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Initially, platinum nanozymes were loaded into the dendrimers through an in situ synthetic method. Then, GOD and monoclonal anti-CRP antibody with a high molar ratio were covalently conjugated onto carboxylated dendrimers via typical carbodiimide coupling. The immunoreaction was carried out with a competitive mode in a\u00a0CRP-coated microplate. Along with formation of immunocomplex, the added glucose was oxidized into gluconic acid and hydrogen peroxide by GOD, and the latter was further decomposed by platinum nanozyme, thus accelerating chemical reaction in the positive direction. The produced gluconic acid changed the pH of detection solution, which was determined using\u00a0a handheld pH meter. Under optimum conditions, the pH meter\u2013based immunoassay gave a good signal toward target CRP from 0.01 to 100\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>. The limit of detection was 5.9\u00a0pg\u00a0mL<jats:sup>\u22121<\/jats:sup>. An intermediate precision \u2264\u200911.2% was acquired with batch-to-batch identification. No nonspecific adsorption was observed during a series of procedures to detect target CRP, and the cross-reaction against other biomarkers was very low. Importantly, our system gave well-matched results for analysis of human serum samples relative to a\u00a0referenced ELISA kit.<\/jats:p><jats:p>Graphical abstract<\/jats:p>","DOI":"10.1007\/s00604-020-04687-9","type":"journal-article","created":{"date-parts":[[2021,1,3]],"date-time":"2021-01-03T05:02:22Z","timestamp":1609650142000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Handheld pH meter\u2013assisted immunoassay for C-reactive protein using glucose oxidase\u2013conjugated dendrimer loaded with platinum nanozymes"],"prefix":"10.1007","volume":"188","author":[{"given":"Bin","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilin","family":"Ge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Lyu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meijuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiongfei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuping","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang Fai","family":"Kwok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,1,3]]},"reference":[{"key":"4687_CR1","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.dsx.2020.02.004","volume":"14","author":"A Azam","year":"2020","unstructured":"Azam A, Fatemeh R, Fatemeh A (2020) Role of C-reactive protein CRP. or high-sensitivity CRP in predicting gestational diabetes mellitus: systematic review. 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