{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T08:07:42Z","timestamp":1769155662950,"version":"3.49.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T00:00:00Z","timestamp":1766188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T00:00:00Z","timestamp":1766188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"crossref","award":["No. ZR2023QB060"],"award-info":[{"award-number":["No. ZR2023QB060"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Foundation for Young Scientists of China","award":["No. 22406064"],"award-info":[{"award-number":["No. 22406064"]}]},{"name":"Shandong Provincial Young Science and Technology Talents Support Project","award":["SDAST2025 QTB023"],"award-info":[{"award-number":["SDAST2025 QTB023"]}]},{"name":"National Science Fund for China Postdoctoral Science Foundation","award":["No. 2023M731304"],"award-info":[{"award-number":["No. 2023M731304"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microchim Acta"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1007\/s00604-025-07730-9","type":"journal-article","created":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T03:41:40Z","timestamp":1766202100000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A sensitive chemiluminescence aptasensor for adenosine detection based on a nucleic acid cycle amplification strategy"],"prefix":"10.1007","volume":"193","author":[{"given":"Yuanling","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"jiajia","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuannan","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,20]]},"reference":[{"key":"7730_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fimmu.2014.00304","volume":"5","author":"A Ohta","year":"2014","unstructured":"Ohta A, Sitkovsky M (2014) Extracellular adenosine-mediated modulation of regulatory T cells. Front Immunol 5:1\u20139","journal-title":"Front Immunol"},{"issue":"3","key":"7730_CR2","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1038\/onc.2016.206","volume":"36","author":"F Di Virgilio","year":"2017","unstructured":"Di Virgilio F, Adinolfi E (2017) Extracellular purines, purinergic receptors and tumor growth. Oncogene 36(3):293\u2013303","journal-title":"Oncogene"},{"key":"7730_CR3","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/0076-6879(81)74025-4","volume":"74","author":"PE Daddona","year":"1981","unstructured":"Daddona PE, Frohman MA, Kelley WN (1981) Radioimmunoassay of human adenosine deaminase. Methods Enzymol 74:351\u2013358","journal-title":"Methods Enzymol"},{"issue":"1","key":"7730_CR4","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/S0003-2697(02)00535-3","volume":"313","author":"M Ushimaru","year":"2003","unstructured":"Ushimaru M, Fukushima Y (2003) Complete separation of adenine nucleotides for ATPase activity assay by ion-pair reversed-phase high-performance liquid chromatography. Anal Biochem 313(1):173\u2013175","journal-title":"Anal Biochem"},{"issue":"9","key":"7730_CR5","doi-asserted-by":"publisher","first-page":"6764","DOI":"10.1021\/acs.analchem.0c00996","volume":"92","author":"M Jender","year":"2020","unstructured":"Jender M, Novo P, Maehler D, Miinchberg U, Janasek D, Freier E (2020) Multiplexed online monitoring of microfluidic free-flow electrophoresis via mass spectrometry. Anal Chem 92(9):6764\u20136769","journal-title":"Anal Chem"},{"issue":"12","key":"7730_CR6","doi-asserted-by":"publisher","first-page":"6674","DOI":"10.1021\/acsapm.1c01348","volume":"3","author":"DO Runsewe","year":"2021","unstructured":"Runsewe DO, Haya G, Betancourt T, Irvin JA (2021) Conducting polymer-based electrochemical aptasensor for the detection of adenosine. ACS Appl Polym Mater 3(12):6674\u20136683","journal-title":"ACS Appl Polym Mater"},{"key":"7730_CR7","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1016\/j.bios.2016.06.089","volume":"86","author":"YX Liu","year":"2016","unstructured":"Liu YX, Ma HM, Zhang Y, Pang XH, Fan DW, Wu D, Wei Q (2016) Visible light photoelectrochemical aptasensor for adenosine detection based on CdS\/PPy\/g-C<Subscript>3<\/Subscript>N<Subscript>4<\/Subscript> nanocomposites. Biosensors & Bioelectronics 86:439\u2013445","journal-title":"Biosensors & Bioelectronics"},{"key":"7730_CR8","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1016\/j.electacta.2012.05.056","volume":"76","author":"HY Wang","year":"2012","unstructured":"Wang HY, Gong W, Tan ZA, Yin XX, Wang L (2012) Label-free bifunctional electrochemiluminescence aptasensor for detection of adenosine and lysozyme. Electrochim Acta 76:416\u2013423","journal-title":"Electrochim Acta"},{"issue":"12","key":"7730_CR9","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.2116\/analsci.27.1185","volume":"27","author":"YQ Jin","year":"2012","unstructured":"Jin YQ, Shi WJ, Zhou MY, Tu YF, Yan JL (2012) Simple chemiluminescence aptasensors based on resonance energy transfer. Anal Sci 27(12):1185\u20131190","journal-title":"Anal Sci"},{"key":"7730_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2023.109392","volume":"195","author":"PG Cheng","year":"2023","unstructured":"Cheng PG, Guo WJ, Li RQ, Yang YL, Du QZ (2023) Dual recognition ratio fluorescence-based sensor for sensitive detection of adenosine. \u200c. Microchem J 195:109392","journal-title":"Microchem J"},{"issue":"4","key":"7730_CR11","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1134\/S1061934825700017","volume":"80","author":"M Asghar","year":"2025","unstructured":"Asghar M, Yaqoob M, Siddiqui MA, Waseem A (2025) Analytical applications of chemiluminescence in flow-injection systems. J Anal Chem 80(4):565\u2013590","journal-title":"J Anal Chem"},{"issue":"6","key":"7730_CR12","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1134\/S1061934812040028","volume":"67","author":"YB Tsaplev","year":"2012","unstructured":"Tsaplev YB (2012) Chemiluminescence determination of hydrogen peroxide. J Anal Chem 67(6):506\u2013514","journal-title":"J Anal Chem"},{"key":"7730_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2023.117213","volume":"166","author":"LX Zhao","year":"2023","unstructured":"Zhao LX, Xu J, Xiong L, Wang SL, Yu CZ, Lv JT, Lin JM (2023) Recent development of chemiluminescence for bioanalysis. TrAC Trends Anal Chem 166:117213","journal-title":"TrAC Trends Anal Chem"},{"issue":"1","key":"7730_CR14","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1385\/MB:19:1:029","volume":"19","author":"SC Andras","year":"2001","unstructured":"Andras SC, Power JB, Cocking EC, Davey MR (2001) Strategies for signal amplification in nucleic acid detection. Mol Biotechnol 19(1):29\u201344","journal-title":"Mol Biotechnol"},{"key":"7730_CR15","doi-asserted-by":"publisher","first-page":"1019071","DOI":"10.3389\/fcimb.2022.1019071","volume":"12","author":"MY Tan","year":"2022","unstructured":"Tan MY, Liao C, Liang LA, Yi XL, Zhou ZH, Wei GJ (2022) Recent advances in recombinase polymerase amplification: principle, advantages, disadvantages and applications. Front Cell Infect Microbiol 12:1019071","journal-title":"Front Cell Infect Microbiol"},{"key":"7730_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2021.122637","volume":"234","author":"JT Wu","year":"2021","unstructured":"Wu JT, Lv JR, Zheng XQ, Wu ZS (2021) Hybridization chain reaction and its applications in biosensing. Talanta 234:122637","journal-title":"Talanta"},{"issue":"12","key":"7730_CR17","doi-asserted-by":"publisher","first-page":"7474","DOI":"10.1021\/acs.analchem.8b01015","volume":"90","author":"Ultrasensitive Fluorescent Assay Based on a Rolling-Circle","year":"2018","unstructured":"Ultrasensitive Fluorescent Assay Based on a Rolling-Circle- (2018) Amplification- assisted Multisite-Strand-Displacement-Reaction Signal-Amplification strategy. Anal Chem 90(12):7474\u20137479","journal-title":"Anal Chem"},{"issue":"22","key":"7730_CR18","doi-asserted-by":"publisher","first-page":"6142","DOI":"10.1002\/anie.201700054","volume":"56","author":"M Liu","year":"2017","unstructured":"Liu M, Zhang Q, Chang DR, Gu J, Brennan JD, Li YF (2017) A DNAzyme feedback amplification strategy for biosensing. Angew Chem Int Ed 56(22):6142\u20136146","journal-title":"Angew Chem Int Ed"},{"key":"7730_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2022.339663","volume":"1204","author":"WW Liu","year":"2022","unstructured":"Liu WW, Zhang XL, Zhu L, Xu S, Chai YQ, Li ZH, Yuan R (2022) Mismatch-fueled catalytic hairpin assembly mediated ultrasensitive biosensor for rapid detection of MicroRNA. Anal Chim Acta 1204:339663","journal-title":"Anal Chim Acta"},{"key":"7730_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.bios.2018.04.046","volume":"113","author":"ZQ Chen","year":"2018","unstructured":"Chen ZQ, Liu Y, Xin C, Zhao JK, Liu SF (2018) A cascade autocatalytic strand displacement amplification and hybridization chain reaction event for label-free and ultrasensitive electrochemical nucleic acid biosensing. \u200c. Biosens Bioelectron 113:1\u20138","journal-title":"Biosens Bioelectron"},{"key":"7730_CR21","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/j.snb.2017.10.188","volume":"257","author":"H Wang","year":"2018","unstructured":"Wang H, Jian YN, Kong QK, Liu HY, Lan FF, Liang LL, Ge SG, Yu JH (2018) Ultrasensitive electrochemical paper-based biosensor for MicroRNA via strand displacement reaction and metal-organic frameworks. Sens Actuators B 257:561\u2013569","journal-title":"Sens Actuators B"},{"issue":"4","key":"7730_CR22","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1007\/s11814-021-0755-y","volume":"38","author":"JH Yang","year":"2021","unstructured":"Yang JH (2021) Hydrogen sulfide removal technology: a focused review on adsorption and catalytic oxidation. Korean J Chem Eng 38(4):674\u2013691","journal-title":"Korean J Chem Eng"},{"key":"7730_CR23","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.catcom.2018.06.023","volume":"115","author":"BJ Gao","year":"2018","unstructured":"Gao BJ, Bi CC (2018) Some catalytic characteristics of compositional catalysts of immobilized N-hydroxyphthalimide and metal salts in aerobic oxidation of 1-phenylethanol. Catal Commun 115:6\u201311","journal-title":"Catal Commun"},{"key":"7730_CR24","doi-asserted-by":"crossref","unstructured":"Han XX, Ji W, Zhao B, Ozaki Y (2017) Semiconductor-enhanced Raman scattering: active nanomaterials and applications. Nanoscale, 9(15): 4847\u20134861. Nanoscale, 2017, 9(15): 4847\u20134861","DOI":"10.1039\/C6NR08693D"},{"issue":"4","key":"7730_CR25","doi-asserted-by":"publisher","first-page":"2533","DOI":"10.1021\/acsnano.3c07680","volume":"18","author":"HZ Fan","year":"2024","unstructured":"Fan HZ, Zhang RF, Fan KL, Gao LZ, Yan XY (2024) Exploring the specificity of nanozymes. ACS Nano 18(4):2533\u20132540","journal-title":"ACS Nano"},{"issue":"17","key":"7730_CR26","doi-asserted-by":"publisher","first-page":"20985","DOI":"10.1021\/acsanm.4c03947","volume":"7","author":"B Li","year":"2024","unstructured":"Li B, Liu H, Xing XR, Zeng CX, Zhang M, Li HL, Lu N (2024) Carbon-decorated Fe3O4 nanocomposites as enhanced enzyme mimics for colorimetric determination of H2O2 and glucose. ACS Appl Nano Mater 7(17):20985\u201320993","journal-title":"ACS Appl Nano Mater"},{"issue":"91","key":"7730_CR27","doi-asserted-by":"publisher","first-page":"12306","DOI":"10.1039\/C7CC07149C","volume":"53","author":"S Mumtaz","year":"2017","unstructured":"Mumtaz S, Wang S, Hussain SZ, Abdullah M, Huma Z, Iqbal Z, Creran B, Rotello VM, Hussain I (2017) Dopamine coated Fe3O4 nanoparticles as enzyme mimics for the sensitive detection of bacteria. Chem Commun 53(91):12306\u201312308","journal-title":"Chem Commun"},{"issue":"5","key":"7730_CR28","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2022.107820","volume":"34","author":"JH Ju","year":"2023","unstructured":"Ju JH, Chen YT, Liu ZQ, Huang C, Li YQ, Kong DZ, Shen W, Tang S (2023) Modification and application of Fe3O4 nanozymes in analytical chemistry: a review. Chin Chem Lett 34(5):107820","journal-title":"Chin Chem Lett"},{"issue":"9","key":"7730_CR29","doi-asserted-by":"publisher","first-page":"1713","DOI":"10.1007\/s00216-024-05679-x","volume":"417","author":"NN Guo","year":"2025","unstructured":"Guo NN, Yang J, Li YX, Wang WI, Liang XW, Xu Q, Du LN, Qin J (2025) A review of a colorimetric biosensor based on Fe3O4 nanozymes for food safety detection. Anal Bioanal Chem 417(9):1713\u20131730","journal-title":"Anal Bioanal Chem"},{"key":"7730_CR30","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.apsusc.2018.01.152","volume":"440","author":"SH Chen","year":"2018","unstructured":"Chen SH, Chi MQ, Zhu Y, Gao M, Wang C, Lu XF (2018) A facile synthesis of superparamagnetic Fe3O4 nanofibers with superior peroxidase-like catalytic activity for sensitive colorimetric detection of L-cysteine. Appl Surf Sci 440:237\u2013244","journal-title":"Appl Surf Sci"},{"issue":"3","key":"7730_CR31","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1039\/C3GC41535J","volume":"16","author":"Y Gao","year":"2014","unstructured":"Gao Y, Wei Z, Li F, Yang ZM, Chen YM, Zrinyi M, Osada Y (2014) Synthesis of a morphology controllable Fe3O4 nanoparticle\/hydrogel magnetic nanocomposite inspired by magnetotactic bacteria and its application in H2O2 detection. Green Chem 16(3):1255\u20131261","journal-title":"Green Chem"},{"issue":"34","key":"7730_CR32","doi-asserted-by":"publisher","first-page":"5652","DOI":"10.1039\/C4TB00705K","volume":"2","author":"H Li","year":"2014","unstructured":"Li H, Kong WQ, Liu J, Yang MM, Huang H, Liu Y, Kang ZH (2014) Carbon dots for photoswitching enzyme catalytic activity. J Mater Chem B 2(34):5652\u20135658","journal-title":"J Mater Chem B"},{"key":"7730_CR33","first-page":"712A","volume":"55","author":"GL Long","year":"1983","unstructured":"Long GL, Winefordner JD (1983) Limit of detection a closer look at the IUPAC definition. Anal Chem 55:712A\u2013724A","journal-title":"Anal Chem"},{"key":"7730_CR34","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1021\/ja053121d","volume":"128","author":"AE Radi","year":"2006","unstructured":"Radi AE, Acero Sanchez JL, Baldrich \u00b4E, Sullivan CKO\u2019 (2006) Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor. J Am Chem Soc 128:117\u2013124","journal-title":"J Am Chem Soc"},{"key":"7730_CR35","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2023.341212","volume":"1260","author":"SD Cao","year":"2023","unstructured":"Cao SD, Zhao HL, Chen KC, Zhou FF, Lan MB (2023) An electrochemical aptasensor based on multi-walled carbon nanotubes loaded with PtCu nanoparticles as signal label for ultrasensitive detection of adenosine. Anal Chim Acta 1260:341212","journal-title":"Anal Chim Acta"},{"key":"7730_CR36","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2019.111741","volume":"146","author":"Y Gao","year":"2019","unstructured":"Gao Y, Qi H, Shang MX, Zhang JL, Yan JY, Song WB (2019) Carbon dots-sensitized amorphous MoSx photoanode: sequential electrodeposition preparation and dual amplified photoelectrochemical aptasensing of adenosine. Biosens Bioelectron 146:111741","journal-title":"Biosens Bioelectron"},{"issue":"9","key":"7730_CR37","first-page":"1353","volume":"45","author":"XM Fan","year":"2017","unstructured":"Fan XM, Wang SM, Li ZJ, Jia XY, Zheng XW (2017) Determination of adenosine based on Ru(bpy)32+\/AuNPs\/SWCNTs\/Adenosine aptamer electrochemiluminescence sensor. Chin J Anal Chem 45(9):1353\u20131359","journal-title":"Chin J Anal Chem"},{"key":"7730_CR38","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.talanta.2018.04.068","volume":"186","author":"YL Sun","year":"2018","unstructured":"Sun YL, Ding CF, Lin YN, Sun WY, Liu H, Zhu XD, Dai YX, Luo CN (2018) Highly selective and sensitive chemiluminescence biosensor for adenosine detection based on carbon quantum dots catalyzing luminescence released from aptamers functionalized graphene@magnetic \u03b2-cyclodextrin polymers. Talanta 186:238\u2013247","journal-title":"Talanta"},{"key":"7730_CR39","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2021.339386","volume":"1195","author":"YN Hou","year":"2022","unstructured":"Hou YN, Han R, Sun YL, Luo CN, Wang XY (2022) Chemiluminescence sensing of adenosine using DNA cross-linked hydrogel-capped magnetic mesoporous silica nanoparticles. Anal Chim Acta 1195:339386","journal-title":"Anal Chim Acta"},{"key":"7730_CR40","doi-asserted-by":"crossref","unstructured":"Zhang X, Kong CY, Liu QY, Zuo X, Li K, Chen ZB Colorimetric adenosine assay based on the self-assembly of aptamer-functionalized gold nanorods. Microchim Acta, 20149, 186(8): 587","DOI":"10.1007\/s00604-019-3680-6"},{"key":"7730_CR41","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2021.129983","volume":"422","author":"Y Li","year":"2021","unstructured":"Li Y, Xin XL, Zhang TT, Li WH, Li JS, Lu R (2021) Raspberry-like polyamide@Ag hybrid nanoarrays with flexible cores and SERS signal enhancement strategy for adenosine detection. Chem Eng J 422:129983","journal-title":"Chem Eng J"},{"key":"7730_CR42","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2023.123557","volume":"305","author":"P Li","year":"2023","unstructured":"Li P, Luo C, Chen XX, Huang CB (2023) A novel \u201coff-on\u201d ratiometric fluorescent aptasensor for adenosine detection based on FRET between quantum dots and graphene oxide. Spectrochim Acta A Mol Biomol Spectrosc 305:123557","journal-title":"Spectrochim Acta A Mol Biomol Spectrosc"}],"container-title":["Microchimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-025-07730-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00604-025-07730-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-025-07730-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T16:57:41Z","timestamp":1769101061000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00604-025-07730-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,20]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["7730"],"URL":"https:\/\/doi.org\/10.1007\/s00604-025-07730-9","relation":{},"ISSN":["0026-3672","1436-5073"],"issn-type":[{"value":"0026-3672","type":"print"},{"value":"1436-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,20]]},"assertion":[{"value":"26 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Reagents and apparatus, calculation of LOD, Fig.\n                      \n                      -S4, and other supplementary results are available in Supporting Information.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Supporting information"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"36"}}