{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T13:47:09Z","timestamp":1775915229641,"version":"3.50.1"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["Prin 2022 - 2022LYPBCT"],"award-info":[{"award-number":["Prin 2022 - 2022LYPBCT"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["Prin 2022 - 2022LYPBCT"],"award-info":[{"award-number":["Prin 2022 - 2022LYPBCT"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["Prin 2022 - 2022LYPBCT"],"award-info":[{"award-number":["Prin 2022 - 2022LYPBCT"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["Prin 2022 - 2022LYPBCT"],"award-info":[{"award-number":["Prin 2022 - 2022LYPBCT"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007195","name":"Universit\u00e0 degli Studi di Napoli Federico II","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007195","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Tissue Res"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Smell and taste are extensively studied in fish species as essential for finding food and selecting mates while avoiding toxic substances and predators. Depending on the evolutionary position and adaptation, a discrete variation in the morphology of these sense organs has been reported in numerous teleost species. Here, for the first time, we approach the phenotypic characterization of the olfactory epithelium and taste buds in the African turquoise killifish (<jats:italic>Nothobranchius furzeri<\/jats:italic>), a model organism known for its short lifespan and use in ageing research. Our observations indicate that the olfactory epithelium of <jats:italic>N. furzeri<\/jats:italic> is organized as a simple patch, lacking the complex folding into a rosette, with an average size of approximately 600\u00a0\u00b5m in length, 300\u00a0\u00b5m in width, and 70\u00a0\u00b5m in thickness. Three main cytotypes, including olfactory receptor neurons (CalbindinD28K), supporting cells (\u03b2-tubulin IV), and basal cells (Ki67), were identified across the epithelium. Further, we determined the taste buds\u2019 distribution and quantification between anterior (skin, lips, oral cavity) and posterior (gills, pharynx, oesophagus) systems. We identified the key cytotypes by using immunohistochemical markers, i.e. CalbindinD28K, doublecortin, and neuropeptide Y (NPY) for gustatory receptor cells, glial fibrillary acidic protein (GFAP) for supporting cells, and Ki67, a marker of cellular proliferation for basal cells. Altogether, these results indicate that <jats:italic>N. furzeri<\/jats:italic> is a microsmatic species with unique taste and olfactory features and possesses a well-developed posterior taste system compared to the anterior. This study provides fundamental insights into the chemosensory biology of <jats:italic>N. furzeri<\/jats:italic>, facilitating future investigations into nutrient-sensing mechanisms and their roles in development, survival, and ageing.<\/jats:p>","DOI":"10.1007\/s00441-024-03923-5","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T04:01:52Z","timestamp":1729483312000},"page":"239-252","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Olfactory and gustatory chemical sensor systems in the African turquoise killifish: Insights from morphology"],"prefix":"10.1007","volume":"398","author":[{"given":"Daniela","family":"Giaquinto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Fonsatti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Bortoletti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Radaelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"De Felice","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"de Girolamo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Bertotto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livia","family":"D\u2019Angelo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,10,21]]},"reference":[{"key":"3923_CR1","doi-asserted-by":"publisher","unstructured":"Ahuja G, Korsching S (2014) Zebrafish olfactory receptor ORA1 recognizes a putative reproductive pheromone. Commun Integr Biol 7(5). https:\/\/doi.org\/10.4161\/19420889.2014.970501","DOI":"10.4161\/19420889.2014.970501"},{"issue":"4","key":"3923_CR2","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1159\/000125438","volume":"4","author":"J Atema","year":"1971","unstructured":"Atema J (1971) Structures and functions of the sense of taste in the catfish (Ictalurus natalis). Brain Behav Evol 4(4):273\u2013294. https:\/\/doi.org\/10.1159\/000125438","journal-title":"Brain Behav Evol"},{"key":"3923_CR3","doi-asserted-by":"publisher","first-page":"270229","DOI":"10.3389\/fnmol.2017.00199","volume":"10","author":"AA Ayanlaja","year":"2017","unstructured":"Ayanlaja AA, Xiong Y, Gao Y, Ji G, Tang C, Abdikani Abdullah Z, Gao D (2017) Distinct features of doublecortin as a marker of neuronal migration and its implications in cancer cell mobility. Front Mol Neurosci 10:270229. https:\/\/doi.org\/10.3389\/fnmol.2017.00199","journal-title":"Front Mol Neurosci"},{"issue":"4","key":"3923_CR4","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1002\/cne.21844","volume":"511","author":"A Barreiro-Iglesias","year":"2008","unstructured":"Barreiro-Iglesias A, Villar-Cervi\u00f1o V, Villar-Cheda B, Anad\u00f3n R, Rodicio MC (2008) Neurochemical characterization of sea lamprey taste buds and afferent gustatory fibers: presence of serotonin, calretinin, and CGRP immunoreactivity in taste bud bi-ciliated cells of the earliest vertebrates. J Comp Neurol 511(4):438\u2013453. https:\/\/doi.org\/10.1002\/cne.21844","journal-title":"J Comp Neurol"},{"issue":"1\u20132","key":"3923_CR5","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/0304-3940(94)90397-2","volume":"169","author":"E Bastianelli","year":"1994","unstructured":"Bastianelli E, Pochet R (1994) Distribution of calmodulin, calbindin-D28k and calretinin among rat olfactory nerve bundles. Neurosci Lett 169(1\u20132):223\u2013226. https:\/\/doi.org\/10.1016\/0304-3940(94)90397-2","journal-title":"Neurosci Lett"},{"issue":"5","key":"3923_CR6","doi-asserted-by":"publisher","first-page":"1764","DOI":"10.1093\/micmic\/ozad099","volume":"29","author":"S Bettini","year":"2023","unstructured":"Bettini S, Lazzari M, Milani L, Maurizii MG, Franceschini V (2023) Immunohistochemical analysis of olfactory sensory neuron populations in the developing olfactory organ of the guppy, Poecilia reticulata (Cyprinodontiformes, Poecilidae). Microsc Microanal 29(5):1764\u20131773. https:\/\/doi.org\/10.1093\/micmic\/ozad099","journal-title":"Microsc Microanal"},{"issue":"2","key":"3923_CR7","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1111\/brv.12183","volume":"91","author":"A Cellerino","year":"2016","unstructured":"Cellerino A, Valenzano DR, Reichard M (2016) From the bush to the bench: the annual Nothobranchius fishes as a new model system in biology. Biol Rev Camb Philos Soc 91(2):511\u2013533. https:\/\/doi.org\/10.1111\/brv.12183","journal-title":"Biol Rev Camb Philos Soc"},{"key":"3923_CR8","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.aanat.2016.02.001","volume":"205","author":"L D\u2019Angelo","year":"2016","unstructured":"D\u2019Angelo L, Lossi L, Merighi A, de Girolamo P (2016) Anatomical features for the adequate choice of experimental animal models in biomedicine: I. Fishes Ann Anat 205:75\u201384. https:\/\/doi.org\/10.1016\/j.aanat.2016.02.001","journal-title":"Fishes Ann Anat"},{"issue":"4","key":"3923_CR9","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1002\/ar.22668","volume":"296","author":"L D'Angelo","year":"2013","unstructured":"D\u2019Angelo L (2013) Brain atlas of an emerging teleostean model: Nothobranchius furzeri. Anat Rec 296(4):681\u2013691. https:\/\/doi.org\/10.1002\/ar.22668","journal-title":"Anat Rec"},{"issue":"2","key":"3923_CR10","doi-asserted-by":"publisher","first-page":"449","DOI":"10.3390\/ani11020449","volume":"11","author":"E De Felice","year":"2021","unstructured":"De Felice E, Giaquinto D, Damiano S, Salzano A, Fabroni S, Ciarcia R, Scocco P, de Girolamo P, D\u2019Angelo L (2021) Distinct pattern of NPY in gastro-entero-pancreatic system of goat kids fed with a new standardized red orange and lemon extract (RLE). Animals 11(2):449. https:\/\/doi.org\/10.3390\/ani11020449","journal-title":"Animals"},{"issue":"suppl. 2","key":"3923_CR11","first-page":"S286","volume":"45","author":"GV Devitsina","year":"2005","unstructured":"Devitsina GV (2005) Comparative morphology of intraoral taste apparatus in fishes. J Ichthyol 45(suppl. 2):S286\u2013S306","journal-title":"J Ichthyol"},{"issue":"3","key":"3923_CR12","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.tice.2012.02.002","volume":"44","author":"EH Elsheikh","year":"2012","unstructured":"Elsheikh EH, Nasr ES, Gamal AM (2012) Ultrastructure and distribution of the taste buds in the buccal cavity in relation to the food and feeding habit of a herbivorous fish: Oreochromis niloticus. Tissue Cell 44(3):164\u2013169. https:\/\/doi.org\/10.1016\/j.tice.2012.02.002","journal-title":"Tissue Cell"},{"key":"3923_CR13","doi-asserted-by":"publisher","first-page":"e49226","DOI":"10.7554\/eLife.49226","volume":"8","author":"D Fan","year":"2019","unstructured":"Fan D, Chettouh Z, Consalez GG, Brunet J-F (2019) Taste bud formation depends on taste nerves. eLife 8:e49226. https:\/\/doi.org\/10.7554\/eLife.49226","journal-title":"eLife"},{"issue":"4688","key":"3923_CR14","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1126\/science.3969566","volume":"227","author":"TE Finger","year":"1985","unstructured":"Finger TE, Morita Y (1985) Two gustatory systems: facial and vagal gustatory nuclei have different brainstem connections. Science 227(4688):776\u2013778. https:\/\/doi.org\/10.1126\/science.3969566","journal-title":"Science"},{"key":"3923_CR15","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/BF01526190","volume":"254","author":"M Fujiwara","year":"1997","unstructured":"Fujiwara M, Nakamura H, Kawasaki M, Nakano Y, Kuwano R (1997) Expressions of a calcium-binding protein (spot35\/calbindin-D28k) in mouse olfactory cells: possible relationship to neuronal differentiation. Eur Arch Otorhinolaryngol 254:105\u2013109. https:\/\/doi.org\/10.1007\/BF01526190","journal-title":"Eur Arch Otorhinolaryngol"},{"issue":"7","key":"3923_CR16","doi-asserted-by":"publisher","first-page":"3839","DOI":"10.3390\/ijms23073839","volume":"23","author":"D Giaquinto","year":"2022","unstructured":"Giaquinto D, De Felice E, Attanasio C, Palladino A, Schiano V, Mollo E, Lucini C, de Girolamo P, D\u2019Angelo L (2022) Central and peripheral NPY age-related regulation: a comparative analysis in fish translational models. Int J Mol Sci 23(7):3839. https:\/\/doi.org\/10.3390\/ijms23073839","journal-title":"Int J Mol Sci"},{"key":"3923_CR17","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1093\/chemse\/23.1.39","volume":"23","author":"A Hansen","year":"1998","unstructured":"Hansen A, Zeiske E (1998) The peripheral olfactory organ of the zebrafish, Danio rerio: an ultrastructural study. Chem Senses 23:39\u201348. https:\/\/doi.org\/10.1093\/chemse\/23.1.39","journal-title":"Chem Senses"},{"key":"3923_CR18","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1111\/j.1095-8649.1994.tb01328.x","volume":"45","author":"TJ Hara","year":"1994","unstructured":"Hara TJ, Kitada Y, Evans RE (1994) Distribution patterns of palatal taste buds and their responses to amino acids in salmonids. J Fish Biol 45:453\u2013465. https:\/\/doi.org\/10.1111\/j.1095-8649.1994.tb01328.x","journal-title":"J Fish Biol"},{"key":"3923_CR19","doi-asserted-by":"publisher","unstructured":"Herness S (2012) The neurobiology of gustation: taste buds and transduction processes. In: Johnson LR, Ghishan FK, Kaunitz JD, Merchant JL, Said HM, Wood JD (eds) Physiology of the gastrointestinal tract, 5th edn. Academic Press, pp 741\u2013767. https:\/\/doi.org\/10.1016\/B978-0-12-382026-6.00026-9","DOI":"10.1016\/B978-0-12-382026-6.00026-9"},{"issue":"8","key":"3923_CR20","doi-asserted-by":"publisher","first-page":"1687","DOI":"10.1002\/lary.21856","volume":"121","author":"EH Holbrook","year":"2011","unstructured":"Holbrook EH, Wu E, Curry WT, Lin DT, Schwob JE (2011) Immunohistochemical characterization of human olfactory tissue. Laryngoscope 121(8):1687\u20131701. https:\/\/doi.org\/10.1002\/lary.21856","journal-title":"Laryngoscope"},{"issue":"1","key":"3923_CR21","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1111\/jfb.13793","volume":"95","author":"Y Hu","year":"2019","unstructured":"Hu Y, Majoris JE, Buston PM, Webb JF (2019) Potential roles of smell and taste in the orientation behaviour of coral-reef fish larvae: insights from morphology. J Fish Biol 95(1):311\u2013323. https:\/\/doi.org\/10.1111\/jfb.13793","journal-title":"J Fish Biol"},{"issue":"3","key":"3923_CR22","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1002\/cne.22097","volume":"516","author":"T Ikenaga","year":"2009","unstructured":"Ikenaga T, Ogura T, Finger TE (2009) Vagal gustatory reflex circuits for intraoral food sorting behavior in the goldfish: cellular organization and neurotransmitters. J Comp Neurol 516(3):213\u2013225. https:\/\/doi.org\/10.1002\/cne.22097","journal-title":"J Comp Neurol"},{"issue":"5","key":"3923_CR23","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1093\/chemse\/bjq019","volume":"35","author":"T Iqbal","year":"2010","unstructured":"Iqbal T, Byrd-Jacobs C (2010) Rapid degeneration and regeneration of the zebrafish olfactory epithelium after triton X-100 application. Chem Senses 35(5):351\u2013361. https:\/\/doi.org\/10.1093\/chemse\/bjq019","journal-title":"Chem Senses"},{"key":"3923_CR24","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.1016\/j.mod.2005.07.005","volume":"122","author":"Y Ishimaru","year":"2005","unstructured":"Ishimaru Y, Okada S, Naito H, Nagai T, Yasuoka A, Matsumoto I, Abe K (2005) Two families of candidate taste receptors in fishes. Mech Dev 122:1310\u20131321. https:\/\/doi.org\/10.1016\/j.mod.2005.07.005","journal-title":"Mech Dev"},{"issue":"4","key":"3923_CR25","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1002\/cne.23470","volume":"522","author":"W Jang","year":"2014","unstructured":"Jang W, Chen X, Flis D, Harris M, Schwob JE (2014) Label-retaining, quiescent globose basal cells are found in the olfactory epithelium. J Comp Neurol 522(4):731\u2013749. https:\/\/doi.org\/10.1002\/cne.23470","journal-title":"J Comp Neurol"},{"issue":"2","key":"3923_CR26","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/S0006-8993(03)02693-3","volume":"977","author":"C Jia","year":"2003","unstructured":"Jia C, Halpern M (2003) Calbindin D28K immunoreactive neurons in vomeronasal organ and their projections to the accessory olfactory bulb in the rat. Brain Res 977(2):261\u2013269. https:\/\/doi.org\/10.1016\/S0006-8993(03)02693-3","journal-title":"Brain Res"},{"key":"3923_CR27","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1111\/jfb.13940","volume":"95","author":"AO Kasumyan","year":"2019","unstructured":"Kasumyan AO (2019) The taste system in fishes and the effects of environmental variables. J Fish Biol 95:155\u2013178. https:\/\/doi.org\/10.1111\/jfb.13940","journal-title":"J Fish Biol"},{"issue":"4","key":"3923_CR28","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1534\/genetics.114.173849","volume":"199","author":"S Kirchmaier","year":"2015","unstructured":"Kirchmaier S, Naruse K, Wittbrodt J, Loosli F (2015) The genomic and genetic toolbox of the teleost medaka (Oryzias latipes). Genetics 199(4):905\u2013918. https:\/\/doi.org\/10.1534\/genetics.114.173849","journal-title":"Genetics"},{"key":"3923_CR29","doi-asserted-by":"publisher","unstructured":"Korsching SI (2020) Taste and smell in zebrafish. In: Fritzsch B (ed) The senses: a comprehensive reference, 2nd edn. Elsevier, pp 466\u2013492. https:\/\/doi.org\/10.1016\/B978-0-12-809324-5.24155-2","DOI":"10.1016\/B978-0-12-809324-5.24155-2"},{"key":"3923_CR30","first-page":"95","volume-title":"Bulletin of the Museum of Comparative Zoology at Harvard College","author":"GV Lauder","year":"1983","unstructured":"Lauder GV, Liem KF (1983) The evolution and interrelationships of the actinopterygian fishes. In: Liem KF (ed) Bulletin of the Museum of Comparative Zoology at Harvard College, vol 150. Cambridge, Mass, The Museum, pp 95\u2013197"},{"issue":"3","key":"3923_CR31","doi-asserted-by":"publisher","first-page":"1778","DOI":"10.3390\/ijms23031778","volume":"23","author":"A Leggieri","year":"2022","unstructured":"Leggieri A, Attanasio C, Palladino A, de Girolamo P, Lucini C, D\u2019Angelo L (2022) Neuronal phenotype of col4a1 and col25a1: an intriguing hypothesis in vertebrates brain aging. Int J Mol Sci 23(3):1778. https:\/\/doi.org\/10.3390\/ijms23031778","journal-title":"Int J Mol Sci"},{"key":"3923_CR32","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.neuroscience.2020.03.026","volume":"434","author":"K Lemons","year":"2020","unstructured":"Lemons K, Fu Z, Ogura T, Lin W (2020) TRPM5-expressing microvillous cells regulate region-specific cell proliferation and apoptosis during chemical exposure. Neuroscience 434:171\u2013190. https:\/\/doi.org\/10.1016\/j.neuroscience.2020.03.026","journal-title":"Neuroscience"},{"key":"3923_CR33","doi-asserted-by":"publisher","first-page":"1326674","DOI":"10.3389\/fnbeh.2023.1326674","volume":"17","author":"V Mari\u00ebn","year":"2024","unstructured":"Mari\u00ebn V, Piskin I, Zandecki C, Vanhoucke J, Arckens L (2024) Age-related alterations in the behavioral response to a novel environment in the African turquoise killifish (Nothobranchius furzeri). Front Behav Neurosci 17:1326674. https:\/\/doi.org\/10.3389\/fnbeh.2023.1326674","journal-title":"Front Behav Neurosci"},{"issue":"6","key":"3923_CR34","doi-asserted-by":"publisher","first-page":"1070","DOI":"10.1002\/jcb.24696","volume":"115","author":"S Mii","year":"2014","unstructured":"Mii S, Amoh Y, Katsuoka K, Hoffman RM (2014) Comparison of nestin-expressing multipotent stem cells in the tongue fungiform papilla and vibrissa hair follicle. J Cell Biochem 115(6):1070\u20131076. https:\/\/doi.org\/10.1002\/jcb.24696","journal-title":"J Cell Biochem"},{"key":"3923_CR35","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1007\/s004410050981","volume":"291","author":"Y Miyawaki","year":"1997","unstructured":"Miyawaki Y, Morisaki I, Tabata M et al (1997) Calbindin D28k-like immunoreactivity in the developing and regenerating circumvallate papilla of the rat. Cell Tissue Res 291:81\u201390. https:\/\/doi.org\/10.1007\/s004410050981","journal-title":"Cell Tissue Res"},{"key":"3923_CR36","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1038\/nrn1365","volume":"5","author":"P Mombaerts","year":"2004","unstructured":"Mombaerts P (2004) Genes and ligands for odorant, vomeronasal and taste receptors. Nat Rev Neurosci 5:263\u2013278. https:\/\/doi.org\/10.1038\/nrn1365","journal-title":"Nat Rev Neurosci"},{"issue":"1","key":"3923_CR37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/cne.20425","volume":"482","author":"RG Northcutt","year":"2005","unstructured":"Northcutt RG (2005) Taste bud development in the channel catfish. J Comp Neurol 482(1):1\u201316. https:\/\/doi.org\/10.1002\/cne.20425","journal-title":"J Comp Neurol"},{"key":"3923_CR38","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1080\/09168451.2015.1023251","volume":"79","author":"S Okada","year":"2015","unstructured":"Okada S (2015) The taste system of small fish species. Biosci Biotechnol Biochem 79:1039\u20131043. https:\/\/doi.org\/10.1080\/09168451.2015.1023251","journal-title":"Biosci Biotechnol Biochem"},{"key":"3923_CR39","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/BF00226604","volume":"153","author":"K Reutter","year":"1974","unstructured":"Reutter K, Breipohl W, Bijvank GJ (1974) Taste bud types in fishes. Cell Tissue Res 153:151\u2013165. https:\/\/doi.org\/10.1007\/BF00226604","journal-title":"Cell Tissue Res"},{"key":"3923_CR40","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.ydbio.2021.03.012","volume":"476","author":"MR Riddle","year":"2021","unstructured":"Riddle MR, Hu CK (2021) Fish models for investigating nutritional regulation of embryonic development. Dev Biol 476:101\u2013111. https:\/\/doi.org\/10.1016\/j.ydbio.2021.03.012","journal-title":"Dev Biol"},{"issue":"2","key":"3923_CR41","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1111\/j.1474-9726.2011.00781.x","volume":"11","author":"ET Terzibasi","year":"2012","unstructured":"Terzibasi ET, Baumgart M, Battistoni G, Cellerino A (2012) Adult neurogenesis in the short-lived teleost Nothobranchius furzeri: localization of neurogenic niches, molecular characterization and effects of aging. Aging Cell 11(2):241\u2013251. https:\/\/doi.org\/10.1111\/j.1474-9726.2011.00781.x","journal-title":"Aging Cell"},{"key":"3923_CR42","doi-asserted-by":"publisher","unstructured":"Thor\u00e9 ESJ, Philippe C, Brendonck L, Pinceel T (2021) Towards improved fish tests in ecotoxicology - efficient chronic and multi-generational testing with the killifish Nothobranchius furzeri. Chemosphere 273. https:\/\/doi.org\/10.1016\/j.chemosphere.2021.129697","DOI":"10.1016\/j.chemosphere.2021.129697"},{"key":"3923_CR43","doi-asserted-by":"publisher","first-page":"8865","DOI":"10.1038\/s41598-021-88317-1","volume":"11","author":"PR Villamayor","year":"2021","unstructured":"Villamayor PR, Arana \u00c1J, Coppel C et al (2021) A comprehensive structural, lectin and immunohistochemical characterization of the zebrafish olfactory system. Sci Rep 11:8865. https:\/\/doi.org\/10.1038\/s41598-021-88317-1","journal-title":"Sci Rep"},{"issue":"3","key":"3923_CR44","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1038\/sj.hdy.6800635","volume":"94","author":"JN Volff","year":"2005","unstructured":"Volff JN (2005) Genome evolution and biodiversity in teleost fish. Heredity 94(3):280\u2013294. https:\/\/doi.org\/10.1038\/sj.hdy.6800635","journal-title":"Heredity"},{"key":"3923_CR45","first-page":"39","volume-title":"Chemoreception in fishes","author":"M Yamamoto","year":"1982","unstructured":"Yamamoto M (1982) Comparative morphology of the peripheral olfactory organ in teleosts. In: Hara TJ (ed) Chemoreception in fishes. Elsevier, New York, pp 39\u201359"},{"key":"3923_CR46","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1093\/oxfordjournals.jbchem.a022528","volume":"126","author":"A Yasuoka","year":"1999","unstructured":"Yasuoka A, Endo K, Asano-Miyoshi M, Abe K, Emori Y (1999) Two subfamilies of olfactory receptor genes in medaka fish, Oryzias latipes: genomic organization and differential expression in olfactory epithelium. J Biochem 126:866\u2013873. https:\/\/doi.org\/10.1093\/oxfordjournals.jbchem.a022528","journal-title":"J Biochem"},{"issue":"3","key":"3923_CR47","doi-asserted-by":"publisher","first-page":"e12774","DOI":"10.1111\/eff.12774","volume":"33","author":"J \u017d\u00e1k","year":"2024","unstructured":"\u017d\u00e1k J, \u0160uhajov\u00e1 P (2024) The effect of water turbidity on prey consumption and female feeding patterns in African turquoise killifish. Ecol Freshw Fish 33(3):e12774. https:\/\/doi.org\/10.1111\/eff.12774","journal-title":"Ecol Freshw Fish"},{"key":"3923_CR48","doi-asserted-by":"publisher","first-page":"8986","DOI":"10.1038\/s41598-020-65930-0","volume":"10","author":"J \u017d\u00e1k","year":"2020","unstructured":"\u017d\u00e1k J, Dykov\u00e1 I, Reichard M (2020) Good performance of turquoise killifish (Nothobranchius furzeri) on pelleted diet as a step towards husbandry standardization. Sci Rep 10:8986. https:\/\/doi.org\/10.1038\/s41598-020-65930-0","journal-title":"Sci Rep"},{"key":"3923_CR49","doi-asserted-by":"publisher","first-page":"894","DOI":"10.1111\/jfb.15021","volume":"100","author":"J \u017d\u00e1k","year":"2022","unstructured":"\u017d\u00e1k J, Roy K, Dykov\u00e1 I, Mr\u00e1z J, Reichard M (2022) Starter feed for carnivorous species as a practical replacement of bloodworms for a vertebrate model organism in ageing, the turquoise killifish Nothobranchius furzeri. J Fish Biol 100:894\u2013908. https:\/\/doi.org\/10.1111\/jfb.15021","journal-title":"J Fish Biol"}],"container-title":["Cell and Tissue Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-024-03923-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00441-024-03923-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-024-03923-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T09:03:12Z","timestamp":1733216592000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00441-024-03923-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,21]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["3923"],"URL":"https:\/\/doi.org\/10.1007\/s00441-024-03923-5","relation":{},"ISSN":["0302-766X","1432-0878"],"issn-type":[{"value":"0302-766X","type":"print"},{"value":"1432-0878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,21]]},"assertion":[{"value":"1 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 October 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 October 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The Animal Welfare Office of the University of Naples Federico II reviewed the study, authorized by the Italian Ministry of Health in accordance with the Italian legislative Decree (n\u200026\/2014) (N\u00b0 291\/2022-PR).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}