the version of Figures 2, 6, and 9 that appeared in the original published article are incorrect. \nThe incorrect and correct version of Figures 2, 6, and 9 are shown in the erratum article. The full original dataset is also provided as a Supplementary Material in the original article.","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The original article has been corrected.","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 June 2025","order":8,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Correction","order":9,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"A Correction to this paper has been published:","order":10,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"https:\/\/doi.org\/10.1007\/s00441-025-03986-y","URL":"https:\/\/doi.org\/10.1007\/s00441-025-03986-y","order":11,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The experimental protocol and all procedures performed in studies involving the use of animals were reviewed and approved by the Ethical Committee at Shahid Beheshti University of Medical Sciences (IR. SBMU. SM.REC.1396.184).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}