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There is an urgent need for less invasive, cost-effective diagnostic methods that can reliably detect ND-related biomarkers in readily available biofluids such as serum. In response to this need, we have developed an ultra-sensitive assay that utilizes magnetic nanoparticles and a novel, tailor-made turn-on fluorescent probe named F-SPG. This innovative assay enables the detection of neurofilament light chain (NfL) at femtomolar concentrations, with a remarkable detection limit of 24 fM which is at least 18 times more sensitive than the conventional ELISA kits. Our assay\u2019s design eliminates the need for traditional secondary antibody in immunoassay, thereby streamlining the diagnostic process. Recoveries exceeding 95% underscore the assay\u2019s precision and reliability. It has proven effective in distinguishing Alzheimer\u2019s Disease patients from healthy individuals by quantifying serum NfL levels, showcasing its potential as a cost-effective, ultra-sensitive tool for the early screening of neurodegenerative diseases.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1007\/s00604-025-06995-4","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T16:38:26Z","timestamp":1738687106000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A turn-on fluorescent immunosensor for neurodegenerative disease related neurofilament light chain protein"],"prefix":"10.1007","volume":"192","author":[{"given":"Qingting","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man Shing","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung-Wing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,4]]},"reference":[{"key":"6995_CR1","unstructured":"Attia P (2023) Outlive: the science and art of longevity. 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