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Notably, ICCs are excitable cells that generate characteristic oscillations in pacemaker activity mediated by the cytoplasmic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration. Since the primary source of cytoplasmic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    is the endoplasmic reticulum (ER) or sarcoplasmic reticulum (SR), its distribution and localisation in the cytoplasm may indicate characteristic aspects that help in understanding its physiological functions. Furthermore, caveolae, which are invaginations of the plasma membrane (PM) observed in both ICCs and SMCs, are key sites for Ca\n                    <jats:sup>2+<\/jats:sup>\n                    sparks, and their functions have been well studied in muscle cells. However, the mechanism by which caveolae and ER interact to regulate Ca\n                    <jats:sup>2+<\/jats:sup>\n                    in ICCs remains unclear compared with that in SMCs. In this study, microdomains comprising the PM, caveolae, and ER\/SR were analysed using focused ion beam\/scanning electron microscopy (FIB\/SEM) to provide new insights into ICC functional analysis. Additionally, as mitochondria could regulate local Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration, mitochondria-associated membrane (MAM) contacts with the ER were also analysed in ICCs. Novel ER ultrastructures with distinct characteristics and distributions were identified in each cell type by FIB\/SEM. Furthermore, reconstructed three-dimensional (3D) images enabled measurement of the distance between the PM and ER, as well as the MAM areas, thus contributing to a better understanding of ICC physiological features. These new morphological insights may help resolve controversial interpretations in physiological and pharmacological studies of ICCs.\n                  <\/jats:p>","DOI":"10.1007\/s00441-025-04016-7","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T07:19:24Z","timestamp":1761635964000},"page":"333-344","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["ER morphological analysis associated with interstitial cells of Cajal and smooth muscle cells in the murine stomach"],"prefix":"10.1007","volume":"402","author":[{"given":"Hiromi","family":"Tamada","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Iino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"4016_CR1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.202213217","volume":"155","author":"EJ Akin","year":"2023","unstructured":"Akin EJ, Aoun J, Jimenez C, Mayne K, Baeck J, Young MD, Sullivan B, Sanders KM, Ward SM, Bulley S, Jaggar JH, Earley S, Greenwood IA, Leblanc N (2023) ANO1, CaV1.2, and IP3R form a localized unit of EC-coupling in mouse pulmonary arterial smooth muscle. 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It should read as below:","order":10,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"In this study, a spherical region 400 nm in radius from the centred caveolae was used for geometrical analysis.","order":11,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The original article has been corrected.","order":12,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 November 2025","order":13,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Correction","order":14,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"A Correction to this paper has been published:","order":15,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"https:\/\/doi.org\/10.1007\/s00441-025-04026-5","URL":"https:\/\/doi.org\/10.1007\/s00441-025-04026-5","order":16,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were conducted in accordance with the Guidelines for Animal Experiments and the Regulations for Animal Research at the University of Fukui.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}