{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T21:19:09Z","timestamp":1793049549284,"version":"4.5.0"},"reference-count":74,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:00:00Z","timestamp":1756425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:00:00Z","timestamp":1756425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"The Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["22J12056"],"award-info":[{"award-number":["22J12056"]}]},{"name":"The Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["22K08363"],"award-info":[{"award-number":["22K08363"]}]},{"name":"The Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["17K08521"],"award-info":[{"award-number":["17K08521"]}]},{"name":"Foundation of Strategic Research Projects in Private Universities from the Ministry of Education, Culture, Sports, Science, and Technology of Japan","award":["S1311011 to Juntendo University"],"award-info":[{"award-number":["S1311011 to Juntendo University"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Tissue Res"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1007\/s00441-025-04002-z","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T02:32:39Z","timestamp":1756434759000},"page":"51-63","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Primary cilium disappearance in podocytes during vertebrate phylogeny revealed by array tomography"],"prefix":"10.1007","volume":"402","author":[{"given":"Takayuki","family":"Miyaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyuan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Sugiura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisako","family":"Kaneda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Alejandro Oliva","family":"Trejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuto","family":"Kawasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Omotehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takako","family":"Negishi-Koga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneaki","family":"Ishijima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soichiro","family":"Kakuta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichiro","family":"Ichimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,8,29]]},"reference":[{"key":"4002_CR1","first-page":"510","volume":"32","author":"PM Andrews","year":"1975","unstructured":"Andrews PM (1975) Scanning electron microscopy of human and rhesus monkey kidneys. Lab Invest 32:510\u2013518","journal-title":"Lab Invest"},{"key":"4002_CR2","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1093\/mollus\/59.2.223","volume":"59","author":"EB Andrews","year":"1993","unstructured":"Andrews EB, Jennings KH (1993) The anatomical and ultrastructural basis of primary urine formation in bivalve mollusks. J Molluscan Stud 59:223\u2013257","journal-title":"J Molluscan Stud"},{"key":"4002_CR3","doi-asserted-by":"publisher","first-page":"984452","DOI":"10.3389\/fendo.2022.984452","volume":"13","author":"Y Bai","year":"2022","unstructured":"Bai Y, Li P, Liu J, Zhang L, Cui S, Wei C, Fu B, Sun X, Cai G, Hong Q et al (2022) Renal primary cilia lengthen in the progression of diabetic kidney disease. Front Endocrinol 13:984452","journal-title":"Front Endocrinol"},{"key":"4002_CR4","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1046\/j.1523-1755.2000.00149.x","volume":"58","author":"L Barisoni","year":"2000","unstructured":"Barisoni L, Mokrzycki M, Sablay L, Nagata M, Yamase H, Mundel P (2000) Podocyte cell cycle regulation and proliferation in collapsing glomerulopathies. Kidney Int 58:137\u2013143","journal-title":"Kidney Int"},{"key":"4002_CR5","doi-asserted-by":"publisher","first-page":"201","DOI":"10.5858\/133.2.201","volume":"133","author":"L Barisoni","year":"2009","unstructured":"Barisoni L, Schnaper HW, Kopp JB (2009) Advances in the biology and genetics of the podocytopathies: implications for diagnosis and therapy. Arch Pathol Lab Med 133:201\u2013216","journal-title":"Arch Pathol Lab Med"},{"key":"4002_CR6","first-page":"F24","volume":"248","author":"D Beasley","year":"1985","unstructured":"Beasley D, Malvin RL (1985) Atrial extracts increase glomerular filtration rate in vivo. Am J Physiol 248:F24\u201330","journal-title":"Am J Physiol"},{"key":"4002_CR7","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1007\/BF00311947","volume":"106","author":"FB Beijnink","year":"1986","unstructured":"Beijnink FB, Voogt PA (1986) The aboral haemal system of the sea star, Asterias rubens (Echinodermata, Asteroidea): an ultrastructural and histochemical study. Zoomorphology 106:49\u201360","journal-title":"Zoomorphology"},{"key":"4002_CR8","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.cub.2009.11.072","volume":"20","author":"TY Besschetnova","year":"2010","unstructured":"Besschetnova TY, Kolpakova-Hart E, Guan Y, Zhou J, Olsen BR, Shah JV (2010) Identification of signaling pathways regulating primary cilium length and flow-mediated adaptation. Curr Biol 20:182\u2013187","journal-title":"Curr Biol"},{"key":"4002_CR9","doi-asserted-by":"crossref","unstructured":"Braun EJ, Dantzler WH (1997) Vertebrate renal system. In: Dantzler (ed) Handbook of physiology, section 13. Comparative physiology, 1st edn. Oxford University Press, Oxford","DOI":"10.1002\/cphy.cp130108"},{"key":"4002_CR10","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1242\/bio.20121016","volume":"1","author":"E Bubenshchikova","year":"2012","unstructured":"Bubenshchikova E, Ichimura K, Fukuyo Y, Powell R, Hsu C, Morrical SO, Sedor JR, Sakai T, Obara T (2012) Wtip and Vangl2 are required for mitotic spindle orientation and cloaca morphogenesis. Biol Open 1:588\u2013596","journal-title":"Biol Open"},{"key":"4002_CR11","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1007\/s40620-023-01730-9","volume":"37","author":"AG Carrasco","year":"2024","unstructured":"Carrasco AG, Izquierdo-Lahuerta A, de Pablos IG, Vila-Bedmar R, Martin-Taboada M, Porrini E, Morales E, Medina-Gomez G (2024) Detection of binucleated nephrin-marked podocytes by flow cytometry in the urine of patients with obesity. J Nephrol 37:245\u2013248","journal-title":"J Nephrol"},{"key":"4002_CR12","doi-asserted-by":"publisher","first-page":"2825","DOI":"10.1038\/sj.onc.1207528","volume":"23","author":"M Castedo","year":"2004","unstructured":"Castedo M, Perfettini JL, Roumier T, Andreau K, Medema R, Kroemer G (2004) Cell death by mitotic catastrophe: a molecular definition. Oncogene 23:2825\u20132837","journal-title":"Oncogene"},{"key":"4002_CR13","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1111\/nep.12022","volume":"18","author":"JA Deane","year":"2013","unstructured":"Deane JA, Verghese E, Martelotto LG, Cain JE, Galtseva A, Rosenblum ND, Watkins DN, Ricardo SD (2013) Visualizing renal primary cilia. Nephrology (Carlton) 18:161\u2013168","journal-title":"Nephrology (Carlton)"},{"key":"4002_CR14","doi-asserted-by":"publisher","first-page":"2349","DOI":"10.1056\/NEJMoa1614329","volume":"376","author":"A Denic","year":"2017","unstructured":"Denic A, Mathew J, Lerman LO, Lieske JC, Larson JJ, Alexander MP, Poggio E, Glassock RJ, Rule AD (2017) Single-nephron glomerular filtration rate in healthy adults. N Engl J Med 376:2349\u20132357","journal-title":"N Engl J Med"},{"key":"4002_CR15","doi-asserted-by":"publisher","first-page":"4033","DOI":"10.1242\/dev.036350","volume":"136","author":"NA Duldulao","year":"2009","unstructured":"Duldulao NA, Lee S, Sun Z (2009) Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b\/Scorpion. Development 136:4033\u20134042","journal-title":"Development"},{"key":"4002_CR16","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1161\/CIRCINTERVENTIONS.112.972596","volume":"5","author":"A Eirin","year":"2012","unstructured":"Eirin A, Ebrahimi B, Zhang X, Zhu XY, Tang H, Crane JA, Lerman A, Textor SC, Lerman LO (2012) Changes in glomerular filtration rate after renal revascularization correlate with microvascular hemodynamics and inflammation in Swine renal artery stenosis. Circ Cardiovasc Interv 5:720\u2013728","journal-title":"Circ Cardiovasc Interv"},{"key":"4002_CR17","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1146\/annurev-physiol-031522-084334","volume":"85","author":"O Esarte Palomero","year":"2023","unstructured":"Esarte Palomero O, Larmore M, DeCaen PG (2023) Polycystin channel complexes. Annu Rev Physiol 85:425\u2013448","journal-title":"Annu Rev Physiol"},{"key":"4002_CR18","doi-asserted-by":"publisher","unstructured":"Fitzsimons L (2024) An emerging role for primary cilia of the renal glomerulus: implications and considerations for pathogenesis of glomerular diseases. In: Heinbockel T (ed) Cell communication and signaling in health and disease, 1st edn. IntechOpen, London.\u00a0https:\/\/doi.org\/10.5772\/intechopen.114508","DOI":"10.5772\/intechopen.114508"},{"key":"4002_CR19","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1007\/s12565-015-0317-7","volume":"92","author":"K Ichimura","year":"2017","unstructured":"Ichimura K, Sakai T (2017) Evolutionary morphology of podocytes and primary urine-producing apparatus. Anat Sci Int 92:161\u2013172","journal-title":"Anat Sci Int"},{"key":"4002_CR20","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1007\/s00441-007-0440-4","volume":"329","author":"K Ichimura","year":"2007","unstructured":"Ichimura K, Kurihara H, Sakai T (2007) Actin filament organization of foot processes in vertebrate glomerular podocytes. Cell Tissue Res 329:541\u2013557","journal-title":"Cell Tissue Res"},{"key":"4002_CR21","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1681\/ASN.2007101138","volume":"19","author":"K Ichimura","year":"2008","unstructured":"Ichimura K, Stan RV, Kurihara H, Sakai T (2008) Glomerular endothelial cells form diaphragms during development and pathologic conditions. J Am Soc Nephrol 19:1463\u20131471","journal-title":"J Am Soc Nephrol"},{"key":"4002_CR22","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1007\/s00441-010-0983-7","volume":"341","author":"K Ichimura","year":"2010","unstructured":"Ichimura K, Kurihara H, Sakai T (2010) Primary cilia disappear in rat podocytes during glomerular development. Cell Tissue Res 341:197\u2013209","journal-title":"Cell Tissue Res"},{"key":"4002_CR23","doi-asserted-by":"publisher","first-page":"8993","DOI":"10.1038\/srep08993","volume":"5","author":"K Ichimura","year":"2015","unstructured":"Ichimura K, Miyazaki N, Sadayama S, Murata K, Koike M, Nakamura K, Ohta K, Sakai T (2015) Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy. Sci Rep 5:8993","journal-title":"Sci Rep"},{"key":"4002_CR24","first-page":"132","volume":"130","author":"K Ichimura","year":"2017","unstructured":"Ichimura K, Kakuta S, Kawasaki Y, Miyaki T, Nonami T, Miyazaki N, Nakao T, Enomoto S, Arai S, Koike M et al (2017) Morphological process of podocyte development revealed by block-face scanning electron microscopy. J Cell Sci 130:132\u2013142","journal-title":"J Cell Sci"},{"key":"4002_CR25","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1681\/ASN.2018020139","volume":"30","author":"K Ichimura","year":"2019","unstructured":"Ichimura K, Miyaki T, Kawasaki Y, Kinoshita M, Kakuta S, Sakai T (2019) Morphological processes of foot process effacement in puromycin aminonucleoside nephrosis revealed by FIB\/SEM tomography. J Am Soc Nephrol 30:96\u2013108","journal-title":"J Am Soc Nephrol"},{"key":"4002_CR26","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1016\/S0022-5320(66)80001-1","volume":"16","author":"U Karlsson","year":"1966","unstructured":"Karlsson U (1966) Three-dimensional studies of neurons in the lateral geniculate nucleus of the rat. I. Organelle organization in the perikaryon and its proximal branches. J Ultrastruct Res 16:429\u2013481","journal-title":"J Ultrastruct Res"},{"key":"4002_CR27","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.2460\/ajvr.73.9.1344","volume":"73","author":"R Katayama","year":"2012","unstructured":"Katayama R, Saito J, Katayama M, Yamagishi N, Yamashita T, Kato M, Furuhama K (2012) Simplified procedure for the estimation of glomerular filtration rate following intravenous administration of iodixanol in cats. Am J Vet Res 73:1344\u20131349","journal-title":"Am J Vet Res"},{"key":"4002_CR28","doi-asserted-by":"publisher","first-page":"653472","DOI":"10.3389\/fcell.2021.653472","volume":"9","author":"Y Kawasaki","year":"2021","unstructured":"Kawasaki Y, Hosoyamada Y, Miyaki T, Yamaguchi J, Kakuta S, Sakai T, Ichimura K (2021) Three-dimensional architecture of glomerular endothelial cells revealed by FIB-SEM tomography. Front Cell Dev Biol 9:653472","journal-title":"Front Cell Dev Biol"},{"key":"4002_CR29","doi-asserted-by":"publisher","first-page":"2","DOI":"10.14789\/jmj.2021.67.JMJ21-R01","volume":"67","author":"Y Kawasaki","year":"2021","unstructured":"Kawasaki Y, Miyaki T, Yamaguchi J, Kakuta S, Hosoyamada Y, Sakai T, Ichimura K (2021) Application of conventional FE-SEM to podocyte structural analysis: protocol and usage examples. Juntendo Med J 67:2\u20139","journal-title":"Juntendo Med J"},{"key":"4002_CR30","first-page":"S171","volume":"63","author":"I Kihara","year":"1997","unstructured":"Kihara I, Tsuchida S, Yaoita E, Yamamoto T, Hara M, Yanagihara T, Takada T (1997) Podocyte detachment and epithelial cell reaction in focal segmental glomerulosclerosis with cellular variants. Kidney Int Suppl 63:S171\u2013S176","journal-title":"Kidney Int Suppl"},{"key":"4002_CR31","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1111\/j.1365-2559.1999.00670.x","volume":"34","author":"I Kihara","year":"1999","unstructured":"Kihara I, Yaoita E, Kawasaki K, Yamamoto T, Hara M, Yanagihara T (1999) Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy. Histopathology 34:537\u2013547","journal-title":"Histopathology"},{"key":"4002_CR32","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1038\/ki.1978.86","volume":"14","author":"JI Kreisberg","year":"1978","unstructured":"Kreisberg JI, Hoover RL, Karnovsky MJ (1978) Isolation and characterization of rat glomerular epithelial cells in vitro. Kidney Int 14:21\u201330","journal-title":"Kidney Int"},{"key":"4002_CR33","doi-asserted-by":"publisher","first-page":"1352377","DOI":"10.3389\/fphar.2024.1352377","volume":"15","author":"S Li","year":"2024","unstructured":"Li S, Fan R, Wang Y, He K, Xu J, Li H (2024) Application of calcium overload-based ion interference therapy in tumor treatment: strategies, outcomes, and prospects. Front Pharmacol 15:1352377","journal-title":"Front Pharmacol"},{"key":"4002_CR34","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/s00435-003-0078-z","volume":"122","author":"G Mayer","year":"2003","unstructured":"Mayer G, Bartolomaeus T (2003) Ultrastructure of the stomochord and the heart\u2013glomerulus complex in Rhabdopleura compacta (Pterobranchia): phylogenetic implications. Zoomorphology 122:125\u2013133","journal-title":"Zoomorphology"},{"key":"4002_CR35","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.ejphar.2012.10.032","volume":"698","author":"A Mehta","year":"2013","unstructured":"Mehta A, Prabhakar M, Kumar P, Deshmukh R, Sharma PL (2013) Excitotoxicity: bridge to various triggers in neurodegenerative disorders. Eur J Pharmacol 698:6\u201318","journal-title":"Eur J Pharmacol"},{"key":"4002_CR36","doi-asserted-by":"publisher","first-page":"1262","DOI":"10.2460\/ajvr.1978.39.08.1262","volume":"39","author":"HD Mercer","year":"1978","unstructured":"Mercer HD, Willett LB, Schandbacher FL, Moorhead PD, Powers TE (1978) Use of the double-isotope, single-injection method for estimating renal function in normal and polybrominated biphenyl-exposed dairy cows. Am J Vet Res 39:1262\u20131268","journal-title":"Am J Vet Res"},{"key":"4002_CR37","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1258\/la.2012.011065","volume":"46","author":"Y Michigoshi","year":"2012","unstructured":"Michigoshi Y, Katayama R, Yamagishi N, Kato M, Saito J, Satoh H, Furuhama K (2012) Estimation of glomerular filtration rate in rabbits by a single-sample method using iodixanol. Lab Anim 46:341\u2013344","journal-title":"Lab Anim"},{"key":"4002_CR38","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1038\/s41576-023-00587-9","volume":"24","author":"P Mill","year":"2023","unstructured":"Mill P, Christensen ST, Pedersen LB (2023) Primary cilia as dynamic and diverse signalling hubs in development and disease. Nat Rev Genet 24:421\u2013441","journal-title":"Nat Rev Genet"},{"key":"4002_CR39","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1007\/s00441-019-03118-3","volume":"379","author":"T Miyaki","year":"2020","unstructured":"Miyaki T, Kawasaki Y, Hosoyamada Y, Amari T, Kinoshita M, Matsuda H, Kakuta S, Sakai T, Ichimura K (2020) Three-dimensional imaging of podocyte ultrastructure using FE-SEM and FIB-SEM tomography. Cell Tissue Res 379:245\u2013254","journal-title":"Cell Tissue Res"},{"key":"4002_CR40","doi-asserted-by":"publisher","first-page":"180","DOI":"10.14789\/jmj.2020.66.JMJ19-R19","volume":"66","author":"T Miyaki","year":"2020","unstructured":"Miyaki T, Kawasaki Y, Hosoyamada Y, Amari T, Kinoshita M, Matsuda H, Kakuta S, Sakai T, Ichimura K (2020) Volume scanning electron microscopy for 3D analysis of biological ultrastructure. Juntendo Med J 66:180\u2013119","journal-title":"Juntendo Med J"},{"key":"4002_CR41","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1007\/s00441-020-03313-7","volume":"382","author":"T Miyaki","year":"2020","unstructured":"Miyaki T, Kawasaki Y, Matsumoto A, Kakuta S, Sakai T, Ichimura K (2020) Nephrocytes are part of the spectrum of filtration epithelial diversity. Cell Tissue Res 382:609\u2013625","journal-title":"Cell Tissue Res"},{"key":"4002_CR42","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.262154","author":"T Miyaki","year":"2024","unstructured":"Miyaki T, Homma N, Kawasaki Y, Kishi M, Yamaguchi J, Kakuta S, Shindo T, Sugiura M, Oliva Trejo JA, Kaneda H et al (2024) Ultrastructural analysis of whole glomeruli using array tomography. J Cell Sci. https:\/\/doi.org\/10.1242\/jcs.262154","journal-title":"J Cell Sci"},{"key":"4002_CR43","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/BF00220622","volume":"187","author":"M Miyoshi","year":"1978","unstructured":"Miyoshi M (1978) Scanning electron microscopy of the renal corpuscle of the mesonephros in the lamprey, Entosphenus japonicus Martens. Cell Tissue Res 187:105\u2013113","journal-title":"Cell Tissue Res"},{"key":"4002_CR44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BF00224314","volume":"148","author":"PC Moller","year":"1974","unstructured":"Moller PC, Ellis RA (1974) Fine structure of the excretory system of Amphioxus (Branchiostoma floridae) and its response to osmotic stress. Cell Tissue Res 148:1\u20139","journal-title":"Cell Tissue Res"},{"key":"4002_CR45","doi-asserted-by":"publisher","first-page":"800074","DOI":"10.3389\/fimmu.2021.800074","volume":"12","author":"AK Muehlig","year":"2021","unstructured":"Muehlig AK, Gies S, Huber TB, Braun F (2021) Collapsing focal segmental glomerulosclerosis in viral infections. Front Immunol 12:800074","journal-title":"Front Immunol"},{"key":"4002_CR46","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1093\/ndt\/gfx201","volume":"33","author":"J Muhldorfer","year":"2018","unstructured":"Muhldorfer J, Pfister E, Buttner-Herold M, Klewer M, Amann K, Daniel C (2018) Bi-nucleation of podocytes is uniformly accompanied by foot processes widening in renal disease. Nephrol Dial Transplant 33:796\u2013803","journal-title":"Nephrol Dial Transplant"},{"key":"4002_CR47","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1002\/path.4193","volume":"230","author":"SR Mulay","year":"2013","unstructured":"Mulay SR, Thomasova D, Ryu M, Kulkarni OP, Migliorini A, Bruns H, Grobmayr R, Lazzeri E, Lasagni L, Liapis H et al (2013) Podocyte loss involves MDM2-driven mitotic catastrophe. J Pathol 230:322\u2013335","journal-title":"J Pathol"},{"key":"4002_CR48","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1016\/j.kint.2016.01.012","volume":"89","author":"M Nagata","year":"2016","unstructured":"Nagata M (2016) Podocyte injury and its consequences. Kidney Int 89:1221\u20131230","journal-title":"Kidney Int"},{"key":"4002_CR49","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1007\/BF00195765","volume":"187","author":"M Nagata","year":"1993","unstructured":"Nagata M, Yamaguchi Y, Ito K (1993) Loss of mitotic activity and the expression of vimentin in glomerular epithelial cells of developing human kidneys. Anat Embryol (Berl) 187:275\u2013279","journal-title":"Anat Embryol (Berl)"},{"key":"4002_CR50","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1159\/000188546","volume":"70","author":"M Nagata","year":"1995","unstructured":"Nagata M, Yamaguchi Y, Komatsu Y, Ito K (1995) Mitosis and the presence of binucleate cells among glomerular podocytes in diseased human kidneys. Nephron 70:68\u201371","journal-title":"Nephron"},{"key":"4002_CR51","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1681\/ASN.2004100822","volume":"16","author":"CR Neal","year":"2005","unstructured":"Neal CR, Crook H, Bell E, Harper SJ, Bates DO (2005) Three-dimensional reconstruction of glomeruli by electron microscopy reveals a distinct restrictive urinary subpodocyte space. J Am Soc Nephrol 16:1223\u20131235","journal-title":"J Am Soc Nephrol"},{"key":"4002_CR52","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1002\/ar.a.10068","volume":"272","author":"JL Ojeda","year":"2003","unstructured":"Ojeda JL, Icardo JM, Domezain A (2003) Renal corpuscle of the sturgeon kidney: an ultrastructural, chemical dissection, and lectin-binding study. Anat Rec A Discov Mol Cell Evol Biol 272:563\u2013573","journal-title":"Anat Rec A Discov Mol Cell Evol Biol"},{"key":"4002_CR53","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1152\/physrev.00020.2002","volume":"83","author":"H Pavenstadt","year":"2003","unstructured":"Pavenstadt H, Kriz W, Kretzler M (2003) Cell biology of the glomerular podocyte. Physiol Rev 83:253\u2013307","journal-title":"Physiol Rev"},{"key":"4002_CR54","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1083\/jcb.17.1.208","volume":"17","author":"ES Reynolds","year":"1963","unstructured":"Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208\u2013212","journal-title":"J Cell Biol"},{"key":"4002_CR55","first-page":"3697","volume":"57","author":"A Ruffo","year":"1966","unstructured":"Ruffo A, Rossotto P, Motta G, Martines G, Musacci GF (1966) Ultrastructural findings in deep renal hypothermia. Minerva Med 57:3697\u20133721","journal-title":"Minerva Med"},{"key":"4002_CR56","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1111\/j.1469-185X.1988.tb00631.x","volume":"63","author":"E Ruppert","year":"1988","unstructured":"Ruppert E, Smith P (1988) The functional organization of filtration nephridia. Biol Rev 63:231\u2013258","journal-title":"Biol Rev"},{"key":"4002_CR57","unstructured":"Ruppert E, Fox R, Barnes R (2003) Invertebrate zoology, a functional evolutionary approach, 7th edn. Brooks\/Cole Thompson\u00a0Learning, Belmont"},{"key":"4002_CR58","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1038\/s41467-023-44442-1","volume":"15","author":"Y Sakai","year":"2024","unstructured":"Sakai Y, Takahashi S, Koyama-Honda I, Saito C, Mizushima N (2024) Experimental determination and mathematical modeling of standard shapes of forming autophagosomes. Nat Commun 15:91","journal-title":"Nat Commun"},{"key":"4002_CR59","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12135","author":"Y Sasaki","year":"2014","unstructured":"Sasaki Y, Iwama R, Sato T, Heishima K, Shimamura S, Ichijo T, Satoh H, Furuhama K (2014) Estimation of glomerular filtration rate in conscious mice using a simplified equation. Physiol Rep. https:\/\/doi.org\/10.14814\/phy2.12135","journal-title":"Physiol Rep"},{"key":"4002_CR60","doi-asserted-by":"publisher","first-page":"828","DOI":"10.2460\/ajvr.76.9.828","volume":"76","author":"Y Sasaki","year":"2015","unstructured":"Sasaki Y, Iwama R, Shimamura S, Katayama M, Uzuka Y, Yabe K, Takasuna K, Satoh H, Furuhama K (2015) Estimation of glomerular filtration rate in dogs by a single-blood sample method involving iodixanol. Am J Vet Res 76:828\u2013835","journal-title":"Am J Vet Res"},{"key":"4002_CR61","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1007\/s11259-011-9485-7","volume":"35","author":"H Satoh","year":"2011","unstructured":"Satoh H, Abe S, Kato M, Saito J, Furuhama K (2011) Optimum conditions for serum clearance of iodixanol, applicable to the estimation of glomerular filtration rate in horses. Vet Res Commun 35:463\u2013468","journal-title":"Vet Res Commun"},{"key":"4002_CR62","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1002\/jat.3178","volume":"36","author":"H Satoh","year":"2016","unstructured":"Satoh H, Nomiya N, Imai D, Sato S, Sakurai K, Takasuna K, Furuhama K (2016) A method for estimating the glomerular filtration rate in conscious monkeys. J Appl Toxicol 36:266\u2013270","journal-title":"J Appl Toxicol"},{"key":"4002_CR63","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1038\/s41588-017-0020-9","volume":"50","author":"JE Siljee","year":"2018","unstructured":"Siljee JE, Wang Y, Bernard AA, Ersoy BA, Zhang S, Marley A, Von Zastrow M, Reiter JF, Vaisse C (2018) Subcellular localization of MC4R with ADCY3 at neuronal primary cilia underlies a common pathway for genetic predisposition to obesity. Nat Genet 50:180\u2013185","journal-title":"Nat Genet"},{"key":"4002_CR64","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1097\/MNH.0000000000000873","volume":"32","author":"KD Smith","year":"2023","unstructured":"Smith KD, Akilesh S (2023) Collapsing glomerulopathy: unraveling varied pathogeneses. Curr Opin Nephrol Hypertens 32:213\u2013222","journal-title":"Curr Opin Nephrol Hypertens"},{"key":"4002_CR65","doi-asserted-by":"publisher","first-page":"158","DOI":"10.22203\/eCM.v033a12","volume":"33","author":"M Spasic","year":"2017","unstructured":"Spasic M, Jacobs CR (2017) Lengthening primary cilia enhances cellular mechanosensitivity. Eur Cells Mater 33:158\u2013168","journal-title":"Eur Cells Mater"},{"key":"4002_CR66","doi-asserted-by":"publisher","first-page":"100857","DOI":"10.1016\/j.xkme.2024.100857","volume":"6","author":"Y Taniguchi","year":"2024","unstructured":"Taniguchi Y, Miura K, Shira Y, Fujimaru T, Sohara E, Yamaguchi Y, Hattori M (2024) Primary cilia elongation in early-onset polycystic kidney disease with 2 hypomorphic PKD1 alleles: a case report. Kidney Med 6:100857","journal-title":"Kidney Med"},{"key":"4002_CR67","doi-asserted-by":"publisher","DOI":"10.1172\/JCI171237","author":"X Tian","year":"2023","unstructured":"Tian X, Pedigo CE, Li K, Ma X, Bunda P, Pell J, Lek A, Gu J, Zhang Y, Medina Rangel PX et al (2023) Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases. J Clin Invest. https:\/\/doi.org\/10.1172\/JCI171237","journal-title":"J Clin Invest"},{"key":"4002_CR68","doi-asserted-by":"publisher","first-page":"2147","DOI":"10.1681\/ASN.2008101105","volume":"20","author":"E Verghese","year":"2009","unstructured":"Verghese E, Ricardo SD, Weidenfeld R, Zhuang J, Hill PA, Langham RG, Deane JA (2009) Renal primary cilia lengthen after acute tubular necrosis. J Am Soc Nephrol 20:2147\u20132153","journal-title":"J Am Soc Nephrol"},{"key":"4002_CR69","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1016\/j.acthis.2018.08.015","volume":"120","author":"K Vukojevic","year":"2018","unstructured":"Vukojevic K, Raguz F, Saraga M, Filipovic N, Bocina I, Kero D, Glavina Durdov M, Martinovic V, Saraga-Babic M (2018) Glomeruli from patients with nephrin mutations show increased number of ciliated and poorly differentiated podocytes. Acta Histochem 120:748\u2013756","journal-title":"Acta Histochem"},{"key":"4002_CR70","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1681\/ASN.V361279","volume":"3","author":"T Weinstein","year":"1992","unstructured":"Weinstein T, Cameron R, Katz A, Silverman M (1992) Rat glomerular epithelial cells in culture express characteristics of parietal, not visceral, epithelium. J Am Soc Nephrol 3:1279\u20131287","journal-title":"J Am Soc Nephrol"},{"key":"4002_CR71","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/j.1469-185X.1974.tb01572.x","volume":"49","author":"RA Wilson","year":"1974","unstructured":"Wilson RA, Webster LA (1974) Protonephridia. Biol Rev Camb Philos Soc 49:127\u2013160","journal-title":"Biol Rev Camb Philos Soc"},{"key":"4002_CR72","first-page":"136","volume":"67","author":"E Yaoita","year":"1995","unstructured":"Yaoita E, Yamamoto T, Takashima N, Kawasaki K, Kawachi H, Shimizu F, Kihara I (1995) Visceral epithelial cells in rat glomerular cell culture. Eur J Cell Biol 67:136\u2013144","journal-title":"Eur J Cell Biol"},{"key":"4002_CR73","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1002\/path.1711360207","volume":"136","author":"N Yoshikawa","year":"1982","unstructured":"Yoshikawa N, Cameron AH, White RH (1982) Ultrastructure of the non-sclerotic glomeruli in childhood nephrotic syndrome. J Pathol 136:133\u2013147","journal-title":"J Pathol"},{"key":"4002_CR74","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1007\/BF00206268","volume":"228","author":"A Zuasti","year":"1983","unstructured":"Zuasti A, Agulleiro B, Hernandez F (1983) Ultrastructure of the kidney of the marine teleost Sparus auratus: the renal corpuscle and the tubular nephron. Cell Tissue Res 228:99\u2013106","journal-title":"Cell Tissue Res"}],"container-title":["Cell and Tissue Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-025-04002-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00441-025-04002-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00441-025-04002-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T20:04:52Z","timestamp":1759262692000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00441-025-04002-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,29]]},"references-count":74,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["4002"],"URL":"https:\/\/doi.org\/10.1007\/s00441-025-04002-z","relation":{},"ISSN":["0302-766X","1432-0878"],"issn-type":[{"value":"0302-766X","type":"print"},{"value":"1432-0878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,29]]},"assertion":[{"value":"6 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 August 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All procedures performed on laboratory animals were approved by the Institutional Animal Care and Use Committee of Juntendo University School of Medicine (Approval No. 2020104). All animal experiments were performed in accordance with the Guidelines for Animal Experimentation of Juntendo University School of Medicine.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}