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Despite there is not an ideal-specific biomarker, the simultaneous detection of biomarkers with different behavior during an infection such as procalcitonin (PCT) as high specificity biomarker with one of the earliest biomarkers in sepsis as interleukin-6 (IL-6) increases diagnostic performance. This is not only due to their high positive predictive value but also, since\u00a0it can also help the clinician to rule out infection and thus avoid the use of antibiotics, due to their high negative predictive value. To this end, we explore a cutting-edge micromotor (MM)-based OFF\u2013ON dual aptassay for simultaneous determination of both biomarkers in 15\u00a0min using just 2 \u03bcL of sample from low-birth-weight neonates with gestational age less than 32\u00a0weeks and birthweight below 1000\u00a0g with clinical suspicion of late-onset sepsis. The approach reached the high sensitivities demanded in the clinical scenario (LOD<jats:sub>PCT<\/jats:sub>\u2009=\u20090.003\u00a0ng\/mL, LOD<jats:sub>IL6<\/jats:sub>\u2009=\u20090.15\u00a0pg\/mL) with excellent correlation performance (<jats:italic>r<\/jats:italic>\u2009&gt;\u20090.9990, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) of the MM-based approach with the Hospital method for both biomarkers during the analysis of diagnosed samples and reliability (Er\u2009&lt;\u20096% for PCT, and Er\u2009&lt;\u20094% for IL-6). The proposed approach also encompasses distinctive technical attributes in a clinical scenario since its minimal sample volume requirements and expeditious results compatible with few easy-to-obtain drops of heel stick blood samples from newborns admitted to the neonatal intensive care unit. This\u00a0would enable the monitoring of both sepsis biomarkers within the initial hours after the manifestation of symptoms in high-risk neonates as a valuable tool in facilitating prompt and well-informed decisions about the initiation of antibiotic therapy.<\/jats:p><jats:p>These results revealed the asset behind micromotor technology for multiplexing analysis in diagnosing neonatal sepsis, opening new avenues in low sample volume-based diagnostics.<\/jats:p>\n                <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s00604-023-06134-x","type":"journal-article","created":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T12:02:41Z","timestamp":1705665761000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Micromotor-based dual aptassay for early cost-effective diagnosis of neonatal sepsis"],"prefix":"10.1007","volume":"191","author":[{"given":"Jos\u00e9 M.","family":"Gord\u00f3n Pidal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Arruza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Moreno-Guzm\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel \u00c1ngel","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7302-0948","authenticated-orcid":false,"given":"Alberto","family":"Escarpa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,19]]},"reference":[{"key":"6134_CR1","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1007\/s00134-003-1662-x","volume":"29","author":"MM Levy","year":"2003","unstructured":"Levy MM, Fink MP, Marshall JC et al (2003) 2001 SCCM\/ESICM\/ACCP\/ATS\/SIS international sepsis definitions conference. 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