{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:59:51Z","timestamp":1790895591002,"version":"4.1.0"},"reference-count":81,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/100001515","name":"Britton Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001515","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008564","name":"School of Medicine, Case Western Reserve University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Tissue Res"],"published-print":{"date-parts":[[2023,4]]},"DOI":"10.1007\/s00441-022-03595-z","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T09:03:02Z","timestamp":1646730182000},"page":"323-335","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Seed amplification and RT-QuIC assays to investigate protein seed structures and strains"],"prefix":"10.1007","volume":"392","author":[{"given":"Heidi G.","family":"Standke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8477-0894","authenticated-orcid":false,"given":"Allison","family":"Kraus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"key":"3595_CR1","doi-asserted-by":"publisher","first-page":"17565","DOI":"10.1038\/s41598-017-17770-8","volume":"7","author":"BA Abdulrahman","year":"2017","unstructured":"Abdulrahman BA, Abdelaziz D, Thapa S, Lu L, Jain S, Gilch S, Proniuk S, Zukiwski A, Schatzl HM (2017) The celecoxib derivatives AR-12 and AR-14 induce autophagy and clear prion-infected cells from prions. Sci Rep 7:17565","journal-title":"Sci Rep"},{"key":"3595_CR2","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1016\/j.bbrc.2006.10.093","volume":"351","author":"T Arai","year":"2006","unstructured":"Arai T, Hasegawa M, Akiyama H, Ikeda K, Nonaka T, Mori H, Mann D, Tsuchiya K, Yoshida M, Hashizume Y, Oda T (2006) TDP-43 is a component of ubiquitin-positive tau-negative inclusions in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Biochem Biophys Res Commun 351:602\u2013611","journal-title":"Biochem Biophys Res Commun"},{"key":"3595_CR3","unstructured":"Alzheimer's Association (2021) Alzheimer's Disease Facts and Figures. Alzheimers Dement 2021:17(3)"},{"key":"3595_CR4","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1038\/nmeth1066","volume":"4","author":"R Atarashi","year":"2007","unstructured":"Atarashi R, Moore RA, Sim VL, Hughson AG, Dorward DW, Onwubiko HA, Priola SA, Caughey B (2007) Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein. Nat Methods 4:645\u2013650","journal-title":"Nat Methods"},{"key":"3595_CR5","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1038\/nm.2294","volume":"17","author":"R Atarashi","year":"2011","unstructured":"Atarashi R, Satoh K, Sano K, Fuse T, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Yamanaka H, Shirabe S, Yamada M, Mizusawa H, Kitamoto T, Klug G, McGlade A, Collins SJ, Nishida N (2011) Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. Nat Med 17:175\u2013178","journal-title":"Nat Med"},{"key":"3595_CR6","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1111\/nan.12303","volume":"42","author":"RA Barker","year":"2016","unstructured":"Barker RA, Williams-Gray CH (2016) Review: the spectrum of clinical features seen with alpha synuclein pathology. Neuropathol Appl Neurobiol 42:6\u201319","journal-title":"Neuropathol Appl Neurobiol"},{"key":"3595_CR7","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1097\/NEN.0b013e31824b211b","volume":"71","author":"TG Beach","year":"2012","unstructured":"Beach TG, Monsell SE, Phillips LE, Kukull W (2012) Accuracy of the clinical diagnosis of Alzheimer disease at National Institute on Aging Alzheimer Disease Centers, 2005\u20132010. J Neuropathol Exp Neurol 71:266\u2013273","journal-title":"J Neuropathol Exp Neurol"},{"key":"3595_CR8","doi-asserted-by":"publisher","first-page":"698","DOI":"10.1038\/375698a0","volume":"375","author":"RA Bessen","year":"1995","unstructured":"Bessen RA, Kocisko DA, Raymond GJ, Nandan S, Lansbury PT, Caughey B (1995) Non-genetic propagation of strain-specific properties of scrapie prion protein. Nature 375:698\u2013700","journal-title":"Nature"},{"key":"3595_CR9","doi-asserted-by":"publisher","first-page":"2096","DOI":"10.1128\/jvi.66.4.2096-2101.1992","volume":"66","author":"RA Bessen","year":"1992","unstructured":"Bessen RA, Marsh RF (1992) Biochemical and physical properties of the prion protein from two strains of the transmissible mink encephalopathy agent. J Virol 66:2096\u20132101","journal-title":"J Virol"},{"key":"3595_CR10","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1016\/j.bbapap.2010.04.001","volume":"1804","author":"M Biancalana","year":"2010","unstructured":"Biancalana M, Koide S (2010) Molecular mechanism of thioflavin-T binding to amyloid fibrils. Biochim Biophys Acta 1804:1405\u20131412","journal-title":"Biochim Biophys Acta"},{"key":"3595_CR11","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1001\/jamaneurol.2016.4614","volume":"74","author":"M Bongianni","year":"2017","unstructured":"Bongianni M, Orru C, Groveman BR, Sacchetto L, Fiorini M, Tonoli G, Triva G, Capaldi S, Testi S, Ferrari S, Cagnin A, Ladogana A, Poleggi A, Colaizzo E, Tiple D, Vaianella L, Castriciano S, Marchioni D, Hughson AG, Imperiale D, Cattaruzza T, Fabrizi GM, Pocchiari M, Monaco S, Caughey B, Zanusso G (2017) Diagnosis of human prion disease using real-time quaking-induced conversion testing of olfactory mucosa and cerebrospinal fluid samples. JAMA Neurol 74:155\u2013162","journal-title":"JAMA Neurol"},{"key":"3595_CR12","doi-asserted-by":"publisher","first-page":"108843","DOI":"10.1016\/j.celrep.2021.108843","volume":"34","author":"Y Carlomagno","year":"2021","unstructured":"Carlomagno Y, Manne S, DeTure M, Prudencio M, Zhang YJ, Hanna Al-Shaikh R, Dunmore JA, Daughrity LM, Song Y, Castanedes-Casey M, Lewis-Tuffin LJ, Nicholson KA, Wszolek ZK, Dickson DW, Fitzpatrick AWP, Petrucelli L, Cook CN (2021) The AD tau core spontaneously self-assembles and recruits full-length tau to filaments. Cell Rep 34:108843","journal-title":"Cell Rep"},{"key":"3595_CR13","doi-asserted-by":"publisher","first-page":"2557","DOI":"10.1038\/emboj.2008.181","volume":"27","author":"J Castilla","year":"2008","unstructured":"Castilla J, Morales R, Saa P, Barria M, Gambetti P, Soto C (2008) Cell-free propagation of prion strains. EMBO J 27:2557\u20132566","journal-title":"EMBO J"},{"key":"3595_CR14","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.cell.2005.02.011","volume":"121","author":"J Castilla","year":"2005","unstructured":"Castilla J, Saa P, Hetz C, Soto C (2005) In vitro generation of infectious scrapie prions. Cell 121:195\u2013206","journal-title":"Cell"},{"key":"3595_CR15","doi-asserted-by":"crossref","unstructured":"Concha-Marambio L, Farris CM, Holguin B, Ma Y, Seibyl J, Russo MJ, Kang UJ, Hutten SJ, Merchant K, Shahnawaz M, Soto C (2021) Seed amplification assay to diagnose early Parkinson's and predict dopaminergic deficit progression. Mov Disord 36:2444\u20132446","DOI":"10.1002\/mds.28715"},{"key":"3595_CR16","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1186\/s40035-019-0164-x","volume":"8","author":"CMG De Luca","year":"2019","unstructured":"De Luca CMG, Elia AE, Portaleone SM, Cazzaniga FA, Rossi M, Bistaffa E, De Cecco E, Narkiewicz J, Salzano G, Carletta O, Romito L, Devigili G, Soliveri P, Tiraboschi P, Legname G, Tagliavini F, Eleopra R, Giaccone G, Moda F (2019) Efficient RT-QuIC seeding activity for alpha-synuclein in olfactory mucosa samples of patients with Parkinson\u2019s disease and multiple system atrophy. Transl Neurodegener 8:24","journal-title":"Transl Neurodegener"},{"key":"3595_CR17","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1002\/acn3.338","volume":"3","author":"G Fairfoul","year":"2016","unstructured":"Fairfoul G, McGuire LI, Pal S, Ironside JW, Neumann J, Christie S, Joachim C, Esiri M, Evetts SG, Rolinski M, Baig F, Ruffmann C, Wade-Martins R, Hu MT, Parkkinen L, Green AJ (2016) Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies. Ann Clin Transl Neurol 3:812\u2013818","journal-title":"Ann Clin Transl Neurol"},{"key":"3595_CR18","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1038\/s41586-018-0454-y","volume":"561","author":"B Falcon","year":"2018","unstructured":"Falcon B, Zhang W, Murzin AG, Murshudov G, Garringer HJ, Vidal R, Crowther RA, Ghetti B, Scheres SHW, Goedert M (2018a) Structures of filaments from Pick\u2019s disease reveal a novel tau protein fold. Nature 561:137\u2013140","journal-title":"Nature"},{"key":"3595_CR19","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1007\/s00401-018-1914-z","volume":"136","author":"B Falcon","year":"2018","unstructured":"Falcon B, Zhang W, Schweighauser M, Murzin AG, Vidal R, Garringer HJ, Ghetti B, Scheres SHW, Goedert M (2018b) Tau filaments from multiple cases of sporadic and inherited Alzheimer\u2019s disease adopt a common fold. Acta Neuropathol 136:699\u2013708","journal-title":"Acta Neuropathol"},{"key":"3595_CR20","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1038\/s41586-019-1026-5","volume":"568","author":"B Falcon","year":"2019","unstructured":"Falcon B, Zivanov J, Zhang W, Murzin AG, Garringer HJ, Vidal R, Crowther RA, Newell KL, Ghetti B, Goedert M, Scheres SHW (2019) Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules. Nature 568:420\u2013423","journal-title":"Nature"},{"key":"3595_CR21","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1038\/nature23002","volume":"547","author":"AWP Fitzpatrick","year":"2017","unstructured":"Fitzpatrick AWP, Falcon B, He S, Murzin AG, Murshudov G, Garringer HJ, Crowther RA, Ghetti B, Goedert M, Scheres SHW (2017) Cryo-EM structures of tau filaments from Alzheimer\u2019s disease. Nature 547:185\u2013190","journal-title":"Nature"},{"key":"3595_CR22","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1002\/ana.24833","volume":"81","author":"A Foutz","year":"2017","unstructured":"Foutz A, Appleby BS, Hamlin C, Liu X, Yang S, Cohen Y, Chen W, Blevins J, Fausett C, Wang H, Gambetti P, Zhang S, Hughson A, Tatsuoka C, Schonberger LB, Cohen ML, Caughey B, Safar JG (2017) Diagnostic and prognostic value of human prion detection in cerebrospinal fluid. Ann Neurol 81:79\u201392","journal-title":"Ann Neurol"},{"key":"3595_CR23","doi-asserted-by":"publisher","first-page":"10655","DOI":"10.1038\/s41598-017-10922-w","volume":"7","author":"A Franceschini","year":"2017","unstructured":"Franceschini A, Baiardi S, Hughson AG, McKenzie N, Moda F, Rossi M, Capellari S, Green A, Giaccone G, Caughey B, Parchi P (2017) High diagnostic value of second generation CSF RT-QuIC across the wide spectrum of CJD prions. Sci Rep 7:10655","journal-title":"Sci Rep"},{"key":"3595_CR24","doi-asserted-by":"publisher","first-page":"1024","DOI":"10.1002\/acn3.772","volume":"6","author":"A Garrido","year":"2019","unstructured":"Garrido A, Fairfoul G, Tolosa ES, Marti MJ, Green A, Barcelona LSG (2019) alpha-synuclein RT-QuIC in cerebrospinal fluid of LRRK2-linked Parkinson\u2019s disease. Ann Clin Transl Neurol 6:1024\u20131032","journal-title":"Ann Clin Transl Neurol"},{"key":"3595_CR25","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1146\/annurev-neuro-072116-031153","volume":"40","author":"M Goedert","year":"2017","unstructured":"Goedert M, Eisenberg DS, Crowther RA (2017) Propagation of tau aggregates and neurodegeneration. Annu Rev Neurosci 40:189\u2013210","journal-title":"Annu Rev Neurosci"},{"key":"3595_CR26","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1136\/practneurol-2018-001935","volume":"19","author":"AJE Green","year":"2019","unstructured":"Green AJE (2019) RT-QuIC: a new test for sporadic CJD. Pract Neurol 19:49\u201355","journal-title":"Pract Neurol"},{"key":"3595_CR27","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1074\/jbc.M114.619627","volume":"290","author":"BR Groveman","year":"2015","unstructured":"Groveman BR, Kraus A, Raymond LD, Dolan MA, Anson KJ, Dorward DW, Caughey B (2015) Charge neutralization of the central lysine cluster in prion protein (PrP) promotes PrP(Sc)-like folding of recombinant PrP amyloids. J Biol Chem 290:1119\u20131128","journal-title":"J Biol Chem"},{"key":"3595_CR28","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s40478-018-0508-2","volume":"6","author":"BR Groveman","year":"2018","unstructured":"Groveman BR, Orru CD, Hughson AG, Raymond LD, Zanusso G, Ghetti B, Campbell KJ, Safar J, Galasko D, Caughey B (2018) Rapid and ultra-sensitive quantitation of disease-associated alpha-synuclein seeds in brain and cerebrospinal fluid by alphaSyn RT-QuIC. Acta Neuropathol Commun 6:7","journal-title":"Acta Neuropathol Commun"},{"key":"3595_CR29","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1111\/j.1750-3639.2000.tb00269.x","volume":"10","author":"RL Hamilton","year":"2000","unstructured":"Hamilton RL (2000) Lewy bodies in Alzheimer\u2019s disease: a neuropathological review of 145 cases using alpha-synuclein immunohistochemistry. Brain Pathol 10:378\u2013384","journal-title":"Brain Pathol"},{"key":"3595_CR30","doi-asserted-by":"crossref","unstructured":"Hanseeuw BJ, Betensky RA, Jacobs HIL, Schultz AP, Sepulcre J, Becker AJ, Cosio DMO, Farrell M, Quiroz YT, Mormino EC, Buckley RF, Papp KV, Amariglio RA, Dewachter I, Ivanoiu A, Huijbers W, Hedden T, Marshall GA, Chhatwal JP, Rentz DM, Sperling RA, Johnson K (2019) Association of amyloid and tau with cognition in preclinical Alzheimer disease. JAMA Neurol 76","DOI":"10.1001\/jamaneurol.2019.1424"},{"key":"3595_CR31","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1038\/s41591-021-01382-x","volume":"27","author":"O Hansson","year":"2021","unstructured":"Hansson O (2021) Biomarkers for neurodegenerative diseases. Nat Med 27:954\u2013963","journal-title":"Nat Med"},{"key":"3595_CR32","doi-asserted-by":"publisher","first-page":"e1005914","DOI":"10.1371\/journal.ppat.1005914","volume":"12","author":"AG Hughson","year":"2016","unstructured":"Hughson AG, Race B, Kraus A, Sangare LR, Robins L, Groveman BR, Saijo E, Phillips K, Contreras L, Dhaliwal V, Manca M, Zanusso G, Terry D, Williams JF, Caughey B (2016) Inactivation of prions and amyloid seeds with hypochlorous acid. PLoS Pathog 12:e1005914","journal-title":"PLoS Pathog"},{"key":"3595_CR33","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1038\/370471a0","volume":"370","author":"DA Kocisko","year":"1994","unstructured":"Kocisko DA, Come JH, Priola SA, Chesebro B, Raymond GJ, Lansbury PT, Caughey B (1994) Cell-free formation of protease-resistant prion protein. Nature 370:471\u2013474","journal-title":"Nature"},{"key":"3595_CR34","doi-asserted-by":"publisher","first-page":"43155","DOI":"10.1038\/srep43155","volume":"7","author":"N Kondru","year":"2017","unstructured":"Kondru N, Manne S, Greenlee J, West Greenlee H, Anantharam V, Halbur P, Kanthasamy A, Kanthasamy A (2017) Integrated Organotypic Slice Cultures and RT-QuIC (OSCAR) Assay: implications for translational discovery in protein misfolding diseases. Sci Rep 7:43155","journal-title":"Sci Rep"},{"key":"3595_CR35","doi-asserted-by":"publisher","first-page":"21510","DOI":"10.1074\/jbc.M115.665844","volume":"290","author":"A Kraus","year":"2015","unstructured":"Kraus A, Anson KJ, Raymond LD, Martens C, Groveman BR, Dorward DW, Caughey B (2015) Prion protein prolines 102 and 105 and the surrounding lysine cluster impede amyloid formation. J Biol Chem 290:21510\u201321522","journal-title":"J Biol Chem"},{"key":"3595_CR36","doi-asserted-by":"crossref","unstructured":"Kraus A, Hoyt F, Schwartz CL, Hansen B, Artikis E, Hughson AG, Raymond GJ, Race B, Baron GS, Caughey B (2021) High-resolution structure and strain comparison of infectious mammalian prions. Mol Cell 81:4540\u20134551","DOI":"10.1016\/j.molcel.2021.08.011"},{"key":"3595_CR37","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1007\/s00401-018-1947-3","volume":"137","author":"A Kraus","year":"2019","unstructured":"Kraus A, Saijo E, Metrick MA 2nd, Newell K, Sigurdson CJ, Zanusso G, Ghetti B, Caughey B (2019) Seeding selectivity and ultrasensitive detection of tau aggregate conformers of Alzheimer disease. Acta Neuropathol 137:585\u2013598","journal-title":"Acta Neuropathol"},{"key":"3595_CR38","doi-asserted-by":"publisher","first-page":"2069","DOI":"10.1212\/01.WNL.0000165987.89198.65","volume":"64","author":"ML Kraybill","year":"2005","unstructured":"Kraybill ML, Larson EB, Tsuang DW, Teri L, McCormick WC, Bowen JD, Kukull WA, Leverenz JB, Cherrier MM (2005) Cognitive differences in dementia patients with autopsy-verified AD, Lewy body pathology, or both. Neurology 64:2069\u20132073","journal-title":"Neurology"},{"key":"3595_CR39","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1038\/s41593-019-0541-x","volume":"23","author":"A Lau","year":"2020","unstructured":"Lau A, So RWL, Lau HHC, Sang JC, Ruiz-Riquelme A, Fleck SC, Stuart E, Menon S, Visanji NP, Meisl G, Faidi R, Marano MM, Schmitt-Ulms C, Wang Z, Fraser PE, Tandon A, Hyman BT, Wille H, Ingelsson M, Klenerman D, Watts JC (2020) alpha-Synuclein strains target distinct brain regions and cell types. Nat Neurosci 23:21\u201331","journal-title":"Nat Neurosci"},{"key":"3595_CR40","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1038\/s41380-018-0342-8","volume":"24","author":"A Leuzy","year":"2019","unstructured":"Leuzy A, Chiotis K, Lemoine L, Gillberg PG, Almkvist O, Rodriguez-Vieitez E, Nordberg A (2019) Tau PET imaging in neurodegenerative tauopathies-still a challenge. Mol Psychiatry 24:1112\u20131134","journal-title":"Mol Psychiatry"},{"key":"3595_CR41","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1001\/archneur.63.3.370","volume":"63","author":"JB Leverenz","year":"2006","unstructured":"Leverenz JB, Fishel MA, Peskind ER, Montine TJ, Nochlin D, Steinbart E, Raskind MA, Schellenberg GD, Bird TD, Tsuang D (2006) Lewy body pathology in familial Alzheimer disease: evidence for disease- and mutation-specific pathologic phenotype. Arch Neurol 63:370\u2013376","journal-title":"Arch Neurol"},{"key":"3595_CR42","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1016\/j.neuron.2013.07.033","volume":"79","author":"SC Ling","year":"2013","unstructured":"Ling SC, Polymenidou M, Cleveland DW (2013) Converging mechanisms in ALS and FTD: disrupted RNA and protein homeostasis. Neuron 79:416\u2013438","journal-title":"Neuron"},{"key":"3595_CR43","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1002\/1531-8249(199903)45:3<353::AID-ANA11>3.0.CO;2-4","volume":"45","author":"CF Lippa","year":"1999","unstructured":"Lippa CF, Schmidt ML, Lee VM, Trojanowski JQ (1999) Antibodies to alpha-synuclein detect Lewy bodies in many Down\u2019s syndrome brains with Alzheimer\u2019s disease. Ann Neurol 45:353\u2013357","journal-title":"Ann Neurol"},{"key":"3595_CR44","doi-asserted-by":"crossref","unstructured":"Mammana A, Baiardi S, Quadalti C, Rossi M, Donadio V, Capellari S, Liguori R, Parchi P (2021) RT-QuIC Detection of pathological alpha-synuclein in skin punches of patients with Lewy Body disease. Mov Disord 36:2173\u20132177","DOI":"10.1002\/mds.28651"},{"key":"3595_CR45","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1002\/mds.27907","volume":"35","author":"S Manne","year":"2020","unstructured":"Manne S, Kondru N, Jin H, Anantharam V, Huang X, Kanthasamy A, Kanthasamy AG (2020a) alpha-Synuclein real-time quaking-induced conversion in the submandibular glands of Parkinson\u2019s disease patients. Mov Disord 35:268\u2013278","journal-title":"Mov Disord"},{"key":"3595_CR46","doi-asserted-by":"publisher","first-page":"2230","DOI":"10.1002\/mds.28242","volume":"35","author":"S Manne","year":"2020","unstructured":"Manne S, Kondru N, Jin H, Serrano GE, Anantharam V, Kanthasamy A, Adler CH, Beach TG, Kanthasamy AG (2020b) Blinded RT-QuIC analysis of alpha-synuclein biomarker in skin tissue from Parkinson\u2019s disease patients. Mov Disord 35:2230\u20132239","journal-title":"Mov Disord"},{"key":"3595_CR47","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/s40478-020-0887-z","volume":"8","author":"MA Metrick 2nd","year":"2020","unstructured":"Metrick MA 2nd, Ferreira NDC, Saijo E, Kraus A, Newell K, Zanusso G, Vendruscolo M, Ghetti B, Caughey B (2020) A single ultrasensitive assay for detection and discrimination of tau aggregates of Alzheimer and Pick diseases. Acta Neuropathol Commun 8:22","journal-title":"Acta Neuropathol Commun"},{"key":"3595_CR48","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/1750-1326-9-43","volume":"9","author":"S Moussaud","year":"2014","unstructured":"Moussaud S, Jones DR, Moussaud-Lamodiere EL, Delenclos M, Ross OA, McLean PJ (2014) Alpha-synuclein and tau: teammates in neurodegeneration? Mol Neurodegener 9:43","journal-title":"Mol Neurodegener"},{"key":"3595_CR49","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1126\/science.1134108","volume":"314","author":"M Neumann","year":"2006","unstructured":"Neumann M, Sampathu DM, Kwong LK, Truax AC, Micsenyi MC, Chou TT, Bruce J, Schuck T, Grossman M, Clark CM, McCluskey LF, Miller BL, Masliah E, Mackenzie IR, Feldman H, Feiden W, Kretzschmar HA, Trojanowski JQ, Lee VM (2006) Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science 314:130\u2013133","journal-title":"Science"},{"key":"3595_CR50","first-page":"764","volume":"140","author":"M Ono","year":"2017","unstructured":"Ono M, Sahara N, Kumata K, Ji B, Ni R, Koga S, Dickson DW, Trojanowski JQ, Lee VM, Yoshida M, Hozumi I, Yoshiyama Y, van Swieten JC, Nordberg A, Suhara T, Zhang MR, Higuchi M (2017) Distinct binding of PET ligands PBB3 and AV-1451 to tau fibril strains in neurodegenerative tauopathies. Brain 140:764\u2013780","journal-title":"Brain"},{"key":"3595_CR51","doi-asserted-by":"publisher","first-page":"e1004983","DOI":"10.1371\/journal.ppat.1004983","volume":"11","author":"CD Orru","year":"2015","unstructured":"Orru CD, Groveman BR, Raymond LD, Hughson AG, Nonno R, Zou W, Ghetti B, Gambetti P, Caughey B (2015) Bank vole prion protein as an apparently universal substrate for RT-QuIC-based detection and discrimination of prion strains. PLoS Pathog 11:e1004983","journal-title":"PLoS Pathog"},{"key":"3595_CR52","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1038\/s41586-018-0104-4","volume":"557","author":"C Peng","year":"2018","unstructured":"Peng C, Gathagan RJ, Covell DJ, Medellin C, Stieber A, Robinson JL, Zhang B, Pitkin RM, Olufemi MF, Luk KC, Trojanowski JQ, Lee VM (2018) Cellular milieu imparts distinct pathological alpha-synuclein strains in alpha-synucleinopathies. Nature 557:558\u2013563","journal-title":"Nature"},{"key":"3595_CR53","doi-asserted-by":"publisher","first-page":"fcab045","DOI":"10.1093\/braincomms\/fcab045","volume":"3","author":"D Perra","year":"2021","unstructured":"Perra D, Bongianni M, Novi G, Janes F, Bessi V, Capaldi S, Sacchetto L, Tagliapietra M, Schenone G, Morbelli S, Fiorini M, Cattaruzza T, Mazzon G, Orru CD, Catalan M, Polverino P, Bernardini A, Pellitteri G, Valente M, Bertolotti C, Nacmias B, Maggiore G, Cavallaro T, Manganotti P, Gigli G, Monaco S, Nobili F, Zanusso G (2021) Alpha-synuclein seeds in olfactory mucosa and cerebrospinal fluid of patients with dementia with Lewy bodies. Brain Commun 3:fcab045","journal-title":"Brain Commun"},{"key":"3595_CR54","doi-asserted-by":"publisher","first-page":"e1017","DOI":"10.1212\/WNL.0000000000010086","volume":"95","author":"DD Rhoads","year":"2020","unstructured":"Rhoads DD, Wrona A, Foutz A, Blevins J, Glisic K, Person M, Maddox RA, Belay ED, Schonberger LB, Tatsuoka C, Cohen ML, Appleby BS (2020) Diagnosis of prion diseases by RT-QuIC results in improved surveillance. Neurology 95:e1017\u2013e1026","journal-title":"Neurology"},{"key":"3595_CR55","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1212\/WNL.0000000000002350","volume":"86","author":"G Rizzo","year":"2016","unstructured":"Rizzo G, Copetti M, Arcuti S, Martino D, Fontana A, Logroscino G (2016) Accuracy of clinical diagnosis of Parkinson disease: a systematic review and meta-analysis. Neurology 86:566\u2013576","journal-title":"Neurology"},{"key":"3595_CR56","doi-asserted-by":"crossref","unstructured":"Rossi M, Candelise N, Baiardi S, Capellari S, Giannini G, Orru CD, Antelmi E, Mammana A, Hughson AG, Calandra-Buonaura G, Ladogana A, Plazzi G, Cortelli P, Caughey B, Parchi P (2020) Ultrasensitive RT-QuIC assay with high sensitivity and specificity for Lewy body-associated synucleinopathies. Acta Neuropathol","DOI":"10.1007\/s00401-020-02160-8"},{"key":"3595_CR57","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1038\/35081095","volume":"411","author":"GP Saborio","year":"2001","unstructured":"Saborio GP, Permanne B, Soto C (2001) Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding. Nature 411:810\u2013813","journal-title":"Nature"},{"key":"3595_CR58","doi-asserted-by":"publisher","first-page":"1157","DOI":"10.1038\/2654","volume":"4","author":"J Safar","year":"1998","unstructured":"Safar J, Wille H, Itri V, Groth D, Serban H, Torchia M, Cohen FE, Prusiner SB (1998) Eight prion strains have PrP(Sc) molecules with different conformations. Nat Med 4:1157\u20131165","journal-title":"Nat Med"},{"key":"3595_CR59","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1007\/s00401-017-1692-z","volume":"133","author":"E Saijo","year":"2017","unstructured":"Saijo E, Ghetti B, Zanusso G, Oblak A, Furman JL, Diamond MI, Kraus A, Caughey B (2017) Ultrasensitive and selective detection of 3-repeat tau seeding activity in Pick disease brain and cerebrospinal fluid. Acta Neuropathol 133:751\u2013765","journal-title":"Acta Neuropathol"},{"key":"3595_CR60","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1007\/s00401-019-02080-2","volume":"139","author":"E Saijo","year":"2020","unstructured":"Saijo E, Metrick MA 2nd, Koga S, Parchi P, Litvan I, Spina S, Boxer A, Rojas JC, Galasko D, Kraus A, Rossi M, Newell K, Zanusso G, Grinberg LT, Seeley WW, Ghetti B, Dickson DW, Caughey B (2020) 4-Repeat tau seeds and templating subtypes as brain and CSF biomarkers of frontotemporal lobar degeneration. Acta Neuropathol 139:63\u201377","journal-title":"Acta Neuropathol"},{"key":"3595_CR61","first-page":"342","volume":"26","author":"S Sakarya","year":"2014","unstructured":"Sakarya S, Gunay N, Karakulak M, Ozturk B, Ertugrul B (2014) Hypochlorous Acid: an ideal wound care agent with powerful microbicidal, antibiofilm, and wound healing potency. Wounds 26:342\u2013350","journal-title":"Wounds"},{"key":"3595_CR62","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.celrep.2014.02.031","volume":"7","author":"N Salvadores","year":"2014","unstructured":"Salvadores N, Shahnawaz M, Scarpini E, Tagliavini F, Soto C (2014) Detection of misfolded Abeta oligomers for sensitive biochemical diagnosis of Alzheimer\u2019s disease. Cell Rep 7:261\u2013268","journal-title":"Cell Rep"},{"key":"3595_CR63","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1038\/nrn.2017.91","volume":"18","author":"AHV Schapira","year":"2017","unstructured":"Schapira AHV, Chaudhuri KR, Jenner P (2017) Non-motor features of Parkinson disease. Nat Rev Neurosci 18:509","journal-title":"Nat Rev Neurosci"},{"key":"3595_CR64","doi-asserted-by":"crossref","unstructured":"Schweighauser M, Shi Y, Tarutani A, Kametani F, Murzin AG, Ghetti B, Matsubara T, Tomita T, Ando T, Hasegawa K, Murayama S, Yoshida M, Hasegawa M, Scheres SHW, Goedert M (2020) Structures of alpha-synuclein filaments from multiple system atrophy. Nature","DOI":"10.1101\/2020.02.05.935619"},{"key":"3595_CR65","doi-asserted-by":"publisher","first-page":"fcaa142","DOI":"10.1093\/braincomms\/fcaa142","volume":"2","author":"C Scialo","year":"2020","unstructured":"Scialo C, Tran TH, Salzano G, Novi G, Caponnetto C, Chio A, Calvo A, Canosa A, Moda F, Caroppo P, Silani V, Ticozzi N, Ratti A, Borroni B, Benussi L, Ghidoni R, Furlanis G, Manganotti P, Senigagliesi B, Parisse P, Brasselet R, Buratti E, Legname G (2020) TDP-43 real-time quaking induced conversion reaction optimization and detection of seeding activity in CSF of amyotrophic lateral sclerosis and frontotemporal dementia patients. Brain Commun 2:fcaa142","journal-title":"Brain Commun"},{"key":"3595_CR66","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1038\/s41586-020-1984-7","volume":"578","author":"M Shahnawaz","year":"2020","unstructured":"Shahnawaz M, Mukherjee A, Pritzkow S, Mendez N, Rabadia P, Liu X, Hu B, Schmeichel A, Singer W, Wu G, Tsai AL, Shirani H, Nilsson KPR, Low PA, Soto C (2020) Discriminating alpha-synuclein strains in Parkinson\u2019s disease and multiple system atrophy. Nature 578:273\u2013277","journal-title":"Nature"},{"key":"3595_CR67","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1001\/jamaneurol.2016.4547","volume":"74","author":"M Shahnawaz","year":"2017","unstructured":"Shahnawaz M, Tokuda T, Waragai M, Mendez N, Ishii R, Trenkwalder C, Mollenhauer B, Soto C (2017) Development of a biochemical diagnosis of Parkinson disease by detection of alpha-synuclein misfolded aggregates in cerebrospinal fluid. JAMA Neurol 74:163\u2013172","journal-title":"JAMA Neurol"},{"key":"3595_CR68","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1007\/s00401-021-02294-3","volume":"141","author":"Y Shi","year":"2021","unstructured":"Shi Y, Murzin AG, Falcon B, Epstein A, Machin J, Tempest P, Newell KL, Vidal R, Garringer HJ, Sahara N, Higuchi M, Ghetti B, Jang MK, Scheres SHW, Goedert M (2021a) Cryo-EM structures of tau filaments from Alzheimer\u2019s disease with PET ligand APN-1607. Acta Neuropathol 141:697\u2013708","journal-title":"Acta Neuropathol"},{"key":"3595_CR69","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1038\/s41586-021-03911-7","volume":"598","author":"Y Shi","year":"2021","unstructured":"Shi Y, Zhang W, Yang Y, Murzin AG, Falcon B, Kotecha A, van Beers M, Tarutani A, Kametani F, Garringer HJ, Vidal R, Hallinan GI, Lashley T, Saito Y, Murayama S, Yoshida M, Tanaka H, Kakita A, Ikeuchi T, Robinson AC, Mann DMA, Kovacs GG, Revesz T, Ghetti B, Hasegawa M, Goedert M, Scheres SHW (2021b) Structure-based classification of tauopathies. Nature 598:359\u2013363","journal-title":"Nature"},{"key":"3595_CR70","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1007\/s00401-021-02288-1","volume":"141","author":"A Sokratian","year":"2021","unstructured":"Sokratian A, Ziaee J, Kelly K, Chang A, Bryant N, Wang S, Xu E, Li JY, Wang SH, Ervin J, Swain SM, Liddle RA, West AB (2021) Heterogeneity in alpha-synuclein fibril activity correlates to disease phenotypes in Lewy body dementia. Acta Neuropathol 141:547\u2013564","journal-title":"Acta Neuropathol"},{"key":"3595_CR71","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1093\/brain\/awab005","volume":"144","author":"A Stefani","year":"2021","unstructured":"Stefani A, Iranzo A, Holzknecht E, Perra D, Bongianni M, Gaig C, Heim B, Serradell M, Sacchetto L, Garrido A, Capaldi S, Sanchez-Gomez A, Cecchini MP, Mariotto S, Ferrari S, Fiorini M, Schmutzhard J, Cocchiara P, Vilaseca I, Brozzetti L, Monaco S, Jose Marti M, Seppi K, Tolosa E, Santamaria J, Hogl B, Poewe W, Zanusso G, S. group (2021) Alpha-synuclein seeds in olfactory mucosa of patients with isolated REM sleep behaviour disorder. Brain 144:1118\u20131126","journal-title":"Brain"},{"key":"3595_CR72","doi-asserted-by":"publisher","first-page":"2079","DOI":"10.1126\/science.274.5295.2079","volume":"274","author":"GC Telling","year":"1996","unstructured":"Telling GC, Parchi P, DeArmond SJ, Cortelli P, Montagna P, Gabizon R, Mastrianni J, Lugaresi E, Gambetti P, Prusiner SB (1996) Evidence for the conformation of the pathologic isoform of the prion protein enciphering and propagating prion diversity. Science 274:2079\u20132082","journal-title":"Science"},{"key":"3595_CR73","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1080\/19336896.2020.1832946","volume":"14","author":"JM Tennant","year":"2020","unstructured":"Tennant JM, Henderson DM, Wisniewski TM, Hoover EA (2020) RT-QuIC detection of tauopathies using full-length tau substrates. Prion 14:249\u2013256","journal-title":"Prion"},{"key":"3595_CR74","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1002\/ana.25447","volume":"85","author":"A van Rumund","year":"2019","unstructured":"van Rumund A, Green AJE, Fairfoul G, Esselink RAJ, Bloem BR, Verbeek MM (2019) alpha-Synuclein real-time quaking-induced conversion in the cerebrospinal fluid of uncertain cases of parkinsonism. Ann Neurol 85:777\u2013781","journal-title":"Ann Neurol"},{"key":"3595_CR75","doi-asserted-by":"crossref","unstructured":"Villemagne VL, Burnham S, Bourgeat P, Brown B, Ellis KA, Salvado O, Szoeke C, Macaulay SL, Martins R, Maruff P, Ames D, Rowe CC, Masters CL (2013) Amyloid \u03b2 deposition, neurodegeneration, and cognitive decline in sporadic Alzheimer's disease: a prospective cohort study. Lancet Neurol 12","DOI":"10.1016\/S1474-4422(13)70044-9"},{"key":"3595_CR76","doi-asserted-by":"crossref","unstructured":"Wang Z, Becker K, Donadio V, Siedlak S, Yuan J, Rezaee M, Incensi A, Kuzkina A, Orru CD, Tatsuoka C, Liguori R, Gunzler SA, Caughey B, Jimenez-Capdeville ME, Zhu X, Doppler K, Cui L, Chen SG, Ma J, Zou WQ (2020) Skin alpha-synuclein aggregation seeding activity as a novel biomarker for Parkinson disease. JAMA Neurol","DOI":"10.1001\/jamaneurol.2020.3311"},{"key":"3595_CR77","unstructured":"WHO, W. H. O. (2021) Dementia"},{"key":"3595_CR78","doi-asserted-by":"publisher","first-page":"e1001217","DOI":"10.1371\/journal.ppat.1001217","volume":"6","author":"JM Wilham","year":"2010","unstructured":"Wilham JM, Orru CD, Bessen RA, Atarashi R, Sano K, Race B, Meade-White KD, Taubner LM, Timmes A, Caughey B (2010) Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. PLoS Pathog 6:e1001217","journal-title":"PLoS Pathog"},{"key":"3595_CR79","doi-asserted-by":"publisher","first-page":"e0223659","DOI":"10.1371\/journal.pone.0223659","volume":"14","author":"K Williams","year":"2019","unstructured":"Williams K, Hughson AG, Chesebro B, Race B (2019) Inactivation of chronic wasting disease prions using sodium hypochlorite. PLoS One 14:e0223659","journal-title":"PLoS ONE"},{"key":"3595_CR80","doi-asserted-by":"publisher","first-page":"E4949","DOI":"10.1073\/pnas.1513426112","volume":"112","author":"AL Woerman","year":"2015","unstructured":"Woerman AL, Stohr J, Aoyagi A, Rampersaud R, Krejciova Z, Watts JC, Ohyama T, Patel S, Widjaja K, Oehler A, Sanders DW, Diamond MI, Seeley WW, Middleton LT, Gentleman SM, Mordes DA, Sudhof TC, Giles K, Prusiner SB (2015) Propagation of prions causing synucleinopathies in cultured cells. Proc Natl Acad Sci U S A 112:E4949-4958","journal-title":"Proc Natl Acad Sci U S A"},{"key":"3595_CR81","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1038\/s41586-020-2043-0","volume":"580","author":"W Zhang","year":"2020","unstructured":"Zhang W, Tarutani A, Newell KL, Murzin AG, Matsubara T, Falcon B, Vidal R, Garringer HJ, Shi Y, Ikeuchi T, Murayama S, Ghetti B, Hasegawa M, Goedert M, Scheres SHW (2020) Novel tau filament fold in corticobasal degeneration. Nature 580:283\u2013287","journal-title":"Nature"}],"container-title":["Cell and Tissue Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-022-03595-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00441-022-03595-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-022-03595-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T17:06:45Z","timestamp":1682356005000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00441-022-03595-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,8]]},"references-count":81,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["3595"],"URL":"https:\/\/doi.org\/10.1007\/s00441-022-03595-z","relation":{},"ISSN":["0302-766X","1432-0878"],"issn-type":[{"value":"0302-766X","type":"print"},{"value":"1432-0878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,8]]},"assertion":[{"value":"17 September 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 January 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 March 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"H.S. declares no conflict of interest. A.K. is named as a co-inventor on patent applications related to tau RT-QuIC methodologies.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}