{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T06:09:18Z","timestamp":1793858958739,"version":"4.6.1"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T00:00:00Z","timestamp":1715644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T00:00:00Z","timestamp":1715644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82102502"],"award-info":[{"award-number":["82102502"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010903","name":"Key Programme","doi-asserted-by":"publisher","award":["82030065"],"award-info":[{"award-number":["82030065"]}],"id":[{"id":"10.13039\/501100010903","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071833"],"award-info":[{"award-number":["82071833"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microchim Acta"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1007\/s00604-024-06395-0","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T09:02:16Z","timestamp":1715677336000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Nucleic acid aptamer-based electrochemical sensor for the detection of serum P-tau231 and the instant screening test of Alzheimer\u2019s disease"],"prefix":"10.1007","volume":"191","author":[{"given":"Qingfei","family":"Kong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanlin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5055-1423","authenticated-orcid":false,"given":"Feiyun","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,14]]},"reference":[{"key":"6395_CR1","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.1016\/S0140-6736(20)32205-4","volume":"397","author":"P Scheltens","year":"2021","unstructured":"Scheltens P, De Strooper B, Kivipelto M et al (2021) Alzheimer\u2019s disease. The Lancet 397:1577\u20131590. https:\/\/doi.org\/10.1016\/S0140-6736(20)32205-4","journal-title":"The Lancet"},{"key":"6395_CR2","doi-asserted-by":"publisher","first-page":"e661","DOI":"10.1016\/S2468-2667(20)30185-7","volume":"5","author":"L Jia","year":"2020","unstructured":"Jia L, Du Y, Chu L et al (2020) Prevalence, risk factors, and management of dementia and mild cognitive impairment in adults aged 60 years or older in China: a cross-sectional study. Lancet Public Health 5:e661\u2013e671. https:\/\/doi.org\/10.1016\/S2468-2667(20)30185-7","journal-title":"Lancet Public Health"},{"key":"6395_CR3","doi-asserted-by":"publisher","DOI":"10.1136\/gpsych-2022-100751","volume":"35","author":"R Ren","year":"2022","unstructured":"Ren R, Qi J, Lin S et al (2022) The China Alzheimer Report 2022. Gen Psychiatry 35:e100751. https:\/\/doi.org\/10.1136\/gpsych-2022-100751","journal-title":"Gen Psychiatry"},{"key":"6395_CR4","doi-asserted-by":"publisher","unstructured":"(2023) 2023 Alzheimer\u2019s disease facts and figures. Alzheimers Dement 19:1598\u20131695. https:\/\/doi.org\/10.1002\/alz.13016","DOI":"10.1002\/alz.13016"},{"key":"6395_CR5","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.ijbiomac.2019.04.120","volume":"133","author":"A Venkatramani","year":"2019","unstructured":"Venkatramani A, Panda D (2019) Regulation of neuronal microtubule dynamics by tau: implications for tauopathies. Int J Biol Macromol 133:473\u2013483. https:\/\/doi.org\/10.1016\/j.ijbiomac.2019.04.120","journal-title":"Int J Biol Macromol"},{"key":"6395_CR6","doi-asserted-by":"publisher","first-page":"14537","DOI":"10.1523\/JNEUROSCI.2692-08.2008","volume":"28","author":"D Puzzo","year":"2008","unstructured":"Puzzo D, Privitera L, Leznik E et al (2008) Picomolar amyloid-\u03b2 positively modulates synaptic plasticity and memory in hippocampus. J Neurosci 28:14537\u201314545. https:\/\/doi.org\/10.1523\/JNEUROSCI.2692-08.2008","journal-title":"J Neurosci"},{"key":"6395_CR7","doi-asserted-by":"publisher","first-page":"2460","DOI":"10.3390\/ijms22052460","volume":"22","author":"R Pluta","year":"2021","unstructured":"Pluta R, Ouyang L, Januszewski S et al (2021) Participation of amyloid and tau protein in post-ischemic neurodegeneration of the hippocampus of a nature identical to Alzheimer\u2019s disease. Int J Mol Sci 22:2460. https:\/\/doi.org\/10.3390\/ijms22052460","journal-title":"Int J Mol Sci"},{"key":"6395_CR8","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1001\/jamaneurol.2022.4485","volume":"80","author":"J Therriault","year":"2023","unstructured":"Therriault J, Vermeiren M, Servaes S et al (2023) Association of phosphorylated tau biomarkers with amyloid positron emission tomography vs tau positron emission tomography. JAMA Neurol 80:188. https:\/\/doi.org\/10.1001\/jamaneurol.2022.4485","journal-title":"JAMA Neurol"},{"key":"6395_CR9","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1001\/jamaneurol.2021.4222","volume":"79","author":"MC Gonzalez","year":"2022","unstructured":"Gonzalez MC, Ashton NJ, Gomes BF et al (2022) Association of plasma p-tau181 and p-tau231 concentrations with cognitive decline in patients with probable dementia with Lewy bodies. JAMA Neurol 79:32. https:\/\/doi.org\/10.1001\/jamaneurol.2021.4222","journal-title":"JAMA Neurol"},{"key":"6395_CR10","doi-asserted-by":"publisher","first-page":"e12921","DOI":"10.15252\/emmm.202012921","volume":"12","author":"M Su\u00e1rez-Calvet","year":"2020","unstructured":"Su\u00e1rez-Calvet M, Karikari TK, Ashton NJ et al (2020) Novel tau biomarkers phosphorylated at T181, T217 or T231 rise in the initial stages of the preclinical Alzheimer\u2019s continuum when only subtle changes in A\u03b2 pathology are detected. EMBO Mol Med 12:e12921. https:\/\/doi.org\/10.15252\/emmm.202012921","journal-title":"EMBO Mol Med"},{"key":"6395_CR11","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1186\/s40478-018-0557-6","volume":"6","author":"J Neddens","year":"2018","unstructured":"Neddens J, Temmel M, Flunkert S et al (2018) Phosphorylation of different tau sites during progression of Alzheimer\u2019s disease. Acta Neuropathol Commun 6:52. https:\/\/doi.org\/10.1186\/s40478-018-0557-6","journal-title":"Acta Neuropathol Commun"},{"key":"6395_CR12","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.jalz.2013.02.005","volume":"10","author":"RLH Handels","year":"2014","unstructured":"Handels RLH, Wolfs CAG, Aalten P et al (2014) Diagnosing Alzheimer\u2019s disease: a systematic review of economic evaluations. Alzheimers Dement 10:225\u2013237. https:\/\/doi.org\/10.1016\/j.jalz.2013.02.005","journal-title":"Alzheimers Dement"},{"key":"6395_CR13","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1002\/alz.12447","volume":"18","author":"P Chatterjee","year":"2022","unstructured":"Chatterjee P, Pedrini S, Ashton NJ et al (2022) Diagnostic and prognostic plasma biomarkers for preclinical Alzheimer\u2019s disease. Alzheimers Dement 18:1141\u20131154. https:\/\/doi.org\/10.1002\/alz.12447","journal-title":"Alzheimers Dement"},{"key":"6395_CR14","doi-asserted-by":"publisher","first-page":"2555","DOI":"10.1038\/s41591-022-02074-w","volume":"28","author":"NJ Ashton","year":"2022","unstructured":"Ashton NJ, Janelidze S, Mattsson-Carlgren N et al (2022) Differential roles of A\u03b242\/40, p-tau231 and p-tau217 for Alzheimer\u2019s trial selection and disease monitoring. Nat Med 28:2555\u20132562. https:\/\/doi.org\/10.1038\/s41591-022-02074-w","journal-title":"Nat Med"},{"key":"6395_CR15","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1111\/cns.12814","volume":"24","author":"S Ki\u0111emet-Piska\u010d","year":"2018","unstructured":"Ki\u0111emet-Piska\u010d S, Babi\u0107 Leko M, Bla\u017eekovi\u0107 A et al (2018) Evaluation of cerebrospinal fluid phosphorylated tau 231 as a biomarker in the differential diagnosis of Alzheimer\u2019s disease and vascular dementia. CNS Neurosci Ther 24:734\u2013740. https:\/\/doi.org\/10.1111\/cns.12814","journal-title":"CNS Neurosci Ther"},{"key":"6395_CR16","doi-asserted-by":"publisher","unstructured":"Ashton NJ, Puig\u2010Pijoan A, Mil\u00e0\u2010Alom\u00e0 M, et al (2022) Plasma and CSF biomarkers in a memory clinic: head\u2010to\u2010head comparison of phosphorylated tau immunoassays. Alzheimers Dement alz.12841. https:\/\/doi.org\/10.1002\/alz.12841","DOI":"10.1002\/alz.12841"},{"key":"6395_CR17","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-01925-w","author":"M Mil\u00e0-Alom\u00e0","year":"2022","unstructured":"Mil\u00e0-Alom\u00e0 M, Ashton NJ, Shekari M et al (2022) Plasma p-tau231 and p-tau217 as state markers of amyloid-\u03b2 pathology in preclinical Alzheimer\u2019s disease. Nat Med. https:\/\/doi.org\/10.1038\/s41591-022-01925-w","journal-title":"Nat Med"},{"key":"6395_CR18","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1002\/ana.26308","volume":"91","author":"P Meyer","year":"2022","unstructured":"Meyer P, Ashton NJ, Karikari TK et al (2022) Plasma p-tau231, p-tau181, PET biomarkers, and cognitive change in older adults. Ann Neurol 91:548\u2013560. https:\/\/doi.org\/10.1002\/ana.26308","journal-title":"Ann Neurol"},{"key":"6395_CR19","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/s13024-023-00605-8","volume":"18","author":"F Gonzalez-Ortiz","year":"2023","unstructured":"Gonzalez-Ortiz F, Kac PR, Brum WS et al (2023) Plasma phospho-tau in Alzheimer\u2019s disease: towards diagnostic and therapeutic trial applications. Mol Neurodegener 18:18. https:\/\/doi.org\/10.1186\/s13024-023-00605-8","journal-title":"Mol Neurodegener"},{"key":"6395_CR20","doi-asserted-by":"publisher","first-page":"942629","DOI":"10.3389\/fnagi.2022.942629","volume":"14","author":"L Chen","year":"2022","unstructured":"Chen L, Niu X, Wang Y et al (2022) Plasma tau proteins for the diagnosis of mild cognitive impairment and Alzheimer\u2019s disease: a systematic review and meta-analysis. Front Aging Neurosci 14:942629. https:\/\/doi.org\/10.3389\/fnagi.2022.942629","journal-title":"Front Aging Neurosci"},{"key":"6395_CR21","doi-asserted-by":"publisher","first-page":"103836","DOI":"10.1016\/j.ebiom.2022.103836","volume":"76","author":"NJ Ashton","year":"2022","unstructured":"Ashton NJ, Benedet AL, Pascoal TA et al (2022) Cerebrospinal fluid p-tau231 as an early indicator of emerging pathology in Alzheimer\u2019s disease. BioMedicine 76:103836. https:\/\/doi.org\/10.1016\/j.ebiom.2022.103836","journal-title":"BioMedicine"},{"key":"6395_CR22","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1007\/s00401-021-02275-6","volume":"141","author":"NJ Ashton","year":"2021","unstructured":"Ashton NJ, Pascoal TA, Karikari TK et al (2021) Plasma p-tau231: a new biomarker for incipient Alzheimer\u2019s disease pathology. Acta Neuropathol (Berl) 141:709\u2013724. https:\/\/doi.org\/10.1007\/s00401-021-02275-6","journal-title":"Acta Neuropathol (Berl)"},{"key":"6395_CR23","doi-asserted-by":"publisher","unstructured":"Graff-Radford J, Yong KXX, Apostolova LG, et al (2021) New insights into atypical Alzheimer\u2019s disease in the era of biomarkers. Lancet Neurol 20:222\u2013234. https:\/\/doi.org\/10.1016\/S1474-4422(20)30440-3","DOI":"10.1016\/S1474-4422(20)30440-3"},{"key":"6395_CR24","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1186\/s13195-022-01011-w","volume":"14","author":"PR Kac","year":"2022","unstructured":"Kac PR, Gonzalez-Ortiz F, Simr\u00e9n J et al (2022) Diagnostic value of serum versus plasma phospho-tau for Alzheimer\u2019s disease. Alzheimers Res Ther 14:65. https:\/\/doi.org\/10.1186\/s13195-022-01011-w","journal-title":"Alzheimers Res Ther"},{"key":"6395_CR25","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1038\/s43587-023-00405-1","volume":"3","author":"L Montoliu-Gaya","year":"2023","unstructured":"Montoliu-Gaya L, Benedet AL, Tissot C et al (2023) Mass spectrometric simultaneous quantification of tau species in plasma shows differential associations with amyloid and tau pathologies. Nat Aging 3:661\u2013669. https:\/\/doi.org\/10.1038\/s43587-023-00405-1","journal-title":"Nat Aging"},{"key":"6395_CR26","doi-asserted-by":"publisher","first-page":"46645","DOI":"10.1021\/acsami.9b19180","volume":"11","author":"JC Soares","year":"2019","unstructured":"Soares JC, Soares AC, Rodrigues VC et al (2019) Detection of the prostate cancer biomarker PCA3 with electrochemical and impedance-based biosensors. ACS Appl Mater Interfaces 11:46645\u201346650. https:\/\/doi.org\/10.1021\/acsami.9b19180","journal-title":"ACS Appl Mater Interfaces"},{"key":"6395_CR27","doi-asserted-by":"publisher","first-page":"2516","DOI":"10.3390\/ijms18122516","volume":"18","author":"A Kinghorn","year":"2017","unstructured":"Kinghorn A, Fraser L, Liang S et al (2017) Aptamer bioinformatics. Int J Mol Sci 18:2516. https:\/\/doi.org\/10.3390\/ijms18122516","journal-title":"Int J Mol Sci"},{"key":"6395_CR28","doi-asserted-by":"publisher","first-page":"1791","DOI":"10.1007\/s00216-018-1273-6","volume":"411","author":"S Campuzano","year":"2019","unstructured":"Campuzano S, Y\u00e1\u00f1ez-Sede\u00f1o P, Pingarr\u00f3n JM (2019) Nanoparticles for nucleic-acid-based biosensing: opportunities, challenges, and prospects. Anal Bioanal Chem 411:1791\u20131806. https:\/\/doi.org\/10.1007\/s00216-018-1273-6","journal-title":"Anal Bioanal Chem"},{"key":"6395_CR29","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1146\/annurev-anchem-061417-125655","volume":"14","author":"KR Wehmeyer","year":"2021","unstructured":"Wehmeyer KR, White RJ, Kissinger PT, Heineman WR (2021) Electrochemical affinity assays\/sensors: brief history and current status. Annu Rev Anal Chem 14:109\u2013131. https:\/\/doi.org\/10.1146\/annurev-anchem-061417-125655","journal-title":"Annu Rev Anal Chem"},{"key":"6395_CR30","doi-asserted-by":"publisher","first-page":"20388","DOI":"10.1021\/acsami.1c04933","volume":"13","author":"Y Xu","year":"2021","unstructured":"Xu Y, Wang X, Ding C, Luo X (2021) Ratiometric antifouling electrochemical biosensors based on multifunctional peptides and MXene loaded with Au nanoparticles and methylene blue. ACS Appl Mater Interfaces 13:20388\u201320396. https:\/\/doi.org\/10.1021\/acsami.1c04933","journal-title":"ACS Appl Mater Interfaces"},{"key":"6395_CR31","doi-asserted-by":"publisher","first-page":"120210","DOI":"10.1016\/j.talanta.2019.120210","volume":"206","author":"T Xiao","year":"2020","unstructured":"Xiao T, Huang J, Wang D et al (2020) Au and Au-based nanomaterials: synthesis and recent progress in electrochemical sensor applications. Talanta 206:120210. https:\/\/doi.org\/10.1016\/j.talanta.2019.120210","journal-title":"Talanta"},{"key":"6395_CR32","doi-asserted-by":"publisher","first-page":"93","DOI":"10.3390\/biomedicines10010093","volume":"10","author":"LMT Phan","year":"2022","unstructured":"Phan LMT, Cho S (2022) Fluorescent aptasensor and colorimetric aptablot for p-tau231 detection: toward early diagnosis of Alzheimer\u2019s disease. Biomedicines 10:93. https:\/\/doi.org\/10.3390\/biomedicines10010093","journal-title":"Biomedicines"},{"key":"6395_CR33","doi-asserted-by":"publisher","first-page":"14314","DOI":"10.1021\/jacs.8b08645","volume":"140","author":"I-T Teng","year":"2018","unstructured":"Teng I-T, Li X, Yadikar HA et al (2018) Identification and characterization of DNA aptamers specific for phosphorylation epitopes of tau protein. J Am Chem Soc 140:14314\u201314323. https:\/\/doi.org\/10.1021\/jacs.8b08645","journal-title":"J Am Chem Soc"},{"key":"6395_CR34","doi-asserted-by":"publisher","first-page":"1598","DOI":"10.1021\/acs.analchem.9b04919","volume":"92","author":"W Wang","year":"2020","unstructured":"Wang W, Liu Y, Shi T et al (2020) Biosynthesized quantum dot for facile and ultrasensitive electrochemical and electrochemiluminescence immunoassay. Anal Chem 92:1598\u20131604. https:\/\/doi.org\/10.1021\/acs.analchem.9b04919","journal-title":"Anal Chem"},{"key":"6395_CR35","doi-asserted-by":"publisher","first-page":"10","DOI":"10.3390\/biomedicines10010010","volume":"10","author":"HTN Le","year":"2021","unstructured":"Le HTN, Cho S (2021) Sensitive electrochemical detection of phosphorylated-tau threonine 231 in human serum using interdigitated wave-shaped electrode. Biomedicines 10:10. https:\/\/doi.org\/10.3390\/biomedicines10010010","journal-title":"Biomedicines"}],"container-title":["Microchimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-024-06395-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00604-024-06395-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-024-06395-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T10:09:52Z","timestamp":1718014192000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00604-024-06395-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,14]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["6395"],"URL":"https:\/\/doi.org\/10.1007\/s00604-024-06395-0","relation":{},"ISSN":["0026-3672","1436-5073"],"issn-type":[{"value":"0026-3672","type":"print"},{"value":"1436-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,14]]},"assertion":[{"value":"14 January 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 April 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All authors contributed to the article and approved the submitted version.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"328"}}