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There is a need for testing technologies to detect the presence of pesticide residues in water sources, especially in developing countries where access to standard laboratory methods is cost prohibitive. Herein, we outline the development of a facile electrochemical sensor for amperometric determination of organophosphorus pesticides in environmental water samples. A three-electrode system was fabricated via UV laser-inscribing on a polyimide film. The working electrode was functionalized with copper nanoparticles with affinity toward organophosphate compounds. The sensor showed a limit of detection (LOD) of 3.42\u2009\u00b1\u20091.69\u00a0\u00b5M for glyphosate, 7.28\u2009\u00b1\u20091.20\u00a0\u00b5M for glufosinate, and 17.78\u2009\u00b1\u20097.68\u00a0\u00b5M for aminomethylphosphonic acid (AMPA). Sensitivity was highest for glyphosate (145.52\u2009\u00b1\u200936.73 nA\u22c5\u00b5M<jats:sup>\u22121<\/jats:sup>\u22c5cm<jats:sup>\u22122<\/jats:sup>) followed by glufosinate (56.98\u2009\u00b1\u200910.87 nA\u22c5\u00b5M<jats:sup>\u22121<\/jats:sup>\u22c5cm<jats:sup>\u22122<\/jats:sup>), and AMPA (30.92\u2009\u00b1\u20098.51 nA\u22c5\u00b5M<jats:sup>\u22121<\/jats:sup>\u22c5cm<jats:sup>\u22122<\/jats:sup>). The response of the sensor is not significantly affected by the presence of several ions and organic molecules commonly present in natural water samples. The developed sensor shows promising potential for facilitating environmental monitoring of organophosphorus pesticide residues, which is a current need in several parts of the world.<\/jats:p><jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s00604-022-05355-w","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T18:04:21Z","timestamp":1655143461000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Development of a nanocopper-decorated laser-scribed sensor for organophosphorus pesticide monitoring in aqueous samples"],"prefix":"10.1007","volume":"189","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2149-1964","authenticated-orcid":false,"given":"David","family":"Bahamon-Pinzon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7008-0363","authenticated-orcid":false,"given":"Geisianny","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3317-7147","authenticated-orcid":false,"given":"Sherine","family":"Obare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9858-0960","authenticated-orcid":false,"given":"Diana","family":"Vanegas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,6,13]]},"reference":[{"issue":"1","key":"5355_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12302-016-0070-0","volume":"28","author":"CM Benbrook","year":"2016","unstructured":"Benbrook CM (2016) Trends in glyphosate herbicide use in the United States and globally. 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