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In this study we collected ticks in popular recreational areas in the city of Vienna in the years 2019 and 2020 and analyzed them for the presence of such putative pathogenic microorganisms. By using reverse line blot (RLB) hybridization we detected DNA of <jats:italic>B.\u00a0burgdorferi<\/jats:italic> s.\u202fl., <jats:italic>Rickettsia<\/jats:italic> spp., <jats:italic>Babesia<\/jats:italic> spp., <jats:italic>Candidatus<\/jats:italic> Neoehrlichia mikurensis (CNM) and <jats:italic>Anaplasma phagocytophilum<\/jats:italic>. Moreover, we also screened them for the relapsing fever spirochete <jats:italic>Borrelia miyamotoi<\/jats:italic> employing real-time PCR. The most frequently detected pathogens were <jats:italic>B.\u00a0burgdorferi<\/jats:italic> s.\u202fl. in 28.6% of the ticks in 2019 and 21.3% of the ticks in 2020. The genus <jats:italic>Rickettsia<\/jats:italic> was detected in 13.8% of the ticks from 2019 and only in 4.6% from 2020. <jats:italic>Babesia<\/jats:italic> spp. were detected in 5.7% in 2019 and 4.2% in 2020. Furthermore, we detected CNM in 4.0% (2019) and 5.6% (2020), <jats:italic>A.\u00a0phagocytophilum<\/jats:italic> in 0.5% (2019) and 1.3% (2020) and finally <jats:italic>B.\u00a0miyamotoi<\/jats:italic> in 3.3% (2019) and 1.7% (2020). Collectively, we show that various microorganisms are prevalent in ticks collected in Vienna and identify hotspots for <jats:italic>B.\u00a0miyamotoi,<\/jats:italic> which we have detected for the first time in the city.<\/jats:p>","DOI":"10.1007\/s00508-022-02046-7","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T19:02:39Z","timestamp":1654887759000},"page":"177-184","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Bacteria and protozoa with pathogenic potential in Ixodes ricinus ticks in Viennese recreational areas"],"prefix":"10.1007","volume":"135","author":[{"given":"Anna-Margarita","family":"Sch\u00f6tta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Stelzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerold","family":"Stanek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannes","family":"Stockinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4559-5960","authenticated-orcid":false,"given":"Michiel","family":"Wijnveld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"issue":"1-2","key":"2046_CR1","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/s0176-6724(86)80106-7","volume":"263","author":"A Radda","year":"1986","unstructured":"Radda\u00a0A, Burger\u00a0I, Stanek\u00a0G, Wewalka\u00a0G. 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