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Blood was withdrawn into blood collection pack and shaken at 60\u201380\u00a0rpm for 24\u00a0h in room temperature. Clot formation was evaluated using rotational thromboelastometry (ROTEM\u2122) just after blood withdrawal (control) and 4, 8, 12 and 24\u00a0h after blood withdrawal. We compared with the control value and each value of extrinsically-activated test with tissue factor (EXTEM), intrinsically-activated test using ellagic acid (INTEM) and fibrin-based extrinsically activated test with tissue factor (FIBTEM).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Maximum clot firmness (MCF) of FIBTEM did not change significantly. MCF of EXTEM was significantly decreased time-dependent manner but all MCF of EXTEM were within a normal range. Maximum percent change in MCF of EXTEM was 12.4% [95% confidence interval (CI): 9.0\u201315.8%]. The difference in the maximum clot elasticity (MCE) between EXTEM and FIBTEM (MCE<jats:sub>EXTEM<\/jats:sub>\u2212MCE<jats:sub>FIBTEM<\/jats:sub>) was significantly decrease from 8\u00a0h after blood withdrawal. Maximum percent change in MCE<jats:sub>EXTEM<\/jats:sub>\u2212MCE<jats:sub>FIBTEM<\/jats:sub> was 30.2% (95% CI:17.6\u201342.9%) at 24\u00a0h after blood withdrawal.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Even though the MCE significantly decreased in a time-dependent manner, MCF of FIBTEM and EXTEM was normal up to 24\u00a0h storage. The blood of ANH can use for the purpose of hemostasis at least 8\u00a0h stored at room temperature after blood withdrawal. Future studies are needed to elucidate the clinical impact on the patient after delayed transfusion of ANH blood with regard to patient\u2019s hemostasis.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00540-020-02856-x","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T13:04:16Z","timestamp":1601039056000},"page":"35-42","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Clotting functional stability of withdrawing blood in storage for acute normovolemic hemodilution: a pilot study"],"prefix":"10.1007","volume":"35","author":[{"given":"Hirotaka","family":"Kinoshita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6040-4926","authenticated-orcid":false,"given":"Junichi","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kishiko","family":"Nakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoko","family":"Noguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiki","family":"Takekawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiko","family":"Tamai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Kitayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyoshi","family":"Hirota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,9,25]]},"reference":[{"key":"2856_CR1","doi-asserted-by":"publisher","first-page":"4529","DOI":"10.7314\/APJCP.2013.14.8.4529","volume":"14","author":"JR Guo","year":"2013","unstructured":"Guo JR, Jin XJ, Yu J, Xu F, Zhang YW, Shen HC, Shao Y. 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All authors read and approved the final manuscript and attest to the integrity of the original data and the analysis reported in this manuscript.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study protocol was approved by Hirosaki University's Ethics Committee (2018\u20131040).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}