{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:54:10Z","timestamp":1791824050308,"version":"4.3.4"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.485351\/2020-00"],"award-info":[{"award-number":["88887.485351\/2020-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/13603-3"],"award-info":[{"award-number":["2016\/13603-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Bioprocess Biosyst Eng"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Biological sulfate reduction combined with chemical precipitation of metal sulfides is an good alternative to mitigate environmental impacts of acid mine drainage (AMD) and to recover base metals. This process requires a consistent supply of electron donors, and this study investigated the use of Brewery Spent Grain (BSG) as a slow-release electron donor (SRED) to sustain sulfate reduction and enhance metal sulfide precipitation as settling particles. A microbial consortium was enriched in batch reactors using poultry manure as the primary inoculum to evaluate its performance, metabolic pathways, and resilience to sulfide toxicity. Consortium enrichment successfully promoted a synergistic consortium where fermentative bacteria, notably\n                    <jats:italic>Macellibacteroides<\/jats:italic>\n                    and\n                    <jats:italic>Enterococcus<\/jats:italic>\n                    , hydrolyzed the lignocellulosic BSG to release lactate, hydrogen, propionate, and butyrate. These substrates were subsequently utilized by SRB groups, including\n                    <jats:italic>Bacteroides graminisolvens<\/jats:italic>\n                    ,\n                    <jats:italic>Desulforhabdus<\/jats:italic>\n                    sp., and\n                    <jats:italic>Desulfomicrobium<\/jats:italic>\n                    , to drive sulfate reduction. The enrichment process increased the sulfide concentration and the sulfate removal rate. Optimization assays revealed that a ratio of 8\u00a0g BSG\/g SO\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>2\u2212<\/jats:sup>\n                    provided the best conditions for sulfate removal. Conversely, excess BSG led to reactor acidification, slowing performance and causing excessive gas production. Sulfide toxicity tests demonstrated that while high sulfide accumulation may increase the lag phase, it does not significantly interfere with the ultimate sulfate consumption rate. The sum of those results provides optimized parameters for biogenic sulfide generation using BSG, giving a new perspective on the integration of industrial by-products into circular AMD remediation, establishing a foundation for more sustainable mining practices aligned with future global demands.\n                  <\/jats:p>","DOI":"10.1007\/s00449-026-03440-5","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:33:39Z","timestamp":1791822819000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fundamental aspects of lignocellulosic material as slow-release electron donor for biological sulfate reduction"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2760-3465","authenticated-orcid":false,"suffix":"Jr.","given":"Raul","family":"Natale","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9914-7330","authenticated-orcid":false,"given":"Rachel B.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1705-0763","authenticated-orcid":false,"given":"Tiago P.","family":"Delforno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1810-5313","authenticated-orcid":false,"given":"Kelly J.","family":"Dussan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5590-2907","authenticated-orcid":false,"given":"Guilherme Salvador","family":"Peres Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8126-5702","authenticated-orcid":false,"given":"Denise","family":"Bevilaqua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"3440_CR1","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1016\/j.jenvman.2017.04.027","volume":"197","author":"LAG de Godoi","year":"2017","unstructured":"de Godoi LAG, Foresti E, Damianovic MHRZ (2017) Down-flow fixed-structured bed reactor: an innovative reactor configuration applied to acid mine drainage treatment and metal recovery. J Environ Manage 197:597\u2013604. https:\/\/doi.org\/10.1016\/j.jenvman.2017.04.027","journal-title":"J Environ Manage"},{"key":"3440_CR2","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.jhazmat.2013.12.032","volume":"269","author":"I S\u00e1nchez-Andrea","year":"2014","unstructured":"S\u00e1nchez-Andrea I, Sanz JL, Bijmans MFM, Stams AJM (2014) Sulfate reduction at low pH to remediate acid mine drainage. J Hazard Mater 269:98\u2013109. https:\/\/doi.org\/10.1016\/j.jhazmat.2013.12.032","journal-title":"J Hazard Mater"},{"key":"3440_CR3","doi-asserted-by":"publisher","first-page":"118462","DOI":"10.1016\/j.jenvman.2023.118462","volume":"344","author":"X Zhang","year":"2023","unstructured":"Zhang X, Zeng L, Wang Y et al (2023) Selective separation of metals from wastewater using sulfide precipitation: a critical review in agents, operational factors and particle aggregation. J Environ Manage 344:118462. https:\/\/doi.org\/10.1016\/j.jenvman.2023.118462","journal-title":"J Environ Manage"},{"key":"3440_CR4","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1002\/elsc.200720216","volume":"7","author":"AH Kaksonen","year":"2007","unstructured":"Kaksonen AH, Puhakka JA (2007) Sulfate reduction based bioprocesses for the treatment of acid mine drainage and the recovery of metals. Eng Life Sci 7:541\u2013564. https:\/\/doi.org\/10.1002\/elsc.200720216","journal-title":"Eng Life Sci"},{"key":"3440_CR5","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1002\/ep.670010306","volume":"1","author":"JS Whang","year":"1982","unstructured":"Whang JS, Young D, Pressman M (1982) Soluble-sulfide precipitation for heavy metals removal from wastewaters. Environ Prog 1:110\u2013113. https:\/\/doi.org\/10.1002\/ep.670010306","journal-title":"Environ Prog"},{"key":"3440_CR6","doi-asserted-by":"publisher","first-page":"123622","DOI":"10.1016\/j.jhazmat.2020.123622","volume":"403","author":"RB Costa","year":"2021","unstructured":"Costa RB, Godoi LAG, Braga AFM et al (2021) Sulfate removal rate and metal recovery as settling precipitates in bioreactors: influence of electron donors. J Hazard Mater 403:123622. https:\/\/doi.org\/10.1016\/j.jhazmat.2020.123622","journal-title":"J Hazard Mater"},{"key":"3440_CR7","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.psep.2024.01.103","volume":"184","author":"S Bagheri Novair","year":"2024","unstructured":"Bagheri Novair S, Biglari Quchan Atigh Z, Asgari Lajayer B et al (2024) The role of sulphate-reducing bacteria (SRB) in bioremediation of sulphate-rich wastewater: focus on the source of electron donors. Process Saf Environ Prot 184:190\u2013207","journal-title":"Process Saf Environ Prot"},{"key":"3440_CR8","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.minpro.2012.02.005","volume":"106\u2013109","author":"J Cao","year":"2012","unstructured":"Cao J, Zhang G, Mao ZS et al (2012) Influence of electron donors on the growth and activity of sulfate-reducing bacteria. Int J Min Process 106\u2013109:58\u201364. https:\/\/doi.org\/10.1016\/j.minpro.2012.02.005","journal-title":"Int J Min Process"},{"key":"3440_CR9","doi-asserted-by":"publisher","first-page":"5115","DOI":"10.1007\/s11356-017-9334-5","volume":"25","author":"LC Reyes-Alvarado","year":"2018","unstructured":"Reyes-Alvarado LC, Camarillo-Gamboa \u00c1, Rustrian E et al (2018) Lignocellulosic biowastes as carrier material and slow release electron donor for sulphidogenesis of wastewater in an inverse fluidized bed bioreactor. Environ Sci Pollut Res 25:5115\u20135128. https:\/\/doi.org\/10.1007\/s11356-017-9334-5","journal-title":"Environ Sci Pollut Res"},{"key":"3440_CR10","doi-asserted-by":"publisher","first-page":"1972","DOI":"10.1021\/es9703335","volume":"32","author":"KR Waybrant","year":"1998","unstructured":"Waybrant KR, Blowes DW, Ptacek CJ (1998) Selection of reactive mixtures for use in permeable reactive walls for treatment of mine drainage. Environ Sci Technol 32:1972\u20131979. https:\/\/doi.org\/10.1021\/es9703335","journal-title":"Environ Sci Technol"},{"key":"3440_CR11","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.hydromet.2005.12.009","volume":"81","author":"A Lewis","year":"2006","unstructured":"Lewis A, Van Hille R (2006) An exploration into the sulphide precipitation method and its effect on metal sulphide removal. Hydrometallurgy 81:197\u2013204. https:\/\/doi.org\/10.1016\/j.hydromet.2005.12.009","journal-title":"Hydrometallurgy"},{"key":"3440_CR12","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.jhazmat.2011.05.002","volume":"192","author":"D Villa-Gomez","year":"2011","unstructured":"Villa-Gomez D, Ababneh H, Papirio S et al (2011) Effect of sulfide concentration on the location of the metal precipitates in inversed fluidized bed reactors. J Hazard Mater 192:200\u2013207. https:\/\/doi.org\/10.1016\/j.jhazmat.2011.05.002","journal-title":"J Hazard Mater"},{"key":"3440_CR13","doi-asserted-by":"publisher","first-page":"111216","DOI":"10.1016\/j.jenvman.2020.111216","volume":"275","author":"RB Costa","year":"2020","unstructured":"Costa RB, Bevilaqua D, Lens PNL (2020) Pre-treatment and temperature effects on the use of slow release electron donor for biological sulfate reduction. J Environ Manage 275:111216. https:\/\/doi.org\/10.1016\/j.jenvman.2020.111216","journal-title":"J Environ Manage"},{"key":"3440_CR14","doi-asserted-by":"publisher","unstructured":"Garcia-Ochoa F, Vergara P, Wojtusik M et al (2021) Multi-feedstock lignocellulosic biorefineries based on biological processes: an overview. Ind Crops Prod 172:114062 https:\/\/doi.org\/10.1016\/j.indcrop.2021.114062","DOI":"10.1016\/j.indcrop.2021.114062"},{"key":"3440_CR15","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/j.biortech.2013.08.046","volume":"148","author":"SI Mussatto","year":"2013","unstructured":"Mussatto SI, Moncada J, Roberto IC, Cardona CA (2013) Techno-economic analysis for brewer\u2019s spent grains use on a biorefinery concept: the Brazilian case. Bioresour Technol 148:302\u2013310. https:\/\/doi.org\/10.1016\/j.biortech.2013.08.046","journal-title":"Bioresour Technol"},{"key":"3440_CR16","doi-asserted-by":"publisher","unstructured":"Sun S, Gu X, Zhang M et al (2021) Response mechanism of different electron donors for treating secondary effluent in ecological floating bed. Bioresour Technol. https:\/\/doi.org\/10.1016\/j.biortech.2021.125083","DOI":"10.1016\/j.biortech.2021.125083"},{"key":"3440_CR17","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1007\/s00203-019-01625-z","volume":"201","author":"I Kushkevych","year":"2019","unstructured":"Kushkevych I, Dordevi\u0107 D, V\u00edt\u011bzov\u00e1 M (2019) Toxicity of hydrogen sulfide toward sulfate-reducing bacteria Desulfovibrio piger Vib-7. Arch Microbiol 201:389\u2013397. https:\/\/doi.org\/10.1007\/s00203-019-01625-z","journal-title":"Arch Microbiol"},{"key":"3440_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-018-1101-4","volume":"11","author":"CO Nzeteu","year":"2018","unstructured":"Nzeteu CO, Trego AC, Abram F, O\u2019Flaherty V (2018) Reproducible, high-yielding, biological caproate production from food waste using a single-phase anaerobic reactor system. Biotechnol Biofuels 11:1\u201314. https:\/\/doi.org\/10.1186\/s13068-018-1101-4","journal-title":"Biotechnol Biofuels"},{"key":"3440_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fbioe.2020.550253","volume":"8","author":"AP Florentino","year":"2020","unstructured":"Florentino AP, Costa RB, Hu Y et al (2020) Long chain fatty acid degradation coupled to biological sulfidogenesis: a prospect for enhanced metal recovery. Front Bioeng Biotechnol 8:1\u201314. https:\/\/doi.org\/10.3389\/fbioe.2020.550253","journal-title":"Front Bioeng Biotechnol"},{"key":"3440_CR20","doi-asserted-by":"publisher","first-page":"1114","DOI":"10.1128\/aem.59.4.1114-1119.1993","volume":"59","author":"AJM Stams","year":"1993","unstructured":"Stams AJM, Van Dijk JB, Dijkema C, Plugge CM (1993) Growth of syntrophic propionate-oxidizing bacteria with fumarate in the absence of methanogenic bacteria. Appl Environ Microbiol 59:1114\u20131119. https:\/\/doi.org\/10.1128\/aem.59.4.1114-1119.1993","journal-title":"Appl Environ Microbiol"},{"key":"3440_CR21","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1007\/s002480000087","volume":"41","author":"MK Chelius","year":"2001","unstructured":"Chelius MK, Triplett EW (2001) The diversity of archaea and bacteria in association with the roots of Zea mays L. Microb Ecol 41:252\u2013263. https:\/\/doi.org\/10.1007\/s002480000087","journal-title":"Microb Ecol"},{"key":"3440_CR22","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/s13199-009-0049-3","volume":"49","author":"BP Hodkinson","year":"2009","unstructured":"Hodkinson BP, Lutzoni F (2009) A microbiotic survey of lichen-associated bacteria reveals a new lineage from the Rhizobiales. Symbiosis 49:163\u2013180. https:\/\/doi.org\/10.1007\/s13199-009-0049-3","journal-title":"Symbiosis"},{"key":"3440_CR23","unstructured":"Andrews S (2010) FastQC: a quality control tool for high throughput sequence data. In: FastQC: a quality control tool for high throughput sequence data. https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/. Accessed 10 Aug 2022"},{"key":"3440_CR24","doi-asserted-by":"publisher","first-page":"10","DOI":"10.14806\/ej.17.1.200","volume":"17","author":"M Martin","year":"2011","unstructured":"Martin M (2011) Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J 17:10. https:\/\/doi.org\/10.14806\/ej.17.1.200","journal-title":"EMBnet J"},{"key":"3440_CR25","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","volume":"13","author":"BJ Callahan","year":"2016","unstructured":"Callahan BJ, McMurdie PJ, Rosen MJ et al (2016) DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods 13:581\u2013583. https:\/\/doi.org\/10.1038\/nmeth.3869","journal-title":"Nat Methods"},{"key":"3440_CR26","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","volume":"41","author":"C Quast","year":"2012","unstructured":"Quast C, Pruesse E, Yilmaz P et al (2012) The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41:D590\u2013D596. https:\/\/doi.org\/10.1093\/nar\/gks1219","journal-title":"Nucleic Acids Res"},{"key":"3440_CR27","doi-asserted-by":"publisher","first-page":"e61217","DOI":"10.1371\/journal.pone.0061217","volume":"8","author":"PJ McMurdie","year":"2013","unstructured":"McMurdie PJ, Holmes S (2013) phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS ONE 8:e61217. https:\/\/doi.org\/10.1371\/journal.pone.0061217","journal-title":"PLoS ONE"},{"key":"3440_CR28","first-page":"265","volume":"11","author":"A Chao","year":"1984","unstructured":"Chao A (1984) Nonparametric estimation of the number of classes in a population. Scand J Stat 11:265\u2013270","journal-title":"Scand J Stat"},{"key":"3440_CR29","volume-title":"Ecological diversity and its measurement","author":"AE Magurran","year":"2013","unstructured":"Magurran AE (2013) Ecological diversity and its measurement. Springer Netherlands, Dordrecht"},{"key":"3440_CR30","doi-asserted-by":"publisher","first-page":"454","DOI":"10.4319\/lo.1969.14.3.0454","volume":"14","author":"JD Cline","year":"1969","unstructured":"Cline JD (1969) Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 14:454\u2013458. https:\/\/doi.org\/10.4319\/lo.1969.14.3.0454","journal-title":"Limnol Oceanogr"},{"key":"3440_CR31","unstructured":"ASTM (2002) Standard test method for sulfate ion in water (D516-02). West Conshohocken, PA"},{"key":"3440_CR32","unstructured":"U.S. EPA (1986) Method 9038: sulfate (turbidimetric). Washington, DC"},{"key":"3440_CR33","doi-asserted-by":"publisher","unstructured":"APHA (2012) Standard methods for the examination of water and wastewater, 22st edn. American Water Works Association\/American Public Works Association\/Water Environment Federation. https:\/\/doi.org\/10.2105\/AJPH.51.6.940-a","DOI":"10.2105\/AJPH.51.6.940-a"},{"key":"3440_CR34","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1021\/ac60111a017","volume":"28","author":"KA DuBois Michel, Gilles","year":"1956","unstructured":"DuBois Michel, Gilles KA, Hamilton JK et al (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350\u2013356. https:\/\/doi.org\/10.1021\/ac60111a017","journal-title":"Anal Chem"},{"key":"3440_CR35","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.ijhydene.2012.10.022","volume":"38","author":"V Perna","year":"2013","unstructured":"Perna V, Castell\u00f3 E, Wenzel J et al (2013) Hydrogen production in an upflow anaerobic packed bed reactor used to treat cheese whey. Int J Hydrogen Energy 38:54\u201362. https:\/\/doi.org\/10.1016\/j.ijhydene.2012.10.022","journal-title":"Int J Hydrogen Energy"},{"key":"3440_CR36","doi-asserted-by":"publisher","first-page":"4186","DOI":"10.1016\/j.watres.2004.06.023","volume":"38","author":"O Gibert","year":"2004","unstructured":"Gibert O, De Pablo J, Luis Cortina J, Ayora C (2004) Chemical characterisation of natural organic substrates for biological mitigation of acid mine drainage. Water Res 38:4186\u20134196. https:\/\/doi.org\/10.1016\/j.watres.2004.06.023","journal-title":"Water Res"},{"key":"3440_CR37","doi-asserted-by":"publisher","unstructured":"Gomes MM, Sakamoto IK, Silva Rabelo CAB et al (2021) Statistical optimization of methane production from brewery spent grain: interaction effects of temperature and substrate concentration. J Environ Manage. https:\/\/doi.org\/10.1016\/j.jenvman.2021.112363","DOI":"10.1016\/j.jenvman.2021.112363"},{"key":"3440_CR38","doi-asserted-by":"crossref","unstructured":"Schlegel HG, Jannasch HW (2006) In: Dworkin M, Falkow S, Rosenberg E et al (eds) Prokaryotes and their habitats BT - the prokaryotes: volume 1: symbiotic associations, biotechnology, applied microbiology. Springer New York, New York, pp 137\u2013184","DOI":"10.1007\/0-387-30741-9_6"},{"key":"3440_CR39","doi-asserted-by":"publisher","DOI":"10.1007\/s12649-025-03296-y","author":"KU Devens","year":"2025","unstructured":"Devens KU, de Freitas Junior JA, Ribeiro AR et al (2025) Biohydrogen production and value-added product recovery from co-digestion of cassava wastewater and glycerol in an anaerobic fluidized bed reactor. Waste Biomass Valorization. https:\/\/doi.org\/10.1007\/s12649-025-03296-y","journal-title":"Waste Biomass Valorization"},{"key":"3440_CR40","doi-asserted-by":"crossref","unstructured":"Boone DR, Castenholz RW, Garrity GM (2001) Bergey\u2019s manual of systematic bacteriology -, vol 1. The Archaea and the Deeply Branching and Phototrophic Bacteria","DOI":"10.1007\/978-0-387-21609-6"},{"key":"3440_CR41","doi-asserted-by":"publisher","first-page":"2522","DOI":"10.1099\/ijs.0.032508-0","volume":"62","author":"L Jabari","year":"2012","unstructured":"Jabari L, Gannoun H, Cayol JL et al (2012) Macellibacteroides fermentans gen. nov., sp. nov., a member of the family Porphyromonadaceae isolated from an upflow anaerobic filter treating abattoir wastewaters. Int J Syst Evol Microbiol 62:2522\u20132527. https:\/\/doi.org\/10.1099\/ijs.0.032508-0","journal-title":"Int J Syst Evol Microbiol"},{"key":"3440_CR42","doi-asserted-by":"crossref","unstructured":"Brenner DJ, Krieg NR, Staley JT et al (2005) Bergey\u2019s manual of systematic bacteriology: the proteobacteria, the gammaproteobacteria. Springer","DOI":"10.1007\/0-387-28022-7"},{"key":"3440_CR43","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1099\/ijs.0.008268-0","volume":"59","author":"T Nishiyama","year":"2009","unstructured":"Nishiyama T, Ueki A, Kaku N et al (2009) Bacteroides graminisolvens sp. nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste. Int J Syst Evol Microbiol 59:1901\u20131907. https:\/\/doi.org\/10.1099\/ijs.0.008268-0","journal-title":"Int J Syst Evol Microbiol"},{"key":"3440_CR44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmars.2014.00064","volume":"1","author":"M Holmer","year":"2014","unstructured":"Holmer M, Hasler-Sheetal H (2014) Sulfide intrusion in seagrasses assessed by stable sulfur isotopes-A synthesis of current results. Front Mar Sci 1:1\u201312. https:\/\/doi.org\/10.3389\/fmars.2014.00064","journal-title":"Front Mar Sci"},{"key":"3440_CR45","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.jhazmat.2006.07.073","volume":"140","author":"S Azabou","year":"2007","unstructured":"Azabou S, Mechichi T, Patel BKC, Sayadi S (2007) Isolation and characterization of a mesophilic heavy-metals-tolerant sulfate-reducing bacterium Desulfomicrobium sp. from an enrichment culture using phosphogypsum as a sulfate source. J Hazard Mater 140:264\u2013270. https:\/\/doi.org\/10.1016\/j.jhazmat.2006.07.073","journal-title":"J Hazard Mater"},{"key":"3440_CR46","volume-title":"Bergey\u2019s manual of systematic bacteriology - Vol 2: the proteobacteria part B -the gammaproteobacteria","author":"DJ Brenner","year":"2010","unstructured":"Brenner DJ, Krieg NR, Staley JT (2010) Bergey\u2019s manual of systematic bacteriology - Vol 2: the proteobacteria part B -the gammaproteobacteria. Springer, New York Inc"},{"key":"3440_CR47","doi-asserted-by":"publisher","unstructured":"Moloantoa K, Khetsha Z, Mochane M et al (2023) Evaluating the effects of pH and temperature on sulphate-reducing bacteria and modelling of their effects in stirred bioreactors. Environ Pollutants Bioavailab. https:\/\/doi.org\/10.1080\/26395940.2023.2257388","DOI":"10.1080\/26395940.2023.2257388"},{"key":"3440_CR48","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1080\/09593338909384801","volume":"10","author":"JA Oleszkiewicz","year":"1989","unstructured":"Oleszkiewicz JA, Marstaller T, McCartney DM (1989) Effects of pH on sulfide toxicity to anaerobic processes. Environ Technol Lett 10:815\u2013822. https:\/\/doi.org\/10.1080\/09593338909384801","journal-title":"Environ Technol Lett"}],"container-title":["Bioprocess and Biosystems Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00449-026-03440-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00449-026-03440-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00449-026-03440-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:33:41Z","timestamp":1791822821000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00449-026-03440-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,12]]},"references-count":48,"alternative-id":["3440"],"URL":"https:\/\/doi.org\/10.1007\/s00449-026-03440-5","relation":{},"ISSN":["1615-7591","1615-7605"],"issn-type":[{"value":"1615-7591","type":"print"},{"value":"1615-7605","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,12]]},"assertion":[{"value":"25 July 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}