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The subtribe Calypsoinae illustrates this diversity through repeated shifts in fungal partners and nutritional strategies. Recent phylogenomic analyses place the\n                    <jats:italic>Dactylostalix<\/jats:italic>\n                    \u2013\n                    <jats:italic>Ephippianthus<\/jats:italic>\n                    clade as sister to the\n                    <jats:italic>Corallorhiza<\/jats:italic>\n                    clade, which includes several heterotrophic lineages, raising questions about adult nutrition in\n                    <jats:italic>Ephippianthus<\/jats:italic>\n                    . Here, we combined fungal metabarcoding with \u03b4\u00b9\u00b3C and \u03b4\u00b9\u2075N analyses to investigate the physiological ecology of the two known\n                    <jats:italic>Ephippianthus<\/jats:italic>\n                    species, which are restricted to Japan and the adjacent Russian Far East. We found that\n                    <jats:italic>Ephippianthus schmidtii<\/jats:italic>\n                    was primarily associated with Tulasnellaceae (Cantharellales). Its \u03b4\u00b9\u00b3C and \u03b4\u00b9\u2075N values showed no significant positive enrichment relative to co-occurring autotrophic plants, providing no evidence for substantial fungal nutrient gain based on these isotope markers. By contrast,\n                    <jats:italic>Ephippianthus sawadanus<\/jats:italic>\n                    was mainly associated with saprotrophic Cyphellaceae (Agaricales), representing, to our knowledge, the first documented case in which a member of this family was detected as the dominant mycorrhizal associate of a green orchid. Moreover,\n                    <jats:italic>E. sawadanus<\/jats:italic>\n                    exhibited elevated \u03b4\u00b9\u00b3C and \u03b4\u00b9\u2075N values relative to autotrophic plants, consistent with substantial fungal contributions to its carbon and nitrogen budgets. Together, these findings reveal divergent mycorrhizal strategies and nutritional modes in the East Asian orchid genus\n                    <jats:italic>Ephippianthus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1007\/s00572-026-01285-0","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:52:28Z","timestamp":1782953548000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Divergent mycorrhizal strategies and nutritional modes in the East Asian orchid genus Ephippianthus"],"prefix":"10.1007","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7943-4164","authenticated-orcid":false,"given":"Kenji","family":"Suetsugu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidehito","family":"Okada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"1285_CR1","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"SF Altschul","year":"1997","unstructured":"Altschul SF, Madden TL, Sch\u00e4ffer AA et al (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. 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