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Here, we investigated <jats:italic>Ccn2\/Ctgf<\/jats:italic> promotor activity during development and in the adult mouse eye, using <jats:italic>CTGF<\/jats:italic><jats:sup><jats:italic>LacZ\/<\/jats:italic>+<\/jats:sup> mice in which the \u03b2-galactosidase reporter gene <jats:italic>LacZ<\/jats:italic> had been inserted into the open reading frame of <jats:italic>Ccn2\/Ctgf<\/jats:italic>. Promotor activity was assessed by staining for \u03b2-galactosidase activity and by immunolabeling using antibodies against \u03b2-galactosidase. Co-immunostaining using antibodies against glutamine synthetase, glial fibrillary acidic protein, choline acetyltransferase, and CD31 was applied to identify specific cell types. <jats:italic>Ccn2\/Ctgf<\/jats:italic> promotor activity was intense in neural crest-derived cells differentiating to corneal stroma and endothelium, and to the stroma of choroid, iris, ciliary body, and the trabecular meshwork during development. In the adult eye, a persistent and very strong promotor activity was present in the trabecular meshwork outflow pathways. In addition, endothelial cells of Schlemm\u2019s canal, and of retinal and choroidal vessels, retinal astrocytes, M\u00fcller glia, and starburst amacrine cells were stained. Very strong promoter activity was seen in the astrocytes of the glial lamina at the optic nerve head. We conclude that CCN2\/CTGF signaling is involved in the processes that govern neural crest morphogenesis during ocular development. In the adult eye, CCN2\/CTGF likely plays an important role for the trabecular meshwork outflow pathways and the glial lamina of the optic nerve head.<\/jats:p>","DOI":"10.1007\/s00441-020-03332-4","type":"journal-article","created":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T15:03:36Z","timestamp":1611932616000},"page":"625-641","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["CCN2\/CTGF promotor activity in the developing and adult mouse eye"],"prefix":"10.1007","volume":"384","author":[{"given":"Andrea E.","family":"Dillinger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Kuespert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franziska","family":"Froemel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernst R.","family":"Tamm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0474-6980","authenticated-orcid":false,"given":"Rudolf","family":"Fuchshofer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,1,29]]},"reference":[{"key":"3332_CR1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/ncb826","volume":"4","author":"JG Abreu","year":"2002","unstructured":"Abreu JG, Ketpura NI, Reversade B, De Robertis EM (2002) Connective-tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-beta. 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