{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:16:23Z","timestamp":1782792983117,"version":"3.54.5"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T00:00:00Z","timestamp":1687478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T00:00:00Z","timestamp":1687478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microchim Acta"],"published-print":{"date-parts":[[2023,7]]},"DOI":"10.1007\/s00604-023-05848-2","type":"journal-article","created":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T20:19:31Z","timestamp":1687551571000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Electrochemical biosensor strategy combining DNA entropy-driven technology to activate CRISPR-Cas13a activity and triple-stranded nucleic acids to detect SARS-CoV-2 RdRp gene"],"prefix":"10.1007","volume":"190","author":[{"given":"Chenyi","family":"Zhuo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zichun","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuying","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanxun","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianli","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7021-5395","authenticated-orcid":false,"given":"Kai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlei","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianjiu","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,6,23]]},"reference":[{"issue":"2","key":"5848_CR1","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1007\/s41664-021-00186-9","volume":"5","author":"JJ Zhu","year":"2021","unstructured":"Zhu JJ (2021) Special topic: biomedical application of DNA-assembled nanostructure. J Anal Test 5(2):93\u201394","journal-title":"J Anal Test"},{"issue":"2","key":"5848_CR2","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1021\/acs.analchem.1c03051","volume":"94","author":"J Pan","year":"2022","unstructured":"Pan J, He Y, Liu Z, Chen J (2022) Tetrahedron-based constitutional dynamic network for COVID-19 or other coronaviruses diagnostics and its logic gate applications. Anal Chem 94(2):714\u2013722","journal-title":"Anal Chem"},{"key":"5848_CR3","doi-asserted-by":"publisher","first-page":"132472","DOI":"10.1016\/j.cej.2021.132472","volume":"429","author":"K Zhang","year":"2022","unstructured":"Zhang K, Fan Z, Ding Y, Xie M (2022) A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR\/Cas12a trans-activity. Chem Eng J 429:132472","journal-title":"Chem Eng J"},{"issue":"34","key":"5848_CR4","doi-asserted-by":"publisher","first-page":"11809","DOI":"10.1021\/acs.analchem.1c02361","volume":"93","author":"L Yu","year":"2021","unstructured":"Yu L, Zhu L, Yan M, Feng S, Huang J, Yang X (2021) Electrochemiluminescence biosensor based on entropy-driven amplification and a tetrahedral DNA nanostructure for miRNA-133a detection. Anal Chem 93(34):11809\u201311815","journal-title":"Anal Chem"},{"key":"5848_CR5","doi-asserted-by":"publisher","first-page":"113015","DOI":"10.1016\/j.bios.2021.113015","volume":"178","author":"Z Fan","year":"2021","unstructured":"Fan Z, Yao B, Ding Y, Zhao J, Xie M, Zhang K (2021) Entropy-driven amplified electrochemiluminescence biosensor for RdRp gene of SARS-CoV-2 detection with self-assembled DNA tetrahedron scaffolds. Biosens Bioelectron 178:113015","journal-title":"Biosens Bioelectron"},{"key":"5848_CR6","doi-asserted-by":"publisher","first-page":"131686","DOI":"10.1016\/j.cej.2021.131686","volume":"427","author":"Z Fan","year":"2022","unstructured":"Fan Z, Yao B, Ding Y, Xu D, Zhao J, Zhang K (2022) Rational engineering the DNA tetrahedrons of dual wavelength ratiometric electrochemiluminescence biosensor for high efficient detection of SARS-CoV-2 RdRp gene by using entropy-driven and bipedal DNA walker amplification strategy. Chem Eng J 427:131686","journal-title":"Chem Eng J"},{"issue":"23","key":"5848_CR7","doi-asserted-by":"publisher","first-page":"5959","DOI":"10.1039\/C9SC01912J","volume":"10","author":"Y Zhang","year":"2019","unstructured":"Zhang Y, Deng Y, Wang C, Li L, Xu L, Yu Y et al (2019) Probing and regulating the activity of cellular enzymes by using DNA tetrahedron nanostructures. Chem Sci 10(23):5959\u20135966","journal-title":"Chem Sci"},{"issue":"5","key":"5848_CR8","doi-asserted-by":"publisher","first-page":"9101","DOI":"10.1021\/acsnano.1c02296","volume":"15","author":"CM Green","year":"2021","unstructured":"Green CM, Hastman DA, Mathur D, Susumu K, Oh E, Medintz IL et al (2021) Direct and efficient conjugation of quantum dots to DNA nanostructures with peptide-PNA. ACS Nano 15(5):9101\u20139110","journal-title":"ACS Nano"},{"issue":"22","key":"5848_CR9","doi-asserted-by":"publisher","first-page":"7602","DOI":"10.1039\/D1SC00587A","volume":"12","author":"D-X Wang","year":"2021","unstructured":"Wang D-X, Wang J, Wang Y-X, Du Y-C, Huang Y, Tang A-N et al (2021) DNA nanostructure-based nucleic acid probes: construction and biological applications. Chem Sci 12(22):7602\u20137622","journal-title":"Chem Sci"},{"issue":"42","key":"5848_CR10","doi-asserted-by":"publisher","first-page":"9758","DOI":"10.1039\/C9SC02281C","volume":"10","author":"J Wang","year":"2019","unstructured":"Wang J, Wang D-X, Ma J-Y, Wang Y-X, Kong D-M (2019) Three-dimensional DNA nanostructures to improve the hyperbranched hybridization chain reaction. Chem Sci 10(42):9758\u20139767","journal-title":"Chem Sci"},{"issue":"11","key":"5848_CR11","doi-asserted-by":"publisher","first-page":"7353","DOI":"10.1021\/acs.analchem.9b01133","volume":"91","author":"J Lu","year":"2019","unstructured":"Lu J, Wang J, Hu X, Gyimah E, Yakubu S, Wang K et al (2019) Electrochemical biosensor based on tetrahedral DNA nanostructures and G-quadruplex\u2013hemin conformation for the ultrasensitive detection of microRNA-21 in serum. Anal Chem 91(11):7353\u20137359","journal-title":"Anal Chem"},{"issue":"20","key":"5848_CR12","doi-asserted-by":"publisher","first-page":"17944","DOI":"10.1021\/am5047735","volume":"6","author":"Z Li","year":"2014","unstructured":"Li Z, Zhao B, Wang D, Wen Y, Liu G, Dong H et al (2014) DNA nanostructure-based universal microarray platform for high-efficiency multiplex bioanalysis in biofluids. ACS Appl Mater Inter 6(20):17944\u201317953","journal-title":"ACS Appl Mater Inter"},{"issue":"44","key":"5848_CR13","doi-asserted-by":"publisher","first-page":"17493","DOI":"10.1021\/jacs.9b09292","volume":"141","author":"L Zhao","year":"2019","unstructured":"Zhao L, Qi X, Yan X, Huang Y, Liang X, Zhang L et al (2019) Engineering aptamer with enhanced affinity by triple helix-based terminal fixation. J Am Chem Soc 141(44):17493\u201317497","journal-title":"J Am Chem Soc"},{"issue":"15","key":"5848_CR14","doi-asserted-by":"publisher","first-page":"5321","DOI":"10.1021\/jacs.6b11470","volume":"139","author":"F Iacovelli","year":"2017","unstructured":"Iacovelli F, Idili A, Benincasa A, Mariottini D, Ottaviani A, Falconi M et al (2017) Simulative and experimental characterization of a pH-dependent clamp-like DNA triple-helix nanoswitch. J Am Chem Soc 139(15):5321\u20135329","journal-title":"J Am Chem Soc"},{"issue":"48","key":"5848_CR15","doi-asserted-by":"publisher","first-page":"15210","DOI":"10.1002\/anie.201701868","volume":"56","author":"Y Hu","year":"2017","unstructured":"Hu Y, Cecconello A, Idili A, Ricci F, Willner I (2017) Triplex DNA nanostructures: from basic properties to applications. Angew Chem Int Ed 56(48):15210\u201315233","journal-title":"Angew Chem Int Ed"},{"issue":"7","key":"5848_CR16","doi-asserted-by":"publisher","first-page":"4184","DOI":"10.1039\/C6SC00694A","volume":"7","author":"Z Zhang","year":"2016","unstructured":"Zhang Z, Wang Y, Zhang N, Zhang S (2016) Self-assembly of nucleic acid molecular aggregates catalyzed by a triple-helix probe for miRNA detection and single cell imaging. Chem Sci 7(7):4184\u20134189","journal-title":"Chem Sci"},{"issue":"16","key":"5848_CR17","doi-asserted-by":"publisher","first-page":"5494","DOI":"10.1002\/chem.201505030","volume":"22","author":"Y Yamagata","year":"2016","unstructured":"Yamagata Y, Emura T, Hidaka K, Sugiyama H, Endo M (2016) Triple helix formation in a topologically controlled DNA nanosystem. Chem Eur J 22(16):5494\u20138","journal-title":"Chem Eur J"},{"key":"5848_CR18","doi-asserted-by":"publisher","first-page":"113994","DOI":"10.1016\/j.bios.2022.113994","volume":"202","author":"F Hu","year":"2022","unstructured":"Hu F, Liu Y, Zhao S, Zhang Z, Li X, Peng N et al (2022) A one-pot CRISPR\/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics. Biosens Bioelectron 202:113994","journal-title":"Biosens Bioelectron"},{"issue":"4","key":"5848_CR19","doi-asserted-by":"publisher","first-page":"2038","DOI":"10.1021\/acs.analchem.0c03708","volume":"93","author":"T Zhou","year":"2021","unstructured":"Zhou T, Huang M, Lin J, Huang R, Xing D (2021) High-fidelity CRISPR\/Cas13a trans-cleavage-triggered rolling circle amplified DNAzyme for visual profiling of microRNA. Anal Chem 93(4):2038\u20132044","journal-title":"Anal Chem"},{"issue":"9","key":"5848_CR20","doi-asserted-by":"publisher","first-page":"3987","DOI":"10.1021\/acs.analchem.1c05124","volume":"94","author":"X Liu","year":"2022","unstructured":"Liu X, Kang X, Lei C, Ren W, Liu C (2022) Programming the trans-cleavage activity of CRISPR-Cas13a by single-strand DNA blocker and its biosensing application. Anal Chem 94(9):3987\u20133996","journal-title":"Anal Chem"},{"issue":"1","key":"5848_CR21","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1039\/D1EN00645B","volume":"9","author":"K Zhang","year":"2022","unstructured":"Zhang K, Fan Z, Ding Y, Zhu S, Xie M, Hao N (2022) Exploring the entropy-driven amplification reaction and trans-cleavage activity of CRISPR-Cas12a for the development of an electrochemiluminescence biosensor for the detection of the SARS-CoV-2 RdRp gene in real samples and environmental surveillance. Environ Sci Nano 9(1):162\u2013172","journal-title":"Environ Sci Nano"},{"issue":"49","key":"5848_CR22","doi-asserted-by":"publisher","first-page":"16528","DOI":"10.1021\/acs.analchem.1c03705","volume":"93","author":"I Lee","year":"2021","unstructured":"Lee I, Kwon S-J, Sorci M, Heeger PS, Dordick JS (2021) Highly sensitive immuno-CRISPR assay for CXCL9 detection. Anal Chem 93(49):16528\u201316534","journal-title":"Anal Chem"},{"issue":"1","key":"5848_CR23","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1021\/acs.analchem.9b04403","volume":"92","author":"Q Chen","year":"2020","unstructured":"Chen Q, Tian T, Xiong E, Wang P, Zhou X (2020) CRISPR\/Cas13a signal amplification linked immunosorbent assay for femtomolar protein detection. Anal Chem 92(1):573\u2013577","journal-title":"Anal Chem"},{"issue":"20","key":"5848_CR24","doi-asserted-by":"publisher","first-page":"7456","DOI":"10.1021\/acs.analchem.1c00525","volume":"93","author":"A Ramachandran","year":"2021","unstructured":"Ramachandran A, Santiago JG (2021) CRISPR enzyme kinetics for molecular diagnostics. Anal Chem 93(20):7456\u20137464","journal-title":"Anal Chem"},{"issue":"57","key":"5848_CR25","doi-asserted-by":"publisher","first-page":"7051","DOI":"10.1039\/D1CC02578C","volume":"57","author":"J Zhao","year":"2021","unstructured":"Zhao J, Tan Z, Wang L, Lei C, Nie Z (2021) A ligation-driven CRISPR\u2013Cas biosensing platform for non-nucleic acid target detections. Chem Commun 57(57):7051\u20137054","journal-title":"Chem Commun"},{"key":"5848_CR26","doi-asserted-by":"crossref","unstructured":"Liang M, Li Z, Wang W, Liu J, Liu L, Zhu G, et al (2019) A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules. Nat Commun 10","DOI":"10.1038\/s41467-019-11648-1"},{"issue":"38","key":"5848_CR27","doi-asserted-by":"publisher","first-page":"12733","DOI":"10.1039\/D1SC02197D","volume":"12","author":"A Suea-Ngam","year":"2021","unstructured":"Suea-Ngam A, Howes PD, deMello AJ (2021) An amplification-free ultra-sensitive electrochemical CRISPR\/Cas biosensor for drug-resistant bacteria detection. Chem Sci 12(38):12733\u201312743","journal-title":"Chem Sci"},{"issue":"6336","key":"5848_CR28","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1126\/science.aam9321","volume":"356","author":"JS Gootenberg","year":"2017","unstructured":"Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J et al (2017) Nucleic acid detection with CRISPR-Cas13a\/C2c2. Science 356(6336):438\u2013442","journal-title":"Science"},{"key":"5848_CR29","doi-asserted-by":"publisher","first-page":"129121","DOI":"10.1016\/j.snb.2020.129121","volume":"329","author":"K Zhang","year":"2021","unstructured":"Zhang K, Zhou L, Zhang T, Fan Z, Xie M, Ding Y et al (2021) Peptide based biosensing of protein functional control indicates novel mechanism of cancerous development under oxidative stress. Sens Actuators B Chem 329:129121","journal-title":"Sens Actuators B Chem"},{"issue":"82","key":"5848_CR30","doi-asserted-by":"publisher","first-page":"10771","DOI":"10.1039\/D1CC03686F","volume":"57","author":"K Zhang","year":"2021","unstructured":"Zhang K, Li J, Fan Z, Li H, Xu J-J (2021) \u201cCovalent biosensing\u201d enables a one-step, reagent-less, low-cost and highly robust assay of SARS-CoV-2. Chem Commun 57(82):10771\u201310774","journal-title":"Chem Commun"},{"key":"5848_CR31","doi-asserted-by":"publisher","first-page":"106837","DOI":"10.1016\/j.microc.2021.106837","volume":"171","author":"D Xu","year":"2021","unstructured":"Xu D, Xu X, Fan Z, Zou M, Qin X, Ding Y et al (2021) Hypersensitive detection of transcription factors by multiple amplification strategy based on molecular beacon. Microchem J 171:106837","journal-title":"Microchem J"},{"key":"5848_CR32","doi-asserted-by":"publisher","first-page":"122868","DOI":"10.1016\/j.talanta.2021.122868","volume":"236","author":"K Zhang","year":"2022","unstructured":"Zhang K, Fan Z, Huang Y, Ding Y, Xie M (2022) A strategy combining 3D-DNA Walker and CRISPR-Cas12a trans-cleavage activity applied to MXene based electrochemiluminescent sensor for SARS-CoV-2 RdRp gene detection. Talanta 236:122868","journal-title":"Talanta"},{"issue":"11","key":"5848_CR33","doi-asserted-by":"publisher","first-page":"4803","DOI":"10.1016\/j.cclet.2022.01.010","volume":"33","author":"X-L Tao","year":"2022","unstructured":"Tao X-L, Pan M-C, Yang X, Yuan R, Zhuo Y (2022) CDs assembled metal-organic framework: exogenous coreactant-free biosensing platform with pore confinement-enhanced electrochemiluminescence. Chin Chem Lett 33(11):4803\u20134807","journal-title":"Chin Chem Lett"},{"issue":"2","key":"5848_CR34","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.cclet.2021.07.017","volume":"33","author":"H Zhao","year":"2022","unstructured":"Zhao H, Su E, Huang L, Zai Y, Liu Y, Chen Z et al (2022) Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin I in whole blood samples. Chin Chem Lett 33(2):743\u2013746","journal-title":"Chin Chem Lett"},{"key":"5848_CR35","doi-asserted-by":"publisher","first-page":"113579","DOI":"10.1016\/j.bios.2021.113579","volume":"194","author":"K Zhang","year":"2021","unstructured":"Zhang K, Fan Z, Ding Y, Li J, Li H (2021) Thiol-sensitive probe enables dynamic electrochemical assembly of serum protein for detecting SARS-Cov-2 marker protease in clinical samples. Biosens Bioelectron 194:113579","journal-title":"Biosens Bioelectron"},{"issue":"65","key":"5848_CR36","doi-asserted-by":"publisher","first-page":"8015","DOI":"10.1039\/D1CC03071J","volume":"57","author":"Z Fan","year":"2021","unstructured":"Fan Z, Ding Y, Yao B, Wang J, Zhang K (2021) Electrochemiluminescence platform for transcription factor diagnosis by using CRISPR\u2013Cas12a trans-cleavage activity. Chem Commun 57(65):8015\u20138018","journal-title":"Chem Commun"},{"key":"5848_CR37","doi-asserted-by":"publisher","first-page":"132966","DOI":"10.1016\/j.cej.2021.132966","volume":"430","author":"N Kumar","year":"2022","unstructured":"Kumar N, Shetti NP, Jagannath S, Aminabhavi TM (2022) Electrochemical sensors for the detection of SARS-CoV-2 virus. Chem Eng J 430:132966","journal-title":"Chem Eng J"},{"key":"5848_CR38","doi-asserted-by":"crossref","unstructured":"Fan Z, Xie M, Pan J, Zhang K (2022) SARS-CoV-2 monitoring by automated target-driven molecular machine based engineering. Environ Chem Lett 20 (4):2227\u20132233","DOI":"10.1007\/s10311-022-01434-9"},{"key":"5848_CR39","doi-asserted-by":"publisher","first-page":"114293","DOI":"10.1016\/j.bios.2022.114293","volume":"210","author":"D Das","year":"2022","unstructured":"Das D, Lin C-W, Kwon JS, Chuang HS (2022) Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA. Biosens Bioelectron 210:114293","journal-title":"Biosens Bioelectron"},{"key":"5848_CR40","doi-asserted-by":"publisher","first-page":"128899","DOI":"10.1016\/j.snb.2020.128899","volume":"327","author":"H Zhao","year":"2021","unstructured":"Zhao H, Liu F, Xie W, Zhou T, OuYang J, Jin L et al (2021) Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone. Sens Actuators B Chem 327:128899","journal-title":"Sens Actuators B Chem"},{"issue":"13","key":"5848_CR41","doi-asserted-by":"publisher","first-page":"1903661","DOI":"10.1002\/advs.201903661","volume":"7","author":"T Zhou","year":"2020","unstructured":"Zhou T, Huang R, Huang M, Shen J, Shan Y, Xing D (2020) CRISPR\/Cas13a powered portable electrochemiluminescence chip for ultrasensitive and specific MiRNA detection. Adv Sci 7(13):1903661","journal-title":"Adv Sci"}],"container-title":["Microchimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-023-05848-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00604-023-05848-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-023-05848-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T16:49:56Z","timestamp":1688489396000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00604-023-05848-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,23]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["5848"],"URL":"https:\/\/doi.org\/10.1007\/s00604-023-05848-2","relation":{},"ISSN":["0026-3672","1436-5073"],"issn-type":[{"value":"0026-3672","type":"print"},{"value":"1436-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,23]]},"assertion":[{"value":"31 January 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 May 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 June 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"272"}}