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Among these genetic factors, the bovine Y-linked gene family, PRAMEY, has emerged as a candidate due to its involvement in germ cell formation, fertilization, and embryonic development. This study investigates PRAMEY\u2019s role in sperm-egg binding, acrosome integrity, and epigenetic modifications during fertilization and early embryogenesis. Using IVF with bovine spermatozoa treated with either PRAMEY antibody (ab) or rabbit IgG control, we assessed sperm-egg binding and acrosome integrity at 2, 4, and 6\u00a0h post-fertilization (hpf). PRAMEY ab treatment doubled sperm binding per oocyte across all time points, with a significant increase at 6 hpf (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.05), although no differences in acrosome integrity were observed (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). To explore PRAMEY\u2019s role in epigenetic regulation, we analyzed DNA (5-methylcytosine (5-mC)) and histone (H3K9me3 and H3K27me3) methylation in zygotes and embryos using\u00a0immunofluorescent staining  techniques. Zygotes derived from PRAMEY ab-treated spermatozoa showed significantly reduced DNA methylation in paternal pronuclei at 10 hpf and maternal pronuclei at 25 hpf (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.01). Histone methylation analysis revealed no significant differences in H3K9me3 methylation between groups, but H3K27me3 methylation was significantly lower in embryos produced using PRAMEY ab-treated spermatozoa at the 8-cell and morula stages (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.05). In summary, PRAMEY inhibition enhances sperm-egg binding and influences DNA and histone methylation dynamics in bovine embryos, underscoring its potential role in fertilization and early embryonic epigenetic regulation.<\/jats:p>","DOI":"10.1007\/s00441-025-03975-1","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:39:08Z","timestamp":1747193948000},"page":"83-94","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["PRAMEY enhances sperm-egg binding and modulates epigenetic dynamics in bovine embryogenesis"],"prefix":"10.1007","volume":"401","author":[{"given":"Chandlar","family":"Kern","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan-Sheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,5,14]]},"reference":[{"key":"3975_CR1","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1038\/nature18595","volume":"534","author":"H Aydin","year":"2016","unstructured":"Aydin H, Sultana A, Li S, Thavalingam A, Lee JE (2016) Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. 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