{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T06:06:41Z","timestamp":1784268401862,"version":"3.55.0"},"reference-count":61,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universidade Federal De S\u00e3o Carlos"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Bioprocess Biosyst Eng"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In situ cellulase production is a key strategy to enhance efficiency, sustainability, and robustness of second-generation bioethanol processes. However, limitations related to heat transfer, bioreactor operation, and downstream processing still hinder large-scale implementation of solid-state fermentation (SSF) for enzymes production. This study systematically evaluated the integrated production, extraction, recovery and stabilization of endoglucanase from the cultivation of\n                    <jats:italic>Myceliophthora thermophila<\/jats:italic>\n                    I-1D3b in a multilayer packed-bed bioreactor operated under batch and continuous modes, using sugarcane bagasse and wheat bran (7:3 w\/w) as substrate. Fermentations were carried at 45\u00a0\u00b0C for 96\u00a0h, and the enzymatic extracts were characterized as a function of pH and temperature, as well as subjected to concentration by ammonium sulfate and ethanol precipitation. Maximum enzymatic activity was achieved at 60\u00a0\u00b0C and pH 4.0, confirming a thermoacidophilic profile suitable for industrial applications. Ammonium sulfate precipitation outperformed ethanol, achieving 78.7% of recovering of total units of enzymatic activity from the raw extract and an activity of 156 U\u00b7g.d.s\u207b\u00b9 (enzyme units per gram of dry substrate), whereas ethanol resulted in significant activity losses under the evaluated conditions. Within the bioreactor, maximum temperatures reached 48.2\u00a0\u00b0C (batch) and 48.9\u00a0\u00b0C (continuous), indicating effective mitigation of overheating. Increasing percolation water flow rate significantly enhanced enzyme recovery, highlighting mass transfer as a key limiting factor in downstream performance. Similar average activities were obtained for batch (95.63\u2009\u00b1\u200928.37 U\u00b7g.d.s\u207b\u00b9) and continuous operation (96.36\u2009\u00b1\u20094.63 U\u00b7g.d.s\u207b\u00b9), while continuous mode reduced variability among modules, indicating improved spatial homogeneity. Overall, process integration combining thermal control, percolation-based extraction, and downstream processing enhances robustness and industrial applicability of SSF-based cellulase production.\n                  <\/jats:p>","DOI":"10.1007\/s00449-026-03371-1","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T08:26:23Z","timestamp":1782721583000},"page":"1939-1957","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated cellulase continuous production and downstream processing using a packed-bed bioreactor for solid-state fermentation by thermophilic fungus"],"prefix":"10.1007","volume":"49","author":[{"given":"Nilton S. C.","family":"Mafra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9274-1241","authenticated-orcid":false,"given":"Fernanda P.","family":"Casciatori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"3371_CR1","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1016\/j.psep.2024.01.055","volume":"183","author":"MS Wagh","year":"2024","unstructured":"Wagh MS, Nath SS, Chakraborty PC, Amrit A, Mishra R, Mishra B, Mohanta AK YK (2024) Valorisation of agro-industrial wastes: Circular bioeconomy and biorefinery process \u2013 A sustainable symphony. Process Saf Environ Prot 183:708\u2013725. https:\/\/doi.org\/10.1016\/j.psep.2024.01.055","journal-title":"Process Saf Environ Prot"},{"key":"3371_CR2","doi-asserted-by":"publisher","first-page":"161904","DOI":"10.1016\/j.scitotenv.2023.161904","volume":"870","author":"PK Sadh","year":"2023","unstructured":"Sadh PK, Chawla P, Kumar S, Das A, Kumar R, Bains A, Sridhar K, Duhan JS, Sharma M (2023) Recovery of agricultural waste biomass: A path for circular bioeconomy. Sci Total Environ 870:161904. https:\/\/doi.org\/10.1016\/j.scitotenv.2023.161904","journal-title":"Sci Total Environ"},{"key":"3371_CR3","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1021\/acscentsci.3c01372","volume":"10","author":"C Duan","year":"2024","unstructured":"Duan C, Wang R (2024) A unified description of salt effects on the liquid\u2013liquid phase separation of proteins. ACS Cent Sci 10:460\u2013468. https:\/\/doi.org\/10.1021\/acscentsci.3c01372","journal-title":"ACS Cent Sci"},{"key":"3371_CR4","doi-asserted-by":"publisher","first-page":"1630","DOI":"10.1021\/acssusresmgt.4c00062","volume":"1","author":"A S\u00e1nchez","year":"2024","unstructured":"S\u00e1nchez A (2024) A perspective of solid-state fermentation as emergent technology for organic waste management in the framework of circular bioeconomy. ACS Sustain Resour Manag 1:1630\u20131638. https:\/\/doi.org\/10.1021\/acssusresmgt.4c00062","journal-title":"ACS Sustain Resour Manag"},{"key":"3371_CR5","doi-asserted-by":"publisher","first-page":"114692","DOI":"10.1016\/j.rser.2024.114692","volume":"202","author":"K Ding","year":"2024","unstructured":"Ding K, Liu D, Chen X, Zhang H, Shi S, Guo X, Zhou L, Han L, Xiao W (2024) Scalable lignocellulosic biorefineries: Technoeconomic review for efficient fermentable sugars production. Renew Sustain Energy Rev 202:114692. https:\/\/doi.org\/10.1016\/j.rser.2024.114692","journal-title":"Renew Sustain Energy Rev"},{"key":"3371_CR6","doi-asserted-by":"publisher","unstructured":"G\u00f3mez-Ramos GA, Prado\u2010Barrag\u00e1n LA, Rivera BA, Huerta\u2010Ochoa S, Rivera VM, Couder\u2010Garc\u00eda M, Garc\u00eda\u2010Hern\u00e1ndez R, de los \u00c1, Castillo\u2010Araiza CO (2025) Bioreactor engineering for circular economy: bioactive compound production in solid\u2010state fermentation\u2014review. Chem Eng Technol 48:e202400289. https:\/\/doi.org\/10.1002\/ceat.202400289","DOI":"10.1002\/ceat.202400289"},{"key":"3371_CR7","doi-asserted-by":"publisher","first-page":"481","DOI":"10.3390\/fermentation10090481","volume":"10","author":"A Sala","year":"2024","unstructured":"Sala A, Artola A, Barrena R, S\u00e1nchez A (2024) Harnessing packed-bed bioreactors\u2019 potential in solid-state fermentation: The case of Beauveria bassiana conidia production. Fermentation 10:481. https:\/\/doi.org\/10.3390\/fermentation10090481","journal-title":"Fermentation"},{"key":"3371_CR8","doi-asserted-by":"publisher","first-page":"872","DOI":"10.3390\/pr11030872","volume":"11","author":"DA Mitchell","year":"2023","unstructured":"Mitchell DA, Ruiz HA, Krieger N (2023) A critical evaluation of recent studies on packed-bed bioreactors for solid-state fermentation. Processes 11:872. https:\/\/doi.org\/10.3390\/pr11030872","journal-title":"Processes"},{"key":"3371_CR9","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1007\/s12155-023-10675-7","volume":"17","author":"FP Casciatori","year":"2024","unstructured":"Casciatori FP, Mafra NSC, Rodrigues NA, Sales EH, Maia MMS, Dias ACP, Grajales LM (2024) Flow configuration and packing density of sugarcane bagasse and wheat bran affecting enzymes production by solid-state fermentation. Bioenergy Res 17:187\u2013197. https:\/\/doi.org\/10.1007\/s12155-023-10675-7","journal-title":"Bioenergy Res"},{"key":"3371_CR10","doi-asserted-by":"publisher","first-page":"137726","DOI":"10.1016\/j.cej.2022.137726","volume":"448","author":"NA Rodrigues","year":"2022","unstructured":"Rodrigues NA, Katayama ET, Casciatori FP (2022) Alternative strategies to perform solid-state cultivation in a multilayer packed-bed bioreactor: Continuous and cyclic operations. Chem Eng J 448:137726. https:\/\/doi.org\/10.1016\/j.cej.2022.137726","journal-title":"Chem Eng J"},{"key":"3371_CR11","doi-asserted-by":"publisher","first-page":"1530","DOI":"10.1007\/s11814-020-0579-1","volume":"37","author":"SP de Oliveira","year":"2020","unstructured":"de Oliveira SP, Alvarez Rodrigues N, Casciatori-Frassatto PA, Casciatori FP (2020) Solid-liquid extraction of cellulases from fungal solid-state cultivation in a packed bed bioreactor. Korean J Chem Eng 37:1530\u20131540. https:\/\/doi.org\/10.1007\/s11814-020-0579-1","journal-title":"Korean J Chem Eng"},{"key":"3371_CR12","doi-asserted-by":"publisher","first-page":"51","DOI":"10.55815\/424209","volume":"81","author":"A S\u00e1nchez","year":"2024","unstructured":"S\u00e1nchez A, Oiza N, Artola A, Font X, Barrena R, Moral-Vico J, Gea T (2024) Solid-state fermentation: A review of its opportunities and challenges in the framework of circular bioeconomy. Afinidad 81:51\u201357. https:\/\/doi.org\/10.55815\/424209","journal-title":"Afinidad"},{"key":"3371_CR13","doi-asserted-by":"publisher","first-page":"1536","DOI":"10.1590\/S1517-83822012000400038","volume":"43","author":"AI Zanelato","year":"2012","unstructured":"Zanelato AI, Shiota VM, Gomes E, da Silva R, Thom\u00e9o JC (2012) Endoglucanase production with the newly isolated Myceliophtora sp. I-1D3b in a packed bed solid state fermentor. Braz J Microbiol 43:1536\u20131544. https:\/\/doi.org\/10.1590\/S1517-83822012000400038","journal-title":"Braz J Microbiol"},{"key":"3371_CR14","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/j.cej.2014.06.040","volume":"255","author":"FP Casciatori","year":"2014","unstructured":"Casciatori FP, Laurentino CL, Taboga SR, Casciatori PA, Thom\u00e9o JC (2014) Structural properties of beds packed with agro-industrial solid by-products applicable for solid-state fermentation: Experimental data and effects on process performance. Chem Eng J 255:214\u2013224. https:\/\/doi.org\/10.1016\/j.cej.2014.06.040","journal-title":"Chem Eng J"},{"key":"3371_CR15","doi-asserted-by":"publisher","first-page":"227","DOI":"10.3390\/biom10020227","volume":"10","author":"C Favaro","year":"2020","unstructured":"Favaro C, Baraldi I, Casciatori FP, Farinas CS (2020) \u03b2-Mannanase production using coffee industry waste for application in soluble coffee processing. Biomolecules 10:227. https:\/\/doi.org\/10.3390\/biom10020227","journal-title":"Biomolecules"},{"key":"3371_CR16","first-page":"271","volume":"39","author":"G Viccini","year":"2001","unstructured":"Viccini G, Mitchell DA, Boit SD, Gern JCC, da Rosa AS, Costa RM, Dalsenter FDH, von Meien OF, Krieger N (2001) Analysis of growth kinetic profiles in solid-state fermentation. Food Technol Biotechnol 39:271\u2013294","journal-title":"Food Technol Biotechnol"},{"key":"3371_CR17","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.cej.2015.10.108","volume":"287","author":"FP Casciatori","year":"2016","unstructured":"Casciatori FP, B\u00fcck A, Thom\u00e9o JC, Tsotsas E (2016) Two-phase and two-dimensional model describing heat and water transfer during solid-state fermentation within a packed-bed bioreactor. Chem Eng J 287:103\u2013116. https:\/\/doi.org\/10.1016\/j.cej.2015.10.108","journal-title":"Chem Eng J"},{"key":"3371_CR18","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1007\/s11274-022-03240-5","volume":"38","author":"LCS Corr\u00eaa Junior","year":"2022","unstructured":"Corr\u00eaa Junior LCS, Gaut\u00e9rio GV, de Medeiros Burkert JF, Kalil SJ (2022) Purification of xylanases from Aureobasidium pullulans CCT 1261 and its application in the production of xylooligosaccharides. World J Microbiol Biotechnol 38:52. https:\/\/doi.org\/10.1007\/s11274-022-03240-5","journal-title":"World J Microbiol Biotechnol"},{"key":"3371_CR19","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/S0032-9592(99)00064-3","volume":"35","author":"EV Cortez","year":"1999","unstructured":"Cortez EV, Pessoa A (1999) Xylanase and \u03b2-xylosidase separation by fractional precipitation. Process Biochem 35:277\u2013283. https:\/\/doi.org\/10.1016\/S0032-9592(99)00064-3","journal-title":"Process Biochem"},{"key":"3371_CR20","first-page":"259","volume-title":"Handbook of Biochemistry and Molecular Biology","author":"RL Lundblad","year":"2018","unstructured":"Lundblad RL, Macdonald F (2018) Proteases in the interstitial space. Handbook of Biochemistry and Molecular Biology. CRC, Boca Raton, pp 259\u2013266"},{"key":"3371_CR21","doi-asserted-by":"publisher","first-page":"462","DOI":"10.3390\/fermentation9050462","volume":"9","author":"W Zhou","year":"2023","unstructured":"Zhou W, Tong S, Amin FR, Chen W, Cai J, Li D (2023) Heterologous expression and biochemical characterization of a thermostable endoglucanase (MtEG5-1) from Myceliophthora thermophila. Fermentation 9:462. https:\/\/doi.org\/10.3390\/fermentation9050462","journal-title":"Fermentation"},{"key":"3371_CR22","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1002\/bit.260300604","volume":"30","author":"A Sadana","year":"1987","unstructured":"Sadana A, Henley JP (1987) Single-step unimolecular non\u2010first\u2010order enzyme deactivation kinetics. Biotechnol Bioeng 30:717\u2013723. https:\/\/doi.org\/10.1002\/bit.260300604","journal-title":"Biotechnol Bioeng"},{"key":"3371_CR23","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1351\/pac198759020257","volume":"59","author":"TK Ghose","year":"1987","unstructured":"Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59:257\u2013268. https:\/\/doi.org\/10.1351\/pac198759020257","journal-title":"Pure Appl Chem"},{"key":"3371_CR24","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1021\/ac60147a030","volume":"31","author":"GL Miller","year":"1959","unstructured":"Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426\u2013428. https:\/\/doi.org\/10.1021\/ac60147a030","journal-title":"Anal Chem"},{"key":"3371_CR25","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.bej.2009.10.008","volume":"48","author":"DA Mitchell","year":"2010","unstructured":"Mitchell DA, Cunha LEN, Machado AVL, de Lima Luz LF, Krieger N (2010) A model-based investigation of the potential advantages of multi-layer packed beds in solid-state fermentation. Biochem Eng J 48:195\u2013203. https:\/\/doi.org\/10.1016\/j.bej.2009.10.008","journal-title":"Biochem Eng J"},{"key":"3371_CR26","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1007\/s00449-020-02466-7","volume":"44","author":"CL Perez","year":"2021","unstructured":"Perez CL, Casciatori FP, Thom\u00e9o JC (2021) Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution. Bioprocess Biosyst Eng 44:537\u2013548. https:\/\/doi.org\/10.1007\/s00449-020-02466-7","journal-title":"Bioprocess Biosyst Eng"},{"key":"3371_CR27","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/0141-0229(94)90051-5","volume":"16","author":"NP Ghildyal","year":"1994","unstructured":"Ghildyal NP, Gowthaman MK, Raghava Rao KSMS, Karanth NG (1994) Interaction of transport resistances with biochemical reaction in packed-bed solid-state fermentors: Effect of temperature gradients. Enzyme Microb Technol 16:253\u2013257. https:\/\/doi.org\/10.1016\/0141-0229(94)90051-5","journal-title":"Enzyme Microb Technol"},{"key":"3371_CR28","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/S1369-703X(02)00122-5","volume":"13","author":"P Gervais","year":"2003","unstructured":"Gervais P, Molin P (2003) The role of water in solid-state fermentation. Biochem Eng J 13:85\u2013101. https:\/\/doi.org\/10.1016\/S1369-703X(02)00122-5","journal-title":"Biochem Eng J"},{"key":"3371_CR29","doi-asserted-by":"publisher","first-page":"9121","DOI":"10.1007\/s13399-022-02977-1","volume":"14","author":"E Katayama","year":"2022","unstructured":"Katayama E, Rodrigues NA, Bilatto S, Casciatori FP, Farinas CS (2022) Nanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor. Biomass Convers Biorefin 14:9121\u20139136. https:\/\/doi.org\/10.1007\/s13399-022-02977-1","journal-title":"Biomass Convers Biorefin"},{"key":"3371_CR30","doi-asserted-by":"publisher","first-page":"1142","DOI":"10.1016\/j.cej.2018.12.169","volume":"361","author":"CL Perez","year":"2019","unstructured":"Perez CL, Casciatori FP, Thom\u00e9o JC (2019) Strategies for scaling-up packed-bed bioreactors for solid-state fermentation: The case of cellulolytic enzymes production by a thermophilic fungus. Chem Eng J 361:1142\u20131151. https:\/\/doi.org\/10.1016\/j.cej.2018.12.169","journal-title":"Chem Eng J"},{"key":"3371_CR31","doi-asserted-by":"publisher","first-page":"1186","DOI":"10.1016\/j.indcrop.2011.04.006","volume":"34","author":"CS Farinas","year":"2011","unstructured":"Farinas CS, Vitcosque GL, Fonseca RF, Neto VB, Couri S (2011) Modeling the effects of solid state fermentation operating conditions on endoglucanase production using an instrumented bioreactor. Ind Crops Prod 34:1186\u20131192. https:\/\/doi.org\/10.1016\/j.indcrop.2011.04.006","journal-title":"Ind Crops Prod"},{"key":"3371_CR32","doi-asserted-by":"publisher","first-page":"117793","DOI":"10.1016\/j.ces.2022.117793","volume":"259","author":"JP Henrique","year":"2022","unstructured":"Henrique JP, Casciatori FP, Thom\u00e9o JC (2022) Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor. Chem Eng Sci 259:117793. https:\/\/doi.org\/10.1016\/j.ces.2022.117793","journal-title":"Chem Eng Sci"},{"key":"3371_CR33","doi-asserted-by":"publisher","first-page":"2761","DOI":"10.1007\/s12649-018-0327-5","volume":"10","author":"M Mar\u00edn","year":"2019","unstructured":"Mar\u00edn M, Artola A, S\u00e1nchez A (2019) Optimization of down-stream for cellulases produced under solid-state fermentation of coffee husk. Waste Biomass Valor 10:2761\u20132772. https:\/\/doi.org\/10.1007\/s12649-018-0327-5","journal-title":"Waste Biomass Valor"},{"key":"3371_CR34","doi-asserted-by":"publisher","first-page":"106776","DOI":"10.1016\/j.compchemeng.2020.106776","volume":"135","author":"F Fenila","year":"2020","unstructured":"Fenila F, Shastri Y (2020) Stochastic optimization of enzymatic hydrolysis of lignocellulosic biomass. Comput Chem Eng 135:106776. https:\/\/doi.org\/10.1016\/j.compchemeng.2020.106776","journal-title":"Comput Chem Eng"},{"key":"3371_CR35","doi-asserted-by":"publisher","first-page":"111822","DOI":"10.1016\/j.rser.2021.111822","volume":"154","author":"C Huang","year":"2022","unstructured":"Huang C, Jiang X, Shen X, Hu J, Tang W, Wu X, Ragauskas A, Jameel H, Meng X, Yong Q (2022) Lignin-enzyme interaction: A roadblock for efficient enzymatic hydrolysis of lignocellulosics. Renew Sustain Energy Rev 154:111822. https:\/\/doi.org\/10.1016\/j.rser.2021.111822","journal-title":"Renew Sustain Energy Rev"},{"key":"3371_CR36","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1016\/j.renene.2017.07.062","volume":"114","author":"FB Mendes","year":"2017","unstructured":"Mendes FB, Ibraim Pires Atala D, Thom\u00e9o JC (2017) Is cellulase production by solid-state fermentation economically attractive for the second generation ethanol production? Renew Energy 114:525\u2013533. https:\/\/doi.org\/10.1016\/j.renene.2017.07.062","journal-title":"Renew Energy"},{"key":"3371_CR37","doi-asserted-by":"publisher","first-page":"1575","DOI":"10.1002\/jctb.6967","volume":"97","author":"E Caroca","year":"2022","unstructured":"Caroca E, Elorrieta M, Palma C, Navia D, Lebrero R, Carvajal A (2022) Lignocellulosic residue valorization in a sequential process of solid-state fermentation and solid substrate anaerobic digestion. J Chem Technol Biotechnol 97:1575\u20131584. https:\/\/doi.org\/10.1002\/jctb.6967","journal-title":"J Chem Technol Biotechnol"},{"key":"3371_CR38","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1016\/j.jclepro.2019.06.100","volume":"233","author":"E Catal\u00e1n","year":"2019","unstructured":"Catal\u00e1n E, Komilis D, S\u00e1nchez A (2019) Environmental impact of cellulase production from coffee husks by solid-state fermentation: A life-cycle assessment. J Clean Prod 233:954\u2013962. https:\/\/doi.org\/10.1016\/j.jclepro.2019.06.100","journal-title":"J Clean Prod"},{"key":"3371_CR39","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1186\/s13068-021-01920-2","volume":"14","author":"JC Rohrbach","year":"2021","unstructured":"Rohrbach JC, Luterbacher JS (2021) Investigating the effects of substrate morphology and experimental conditions on the enzymatic hydrolysis of lignocellulosic biomass through modeling. Biotechnol Biofuels 14:103. https:\/\/doi.org\/10.1186\/s13068-021-01920-2","journal-title":"Biotechnol Biofuels"},{"key":"3371_CR40","doi-asserted-by":"publisher","unstructured":"Santos Gomes MMO dos, Nicodemos IS, da Costa Silva M, Santos Martins TV dos, Freitas Gon\u00e7alves JMDMS de, Meneghetti SMP, Franco M, Gomes FS, Pereira HJV (2024) Re-recycling agro-industrial waste: exploiting activated carbon from cocoa shells after solid-state fermentation as a support for endoglucanase immobilization. Biomass Convers Biorefin. https:\/\/doi.org\/10.1007\/s13399-024-05748-2","DOI":"10.1007\/s13399-024-05748-2"},{"key":"3371_CR41","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1007\/s00449-021-02539-1","volume":"44","author":"S Dahiya","year":"2021","unstructured":"Dahiya S, Kumar A, Malik V, Kumar V, Singh B (2021) Biochemical characterization and enhanced production of endoxylanase from thermophilic mould Myceliophthora thermophila. Bioprocess Biosyst Eng 44:1539\u20131555. https:\/\/doi.org\/10.1007\/s00449-021-02539-1","journal-title":"Bioprocess Biosyst Eng"},{"key":"3371_CR42","doi-asserted-by":"publisher","first-page":"8441","DOI":"10.1128\/AEM.02130-12","volume":"78","author":"KK Podkaminer","year":"2012","unstructured":"Podkaminer KK, Guss AM, Trajano HL, Hogsett DA, Lynd LR (2012) Characterization of xylan utilization and discovery of a new endoxylanase in Thermoanaerobacterium saccharolyticum through Targeted Gene Deletions. Appl Environ Microbiol 78:8441\u20138447. https:\/\/doi.org\/10.1128\/AEM.02130-12","journal-title":"Appl Environ Microbiol"},{"key":"3371_CR43","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1590\/0104-6632.20150322s00003268","volume":"32","author":"MA Mari\u00f1o","year":"2015","unstructured":"Mari\u00f1o MA, Freitas S, Miranda EA (2015) Ethanol precipitation of glycosyl hydrolases produced by Trichoderma harzianum P49P11. Braz J Chem Eng 32:325\u2013333. https:\/\/doi.org\/10.1590\/0104-6632.20150322s00003268","journal-title":"Braz J Chem Eng"},{"key":"3371_CR44","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1007\/s10123-023-00322-8","volume":"26","author":"Y Qin","year":"2023","unstructured":"Qin Y, Qin B, Zhang J, Fu Y, Li Q, Luo F, Luo Y, He H (2023) Purification and enzymatic properties of a new thermostable endoglucanase from Aspergillus oryzae HML366. Int Microbiol 26:579\u2013589. https:\/\/doi.org\/10.1007\/s10123-023-00322-8","journal-title":"Int Microbiol"},{"key":"3371_CR45","doi-asserted-by":"publisher","first-page":"103483","DOI":"10.1016\/j.sjbs.2022.103483","volume":"29","author":"K Nisar","year":"2022","unstructured":"Nisar K, Abdullah R, Kaleem A, Iqtedar M, Aftab M, Saleem F (2022) Purification, characterization and thermodynamic analysis of cellulases produced from Thermomyces dupontii and its industrial applications. Saudi J Biol Sci 29:103483. https:\/\/doi.org\/10.1016\/j.sjbs.2022.103483","journal-title":"Saudi J Biol Sci"},{"key":"3371_CR46","doi-asserted-by":"publisher","first-page":"3361","DOI":"10.1016\/j.ijbiomac.2020.08.225","volume":"164","author":"C Han","year":"2020","unstructured":"Han C, Liu Y, Liu M, Wang S, Wang Q (2020) Improving the thermostability of a thermostable endoglucanase from Chaetomium thermophilum by engineering the conserved noncatalytic residue and N-glycosylation site. Int J Biol Macromol 164:3361\u20133368. https:\/\/doi.org\/10.1016\/j.ijbiomac.2020.08.225","journal-title":"Int J Biol Macromol"},{"key":"3371_CR47","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1016\/j.ijbiomac.2021.04.110","volume":"182","author":"A Patel","year":"2021","unstructured":"Patel A, Shah A (2021) Purification and characterization of novel, thermostable and non-processive GH5 family endoglucanase from Fomitopsis meliae CFA 2. Int J Biol Macromol 182:1161\u20131169. https:\/\/doi.org\/10.1016\/j.ijbiomac.2021.04.110","journal-title":"Int J Biol Macromol"},{"key":"3371_CR48","doi-asserted-by":"publisher","first-page":"2489","DOI":"10.1038\/s41598-021-81807-2","volume":"11","author":"A Koteshwara","year":"2021","unstructured":"Koteshwara A, Philip NV, Aranjani JM, Hariharapura RC, Volety Mallikarjuna S (2021) A set of simple methods for detection and extraction of laminarinase. Sci Rep 11:2489. https:\/\/doi.org\/10.1038\/s41598-021-81807-2","journal-title":"Sci Rep"},{"key":"3371_CR50","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1128\/MMBR.64.3.461-488.2000","volume":"64","author":"R Maheshwari","year":"2000","unstructured":"Maheshwari R, Bharadwaj G, Bhat MK (2000) Thermophilic fungi: Their physiology and enzymes. Microbiol Mol Biol Rev 64:461\u2013488. https:\/\/doi.org\/10.1128\/MMBR.64.3.461-488.2000","journal-title":"Microbiol Mol Biol Rev"},{"key":"3371_CR51","doi-asserted-by":"publisher","first-page":"4337","DOI":"10.33263\/BRIAC124.43374347","volume":"12","author":"ATA Abdulmajeed","year":"2021","unstructured":"Abdulmajeed ATA, \u015eahin S, Ozmen I (2021) Production and purification of the endoglucanase enzyme from local isolate Aspergillus fumigatus HBF356. Biointerface Res Appl Chem 12:4337\u20134347. https:\/\/doi.org\/10.33263\/BRIAC124.43374347","journal-title":"Biointerface Res Appl Chem"},{"key":"3371_CR52","doi-asserted-by":"publisher","first-page":"152","DOI":"10.3390\/jof9020152","volume":"9","author":"A L\u00f3pez-L\u00f3pez","year":"2023","unstructured":"L\u00f3pez-L\u00f3pez A, Santiago-Hern\u00e1ndez A, Cayetano-Cruz M, Garc\u00eda-Huante Y, Campos JE, Bustos-Jaimes I, Marsch-Moreno R, Cano-Ram\u00edrez C, Benitez-Cardoza CG, Hidalgo-Lara ME (2023) TtCel7A: A native thermophilic bifunctional cellulose\/xylanase exogluclanase from the thermophilic biomass-degrading fungus Thielavia terrestris Co3Bag1, and its application in enzymatic hydrolysis of agroindustrial derivatives. J Fungi 9:152. https:\/\/doi.org\/10.3390\/jof9020152","journal-title":"J Fungi"},{"key":"3371_CR53","doi-asserted-by":"publisher","first-page":"6849","DOI":"10.3390\/ijms26146849","volume":"26","author":"S Xu","year":"2025","unstructured":"Xu S, Ma K, Chen Z, Zhao J, Song X, Qin Y (2025) Screening, characterization and comparison of endoglucanases\/xylanases from thermophilic fungi: A Thielavia terrestris xylanase with high activity-stability properties. Int J Mol Sci 26:6849. https:\/\/doi.org\/10.3390\/ijms26146849","journal-title":"Int J Mol Sci"},{"key":"3371_CR54","doi-asserted-by":"publisher","first-page":"20474","DOI":"10.1021\/acsomega.5c00685","volume":"10","author":"VB Andrade","year":"2025","unstructured":"Andrade VB, Tramontina R, Almeida DV, Tomazetto G, da Silva VM, Gabel F, Marcello YMB, Scott AL, Squina FM, Garcia W (2025) Dissection of an anaerobic fungal cellulosomal endoglucanase: Impact of the dockerin module on activity, thermostability, and flexibility. ACS Omega 10:20474\u201320486. https:\/\/doi.org\/10.1021\/acsomega.5c00685","journal-title":"ACS Omega"},{"key":"3371_CR55","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1016\/j.myc.2016.07.003","volume":"57","author":"S Boonrung","year":"2016","unstructured":"Boonrung S, Katekaew S, Mongkolthanaruk W, Aimi T, Boonlue S (2016) Purification and characterization of low molecular weight extreme alkaline xylanase from the thermophilic fungus Myceliophthora thermophila BF1-7. Mycoscience 57:408\u2013416. https:\/\/doi.org\/10.1016\/j.myc.2016.07.003","journal-title":"Mycoscience"},{"key":"3371_CR56","doi-asserted-by":"publisher","first-page":"123440","DOI":"10.1016\/j.ijbiomac.2023.123440","volume":"232","author":"NG Nezhad","year":"2023","unstructured":"Nezhad NG, Rahman RNZRA, Normi YM, Oslan SN, Shariff FM, Leow TC (2023) Recent advances in simultaneous thermostability-activity improvement of industrial enzymes through structure modification. Int J Biol Macromol 232:123440. https:\/\/doi.org\/10.1016\/j.ijbiomac.2023.123440","journal-title":"Int J Biol Macromol"},{"key":"3371_CR57","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1186\/s13068-017-0813-1","volume":"10","author":"A Karnaouri","year":"2017","unstructured":"Karnaouri A, Muraleedharan MN, Dimarogona M, Topakas E, Rova U, Sandgren M, Christakopoulos P (2017) Recombinant expression of thermostable processive MtEG5 endoglucanase and its synergism with MtLPMO from Myceliophthora thermophila during the hydrolysis of lignocellulosic substrates. Biotechnol Biofuels 10:126. https:\/\/doi.org\/10.1186\/s13068-017-0813-1","journal-title":"Biotechnol Biofuels"},{"key":"3371_CR58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1042\/bse0590001","volume":"59","author":"PK Robinson","year":"2015","unstructured":"Robinson PK (2015) Enzymes: principles and biotechnological applications. Essays Biochem 59:1\u201341. https:\/\/doi.org\/10.1042\/bse0590001","journal-title":"Essays Biochem"},{"key":"3371_CR59","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.1007\/s00449-019-02113-w","volume":"42","author":"J de Souza Lima","year":"2019","unstructured":"de Souza Lima J, Costa FN, Bastistella MA, de Ara\u00fajo PHH, de Oliveira D (2019) Functionalized kaolin as support for endoglucanase immobilization. Bioprocess Biosyst Eng 42:1165\u20131173. https:\/\/doi.org\/10.1007\/s00449-019-02113-w","journal-title":"Bioprocess Biosyst Eng"},{"key":"3371_CR60","doi-asserted-by":"publisher","unstructured":"Silva MBP, de Abdal O, Prado D, Dias JPZ, de Morales G, Xavier SAV, Almeida M, de Silva AF, Maiorano ES, Perna AE (2021) RF Effect of temperature, pH and storage time on the stability of an extracellular fructosyltransferase from Aspergillus oryzae IPT-301. Braz J Food Technol 24:e2020283. https:\/\/doi.org\/10.1590\/1981-6723.28320","DOI":"10.1590\/1981-6723.28320"},{"key":"3371_CR61","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/0141-0229(86)90088-8","volume":"8","author":"F Stutzenberger","year":"1986","unstructured":"Stutzenberger F, Lupo D (1986) pH-dependent thermal activation of endo-1,4-\u03b2-glucanase in Thermomonospora curvata. Enzyme Microb Technol 8:205\u2013208. https:\/\/doi.org\/10.1016\/0141-0229(86)90088-8","journal-title":"Enzyme Microb Technol"},{"key":"3371_CR62","doi-asserted-by":"publisher","first-page":"10","DOI":"10.54963\/neea.v1i2.42","volume":"1","author":"B Singh","year":"2022","unstructured":"Singh B, Anu A, Singh D, Kumar V, Kumar V, Malik V (2022) Cellulase production by Myceliophthora thermophila in solid state fermentation and its utility in saccharification of rice straw. New Energy Exploit Appl 1:10\u201317. https:\/\/doi.org\/10.54963\/neea.v1i2.42","journal-title":"New Energy Exploit Appl"}],"container-title":["Bioprocess and Biosystems Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00449-026-03371-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00449-026-03371-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00449-026-03371-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T05:29:57Z","timestamp":1784266197000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00449-026-03371-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,29]]},"references-count":61,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["3371"],"URL":"https:\/\/doi.org\/10.1007\/s00449-026-03371-1","relation":{},"ISSN":["1615-7591","1615-7605"],"issn-type":[{"value":"1615-7591","type":"print"},{"value":"1615-7605","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,29]]},"assertion":[{"value":"16 April 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 June 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":3,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}]}}