{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T18:31:16Z","timestamp":1774031476456,"version":"3.50.1"},"reference-count":78,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100019336","name":"Key Research and Development Plan of Tianjin","doi-asserted-by":"publisher","award":["21JCZDJC00580"],"award-info":[{"award-number":["21JCZDJC00580"]}],"id":[{"id":"10.13039\/501100019336","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microchim Acta"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1007\/s00604-025-07451-z","type":"journal-article","created":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T10:35:44Z","timestamp":1755599744000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Paper-based and chemical-free dual low-cost electrochemical biosensor for rapid detection of Escherichia coli O157:H7"],"prefix":"10.1007","volume":"192","author":[{"given":"Manman","family":"Du","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junting","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianteng","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinwu","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Can","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,8,19]]},"reference":[{"key":"7451_CR1","unstructured":"WHO, UNICEF, World Bank (2022) State of the world\u2019s drinking water: an urgent call to action to accelerate progress on ensuring safe drinking water for all. Licence: CC BY-NC-SA 3.0 IG https:\/\/www.who.int\/publications\/i\/item\/9789240060807. Accessed 24 Oct 2022"},{"key":"7451_CR2","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s11157-014-9351-5","volume":"14","author":"K Amini","year":"2015","unstructured":"Amini K, Kraatz HB (2015) Recent advances and developments in monitoring biological agents in water samples. Rev Environ Sci Biotechnol 14:23\u201348. https:\/\/doi.org\/10.1007\/s11157-014-9351-5","journal-title":"Rev Environ Sci Biotechnol"},{"issue":"20","key":"7451_CR3","doi-asserted-by":"publisher","DOI":"10.3390\/s19204476","volume":"19","author":"S Kumar","year":"2019","unstructured":"Kumar S, Nehra M, Mehta J, Dilbaghi N, Marrazza G, Kaushik A (2019) Point-of-care strategies for detection of waterborne pathogens. Sensors (Basel) 19(20):4476. https:\/\/doi.org\/10.3390\/s19204476","journal-title":"Sensors (Basel)"},{"key":"7451_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2021.109233","volume":"349","author":"A Rani","year":"2021","unstructured":"Rani A, Ravindran VB, Surapaneni A, Mantri N, Ball AS (2021) Trends in point-of-care diagnosis for Escherichia coli O157: H7 in food and water. Int J Food Microbiol 349:109233. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2021.109233","journal-title":"Int J Food Microbiol"},{"issue":"5","key":"7451_CR5","doi-asserted-by":"publisher","DOI":"10.3390\/bios10050054","volume":"10","author":"N Razmi","year":"2020","unstructured":"Razmi N, Hasanzadeh M, Willander M, Nur O (2020) Recent progress on the electrochemical biosensing of Escherichia coli O157: H7: material and methods overview. Biosensors 10(5):54. https:\/\/doi.org\/10.3390\/bios10050054","journal-title":"Biosensors"},{"issue":"2","key":"7451_CR6","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1039\/C8AN01488D","volume":"144","author":"P Rajapaksha","year":"2019","unstructured":"Rajapaksha P, Elbourne A, Gangadoo S, Brown R, Cozzolino D, Chapman J (2019) A review of methods for the detection of pathogenic microorganisms. Analyst 144(2):396\u2013411. https:\/\/doi.org\/10.1039\/C8AN01488D","journal-title":"Analyst"},{"issue":"6","key":"7451_CR7","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1002\/mbo3.383","volume":"5","author":"RA Deshmukh","year":"2016","unstructured":"Deshmukh RA, Joshi K, Bhand S, Roy U (2016) Recent developments in detection and enumeration of waterborne bacteria: a retrospective minireview. Microbiol Open 5(6):901\u2013922. https:\/\/doi.org\/10.1002\/mbo3.383","journal-title":"Microbiol Open"},{"key":"7451_CR8","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2019.111752","volume":"147","author":"Y Li","year":"2020","unstructured":"Li Y, Liu H, Huang H, Deng J, Fang L, Luo J, Zhang S, Huang J, Liang W, Zheng J (2020) A sensitive electrochemical strategy via multiple amplification reactions for the detection of E. coli O157: H7. Biosens Bioelectron 147:111752. https:\/\/doi.org\/10.1016\/j.bios.2019.111752","journal-title":"Biosens Bioelectron"},{"issue":"1","key":"7451_CR9","doi-asserted-by":"publisher","DOI":"10.1038\/s41545-024-00368-9","volume":"7","author":"M Khodaparast","year":"2024","unstructured":"Khodaparast M, Sharley D, Marshall S, Beddoe T (2024) Advances in point-of-care and molecular techniques to detect waterborne pathogens. NPJ Clean Water 7(1):74. https:\/\/doi.org\/10.1038\/s41545-024-00368-9","journal-title":"NPJ Clean Water"},{"issue":"Supplement_2","key":"7451_CR10","doi-asserted-by":"publisher","first-page":"S84","DOI":"10.1093\/cid\/ciaa498","volume":"71","author":"J Li","year":"2020","unstructured":"Li J, Zhu Y, Wu X, Hoffmann MR (2020) Rapid detection methods for bacterial pathogens in ambient waters at the point of sample collection: a brief review. Clin Infect Dis 71(Supplement_2):S84\u2013S90. https:\/\/doi.org\/10.1093\/cid\/ciaa498","journal-title":"Clin Infect Dis"},{"issue":"9","key":"7451_CR11","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1038\/s41557-021-00718-x","volume":"13","author":"R Pandey","year":"2021","unstructured":"Pandey R, Chang D, Smieja M, Hoare T, Li Y, Soleymani L (2021) Integrating programmable DNAzymes with electrical readout for rapid and culture-free bacterial detection using a handheld platform. Nat Chem 13(9):895\u2013901. https:\/\/doi.org\/10.1038\/s41557-021-00718-x","journal-title":"Nat Chem"},{"issue":"24","key":"7451_CR12","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics14242876","volume":"14","author":"AN Alsharksi","year":"2024","unstructured":"Alsharksi AN, Sirekbasan S, G\u00fcrk\u00f6k-Tan T, Mustapha A (2024) From tradition to innovation: diverse molecular techniques in the fight against infectious diseases. Diagnostics 14(24):2876. https:\/\/doi.org\/10.3390\/diagnostics14242876","journal-title":"Diagnostics"},{"key":"7451_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2024.114767","volume":"193","author":"R Gao","year":"2024","unstructured":"Gao R, Liu X, Xiong Z, Wang G, Ai L (2024) Research progress on detection of foodborne pathogens: the more rapid and accurate answer to food safety. Food Res Int 193:114767. https:\/\/doi.org\/10.1016\/j.foodres.2024.114767","journal-title":"Food Res Int"},{"issue":"7","key":"7451_CR14","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1080\/14737159.2022.2117615","volume":"22","author":"R Ahirwar","year":"2022","unstructured":"Ahirwar R, Bhattacharya A, Kumar S (2022) Unveiling the underpinnings of various non-conventional ELISA variants: a review article. Expert Rev Mol Diagn 22(7):761\u2013774. https:\/\/doi.org\/10.1080\/14737159.2022.2117615","journal-title":"Expert Rev Mol Diagn"},{"issue":"6","key":"7451_CR15","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1016\/j.jscs.2014.09.001","volume":"19","author":"M Sajid","year":"2015","unstructured":"Sajid M, Kawde A-N, Daud M (2015) Designs, formats and applications of lateral flow assay: a literature review. J Saudi Chem Soc 19(6):689\u2013705. https:\/\/doi.org\/10.1016\/j.jscs.2014.09.001","journal-title":"J Saudi Chem Soc"},{"key":"7451_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112214","volume":"159","author":"E Cesewski","year":"2020","unstructured":"Cesewski E, Johnson BN (2020) Electrochemical biosensors for pathogen detection. Biosens Bioelectron 159:112214. https:\/\/doi.org\/10.1016\/j.bios.2020.112214","journal-title":"Biosens Bioelectron"},{"key":"7451_CR17","doi-asserted-by":"publisher","DOI":"10.1016\/j.jelechem.2021.114989","volume":"882","author":"HO Kaya","year":"2021","unstructured":"Kaya HO, Cetin AE, Azimzadeh M, Topkaya SN (2021) Pathogen detection with electrochemical biosensors: advantages, challenges and future perspectives. J Electroanal Chem 882:114989. https:\/\/doi.org\/10.1016\/j.jelechem.2021.114989","journal-title":"J Electroanal Chem"},{"issue":"12","key":"7451_CR18","doi-asserted-by":"publisher","first-page":"7737","DOI":"10.1007\/s11696-023-03035-4","volume":"77","author":"A Hyder","year":"2023","unstructured":"Hyder A, Memon SS, Buledi JA, Memon S, Memon Z-u-A, Shaikh SG, Rajpar DB (2023) Strategic fabrication of para diethylamine calix[4]arene-functionalized CuO nanostructures: a metal-organic framework-based sensor for quantification of antipyrine. Chem Pap 77(12):7737\u20137748. https:\/\/doi.org\/10.1007\/s11696-023-03035-4","journal-title":"Chem Pap"},{"key":"7451_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.materresbull.2025.113386","volume":"188","author":"SA Solangi","year":"2025","unstructured":"Solangi SA, Baig JA, Solangi IB, Afridi HI, Hussain S, Akhtar K, Khan LU, Abbasi F, Hyder A (2025) Bismuth ferrite polyaniline nanohybrid based electrochemical detection of theophylline. Mater Res Bull 188:113386. https:\/\/doi.org\/10.1016\/j.materresbull.2025.113386","journal-title":"Mater Res Bull"},{"key":"7451_CR20","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2020.105908","volume":"163","author":"A Hyder","year":"2021","unstructured":"Hyder A, Sanam Memon S, Memon S, Sirajuddin M-u-A, Bux Rajpar D, Gul Shaikh S, Ahmed Buledi J (2021) A highly discerning p-tetranitrocalix[4]arene (p-TNC4) functionalized copper nanoparticles: a smart electrochemical sensor for the selective determination of Diphenhydramine drug. Microchem J 163:105908. https:\/\/doi.org\/10.1016\/j.microc.2020.105908","journal-title":"Microchem J"},{"issue":"5","key":"7451_CR21","doi-asserted-by":"publisher","first-page":"2160","DOI":"10.1039\/d4su00828f","volume":"3","author":"A Hyder","year":"2025","unstructured":"Hyder A, Khilji M-U-N, Buledi JA, Memon AA, Ghanghro A, ur Rehman M, Thebo KH (2025) MXene-based nanocomposites: a new horizon for electrochemical monitoring of environmental pollutants. RSC Sustain 3(5):2160\u20132184. https:\/\/doi.org\/10.1039\/d4su00828f","journal-title":"RSC Sustain"},{"key":"7451_CR22","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2023.111934","volume":"160","author":"JA Buledi","year":"2024","unstructured":"Buledi JA, Hyder A, Solangi AR, Shah Z-u-H, Darabi R, Karimi-Maleh H (2024) Nano-diamonds: transformative nanoscale material in advancing biosensor technology. Inorg Chem Commun 160:111934. https:\/\/doi.org\/10.1016\/j.inoche.2023.111934","journal-title":"Inorg Chem Commun"},{"issue":"14","key":"7451_CR23","doi-asserted-by":"publisher","first-page":"10940","DOI":"10.1039\/D4CP00138A","volume":"26","author":"A Hyder","year":"2024","unstructured":"Hyder A, Ali A, Buledi JA, Memon R, Al-Anzi BS, Memon AA, Kazi M, Solangi AR, Yang J, Thebo KH (2024) A NiO-nanostructure-based electrochemical sensor functionalized with supramolecular structures for the ultra-sensitive detection of the endocrine disruptor bisphenol S in an aquatic environment. Phys Chem Chem Phys 26(14):10940\u201310950. https:\/\/doi.org\/10.1039\/D4CP00138A","journal-title":"Phys Chem Chem Phys"},{"issue":"22","key":"7451_CR24","doi-asserted-by":"publisher","first-page":"6461","DOI":"10.1039\/C9AN01747J","volume":"144","author":"O Simoska","year":"2019","unstructured":"Simoska O, Stevenson KJ (2019) Electrochemical sensors for rapid diagnosis of pathogens in real time. Analyst 144(22):6461\u20136478. https:\/\/doi.org\/10.1039\/C9AN01747J","journal-title":"Analyst"},{"key":"7451_CR25","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.135906","volume":"417","author":"H Zhou","year":"2023","unstructured":"Zhou H, Guo W, Hao T, Xie J, Wu Y, Jiang X, Hu Y, Wang S, Guo Z (2023) Electrochemical sensor for single-cell determination of bacteria based on target-triggered click chemistry and fast scan voltammetry. Food Chem 417:135906. https:\/\/doi.org\/10.1016\/j.foodchem.2023.135906","journal-title":"Food Chem"},{"issue":"7","key":"7451_CR26","doi-asserted-by":"publisher","DOI":"10.3390\/bios10070076","volume":"10","author":"P Y\u00e1\u00f1ez-Sede\u00f1o","year":"2020","unstructured":"Y\u00e1\u00f1ez-Sede\u00f1o P, Campuzano S, Pingarr\u00f3n JM (2020) Screen-printed electrodes: promising paper and wearable transducers for (bio) sensing. Biosensors 10(7):76. https:\/\/doi.org\/10.3390\/bios10070076","journal-title":"Biosensors"},{"issue":"5","key":"7451_CR27","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1038\/s44222-023-00032-w","volume":"1","author":"J Wu","year":"2023","unstructured":"Wu J, Liu H, Chen W, Ma B, Ju H (2023) Device integration of electrochemical biosensors. Nat Rev Bioeng 1(5):346\u2013360. https:\/\/doi.org\/10.1038\/s44222-023-00032-w","journal-title":"Nat Rev Bioeng"},{"issue":"15","key":"7451_CR28","doi-asserted-by":"publisher","first-page":"4991","DOI":"10.1039\/C5AN00637F","volume":"140","author":"J Chen","year":"2015","unstructured":"Chen J, Jiang Z, Ackerman JD, Yazdani M, Hou S, Nugen SR, Rotello VM (2015) Electrochemical nanoparticle-enzyme sensors for screening bacterial contamination in drinking water. Analyst 140(15):4991\u20134996. https:\/\/doi.org\/10.1039\/C5AN00637F","journal-title":"Analyst"},{"key":"7451_CR29","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1016\/j.bios.2018.11.001","volume":"126","author":"M Zhong","year":"2019","unstructured":"Zhong M, Yang L, Yang H, Cheng C, Deng W, Tan Y, Xie Q, Yao S (2019) An electrochemical immunobiosensor for ultrasensitive detection of Escherichia coli O157:H7 using CdS quantum dots-encapsulated metal-organic frameworks as signal-amplifying tags. Biosens Bioelectron 126:493\u2013500. https:\/\/doi.org\/10.1016\/j.bios.2018.11.001","journal-title":"Biosens Bioelectron"},{"issue":"1","key":"7451_CR30","doi-asserted-by":"publisher","DOI":"10.3390\/bios13010080","volume":"13","author":"A Mohammadpour-Haratbar","year":"2023","unstructured":"Mohammadpour-Haratbar A, Boraei SBA, Zare Y, Rhee KY, Park SJ (2023) Graphene-based electrochemical biosensors for breast cancer detection. Biosensors 13(1):80. https:\/\/doi.org\/10.3390\/bios13010080","journal-title":"Biosensors"},{"key":"7451_CR31","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.bios.2015.07.002","volume":"76","author":"Y Song","year":"2016","unstructured":"Song Y, Luo Y, Zhu C, Li H, Du D, Lin Y (2016) Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials. Biosens Bioelectron 76:195\u2013212. https:\/\/doi.org\/10.1016\/j.bios.2015.07.002","journal-title":"Biosens Bioelectron"},{"issue":"10","key":"7451_CR32","doi-asserted-by":"publisher","DOI":"10.3390\/s17102161","volume":"17","author":"P Suvarnaphaet","year":"2017","unstructured":"Suvarnaphaet P, Pechprasarn S (2017) Graphene-based materials for biosensors: a review. Sensors (Basel) 17(10):2161. https:\/\/doi.org\/10.3390\/s17102161","journal-title":"Sensors (Basel)"},{"key":"7451_CR33","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.trac.2017.04.003","volume":"91","author":"CI Justino","year":"2017","unstructured":"Justino CI, Gomes AR, Freitas AC, Duarte AC, Rocha-Santos TA (2017) Graphene based sensors and biosensors. TrAC Trends Anal Chem 91:53\u201366. https:\/\/doi.org\/10.1016\/j.trac.2017.04.003","journal-title":"TrAC Trends Anal Chem"},{"key":"7451_CR34","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1016\/j.jpowsour.2011.10.016","volume":"199","author":"KW Park","year":"2012","unstructured":"Park KW, Jung JH (2012) Spectroscopic and electrochemical characteristics of a carboxylated graphene-ZnO composites. J Power Sources 199:379\u2013385. https:\/\/doi.org\/10.1016\/j.jpowsour.2011.10.016","journal-title":"J Power Sources"},{"key":"7451_CR35","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochem.2025.116438","volume":"467","author":"FH Kanhar","year":"2025","unstructured":"Kanhar FH, Pirzada T, Iqbal M, Hyder A, Memon AA, Thebo KH, Kazi M (2025) Efficient visible-light-driven degradation of amoxicillin antibiotic using reduced graphene oxide decorated copper oxide nanocomposite materials. J Photochem Photobiol A 467:116438. https:\/\/doi.org\/10.1016\/j.jphotochem.2025.116438","journal-title":"J Photochem Photobiol A"},{"issue":"3","key":"7451_CR36","doi-asserted-by":"publisher","DOI":"10.3390\/bios15030189","volume":"15","author":"A Fdez-Sanrom\u00e1n","year":"2025","unstructured":"Fdez-Sanrom\u00e1n A, Bern\u00e1rdez-Rodas N, Rosales E, Pazos M, Gonz\u00e1lez-Romero E, Sanrom\u00e1n M\u00c1 (2025) Biosensor technologies for water quality: detection of emerging contaminants and pathogens. Biosensors 15(3):189. https:\/\/doi.org\/10.3390\/bios15030189","journal-title":"Biosensors"},{"issue":"1","key":"7451_CR37","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39701-0","volume":"14","author":"A Maity","year":"2023","unstructured":"Maity A, Pu H, Sui X, Chang J, Bottum KJ, Jin B, Zhou G, Wang Y, Lu G, Chen J (2023) Scalable graphene sensor array for real-time toxins monitoring in flowing water. Nat Commun 14(1):4184. https:\/\/doi.org\/10.1038\/s41467-023-39701-0","journal-title":"Nat Commun"},{"issue":"9","key":"7451_CR38","doi-asserted-by":"publisher","first-page":"3214","DOI":"10.1021\/acssensors.1c00851","volume":"6","author":"S Karuppiah","year":"2021","unstructured":"Karuppiah S, Mishra NC, Tsai W-C, Liao W-S, Chou C-F (2021) Ultrasensitive and low-cost paper-based graphene oxide nanobiosensor for monitoring water-borne bacterial contamination. ACS Sens 6(9):3214\u20133223. https:\/\/doi.org\/10.1021\/acssensors.1c00851","journal-title":"ACS Sens"},{"key":"7451_CR39","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116050","volume":"250","author":"S Mukherjee","year":"2024","unstructured":"Mukherjee S, Mukherjee A, Bytesnikova Z, Ashrafi AM, Richtera L, Adam V (2024) 2D graphene-based advanced nanoarchitectonics for electrochemical biosensors: applications in cancer biomarker detection. Biosens Bioelectron 250:116050. https:\/\/doi.org\/10.1016\/j.bios.2024.116050","journal-title":"Biosens Bioelectron"},{"issue":"8","key":"7451_CR40","doi-asserted-by":"publisher","DOI":"10.3390\/bios12080607","volume":"12","author":"D Ozkan-Ariksoysal","year":"2022","unstructured":"Ozkan-Ariksoysal D (2022) Current perspectives in graphene oxide-based electrochemical biosensors for cancer diagnostics. Biosensors 12(8):607. https:\/\/doi.org\/10.3390\/bios12080607","journal-title":"Biosensors"},{"key":"7451_CR41","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1016\/j.snb.2018.07.055","volume":"275","author":"N Jaiswal","year":"2018","unstructured":"Jaiswal N, Pandey CM, Soni A, Tiwari I, Rosillo-Lopez M, Salzmann CG, Malhotra BD, Sumana G (2018) Electrochemical genosensor based on carboxylated graphene for detection of water-borne pathogen. Sensor Actuats B-Chem 275:312\u2013321. https:\/\/doi.org\/10.1016\/j.snb.2018.07.055","journal-title":"Sensor Actuats B-Chem"},{"key":"7451_CR42","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.talanta.2016.12.042","volume":"165","author":"L Yu","year":"2017","unstructured":"Yu L, Li P, Ding X, Zhang Q (2017) Graphene oxide and carboxylated graphene oxide: viable two-dimensional nanolabels for lateral flow immunoassays. Talanta 165:167\u2013175. https:\/\/doi.org\/10.1016\/j.talanta.2016.12.042","journal-title":"Talanta"},{"issue":"17","key":"7451_CR43","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph191710610","volume":"19","author":"S Farooq","year":"2022","unstructured":"Farooq S, Aziz H, Ali S, Murtaza G, Rizwan M, Saleem MH, Mahboob S, Al-Ghanim KA, Riaz MN, Murtaza B (2022) Synthesis of functionalized carboxylated graphene oxide for the remediation of Pb and Cr contaminated water. Int J Environ Res Public Health 19(17):10610. https:\/\/doi.org\/10.3390\/ijerph191710610","journal-title":"Int J Environ Res Public Health"},{"key":"7451_CR44","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116277","volume":"256","author":"MA Nalepa","year":"2024","unstructured":"Nalepa MA, Pan\u00e1\u010dek D, D\u011bdek I, Jakubec P, Kupka V, Hrub\u00fd V, Petr M, Otyepka M (2024) Graphene derivative-based ink advances inkjet printing technology for fabrication of electrochemical sensors and biosensors. Biosens Bioelectron 256:116277. https:\/\/doi.org\/10.1016\/j.bios.2024.116277","journal-title":"Biosens Bioelectron"},{"issue":"11","key":"7451_CR45","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e21417","volume":"9","author":"D Smr\u017eov\u00e1","year":"2023","unstructured":"Smr\u017eov\u00e1 D, Slu\u0161n\u00e1 M\u0160, Peter J, Bene\u0161 H, Tolasz J, Georgievski OP, Ma\u0159\u00edkov\u00e1 M, Ecorchard P (2023) The effects of formation and functionalization of graphene-based membranes on their gas and water vapor permeation properties. Heliyon 9(11):e21417. https:\/\/doi.org\/10.1016\/j.heliyon.2023.e21417","journal-title":"Heliyon"},{"issue":"2","key":"7451_CR46","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1039\/C5CC08252H","volume":"52","author":"R Xing","year":"2016","unstructured":"Xing R, Li Y, Yu H (2016) Preparation of fluoro-functionalized graphene oxide via the Hunsdiecker reaction. Chem Commun 52(2):390\u2013393. https:\/\/doi.org\/10.1039\/C5CC08252H","journal-title":"Chem Commun"},{"issue":"19","key":"7451_CR47","doi-asserted-by":"publisher","first-page":"2206","DOI":"10.1002\/adma.200903783","volume":"22","author":"Y Song","year":"2010","unstructured":"Song Y, Qu K, Zhao C, Ren J, Qu X (2010) Graphene oxide: intrinsic peroxidase catalytic activity and its application to glucose detection. Adv Mater 22(19):2206\u20132210. https:\/\/doi.org\/10.1002\/adma.200903783","journal-title":"Adv Mater"},{"key":"7451_CR48","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2022.135254","author":"J Suriyaprakash","year":"2022","unstructured":"Suriyaprakash J, Gupta N, Wu L, Shan L (2022) Engineering of all solution\/substrate processable biosensors for the detection of epinephrine as low as pM with rapid readout. Chem Eng J. https:\/\/doi.org\/10.1016\/j.cej.2022.135254","journal-title":"Chem Eng J"},{"key":"7451_CR49","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2023.125561","volume":"270","author":"Y Ding","year":"2024","unstructured":"Ding Y, Zhang Y, Huang C, Wang J, Li H, Wang X (2024) An electrochemical biosensor based on phage-encoded protein RBP 41 for rapid and sensitive detection of Salmonella. Talanta 270:125561. https:\/\/doi.org\/10.1016\/j.talanta.2023.125561","journal-title":"Talanta"},{"issue":"8","key":"7451_CR50","doi-asserted-by":"publisher","DOI":"10.3390\/nano10081446","volume":"10","author":"AI Pruna","year":"2020","unstructured":"Pruna AI, Barjola A, C\u00e1rcel AC, Alonso B, Gim\u00e9nez E (2020) Effect of varying amine functionalities on CO2 capture of carboxylated graphene oxide-based cryogels. Nanomaterials 10(8):1446. https:\/\/doi.org\/10.3390\/nano10081446","journal-title":"Nanomaterials"},{"issue":"1","key":"7451_CR51","doi-asserted-by":"publisher","DOI":"10.3390\/bios14010010","volume":"14","author":"R Wang","year":"2023","unstructured":"Wang R, Zhu B, Young P, Luo Y, Taylor J, Cameron AJ, Squire CJ, Travas-Sejdic J (2023) A portable and disposable electrochemical sensor utilizing laser-scribed graphene for rapid SARS-CoV-2 detection. Biosensors 14(1):10. https:\/\/doi.org\/10.3390\/bios14010010","journal-title":"Biosensors"},{"key":"7451_CR52","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1016\/j.bios.2013.11.053","volume":"54","author":"R Hern\u00e1ndez","year":"2014","unstructured":"Hern\u00e1ndez R, Vall\u00e9s C, Benito AM, Maser WK, Xavier Rius F, Riu J (2014) Graphene-based potentiometric biosensor for the immediate detection of living bacteria. Biosens Bioelectron 54:553\u2013557. https:\/\/doi.org\/10.1016\/j.bios.2013.11.053","journal-title":"Biosens Bioelectron"},{"issue":"7686","key":"7451_CR53","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1038\/nature25021","volume":"553","author":"RW Bourdeau","year":"2018","unstructured":"Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Kumar SR, Nety SP, Shapiro MG (2018) Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature 553(7686):86\u201390. https:\/\/doi.org\/10.1038\/nature25021","journal-title":"Nature"},{"issue":"6","key":"7451_CR54","doi-asserted-by":"publisher","first-page":"2257","DOI":"10.1021\/acsabm.3c00116","volume":"6","author":"D Chauhan","year":"2023","unstructured":"Chauhan D, Chandra R, Kumar S (2023) Hemocompatible functionalized hydrogen substituted graphdiyne based highly durable biosensor for liver cancer detection. ACS Appl Bio Mater 6(6):2257\u20132265. https:\/\/doi.org\/10.1021\/acsabm.3c00116","journal-title":"ACS Appl Bio Mater"},{"issue":"1","key":"7451_CR55","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00604-019-3921-8","volume":"187","author":"N Jaiswal","year":"2019","unstructured":"Jaiswal N, Pandey CM, Solanki S, Tiwari I, Malhotra BD (2019) An impedimetric biosensor based on electrophoretically assembled ZnO nanorods and carboxylated graphene nanoflakes on an indium tin oxide electrode for detection of the DNA of Escherichia coli O157:H7. Microchim Acta 187(1):1. https:\/\/doi.org\/10.1007\/s00604-019-3921-8","journal-title":"Microchim Acta"},{"key":"7451_CR56","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.bios.2015.11.084","volume":"78","author":"S Kumar","year":"2016","unstructured":"Kumar S, Sharma JG, Maji S, Malhotra BD (2016) Nanostructured zirconia decorated reduced graphene oxide based efficient biosensing platform for non-invasive oral cancer detection. Biosens Bioelectron 78:497\u2013504. https:\/\/doi.org\/10.1016\/j.bios.2015.11.084","journal-title":"Biosens Bioelectron"},{"key":"7451_CR57","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2023.143630","volume":"475","author":"M Chaturvedi","year":"2024","unstructured":"Chaturvedi M, Patel M, Mondal DP, Srivastava AK, Dwivedi N, Dhand C (2024) Bio-inspired graphene nanocomposite enabled electrochemical immunosensor for detection and quantification of NS1 protein of dengue virus. Electrochim Acta 475:143630. https:\/\/doi.org\/10.1016\/j.electacta.2023.143630","journal-title":"Electrochim Acta"},{"key":"7451_CR58","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/j.bios.2017.11.004","volume":"102","author":"S Kumar","year":"2018","unstructured":"Kumar S, Ashish KS, Augustine S, Yadav S, Yadav BK, Chauhan RP, Dewan AK, Malhotra BD (2018) Effect of Brownian motion on reduced agglomeration of nanostructured metal oxide towards development of efficient cancer biosensor. Biosens Bioelectron 102:247\u2013255. https:\/\/doi.org\/10.1016\/j.bios.2017.11.004","journal-title":"Biosens Bioelectron"},{"key":"7451_CR59","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.127260","volume":"253","author":"A Kalkal","year":"2023","unstructured":"Kalkal A, Tiwari A, Sharma D, Baghel MK, Kumar P, Pradhan R, Packirisamy G (2023) Air-brush spray coated Ti3C2-MXene-graphene nanohybrid thin film based electrochemical biosensor for cancer biomarker detection. Int J Biol Macromol 253:127260. https:\/\/doi.org\/10.1016\/j.ijbiomac.2023.127260","journal-title":"Int J Biol Macromol"},{"issue":"5","key":"7451_CR60","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1177\/2211068215586977","volume":"21","author":"Y-S Sun","year":"2016","unstructured":"Sun Y-S, Zhu X (2016) Characterization of bovine serum albumin blocking efficiency on epoxy-functionalized substrates for microarray applications. SLAS Technol 21(5):625\u2013631. https:\/\/doi.org\/10.1177\/2211068215586977","journal-title":"SLAS Technol"},{"issue":"38","key":"7451_CR61","doi-asserted-by":"publisher","first-page":"32047","DOI":"10.1021\/acsami.8b13749","volume":"10","author":"JH Park","year":"2018","unstructured":"Park JH, Jackman JA, Ferhan AR, Ma GJ, Yoon BK, Cho N-J (2018) Temperature-induced denaturation of BSA protein molecules for improved surface passivation coatings. ACS Appl Mater Interfaces 10(38):32047\u201332057. https:\/\/doi.org\/10.1021\/acsami.8b13749","journal-title":"ACS Appl Mater Interfaces"},{"key":"7451_CR62","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2020.113598","volume":"593","author":"Y Cui","year":"2020","unstructured":"Cui Y (2020) Optimization of blocking conditions for fluorescent Western blot. Anal Biochem 593:113598. https:\/\/doi.org\/10.1016\/j.ab.2020.113598","journal-title":"Anal Biochem"},{"issue":"2","key":"7451_CR63","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/S0165-0270(97)00126-X","volume":"77","author":"P Tagliaferro","year":"1997","unstructured":"Tagliaferro P, Tandler CJ, Ramos AJ, Pecci Saavedra J, Brusco A (1997) Immunofluorescence and glutaraldehyde fixation. A new procedure based on the Schiff-quenching method. J Neurosci Methods 77(2):191\u2013197. https:\/\/doi.org\/10.1016\/S0165-0270(97)00126-X","journal-title":"J Neurosci Methods"},{"issue":"1","key":"7451_CR64","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.ab.2012.03.028","volume":"426","author":"C Galva","year":"2012","unstructured":"Galva C, Gatto C, Milanick M (2012) Soymilk: an effective and inexpensive blocking agent for immunoblotting. Anal Biochem 426(1):22\u201323. https:\/\/doi.org\/10.1016\/j.ab.2012.03.028","journal-title":"Anal Biochem"},{"key":"7451_CR65","unstructured":"Parker NSM, Tu A-H T, Lister P, Forster BM (2016) Microbiology, detecting antigen-antibody complexes. https:\/\/openstax.org\/details\/books\/microbiology. Accessed 1 Nov 2016"},{"key":"7451_CR66","unstructured":"WHO (2022) Guidelines for drinking-water quality: fourth edition incorporating the first and second addenda. Licence: CC BY-NC-SA 3.0 IGO. https:\/\/www.who.int\/publications\/i\/item\/9789240045064. Accessed 21 Mar2022"},{"key":"7451_CR67","unstructured":"State Environmental Protection Administration, General Administration of Quality Supervision, Inspection and Quarantine (2002) Surface water environmental quality standard: GB 3838\u20132002. China Environ Sci Press. https:\/\/english.mee.gov.cn\/Resources\/standards\/water_environment\/quality_standard\/200710\/t20071024_111792.shtml. Accessed 1 Jun 2002"},{"issue":"1","key":"7451_CR68","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/s0167-7012(03)00133-7","volume":"55","author":"TB Tims","year":"2003","unstructured":"Tims TB, Lim DV (2003) Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection. J Microbiol Methods 55(1):141\u2013147. https:\/\/doi.org\/10.1016\/s0167-7012(03)00133-7","journal-title":"J Microbiol Methods"},{"issue":"6","key":"7451_CR69","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1016\/j.bios.2005.02.012","volume":"21","author":"JM Simpson","year":"2005","unstructured":"Simpson JM, Lim DV (2005) Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection. Biosens Bioelectron 21(6):881\u2013887. https:\/\/doi.org\/10.1016\/j.bios.2005.02.012","journal-title":"Biosens Bioelectron"},{"key":"7451_CR70","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2019.111415","volume":"141","author":"YM Park","year":"2019","unstructured":"Park YM, Kim CH, Lee SJ, Lee M-K (2019) Multifunctional hand-held sensor using electronic components embedded in smartphones for quick PCR screening. Biosens Bioelectron 141:111415. https:\/\/doi.org\/10.1016\/j.bios.2019.111415","journal-title":"Biosens Bioelectron"},{"issue":"8","key":"7451_CR71","doi-asserted-by":"publisher","first-page":"3106","DOI":"10.1039\/d0an00224k","volume":"145","author":"Y Zhao","year":"2020","unstructured":"Zhao Y, Zeng D, Yan C, Chen W, Ren J, Jiang Y, Jiang L, Xue F, Ji D, Tang F, Zhou M, Dai J (2020) Rapid and accurate detection of Escherichia coli O157:H7 in beef using microfluidic wax-printed paper-based ELISA. Analyst 145(8):3106\u20133115. https:\/\/doi.org\/10.1039\/d0an00224k","journal-title":"Analyst"},{"key":"7451_CR72","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1016\/j.bios.2018.06.005","volume":"117","author":"C Zhou","year":"2018","unstructured":"Zhou C, Zou H, Li M, Sun C, Ren D, Li Y (2018) Fiber optic surface plasmon resonance sensor for detection of E. coli O157:H7 based on antimicrobial peptides and AgNPs-rGO. Biosens Bioelectron 117:347\u2013353. https:\/\/doi.org\/10.1016\/j.bios.2018.06.005","journal-title":"Biosens Bioelectron"},{"issue":"2","key":"7451_CR73","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1021\/acssensors.9b02600","volume":"5","author":"S Zhou","year":"2020","unstructured":"Zhou S, Lu C, Li Y, Xue L, Zhao C, Tian G, Bao Y, Tang L, Lin J, Zheng J (2020) Gold nanobones enhanced ultrasensitive surface-enhanced raman scattering aptasensor for detecting Escherichia coli O157:H7. ACS Sens 5(2):588\u2013596. https:\/\/doi.org\/10.1021\/acssensors.9b02600","journal-title":"ACS Sens"},{"key":"7451_CR74","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.snb.2018.03.014","volume":"265","author":"L Xue","year":"2018","unstructured":"Xue L, Zheng L, Zhang H, Jin X, Lin J (2018) An ultrasensitive fluorescent biosensor using high gradient magnetic separation and quantum dots for fast detection of foodborne pathogenic bacteria. Sensor Actuats B-Chem 265:318\u2013325. https:\/\/doi.org\/10.1016\/j.snb.2018.03.014","journal-title":"Sensor Actuats B-Chem"},{"issue":"8","key":"7451_CR75","doi-asserted-by":"publisher","DOI":"10.3390\/s20082267","volume":"20","author":"Y Jo","year":"2020","unstructured":"Jo Y, Park J, Park J-K (2020) Colorimetric detection of Escherichia coli O157:H7 with signal enhancement using size-based filtration on a finger-powered microfluidic device. Sensors (Basel) 20(8):2267. https:\/\/doi.org\/10.3390\/s20082267","journal-title":"Sensors (Basel)"},{"key":"7451_CR76","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2023.341810","author":"M Du","year":"2023","unstructured":"Du M, Ma J, Zhang Z, Wu G, Wu J, Wang H, Xie X, Wang C (2023) Direct, ultrafast, and sensitive detection of environmental pathogenic microorganisms based on a graphene biosensor. Anal Chim Acta. https:\/\/doi.org\/10.1016\/j.aca.2023.341810","journal-title":"Anal Chim Acta"},{"issue":"6","key":"7451_CR77","doi-asserted-by":"publisher","first-page":"5430","DOI":"10.1021\/acsami.6b12455","volume":"9","author":"S Chen","year":"2017","unstructured":"Chen S, Chen X, Zhang L, Gao J, Ma Q (2017) Electrochemiluminescence detection of Escherichia coli O157:H7 based on a novel polydopamine surface imprinted polymer biosensor. ACS Appl Mater Interfaces 9(6):5430\u20135436. https:\/\/doi.org\/10.1021\/acsami.6b12455","journal-title":"ACS Appl Mater Interfaces"},{"key":"7451_CR78","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1016\/j.snb.2017.12.110","volume":"259","author":"L Yao","year":"2018","unstructured":"Yao L, Wang L, Huang F, Cai G, Xi X, Lin J (2018) A microfluidic impedance biosensor based on immunomagnetic separation and urease catalysis for continuous-flow detection of E. coli O157:H7. Sensor Actuats B-Chem 259:1013\u20131021. https:\/\/doi.org\/10.1016\/j.snb.2017.12.110","journal-title":"Sensor Actuats B-Chem"}],"container-title":["Microchimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-025-07451-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00604-025-07451-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-025-07451-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T10:31:50Z","timestamp":1757413910000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00604-025-07451-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,19]]},"references-count":78,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["7451"],"URL":"https:\/\/doi.org\/10.1007\/s00604-025-07451-z","relation":{},"ISSN":["0026-3672","1436-5073"],"issn-type":[{"value":"0026-3672","type":"print"},{"value":"1436-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,19]]},"assertion":[{"value":"2 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 August 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"600"}}