{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:17:56Z","timestamp":1781014676682,"version":"3.54.1"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1-2","license":[{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100005788","name":"Medical University of Vienna","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005788","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Wien Klin Wochenschr"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Rapid identification of human herpesviruses from lesion swabs is necessary for timely initiation of antiviral treatment, especially with infections involving neonates and immunocompromised individuals. The aim of the study was to investigate the results of an in-house polymerase chain reaction (PCR) test for herpesviruses in patients with symptoms suggestive for a\u00a0herpesvirus infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>In this single center retrospective study the results of 3677 lesion swab specimens tested for human herpes simplex virus\u00a01 and\u00a02 (HSV 1 and 2) and varicella zoster virus (VZV) were analyzed in the context of data sheets giving details of the suspected diagnosis, medical history as well as the demographic data of the patients. The PCR procedures for cytomegalovirus (CMV), Epstein-Barr virus (EBV) and human herpes virus 8 (HHV-8) were applied on special occasions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the samples 3369 (91.6%) were swabs and a\u00a0minority were tissue or blood samples. Of the 3015 samples tested for HSV\u20111, HSV\u20112 and VZV concomitantly, 52.3% were positive for at least one of these viruses. Clinically distinct conditions, such as herpes zoster and varicella had a\u00a0high rate of positive PCR results, ranging from 81% to 88%, respectively. Among HSV\u20112 positive samples, 23.7% derived from human immunodeficiency virus (HIV) positive patients, in contrast to the 10.8% originating from immunocompetent patients, the difference being statistically significant (<jats:italic>p<\/jats:italic>\u202f&lt;\u20090.002). The HSV\u20112 was detected more often in women than in men.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Distinct clinical diagnoses have a\u00a0high correlation rate with positive PCR results. A\u00a0significantly higher number of HSV\u20112 positive results were found in HIV positive patients and in women.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s00508-019-01585-w","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T08:02:53Z","timestamp":1575878573000},"page":"35-41","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Polymerase chain reaction for the diagnosis of herpesvirus infections in dermatology"],"prefix":"10.1007","volume":"132","author":[{"given":"Verena","family":"Schremser","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukasz","family":"Antoniewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Tschachler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Geusau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,9]]},"reference":[{"key":"1585_CR1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1093\/hesc\/9780198714682.001.0001","volume-title":"Human virology","author":"J Oxford","year":"2016","unstructured":"Oxford\u00a0J, Kellam\u00a0P, Collier\u00a0L. 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The study was approved by the ethics committee of the Medical University of Vienna (EK-number 2168\/2013).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical standards"}}]}}