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The need for an alternative hemoprotein source has emerged due to the inherent risks of large-scale livestock farming and animal proteins. <jats:italic>Corynebacterium glutamicum<\/jats:italic>, regarded for Qualified Presumption of Safety or Generally Recognized as Safe, can biosynthesize hemoproteins. <jats:italic>C. glutamicum<\/jats:italic> single-cell protein (SCP) can be a valuable alternative hemoprotein for supplying heme iron without adversely affecting blood fat levels. We constructed the chemostat culture system to increase hemoprotein content in <jats:italic>C. glutamicum<\/jats:italic> SCP. Through adaptive evolution, hemoprotein levels could be naturally increased to address oxidative stress resulting from enhanced growth rate. In addition, we used several specific plasmids containing growth-accelerating genes and the <jats:italic>hemA<\/jats:italic> promoter to expedite the evolutionary process. Following chemostat culture for 15\u00a0days, the plasmid in selected descendants was cured. The evolved strains showed improved specific growth rates from 0.59\u00a0h<jats:sup>\u22121<\/jats:sup> to 0.62\u00a0h<jats:sup>\u22121<\/jats:sup>, 20% enhanced resistance to oxidative stress, and increased heme concentration from 12.95\u00a0\u00b5g\/g-DCW to 14.22\u201315.24\u00a0\u00b5g\/g-DCW. Notably, the putative peptidyl-tRNA hydrolase-based evolved strain manifested the most significant increase (30%) of hemoproteins. This is the first report presenting the potential of a growth-acceleration-targeted evolution (GATE) strategy for developing non-GMO industrial strains with increased bio-product productivity.<\/jats:p>","DOI":"10.1007\/s00449-024-02986-6","type":"journal-article","created":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T18:01:47Z","timestamp":1710784907000},"page":"549-556","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development strategy of non-GMO organism for increased hemoproteins in Corynebacterium glutamicum: a growth-acceleration-targeted evolution"],"prefix":"10.1007","volume":"47","author":[{"given":"Sehyeon","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seungki","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taeyeon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahyoung","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soyeon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pil","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,3,18]]},"reference":[{"issue":"5","key":"2986_CR1","first-page":"671","volume":"75","author":"S Killip","year":"2007","unstructured":"Killip S, Bennett JM, Chambers MD (2007) Iron deficiency anemia. 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The funders had no role in the design of the study and the interpretation of data.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Mice were purchased from Hana Biotech Inc. (Pyeongtaek, Korea) and reared for 4\u00a0weeks in an animal breeding facility (animal experimental facility registration #412, South-east Medi-Chem Institute Inc., Busan, South Korea). Animal experiments were performed according to the guidelines of the institutional animal care and use committee (protocol No. SEMI-22-001). All feasible measures were taken to reduce animal suffering.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}]}}