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The electrochemical sensor was based on lab-printed carbon electrodes (C-PE) enriched with cauliflower-shaped gold nanostructures (AuNSs), on which VAN-specific aptamers were immobilized as biorecognition elements and short-chain thiols as blocking agents. The AuNSs, characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM), enhanced the electrochemical properties of the platform and the aptamer immobilization active sites. The developed disposable aptasensor allowed label-free detection of VAN via electrochemical impedance spectroscopy (EIS) across a wide range of concentrations (50\u20131000 nM), with a limit of detection (LOD) of 1.721 nM. The aptasensor presented good selectivity against some commonly found interferences in human serum and milk and was successfully applied to\u00a0the analysis of these samples.<\/jats:p>\n          <jats:p>\n            <jats:bold>Graphical Abstract<\/jats:bold>\n          <\/jats:p>","DOI":"10.1007\/s00604-025-06952-1","type":"journal-article","created":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T18:28:54Z","timestamp":1737829734000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Electrochemical aptasensor for the selective detection of vancomycin based on nanostructured \u201cin-lab\u201d printed electrodes"],"prefix":"10.1007","volume":"192","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0950-0875","authenticated-orcid":false,"given":"Malek","family":"Bibani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0070-4142","authenticated-orcid":false,"given":"Magdolna","family":"Casian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9309-0951","authenticated-orcid":false,"given":"Bogdan","family":"Feier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5046-4837","authenticated-orcid":false,"given":"Diana","family":"Bogdan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3579-5195","authenticated-orcid":false,"given":"Oana","family":"Hosu-Stancioiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9823-9818","authenticated-orcid":false,"given":"Nadia","family":"Ktari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6638-9133","authenticated-orcid":false,"given":"Rafik","family":"Kalfat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4158-3324","authenticated-orcid":false,"given":"Cecilia","family":"Cristea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,25]]},"reference":[{"issue":"9","key":"6952_CR1","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1038\/ja.2014.111","volume":"67","author":"MS Butler","year":"2014","unstructured":"Butler MS, Hansford KA, Blaskovich MAT, Halai R, Cooper MA (2014) Glycopeptide antibiotics: back to the future. 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