{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:09:36Z","timestamp":1762542576103},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T00:00:00Z","timestamp":1471910400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Diabetol"],"published-print":{"date-parts":[[2016,12]]},"DOI":"10.1007\/s00592-016-0896-3","type":"journal-article","created":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T05:11:16Z","timestamp":1471929076000},"page":"935-944","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A stable isotope method for in vivo assessment of human insulin synthesis and secretion"],"prefix":"10.1007","volume":"53","author":[{"given":"Sjaam","family":"Jainandunsing","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joram N. I.","family":"van Miert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trinet","family":"Rietveld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. L.","family":"Darcos Wattimena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric J. G.","family":"Sijbrands","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix W. M.","family":"de Rooij","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,8,23]]},"reference":[{"issue":"Suppl 2","key":"896_CR1","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1016\/S1262-3636(08)73394-9","volume":"34","author":"PJ Guillausseau","year":"2008","unstructured":"Guillausseau PJ, Meas T, Virally M, Laloi-Michelin M, Medeau V, Kevorkian JP (2008) Abnormalities in insulin secretion in type 2 diabetes mellitus. Diabetes Metab 34(Suppl 2):S43\u2013S48","journal-title":"Diabetes Metab"},{"key":"896_CR2","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1172\/JCI29103","volume":"116","author":"M Prentki","year":"2006","unstructured":"Prentki M, Nolan CJ (2006) Islet beta cell failure in type 2 diabetes. J Clin Invest 116:1802\u20131812","journal-title":"J Clin Invest"},{"issue":"Suppl 2","key":"896_CR3","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/j.1463-1326.2007.00774.x","volume":"9","author":"Y Uchizono","year":"2007","unstructured":"Uchizono Y, Alarcon C, Wicksteed BL, Marsh BJ, Rhodes CJ (2007) The balance between proinsulin biosynthesis and insulin secretion: Where can imbalance lead? Diabetes Obes Metab 9(Suppl 2):56\u201366","journal-title":"Diabetes Obes Metab"},{"key":"896_CR4","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1210\/endo-83-3-572","volume":"83","author":"DL Curry","year":"1968","unstructured":"Curry DL, Bennett LL, Grodsky GM (1968) Dynamics of insulin secretion by the perfused rat pancreas. Endocrinology 83:572\u2013584","journal-title":"Endocrinology"},{"key":"896_CR5","doi-asserted-by":"crossref","first-page":"E371","DOI":"10.1152\/ajpendo.00139.2003","volume":"287","author":"A Caumo","year":"2004","unstructured":"Caumo A, Luzi L (2004) First-phase insulin secretion: Does it exist in real life? Considerations on shape and function. Am J Physiol Endocrinol Metab 287:E371\u2013E385","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"896_CR6","doi-asserted-by":"crossref","first-page":"224","DOI":"10.2337\/diab.21.4.224","volume":"21","author":"E Cerasi","year":"1972","unstructured":"Cerasi E, Luft R, Efendic S (1972) Decreased sensitivity of the pancreatic beta cells to glucose in prediabetic and diabetic subjects. A glucose dose-response study. Diabetes 21:224\u2013234","journal-title":"Diabetes"},{"key":"896_CR7","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1007\/s00125-007-0644-x","volume":"50","author":"CC Richardson","year":"2007","unstructured":"Richardson CC, Hussain K, Jones PM et al (2007) Low levels of glucose transporters and K\u00a0+\u00a0ATP channels in human pancreatic beta cells early in development. Diabetologia 50:1000\u20131005","journal-title":"Diabetologia"},{"issue":"Suppl 4","key":"896_CR8","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/j.1463-1326.2009.01109.x","volume":"11","author":"JC Henquin","year":"2009","unstructured":"Henquin JC, Nenquin M, Ravier MA, Szollosi A (2009) Shortcomings of current models of glucose-induced insulin secretion. Diabetes Obes Metab 11(Suppl 4):168\u2013179","journal-title":"Diabetes Obes Metab"},{"key":"896_CR9","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.2337\/diab.36.10.1167","volume":"36","author":"J Calles-Escandon","year":"1987","unstructured":"Calles-Escandon J, Robbins DC (1987) Loss of early phase of insulin release in humans impairs glucose tolerance and blunts thermic effect of glucose. Diabetes 36:1167\u20131172","journal-title":"Diabetes"},{"key":"896_CR10","doi-asserted-by":"crossref","first-page":"983","DOI":"10.2337\/diacare.24.6.983","volume":"24","author":"PA Hollander","year":"2001","unstructured":"Hollander PA, Schwartz SL, Gatlin MR et al (2001) Importance of early insulin secretion: comparison of nateglinide and glyburide in previously diet-treated patients with type 2 diabetes. Diabetes Care 24:983\u2013988","journal-title":"Diabetes Care"},{"key":"896_CR11","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1172\/JCI104130","volume":"39","author":"RS Yalow","year":"1960","unstructured":"Yalow RS, Berson SA (1960) Immunoassay of endogenous plasma insulin in man. J Clin Invest 39:1157\u20131175","journal-title":"J Clin Invest"},{"key":"896_CR12","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1172\/JCI107011","volume":"51","author":"GM Grodsky","year":"1972","unstructured":"Grodsky GM (1972) A threshold distribution hypothesis for packet storage of insulin and its mathematical modeling. J Clin Invest 51:2047\u20132059","journal-title":"J Clin Invest"},{"key":"896_CR13","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1242\/jcs.034355","volume":"122","author":"Z Wang","year":"2009","unstructured":"Wang Z, Thurmond DC (2009) Mechanisms of biphasic insulin-granule exocytosis\u2014roles of the cytoskeleton, small GTPases and SNARE proteins. J Cell Sci 122:893\u2013903","journal-title":"J Cell Sci"},{"key":"896_CR14","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.regpep.2007.09.019","volume":"146","author":"GM Portela-Gomes","year":"2008","unstructured":"Portela-Gomes GM, Grimelius L, Stridsberg M (2008) Prohormone convertases 1\/3, 2, furin and protein 7B2 (Secretogranin V) in endocrine cells of the human pancreas. Regul Pept 146:117\u2013124","journal-title":"Regul Pept"},{"key":"896_CR15","first-page":"R378","volume":"238","author":"MD O\u2019Connor","year":"1980","unstructured":"O\u2019Connor MD, Landahl H, Grodsky GM (1980) Comparison of storage- and signal-limited models of pancreatic insulin secretion. Am J Physiol 238:R378\u2013R389","journal-title":"Am J Physiol"},{"key":"896_CR16","first-page":"72","volume":"15","author":"P Rorsman","year":"2000","unstructured":"Rorsman P, Eliasson L, Renstrom E, Gromada J, Barg S, Gopel S (2000) The cell physiology of biphasic insulin secretion. News Physiol Sci 15:72\u201377","journal-title":"News Physiol Sci"},{"key":"896_CR17","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1042\/bj1020922","volume":"102","author":"SL Howell","year":"1967","unstructured":"Howell SL, Taylor KW (1967) The secretion of newly synthesized insulin in vitro. Biochem J 102:922\u2013927","journal-title":"Biochem J"},{"key":"896_CR18","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1083\/jcb.105.1.145","volume":"105","author":"CJ Rhodes","year":"1987","unstructured":"Rhodes CJ, Halban PA (1987) Newly synthesized proinsulin\/insulin and stored insulin are released from pancreatic B cells predominantly via a regulated, rather than a constitutive, pathway. J Cell Biol 105:145\u2013153","journal-title":"J Cell Biol"},{"key":"896_CR19","first-page":"E797","volume":"259","author":"PE Ballmer","year":"1990","unstructured":"Ballmer PE, McNurlan MA, Milne E et al (1990) Measurement of albumin synthesis in humans: a new approach employing stable isotopes. Am J Physiol 259:E797\u2013E803","journal-title":"Am J Physiol"},{"key":"896_CR20","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1016\/j.metabol.2014.09.009","volume":"63","author":"D Tuvdendorj","year":"2014","unstructured":"Tuvdendorj D, Chinkes DL, Bahadorani J et al (2014) Comparison of bolus injection and constant infusion methods for measuring muscle protein fractional synthesis rate in humans. Metabolism 63:1562\u20131567","journal-title":"Metabolism"},{"key":"896_CR21","doi-asserted-by":"crossref","first-page":"E753","DOI":"10.1152\/ajpendo.00053.2002","volume":"283","author":"XJ Zhang","year":"2002","unstructured":"Zhang XJ, Chinkes DL, Wolfe RR (2002) Measurement of muscle protein fractional synthesis and breakdown rates from a pulse tracer injection. Am J Physiol Endocrinol Metab 283:E753\u2013E764","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"896_CR22","doi-asserted-by":"crossref","first-page":"5892","DOI":"10.1073\/pnas.88.13.5892","volume":"88","author":"PW Watt","year":"1991","unstructured":"Watt PW, Lindsay Y, Scrimgeour CM et al (1991) Isolation of aminoacyl-tRNA and its labeling with stable-isotope tracers: use in studies of human tissue protein synthesis. Proc Nat Acad Sci USA 88:5892\u20135896","journal-title":"Proc Nat Acad Sci USA"},{"key":"896_CR23","first-page":"E73","volume":"275","author":"K Smith","year":"1998","unstructured":"Smith K, Reynolds N, Downie S, Patel A, Rennie MJ (1998) Effects of flooding amino acids on incorporation of labeled amino acids into human muscle protein. Am J Physiol 275:E73\u2013E78","journal-title":"Am J Physiol"},{"key":"896_CR24","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/BF01062525","volume":"20","author":"RD Purves","year":"1992","unstructured":"Purves RD (1992) Optimum numerical integration methods for estimation of area-under-the-curve (AUC) and area-under-the-moment-curve (AUMC). J Pharmacokinet Biopharm 20:211\u2013226","journal-title":"J Pharmacokinet Biopharm"},{"key":"896_CR25","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1126\/science.6181562","volume":"218","author":"G Gold","year":"1982","unstructured":"Gold G, Gishizky ML, Grodsky GM (1982) Evidence that glucose \u201cmarks\u201d beta cells resulting in preferential release of newly synthesized insulin. Science 218:56\u201358","journal-title":"Science"},{"key":"896_CR26","doi-asserted-by":"crossref","first-page":"e47921","DOI":"10.1371\/journal.pone.0047921","volume":"7","author":"N Hou","year":"2012","unstructured":"Hou N, Mogami H, Kubota-Murata C, Sun M, Takeuchi T, Torii S (2012) Preferential release of newly synthesized insulin assessed by a multi-label reporter system using pancreatic beta-cell line MIN6. PLoS One 7:e47921","journal-title":"PLoS One"},{"key":"896_CR27","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1172\/JCI106944","volume":"51","author":"H Sando","year":"1972","unstructured":"Sando H, Borg J, Steiner DF (1972) Studies on the secretion of newly synthesized proinsulin and insulin from isolated rat islets of Langerhans. J Clin Invest 51:1476\u20131485","journal-title":"J Clin Invest"},{"key":"896_CR28","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1038\/oby.2010.70","volume":"18","author":"JE Galgani","year":"2010","unstructured":"Galgani JE, de Jonge L, Rood JC, Smith SR, Young AA, Ravussin E (2010) Urinary C-peptide excretion: a novel alternate measure of insulin sensitivity in physiological conditions. Obesity 18:1852\u20131857","journal-title":"Obesity"},{"key":"896_CR29","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0005-2744(75)90280-6","volume":"377","author":"RT Eakin","year":"1975","unstructured":"Eakin RT (1975) Kinetic properties of an enzyme highly enriched in carbon-13. Biochim Biophys Acta 377:9\u201314","journal-title":"Biochim Biophys Acta"},{"key":"896_CR30","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/0026-0495(91)90043-V","volume":"40","author":"F Carraro","year":"1991","unstructured":"Carraro F, Rosenblatt J, Wolfe RR (1991) Isotopic determination of fibronectin synthesis in humans. Metabolism 40:553\u2013561","journal-title":"Metabolism"},{"key":"896_CR31","first-page":"E548","volume":"255","author":"RE Shangraw","year":"1988","unstructured":"Shangraw RE, Stuart CA, Prince MJ, Peters EJ, Wolfe RR (1988) Insulin responsiveness of protein metabolism in vivo following bedrest in humans. Am J Physiol 255:E548\u2013E558","journal-title":"Am J Physiol"},{"key":"896_CR32","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/0026-0495(93)90234-F","volume":"42","author":"WM Bennet","year":"1993","unstructured":"Bennet WM, O\u2019Keefe SJ, Haymond MW (1993) Comparison of precursor pools with leucine, alpha-ketoisocaproate, and phenylalanine tracers used to measure splanchnic protein synthesis in man. Metabolism 42:691\u2013695","journal-title":"Metabolism"},{"key":"896_CR33","doi-asserted-by":"crossref","first-page":"12513","DOI":"10.1074\/jbc.272.19.12513","volume":"272","author":"AD Kippen","year":"1997","unstructured":"Kippen AD, Cerini F, Vadas L et al (1997) Development of an isotope dilution assay for precise determination of insulin, C-peptide, and proinsulin levels in non-diabetic and type II diabetic individuals with comparison to immunoassay. J Biol Chem 272:12513\u201312522","journal-title":"J Biol Chem"},{"key":"896_CR34","doi-asserted-by":"crossref","first-page":"E365","DOI":"10.1152\/ajpendo.2001.281.2.E365","volume":"281","author":"E Blomstrand","year":"2001","unstructured":"Blomstrand E, Saltin B (2001) BCAA intake affects protein metabolism in muscle after but not during exercise in humans. Am J Physiol Endocrinol Metab 281:E365\u2013E374","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"896_CR35","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1113\/jphysiol.1986.sp016314","volume":"381","author":"JC Henquin","year":"1986","unstructured":"Henquin JC, Meissner HP (1986) Cyclic adenosine monophosphate differently affects the response of mouse pancreatic beta-cells to various amino acids. J Physiol 381:77\u201393","journal-title":"J Physiol"},{"key":"896_CR36","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1111\/j.1753-4887.2010.00282.x","volume":"68","author":"J Yang","year":"2010","unstructured":"Yang J, Chi Y, Burkhardt BR, Guan Y, Wolf BA (2010) Leucine metabolism in regulation of insulin secretion from pancreatic beta cells. Nutr Rev 68:270\u2013279","journal-title":"Nutr Rev"},{"key":"896_CR37","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1002\/iub.375","volume":"62","author":"MC Gannon","year":"2010","unstructured":"Gannon MC, Nuttall FQ (2010) Amino acid ingestion and glucose metabolism\u2014a review. IUBMB Life 62:660\u2013668","journal-title":"IUBMB Life"},{"key":"896_CR38","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.ab.2011.02.009","volume":"414","author":"M Goebel-Stengel","year":"2011","unstructured":"Goebel-Stengel M, Stengel A, Tache Y, Reeve JR Jr (2011) The importance of using the optimal plasticware and glassware in studies involving peptides. Anal Biochem 414:38\u201346","journal-title":"Anal Biochem"},{"key":"896_CR39","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s00216-007-1638-8","volume":"390","author":"S Sturup","year":"2008","unstructured":"Sturup S, Hansen HR, Gammelgaard B (2008) Application of enriched stable isotopes as tracers in biological systems: a critical review. Anal Bioanal Chem 390:541\u2013554","journal-title":"Anal Bioanal Chem"}],"container-title":["Acta Diabetologica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00592-016-0896-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00592-016-0896-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00592-016-0896-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,12]],"date-time":"2019-09-12T21:30:29Z","timestamp":1568323829000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00592-016-0896-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,23]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["896"],"URL":"https:\/\/doi.org\/10.1007\/s00592-016-0896-3","relation":{},"ISSN":["0940-5429","1432-5233"],"issn-type":[{"value":"0940-5429","type":"print"},{"value":"1432-5233","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,23]]}}}