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But not all flatworms can regenerate equally well and to obtain a better picture of the characteristics and evolution of regeneration in flatworms other than planarians, the regeneration capacity and stem cell dynamics during regeneration in the flatworm order Polycladida are studied. Here, we show that as long as the brain remained at least partially intact, the polyclad <jats:italic>Prosthiostomum siphunculus<\/jats:italic> was able to regenerate submarginal eyes, cerebral eyes, pharynx, intestine and sucker. In the complete absence of the brain only wound closure was observed but no regeneration of missing organs. Amputated parts of the brain could not be regenerated. The overall regeneration capacity of <jats:italic>P. siphunculus<\/jats:italic> is a good fit for category III after a recently established system, in which most polyclads are currently classified. Intact animals showed proliferating cells in front of the brain which is an exception compared with most of the other free-living flatworms that have been observed so far. Proliferating cells could be found within the regeneration blastema, similar to all other flatworm taxa except triclads. No proliferation was observed in epidermis and pharynx. In pulse-chase experiments, the chased cells were found in all regenerated tissues and thereby shown to differentiate and migrate to replace the structures lost upon amputation.<\/jats:p>","DOI":"10.1007\/s00441-020-03302-w","type":"journal-article","created":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T15:02:44Z","timestamp":1604761364000},"page":"1025-1041","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Regeneration of the flatworm Prosthiostomum siphunculus (Polycladida, Platyhelminthes)"],"prefix":"10.1007","volume":"383","author":[{"given":"Tamara","family":"Schadt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Prantl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra L","family":"Grosbusch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"Bertemes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6353-9458","authenticated-orcid":false,"given":"Bernhard","family":"Egger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,11,7]]},"reference":[{"issue":"2","key":"3302_CR1","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1007\/s00441-019-03094-8","volume":"379","author":"P Bertemes","year":"2020","unstructured":"Bertemes P, Grosbusch AL, Egger B (2020) No head regeneration here: regeneration capacity and stem cell dynamics of Theama mediterranea (Polycladida, Platyhelminthes). 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