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The molecularly imprinted polymer (MIP)\u2013based sensor (CIN@MIP\/GCE) was electropolymerized using cyclic voltammetry (CV) of the functional monomer o-phenylenediamine (o\u2013PD) with a template molecule CIN on a glassy carbon electrode (GCE). The optimum performance of the MIP-based electrode for CIN detection was obtained with parameters of a 1:7 monomer ratio, a 15-min removal time, ethanol as a removal solution, and a 15-min rebinding time. The surface characterization of the CIN@MIP\/GCE sensor was conducted using atomic force microscopy (AFM) and scanning electron microscopy (SEM), while CV and electrochemical impedance spectroscopy (EIS) were employed for electrochemical characterization with [Fe(CN)<jats:sub>6<\/jats:sub>]<jats:sup>3\u2212<\/jats:sup>\/<jats:sup>4\u2212<\/jats:sup> redox probe. AFM findings show that the MIP sensor with CIN-specific voids on the surface has a root-mean-square (RMS) value of 27.95, while the non-imprinted polymer (NIP) sensor without voids has a smoother surface formation and an RMS value of 21.30\u00a0nm. The analytical efficacy of the constructed sensor was assessed using differential pulse voltammetry (DPV). The limit of detection (LOD) was 0.17\u2009\u00d7\u200910<jats:sup>\u221212<\/jats:sup>, with a linear range of 1.0\u2009\u00d7\u200910<jats:sup>\u221212<\/jats:sup>\u20131.0\u2009\u00d7\u200910<jats:sup>\u221211<\/jats:sup>\u00a0M. The reliability of the constructed sensor was determined using CIN detection in real samples as tablet dosage form and human serum, yielding recovery results of 100.19% and 101.82%, respectively. The selectivity investigation was performed against prevalent interference substances. The relative imprinting factor (IF) values of CIN impurities confirmed the selectivity of the CIN sensor.<\/jats:p>\n          <jats:p>\n            <jats:bold>Graphical Abstract<\/jats:bold>\n          <\/jats:p>","DOI":"10.1007\/s00604-025-07152-7","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T15:47:09Z","timestamp":1744732029000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A novel electrochemical sensor based on MIP technology for sensitive determination of cinacalcet hydrochloride in tablet dosage form and serum samples"],"prefix":"10.1007","volume":"192","author":[{"given":"Ipek","family":"Kucuk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selenay","family":"Sadak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selda Zengin","family":"Kurnal\u0131","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sacide","family":"Alt\u0131n\u00f6z","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bengi","family":"Uslu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"7152_CR1","doi-asserted-by":"publisher","first-page":"271","DOI":"10.2165\/00003495-200565020-00007","volume":"65","author":"JAB Balfour","year":"2005","unstructured":"Balfour JAB, Scott LJ (2005) Cinacalcet hydrochloride. 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