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To construct the nanoprobe, newly synthesized glutathione-capped ZnS\/Ag<jats:sub>2<\/jats:sub>Se quantum dots (QDs) were first conjugated to graphene oxide (GO) to form a QD-GO nanocomposite. The binding interaction resulted in a fluorescence turn-ON. Thereafter, cetyltrimethylammonium bromide (CTAB)-gold nanoparticles (AuNPs) were directly adsorbed on the QD-GO nanocomposite to form a novel QD-GO-CTAB-AuNP nanohybrid assembly that resulted in a fluorescence turn-OFF. Streptavidin (strep) was then adsorbed on the QDs-GO-CTAB-AuNP nanohybrid assembly which allowed binding to a biotinylated MNS 4.1 anticocaine DNA aptamer (B) receptor. The addition of cocaine into the strep-B-QDs-GO-CTAB-AuNP aptamer nanoprobe system aided affinity to the aptamer receptor and in turn turned on the fluorescence of the nanoprobe in a concentration-dependent manner. Under optimum experimental conditions, we found the strep-B-QD-GO-CTAB-AuNP to be far superior in its\u00a0sensitivity to cocaine than the tested strep-B-QDs (no GO and CTAB-AuNPs), strep-B-QD-CTAB-AuNP (no GO) and strep-B-QD-GO (no CTAB-AuNP). In addition, the investigation of localized surface plasmon resonance (LSPR) amplified signal from tested plasmonic NPs shows that CTAB-AuNPs was far superior in amplifying the fluorescence signal of the nanoprobe. A detection limit of 4.6\u00a0nM (1.56\u00a0ng.mL<jats:sup>\u22121<\/jats:sup>), rapid response time (~2\u00a0min) and excellent selectivity against other drugs, substances and cocaine metabolites was achieved. The strep-B-QD-GO-CTAB-AuNP aptamer-based fluorescent nanoprobe was successfully applied for the determination of cocaine in seized adulterated cocaine samples.<\/jats:p>","DOI":"10.1007\/s00604-019-4101-6","type":"journal-article","created":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T03:02:30Z","timestamp":1578452550000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Aptamer-based cocaine assay\u00a0using a nanohybrid composed of ZnS\/Ag2Se quantum dots, graphene oxide and gold nanoparticles as a fluorescent probe"],"prefix":"10.1007","volume":"187","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8087-460X","authenticated-orcid":false,"given":"Oluwasesan","family":"Adegoke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda A.","family":"Pereira-Barros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6621-7649","authenticated-orcid":false,"given":"Svetlana","family":"Zolotovskaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6934-5320","authenticated-orcid":false,"given":"Amin","family":"Abdolvand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9338-0887","authenticated-orcid":false,"given":"Niamh Nic","family":"Daeid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,8]]},"reference":[{"key":"4101_CR1","doi-asserted-by":"publisher","first-page":"11100","DOI":"10.1021\/ac503360n","volume":"86","author":"D Roncancio","year":"2014","unstructured":"Roncancio D, Yu H, Xu X, Wu S, Liu R, Debord J, Lou X, Xiao Y (2014) A label-free aptamer-fluorophore assembly for rapid and specific detection of cocaine in biofluids. 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