{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:34:16Z","timestamp":1760132056252},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,5,19]],"date-time":"2009-05-19T00:00:00Z","timestamp":1242691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Hepato Biliary Pancreat"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In experimental liver transplantation, endogenous protease inhibitors alleviate ischemia\u2013reperfusion (I\/R) injury by inhibiting proteolysis and by direct anti\u2010inflammatory actions. We described the kinetics of endogenous protease inhibitors and explored their anti\u2010inflammatory potential during reperfusion and their effects on graft function in human liver transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We measured circulating levels of protease inhibitors (secretory leukocyte proteinase inhibitor, SLPI; tissue inhibitor of metalloproteinases\u20101, TIMP\u20101) and proteolytic enzymes (elastase; matrix metalloproteinase\u20109, MMP\u20109) with ELISA, and neutrophil and monocyte CD11b and<jats:sc>l<\/jats:sc>\u2010selectin expression with flow cytometry during liver transplantation in ten patients. To assess changes within the graft during reperfusion, blood samples from portal and hepatic veins were obtained simultaneously.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Circulating SLPI and TIMP\u20101 levels decreased during surgery. During initial reperfusion, the transhepatic SLPI gradient was \u221227 (\u221235 to \u221222) ng\/ml,<jats:italic>P<\/jats:italic> = 0.005, and TIMP\u20101 \u2212510 (\u2212636 to \u2212362) ng\/ml,<jats:italic>P<\/jats:italic> = 0.005, indicating graft protease inhibitor uptake. Concomitantly, hepatic phagocyte activation and sequestration as well as elastase and MMP\u20109 release into the circulation occurred. The transhepatic SLPI gradient correlated with postoperative liver enzymes (ALT<jats:italic>R<\/jats:italic> = \u22120.648,<jats:italic>P<\/jats:italic> = 0.043; ALP<jats:italic>R<\/jats:italic> = \u22120.661,<jats:italic>P<\/jats:italic> = 0.038; bilirubin<jats:italic>R<\/jats:italic> = \u22120.821,<jats:italic>P<\/jats:italic> = 0.004; GGT<jats:italic>R<\/jats:italic> = \u22120.648,<jats:italic>P<\/jats:italic> = 0.043).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results suggest a relative shortage of protease inhibitors within the liver during reperfusion, which may contribute to the development of graft injury.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s00534-009-0125-3","type":"journal-article","created":{"date-parts":[[2009,5,18]],"date-time":"2009-05-18T07:38:49Z","timestamp":1242632329000},"page":"158-165","source":"Crossref","is-referenced-by-count":8,"title":["Endogenous protease inhibitor uptake within the graft during reperfusion in human liver transplantation"],"prefix":"10.1002","volume":"17","author":[{"given":"Minna","family":"Ilmakunnas","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine Helsinki University Hospital  P.O. Box 340 Haartmaninkatu 4 00029 HUS Finland"},{"name":"Transplantation and Liver Surgery Clinic, Department of Surgery Helsinki University Hospital  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krister","family":"H\u00f6ckerstedt","sequence":"additional","affiliation":[{"name":"Transplantation and Liver Surgery Clinic, Department of Surgery Helsinki University Hospital  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heikki","family":"M\u00e4kisalo","sequence":"additional","affiliation":[{"name":"Transplantation and Liver Surgery Clinic, Department of Surgery Helsinki University Hospital  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanna","family":"Siitonen","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Helsinki University Hospital  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heikki","family":"Repo","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Haartman Institute University of Helsinki  Helsinki Finland"},{"name":"Department of Medicine Helsinki University Hospital  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eero J.","family":"Pesonen","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine Helsinki University Hospital  P.O. Box 340 Haartmaninkatu 4 00029 HUS Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,5,19]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4800(03)00008-X"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199311000-00005"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200004150-00042"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199402150-00007"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000120384.52033.BC"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510260418"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.61.6.695"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.0714d.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840180624"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70355-0"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119254"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3684-3687.2004"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.11523"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0279fje"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2008.02158.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90312-U"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200005000-00003"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.154.2.8756805"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH03-05-0272"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0750053"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.28.16518"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715769809065797"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2551036"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-4666(02)00485-4"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6346(03)00092-5"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199611000-00023"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v112.pm9024309"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21922"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1999.5661"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-6135.2003.00277.x"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90701-7"}],"container-title":["Journal of Hepato-Biliary-Pancreatic Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1007%2Fs00534-009-0125-3","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s00534-009-0125-3","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1007\/s00534-009-0125-3","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s00534-009-0125-3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T15:29:19Z","timestamp":1694446159000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1007\/s00534-009-0125-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5,19]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1007\/s00534-009-0125-3"],"URL":"https:\/\/doi.org\/10.1007\/s00534-009-0125-3","archive":["Portico"],"relation":{},"ISSN":["1868-6974","1868-6982"],"issn-type":[{"value":"1868-6974","type":"print"},{"value":"1868-6982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5,19]]}}}