{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:51:46Z","timestamp":1792068706371,"version":"4.4.0"},"reference-count":71,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["JDC2022-048757-I"],"award-info":[{"award-number":["JDC2022-048757-I"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PREP2022-000170"],"award-info":[{"award-number":["PREP2022-000170"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2022-141293OB-I00"],"award-info":[{"award-number":["PID2022-141293OB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2022-140307OB-I00"],"award-info":[{"award-number":["PID2022-140307OB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2022-136351OB-I00"],"award-info":[{"award-number":["PID2022-136351OB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AGAUR\/Generalitat de Catalunya","award":["2021 SGR 00052"],"award-info":[{"award-number":["2021 SGR 00052"]}]},{"name":"AES-ISCIII","award":["PI20CIII\/00019"],"award-info":[{"award-number":["PI20CIII\/00019"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microchim Acta"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1007\/s00604-026-08049-9","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T09:23:33Z","timestamp":1776677013000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["All-in-one silk-fibroin sustainable high performance electrochemical immunoplatforms: determination of the protein TIM-1 in cancer and allergy scenarios as a case study"],"prefix":"10.1007","volume":"193","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1206-1455","authenticated-orcid":false,"given":"Sara","family":"Santiago","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0785-1495","authenticated-orcid":false,"given":"Andrea","family":"Cabrero-Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0034-8456","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Seraf\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2047-396X","authenticated-orcid":false,"given":"Mar\u00eda","family":"Pedrero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6447-5756","authenticated-orcid":false,"given":"Xavier","family":"Mu\u00f1oz-Berbel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2128-7007","authenticated-orcid":false,"given":"Gonzalo","family":"Guirado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5141-0454","authenticated-orcid":false,"given":"Ana","family":"Montero-Calle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2271-1383","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Pingarr\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3539-7469","authenticated-orcid":false,"given":"Rodrigo","family":"Barderas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9928-6613","authenticated-orcid":false,"given":"Susana","family":"Campuzano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"8049_CR1","unstructured":"Arango N\u00fa\u00f1ez M (2015) Dise\u00f1o de un biomaterial basado en fibro\u00edna para aplicaciones en dispositivos electr\u00f3nicos. Universidad de los Andes (Colombia). https:\/\/hdl.handle.net\/1992\/62194. Accessed Sept 25"},{"key":"8049_CR2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113204","volume":"183","author":"D Molinnus","year":"2021","unstructured":"Molinnus D, Drinic A, Iken H, Kr\u00f6ger N, Zinser M, Smeets R, K\u00f6pf M, Kopp A, Sch\u00f6ning M (2021) Towards a flexible electrochemical biosensor fabricated from biocompatible Bombyx mori silk. Biosens Bioelectron 183:113204. https:\/\/doi.org\/10.1016\/j.bios.2021.113204","journal-title":"Biosens Bioelectron"},{"key":"8049_CR3","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.msec.2018.01.007","volume":"86","author":"LD Koh","year":"2018","unstructured":"Koh LD, Yeo J, Lee YY, Ong Q, Han M, Tee BCK (2018) Advancing the frontiers of silk fibroin protein-based materials for futuristic electronics and clinical wound-healing. Mater Sci Eng C Mater Biol 86:151. https:\/\/doi.org\/10.1016\/j.msec.2018.01.007","journal-title":"Mater Sci Eng C Mater Biol"},{"key":"8049_CR4","doi-asserted-by":"publisher","first-page":"2015","DOI":"10.1021\/acsbiomaterials.1c00080","volume":"7","author":"NJ Prakash","year":"2021","unstructured":"Prakash NJ, Mane PP, George SM, Kandasubramanian B (2021) Silk fibroin as an immobilization matrix for sensing applications. ACS Biomater Sci Eng 7:2015. https:\/\/doi.org\/10.1021\/acsbiomaterials.1c00080","journal-title":"ACS Biomater Sci Eng"},{"key":"8049_CR5","doi-asserted-by":"publisher","first-page":"4337","DOI":"10.1021\/acsbiomaterials.9b01971","volume":"6","author":"S Kaushik","year":"2020","unstructured":"Kaushik S, Thungon PD, Goswami P (2020) Silk fibroin: an emerging biocompatible material for application of enzymes and whole cells in bioelectronics and bioanalytical sciences. ACS Biomater Sci Eng 6:4337. https:\/\/doi.org\/10.1021\/acsbiomaterials.9b01971","journal-title":"ACS Biomater Sci Eng"},{"key":"8049_CR6","doi-asserted-by":"publisher","first-page":"237","DOI":"10.3390\/ijms18030237","volume":"18","author":"Y Qi","year":"2017","unstructured":"Qi Y, Wang H, Wei K, Yang Y, Zheng RY, Kim IS, Zhang KQ (2017) A review of structure construction of silk fibroin biomaterials from single structures to multi-level structures. Int J Mol Sci 18:237. https:\/\/doi.org\/10.3390\/ijms18030237","journal-title":"Int J Mol Sci"},{"key":"8049_CR7","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202210764","volume":"33","author":"A Reizabal","year":"2023","unstructured":"Reizabal A, Costa CM, P\u00e9rez-\u00c1lvarez L, Vilas-Vilela JL, Lanceros-M\u00e9ndez S (2023) Silk fibroin as sustainable advanced material: material properties and characteristics, processing, and applications. Adv Funct Mater 33:2210764. https:\/\/doi.org\/10.1002\/adfm.202210764","journal-title":"Adv Funct Mater"},{"key":"8049_CR8","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.1021\/bm800956n","volume":"10","author":"S Lu","year":"2009","unstructured":"Lu S, Wang X, Lu Q, Hu X, Uppal N, Omenetto FG, Kaplan KLH (2009) Stabilization of enzymes in silk films. Biomacromolecules 10:1032. https:\/\/doi.org\/10.1021\/bm800956n","journal-title":"Biomacromolecules"},{"key":"8049_CR9","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1021\/acsabm.3c00872","volume":"7","author":"A M\u00e1rquez","year":"2024","unstructured":"M\u00e1rquez A, Santiago S, Dos Santos MV, Aznar-Cervantes SD, Dom\u00ednguez C, Omenetto FG, Guirado G, Mu\u00f1oz-Berbel X (2024) Reusable colorimetric biosensors on sustainable silk-based platforms. ACS Appl Bio Mater 7:853. https:\/\/doi.org\/10.1021\/acsabm.3c00872","journal-title":"ACS Appl Bio Mater"},{"key":"8049_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2023.115198","volume":"228","author":"G Meng","year":"2023","unstructured":"Meng G, Long F, Zeng Z, Kong L, Zhao B, Yan J, Yang L, Yang Y, Liu X, Yan Z, Lin N (2023) Silk fibroin based wearable electrochemical sensors with biomimetic enzyme-like activity constructed for durable and on-site health monitoring. Biosens Bioelectron 228:115198. https:\/\/doi.org\/10.1016\/j.bios.2023.115198","journal-title":"Biosens Bioelectron"},{"key":"8049_CR11","doi-asserted-by":"publisher","first-page":"3928","DOI":"10.1021\/acs.biomac.2c00753","volume":"23","author":"Q Chen","year":"2022","unstructured":"Chen Q, Liu Y, Gu K, Yao J, Shao Z, Chen X (2022) Silk-based electrochemical sensor for the detection of glucose in sweat. Biomacromolecules 23:3928. https:\/\/doi.org\/10.1021\/acs.biomac.2c00753","journal-title":"Biomacromolecules"},{"key":"8049_CR12","doi-asserted-by":"publisher","first-page":"29319","DOI":"10.1039\/d2ra05585f","volume":"12","author":"A Noor","year":"2022","unstructured":"Noor A, Khalid H, Aslam HM, Hayat A, Khan AF, Nasir M, Chaudhry AA, Nawaz MH (2022) Graphene oxide reinforced silk fibroin nanocomposite as an electroactive interface for the estimation of dopamine. RSC Adv 12:29319. https:\/\/doi.org\/10.1039\/d2ra05585f","journal-title":"RSC Adv"},{"key":"8049_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2023.108760","volume":"191","author":"D Kuang","year":"2023","unstructured":"Kuang D, Yu W, Liu J, Yin Y, Wang C (2023) Flexible MWCNT\/silk fibroin film decorated with Pt NPs for electrochemical glucose sensors. Microchem J 191:108760. https:\/\/doi.org\/10.1016\/j.microc.2023.108760","journal-title":"Microchem J"},{"key":"8049_CR14","doi-asserted-by":"publisher","DOI":"10.3390\/su152216053","volume":"15","author":"Q Wen","year":"2023","unstructured":"Wen Q, Zhang L, Chen Y, Su Y, Yu J, Chen P, Zheng T (2023) Novel applications of silk proteins based on their interactions with metal ions. Sustainability 15:16053. https:\/\/doi.org\/10.3390\/su152216053","journal-title":"Sustainability"},{"key":"8049_CR15","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1007\/s002160050157","volume":"357","author":"H Liu","year":"1997","unstructured":"Liu H, Qian J, Liu Y, Yu T, Deng J, Qi D (1997) Amperometric methylene blue-mediated sensor highly sensitive to hydrogen peroxide based on a composite membrane of regenerated silk fibroin and poly-vinyl alcohol as immobilization matrix for horseradish peroxidase. Anal Chem 357:302. https:\/\/doi.org\/10.1007\/s002160050157","journal-title":"Anal Chem"},{"key":"8049_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.coco.2024.102122","volume":"52","author":"S Du","year":"2024","unstructured":"Du S, Tan Y, Chen J, Wei Y, Qu Z, Li J, Zhang J, Zhou W (2024) Robust and washable silk fiber-based electrochemical biosensor for high-performance sensing of hydrogen peroxide. Compos Commun 52:102122. https:\/\/doi.org\/10.1016\/j.coco.2024.102122","journal-title":"Compos Commun"},{"key":"8049_CR17","doi-asserted-by":"publisher","first-page":"265","DOI":"10.4028\/www.scientific.net\/AMR.55-57.265","volume":"55\u201357","author":"P Moonsri","year":"2008","unstructured":"Moonsri P, Watanesk S, Watanesk R, Niamsup H, Cheng Q, Deming RL (2008) Application of the PEGDE modified silk fibroin membrane to an amperometric glucose biosensor. Adv Mater Res 55\u201357:265. https:\/\/doi.org\/10.4028\/www.scientific.net\/AMR.55-57.265","journal-title":"Adv Mater Res"},{"issue":"8","key":"8049_CR18","doi-asserted-by":"publisher","first-page":"14222","DOI":"10.3390\/s140814222","volume":"14","author":"S Cinti","year":"2014","unstructured":"Cinti S, Arduini F, Moscone D, Palleschi G, Killard AJ (2014) Development of a hydrogen peroxide sensor based on screen-printed electrodes modified with inkjet-printed Prussian blue nanoparticles. Sensors (Basel) 14(8):14222\u201314234. https:\/\/doi.org\/10.3390\/s140814222","journal-title":"Sensors (Basel)"},{"key":"8049_CR19","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1016\/j.snb.2014.04.079","volume":"202","author":"X You","year":"2014","unstructured":"You X, Pak JJ (2014) Graphene-based field effect transistor enzymatic glucose biosensor using silk protein for enzyme immobilization and device substrate. Sens Actuators B Chem 202:1357. https:\/\/doi.org\/10.1016\/j.snb.2014.04.079","journal-title":"Sens Actuators B Chem"},{"key":"8049_CR20","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.carbpol.2016.08.060","volume":"155","author":"JAM Delezuk","year":"2017","unstructured":"Delezuk JAM, Pavinatto A, Moraes ML, Shimizu FM, Rodrigues VC, Campana-Filho SP, Ribeiro SJL, Oliveira ON (2017) Silk fibroin organization induced by chitosan in layer-by-layer films: application as a matrix in a biosensor. Carbohydr Polym 155:146. https:\/\/doi.org\/10.1016\/j.carbpol.2016.08.060","journal-title":"Carbohydr Polym"},{"key":"8049_CR21","doi-asserted-by":"publisher","DOI":"10.1002\/adsr.202500048","volume":"4","author":"KA Janus","year":"2025","unstructured":"Janus KA, Achtsnicht S, Isella B, Kopp A, Miyamoto K, Yoshinobu T, Keusgen M, Sch\u00f6ning MJ (2025) Silk-fibroin as biocompatible and bioresorbable enzyme immobilization matrix for screen-printed amperometric glucose biosensors. Adv Sens Res 4:e00048. https:\/\/doi.org\/10.1002\/adsr.202500048","journal-title":"Adv Sens Res"},{"key":"8049_CR22","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1111\/j.1600-065X.2009.00772.x","volume":"229","author":"R Rodriguez-Manzanet","year":"2009","unstructured":"Rodriguez-Manzanet R, DeKruyff R, Kuchroo VK, Umetsu DT (2009) The costimulatory role of TIM molecules. Immunol Rev 229:259. https:\/\/doi.org\/10.1111\/j.1600-065X.2009.00772.x","journal-title":"Immunol Rev"},{"key":"8049_CR23","doi-asserted-by":"publisher","first-page":"1504","DOI":"10.3892\/ijmm.2017.3150","volume":"4","author":"T Hu","year":"2017","unstructured":"Hu T, Fan X, Li M, Yunli C, Pingchang Y, Shuqi Q, Tao C, Litao Y, Liu Z (2017) TIM4-TIM1 interaction modulates Th2 pattern inflammation through enhancing SIRT1 expression. Int J Mol Med 4:1504. https:\/\/doi.org\/10.3892\/ijmm.2017.3150","journal-title":"Int J Mol Med"},{"key":"8049_CR24","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1691\/ph.2024.4612","volume":"79","author":"Q Li","year":"2024","unstructured":"Li Q, Shang H, Li XS, Wu GR, Qiang LX (2024) TIM-1 attenuates airway mucus hypersecretion and inflammation induced by cigarette smoke via the PI3K\/Akt signaling pathway. Pharmazie 79:10. https:\/\/doi.org\/10.1691\/ph.2024.4612","journal-title":"Pharmazie"},{"key":"8049_CR25","first-page":"207","volume":"33","author":"Y Wang","year":"2013","unstructured":"Wang Y, Martin TA, Jiang WG (2013) HAVcR-1 expression in human colorectal cancer and its effects on colorectal cancer cells in vitro. Anticancer Res 33:207 PMID: 23267147","journal-title":"Anticancer Res"},{"key":"8049_CR26","doi-asserted-by":"publisher","first-page":"121","DOI":"10.14670\/HH-11-817","volume":"32","author":"EJA Telford","year":"2017","unstructured":"Telford EJA, Jiang WG, Martin TA (2017) HAVcR-1 involvement in cancer progression. Histopathology 32:121\u2013128. https:\/\/doi.org\/10.14670\/HH-11-817","journal-title":"Histopathology"},{"key":"8049_CR27","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1186\/s12967-019-1931-2","volume":"17","author":"X Zheng","year":"2019","unstructured":"Zheng X, Xu K, Chen L, Zhou Y, Jiang J (2019) Prognostic value of TIM-1 expression in human non-small-cell lung cancer. J Transl Med 17:178. https:\/\/doi.org\/10.1186\/s12967-019-1931-2","journal-title":"J Transl Med"},{"key":"8049_CR28","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.01086","volume":"10","author":"X Kong","year":"2020","unstructured":"Kong X, Fu M, Niu X, Jiang H (2020) Comprehensive analysis of the expression, relationship to immune infiltration and prognosis of TIM-1 in cancer. Front Oncol 10:1086. https:\/\/doi.org\/10.3389\/fonc.2020.01086","journal-title":"Front Oncol"},{"key":"8049_CR29","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-022-09386-7","volume":"22","author":"L Chen","year":"2022","unstructured":"Chen L, Qing J, Xiao Y, Huang X, Chi Y, Chen Z (2022) TIM-1 promotes proliferation and metastasis, and inhibits apoptosis, in cervical cancer through the PI3K\/AKT\/P53 pathway. BMC Cancer 22:370. https:\/\/doi.org\/10.1186\/s12885-022-09386-7","journal-title":"BMC Cancer"},{"key":"8049_CR30","doi-asserted-by":"publisher","first-page":"102178","DOI":"10.1016\/j.genrep.2025.102178","volume":"39","author":"A Soltaninegar","year":"2025","unstructured":"Soltaninegar A, Ghahi FSJ, Hosseini S, Fard NA (2025) HAVCR1 in cancer: A systematic review of its dual-faceted role as a biomarker and therapeutic target. Gene Rep 39:102178. https:\/\/doi.org\/10.1016\/j.genrep.2025.102178","journal-title":"Gene Rep"},{"key":"8049_CR31","doi-asserted-by":"publisher","first-page":"125155","DOI":"10.1016\/J.TALANTA.2023.125155","volume":"267","author":"J Quinchia","year":"2024","unstructured":"Quinchia J, Bl\u00e1zquez-Garc\u00eda M, Torrente-Rodr\u00edguez RM, Ruiz-Valdepe\u00f1as Montiel V, Seraf\u00edn V, Rejas-Gonz\u00e1lez R, Montero-Calle A, Orozco J, Pingarr\u00f3n JM, Barderas R, Campuzano S (2024) Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion. Talanta 267:125155. https:\/\/doi.org\/10.1016\/J.TALANTA.2023.125155","journal-title":"Talanta"},{"key":"8049_CR32","doi-asserted-by":"publisher","first-page":"688","DOI":"10.1007\/s00604-025-07561-8","volume":"192","author":"A Cabrero-Mart\u00edn","year":"2025","unstructured":"Cabrero-Mart\u00edn A, Santiago S, Seraf\u00edn V, Pedrero M, Montero-Calle A, Pingarr\u00f3n JM, Barderas R, Campuzano S (2025) Multifunctional cerium nanolabels in electrochemical immunosensing with improved robustness and performance: determination of TIM-1 in colorectal cancer scenarios as a case study. Microchim Acta 192:688. https:\/\/doi.org\/10.1007\/s00604-025-07561-8","journal-title":"Microchim Acta"},{"key":"8049_CR33","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.electacta.2016.03.140","volume":"202","author":"G Mart\u00ednez-Garc\u00eda","year":"2016","unstructured":"Mart\u00ednez-Garc\u00eda G, Ag\u00fc\u00ed L, Y\u00e1\u00f1ez-Sede\u00f1o P, Pingarr\u00f3n JM (2016) Multiplexed electrochemical immunosensing of obesity-related hormones at grafted graphene-modified electrodes. Electrochim Acta 202:209. https:\/\/doi.org\/10.1016\/j.electacta.2016.03.140","journal-title":"Electrochim Acta"},{"key":"8049_CR34","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112238","volume":"163","author":"CA Razzino","year":"2020","unstructured":"Razzino CA, Seraf\u00edn V, Gamella M, Pedrero M, Montero-Calle A, Barderas R, Calero M, Lobo AO, Y\u00e1\u00f1ez-Sede\u00f1o P, Campuzano S, Pingarr\u00f3n JM (2020) An electrochemical immunosensor using gold nanoparticles-PAMAM-nanostructured screen-printed carbon electrodes for tau protein determination in plasma and brain tissues from Alzheimer patients. Biosens Bioelectron 163:112238. https:\/\/doi.org\/10.1016\/j.bios.2020.112238","journal-title":"Biosens Bioelectron"},{"issue":"8","key":"8049_CR35","doi-asserted-by":"publisher","first-page":"1785","DOI":"10.1177\/0003702817706368","volume":"71","author":"X Wu","year":"2017","unstructured":"Wu X, Wu X, Yang B, Shao M, Feng G (2017) Methanol-water-dependent structural changes of regenerated silk fibroin probed using terahertz spectroscopy. Appl Spectrosc 71(8):1785\u20131794. https:\/\/doi.org\/10.1177\/0003702817706368","journal-title":"Appl Spectrosc"},{"key":"8049_CR36","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2021.338907","volume":"1189","author":"S Gao","year":"2022","unstructured":"Gao S, Guis\u00e1n JM, Rocha-Martin J (2022) Oriented immobilization of antibodies onto sensing platforms - a critical review. Anal Chim Acta 1189:338907. https:\/\/doi.org\/10.1016\/j.aca.2021.338907","journal-title":"Anal Chim Acta"},{"issue":"7","key":"8049_CR37","doi-asserted-by":"publisher","first-page":"2782","DOI":"10.1016\/j.actbio.2011.03.001","volume":"7","author":"Q Lu","year":"2011","unstructured":"Lu Q, Wang X, Zhu H, Kaplan DL (2011) Surface immobilization of antibody on silk fibroin through conformational transition. Acta Biomater 7(7):2782\u20132786. https:\/\/doi.org\/10.1016\/j.actbio.2011.03.001","journal-title":"Acta Biomater"},{"key":"8049_CR38","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1002\/polb.1994.090320519","volume":"32","author":"M Tsukada","year":"1994","unstructured":"Tsukada M, Gotoh Y, Nagura M, Minoura N, Kasai N, Freddi G (1994) Structural changes of silk fibroin membranes induced by immersion in methanol aqueous solutions. J Polym Sci B Polym Phys 32:961. https:\/\/doi.org\/10.1002\/polb.1994.090320519","journal-title":"J Polym Sci B Polym Phys"},{"key":"8049_CR39","doi-asserted-by":"publisher","first-page":"21361","DOI":"10.1073\/pnas.1911563116","volume":"116","author":"Y Wang","year":"2019","unstructured":"Wang Y, Kim BJ, Peng B, Li W, Wang Y, Li M, Omenetto FG (2019) Controlling silk fibroin conformation for dynamic, responsive, multifunctional, micropatterned surfaces. Proc Natl Acad Sci 116:21361. https:\/\/doi.org\/10.1073\/pnas.1911563116","journal-title":"Proc Natl Acad Sci"},{"key":"8049_CR40","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.polymdegradstab.2014.04.008","volume":"105","author":"MA Koperska","year":"2014","unstructured":"Koperska MA, Pawcenis D, Bagniuk J, Zaitz MM, Missori M, \u0141ojewski T, \u0141ojewska J (2014) Degradation markers of fibroin in silk through infrared spectroscopy. Polym Degrad Stab 105:185. https:\/\/doi.org\/10.1016\/j.polymdegradstab.2014.04.008","journal-title":"Polym Degrad Stab"},{"key":"8049_CR41","doi-asserted-by":"publisher","first-page":"3423","DOI":"10.1021\/ja00324a007","volume":"106","author":"K Itaya","year":"1984","unstructured":"Itaya K, Shoji N, Uchida I (1984) Catalysis of the reduction of molecular oxygen to water at Prussian blue modified electrodes. J Am Chem Soc 106:3423. https:\/\/doi.org\/10.1021\/ja00324a007","journal-title":"J Am Chem Soc"},{"key":"8049_CR42","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.talanta.2011.03.028","volume":"85","author":"Y Jiang","year":"2011","unstructured":"Jiang Y, Zhang X, Shan C, Hua S, Zhang Q, Bai X, Dan L, Niu L (2011) Functionalization of graphene with electrodeposited Prussian Blue towards amperometric sensing application. Talanta 85:76. https:\/\/doi.org\/10.1016\/j.talanta.2011.03.028","journal-title":"Talanta"},{"key":"8049_CR43","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-014-1433-0","volume":"182","author":"J Yang","year":"2015","unstructured":"Yang J, Lin M, Cho M, Lee Y (2015) Determination of hydrogen peroxide using a Prussian Blue modified macroporous gold electrode. Microchim Acta 182:1089. https:\/\/doi.org\/10.1007\/s00604-014-1433-0","journal-title":"Microchim Acta"},{"key":"8049_CR44","doi-asserted-by":"publisher","first-page":"6593","DOI":"10.1021\/acsomega.4c07455","volume":"10","author":"MAP Papi","year":"2025","unstructured":"Papi MAP, Kalinke C, Soccol CR, Soccol VT, Beir\u00e3o BCB, Bergamini MF, Marcolino-J\u00fanior LH (2025) Electrochemical paper-based analytical device (e-PAD) using immobilized Prussian Blue and antibodies for the diagnosis of Leishmania. ACS Omega 10:6593. https:\/\/doi.org\/10.1021\/acsomega.4c07455","journal-title":"ACS Omega"},{"key":"8049_CR45","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1021\/ed075p881","volume":"75","author":"JJ Garc\u00eda-Jare\u00f1o","year":"1998","unstructured":"Garc\u00eda-Jare\u00f1o JJ, Benito D, Navarro-Laboulais J, Vicente F (1998) Electrochemical behavior of electrodeposited Prussian Blue films on ITO electrode: an attractive laboratory experience. J Chem Educ 75:881. https:\/\/doi.org\/10.1021\/ed075p881","journal-title":"J Chem Educ"},{"key":"8049_CR46","doi-asserted-by":"publisher","first-page":"11302","DOI":"10.1021\/jacs.8b05223","volume":"140","author":"MA Komkova","year":"2018","unstructured":"Komkova MA, Karyakina EE, Karyakin AA (2018) Catalytically synthesized Prussian Blue nanoparticles defeating natural enzyme peroxidase. J Am Chem Soc 140:11302. https:\/\/doi.org\/10.1021\/jacs.8b05223","journal-title":"J Am Chem Soc"},{"key":"8049_CR47","doi-asserted-by":"publisher","DOI":"10.3390\/nano15010041","volume":"15","author":"UB Gunatilake","year":"2025","unstructured":"Gunatilake UB, P\u00e9rez-L\u00f3pez B, Urpi M, Prat-Trunas J, Carrera-Cardona G, F\u00e9lix G, Sene S, Beaudhuin M, Dupin JC, Allouche J, Guari Y, Larionova J, Baldrich E (2025) Peroxidase (POD) mimicking activity of different types of poly(ethyleneimine)-mediated Prussian Blue nanoparticles. Nanomaterials 15:41. https:\/\/doi.org\/10.3390\/nano15010041","journal-title":"Nanomaterials"},{"key":"8049_CR48","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-39687-1","volume":"13","author":"D Morales-Urrea","year":"2023","unstructured":"Morales-Urrea D, L\u00f3pez-C\u00f3rdoba A, Contreras EM (2023) Inactivation kinetics of horseradish peroxidase (HRP) by hydrogen peroxide. Sci Rep 13:13363. https:\/\/doi.org\/10.1038\/s41598-023-39687-1","journal-title":"Sci Rep"},{"key":"8049_CR49","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201800347","volume":"7","author":"Z Qin","year":"2018","unstructured":"Qin Z, Li Y, Gu N (2018) Progress in applications of Prussian Blue nanoparticles in biomedicine. Adv Healthc Mater 7:1800347. https:\/\/doi.org\/10.1002\/adhm.201800347","journal-title":"Adv Healthc Mater"},{"key":"8049_CR50","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1016\/J.SNB.2019.01.012","volume":"284","author":"V Seraf\u00edn","year":"2019","unstructured":"Seraf\u00edn V, Valverde A, Mart\u00ednez-Garc\u00eda G, Mart\u00ednez-Peri\u00f1\u00e1n E, Comba F, Garranzo-Asensio M, Barderas R, Y\u00e1\u00f1ez-Sede\u00f1o P, Campuzano S, Pingarr\u00f3n JM (2019) Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor \u03b12 in colorectal cells and tumor tissues with different metastatic potential. Sens Actuators B Chem 284:711. https:\/\/doi.org\/10.1016\/J.SNB.2019.01.012","journal-title":"Sens Actuators B Chem"},{"key":"8049_CR51","doi-asserted-by":"publisher","first-page":"1293","DOI":"10.35124\/bca.2020.20.1.1293","volume":"20","author":"A Lateef Alturfa","year":"2020","unstructured":"Lateef Alturfa A, Alsafi KA, Altimimi RJ (2020) Gene polymorphism and saliva concentrations of matrix metalloproteinase-9 and its tissue inhibitor in Iraqi patients with chronic periodontitis. Biochem Cell Arch 20:1293. https:\/\/doi.org\/10.35124\/bca.2020.20.1.1293","journal-title":"Biochem Cell Arch"},{"key":"8049_CR52","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1007\/s10517-019-04533-w","volume":"167","author":"NE Kushlinskii","year":"2019","unstructured":"Kushlinskii NE, Gershtein ES, Naberezhnov DS, Taipov MA, Bezhanova SD, Pushkar DY, Matveev VB, Stilidi IS (2019) Kidney injury molecule-1 (KIM-1) in blood plasma of patients with clear-cell carcinoma. Bull Exp Biol Med 167:388. https:\/\/doi.org\/10.1007\/s10517-019-04533-w","journal-title":"Bull Exp Biol Med"},{"key":"8049_CR53","unstructured":"ThermoFisher Scientific (n (2025) d.) Human TIM-1 (HAVCR1) ELISA Kit. ThermoFisher Scientific. https:\/\/www.thermofisher.com\/elisa\/product\/Human-TIM-1-HAVCR1-ELISA-Kit\/EHHAVCR1. Accessed Nov 28"},{"key":"8049_CR54","unstructured":"RayBiotech (n.d.) Human TIM-1 ELISA (ELH-TIM1). RayBiotech. https:\/\/www.raybiotech.com\/human-tim-1-elisa-elh-tim1. Accessed 28 Nov 2025"},{"key":"8049_CR55","unstructured":"Abcam, Human KIM-1 ELISA Kit (TIM-1). Abcam. https:\/\/www.abcam.com\/en-us\/products\/elisa-kits\/human-kim1-elisa-kit-tim-1-ab235081. Accessed 28 Nov 2025"},{"key":"8049_CR56","unstructured":"R&D Systems (n.d.) Human urinary TIM-1 \/ KIM-1 (HAVCR) Quantikine ELISA Kit. R&D Systems. https:\/\/www.rndsystems.com\/products\/human-urinary-tim-1-kim-1-havcr-quantikine-elisa-kit_dkm100. Accessed 28 Nov 2025"},{"key":"8049_CR57","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2021.152093","volume":"578","author":"H Boyac\u0131o\u011flu","year":"2022","unstructured":"Boyac\u0131o\u011flu H, Yola BB, Karaman C, Karaman O, Atar N, Yola ML (2022) <article-title update=\u201cadded\u201d>A novel electrochemical kidney injury molecule-1 (KIM-1) immunosensor based covalent organic frameworks-gold nanoparticles composite and porous NiCo2S4@CeO2 microspheres: the monitoring of acute kidney injury. Appl Surf Sci 578:152093. https:\/\/doi.org\/10.1016\/j.apsusc.2021.152093","journal-title":"Appl Surf Sci"},{"key":"8049_CR58","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.bios.2018.05.032","volume":"116","author":"H Yang","year":"2018","unstructured":"Yang H, Wang H, Xiong C, Chai Y, Yuan R (2018) Highly sensitive electrochemiluminescence immunosensor based on ABEI\/H2O2 system with PFO dots as enhancer for detection of kidney injury molecule-1. Biosens Bioelectron 116:16. https:\/\/doi.org\/10.1016\/j.bios.2018.05.032","journal-title":"Biosens Bioelectron"},{"key":"8049_CR59","doi-asserted-by":"publisher","first-page":"821","DOI":"10.3892\/ijo_00000208","volume":"34","author":"QW Ben","year":"2009","unstructured":"Ben QW, Zhao Z, Ge SF, Zhou J, Yuan F, Yuan YZ (2009) Circulating levels of periostin may help identify patients with more aggressive colorectal cancer. Int J Oncol 34:821. https:\/\/doi.org\/10.3892\/ijo_00000208","journal-title":"Int J Oncol"},{"key":"8049_CR60","doi-asserted-by":"publisher","first-page":"2780","DOI":"10.1158\/0008-5472.CAN-11-4090","volume":"72","author":"R Barderas","year":"2012","unstructured":"Barderas R, Bartolom\u00e9 RA, Fernandez-Ace\u00f1ero MJ, Torres S, Casal JI (2012) High expression of IL-13 receptor \u03b12 in colorectal cancer is associated with invasion, liver metastasis, and poor prognosis. Cancer Res 72:2780. https:\/\/doi.org\/10.1158\/0008-5472.CAN-11-4090","journal-title":"Cancer Res"},{"key":"8049_CR61","doi-asserted-by":"publisher","first-page":"18390","DOI":"10.3748\/wjg.v20.i48.18390","volume":"20","author":"OA Al Obeed","year":"2014","unstructured":"Al Obeed OA, Alkhayal KA, Al Sheikh A, Zubaidi AM, Vaali-Mohammed MA, Boushey R, Mckerrow JH, Abdulla MH (2014) Increased expression of tumor necrosis factor-\u03b1 is associated with advanced colorectal cancer stages. World J Gastroenterol 20:18390. https:\/\/doi.org\/10.3748\/wjg.v20.i48.18390","journal-title":"World J Gastroenterol"},{"key":"8049_CR62","doi-asserted-by":"publisher","first-page":"12108","DOI":"10.3390\/ijms160612108","volume":"16","author":"Z Li","year":"2015","unstructured":"Li Z, Zhang X, Yang Y, Yang S, Dong Z, Du L, Wang L, Wang C (2015) Periostin expression and its prognostic value for colorectal cancer. Int J Mol Sci 16:12108. https:\/\/doi.org\/10.3390\/ijms160612108","journal-title":"Int J Mol Sci"},{"key":"8049_CR63","doi-asserted-by":"publisher","first-page":"547","DOI":"10.21147\/j.issn.1000-9604.2019.03.16","volume":"31","author":"X Deng","year":"2019","unstructured":"Deng X, Ao S, Hou J, Li Z, Lei Y, Lyu G (2019) Prognostic significance of periostin in colorectal cancer. Chin J Cancer Res 31:547. https:\/\/doi.org\/10.21147\/j.issn.1000-9604.2019.03.16","journal-title":"Chin J Cancer Res"},{"key":"8049_CR64","doi-asserted-by":"publisher","first-page":"100224","DOI":"10.1016\/j.mcpsp.2021.100224","volume":"4","author":"LF Warsinggih","year":"2021","unstructured":"Warsinggih LF, Labeda I, Lusikooy RE, Mappincara, Faruk M (2021) Relationship of tumor necrosis factor alpha levels in plasma toward the stage and differentiation degree in colorectal cancer. Med Clin Pract 4:100224. https:\/\/doi.org\/10.1016\/j.mcpsp.2021.100224","journal-title":"Med Clin Pract"},{"key":"8049_CR65","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/0001-8686(86)80012-4","volume":"25","author":"W Norde","year":"1986","unstructured":"Norde W (1986) Adsorption of proteins from solution at the solid\u2013liquid interface. Adv Colloid Interface Sci 25:267. https:\/\/doi.org\/10.1016\/0001-8686(86)80012-4","journal-title":"Adv Colloid Interface Sci"},{"key":"8049_CR66","unstructured":"Cai CCY, Mofienski JJ (2012) Disposable sensor for the electrochemical detection of hemoglobin. ES2707877T3"},{"key":"8049_CR67","doi-asserted-by":"publisher","first-page":"1735","DOI":"10.1002\/slct.202004180","volume":"6","author":"S Lee","year":"2021","unstructured":"Lee S, Kim SH, Jo YY, Ju WT, Kim HB, Kweon HY (2021) Conformation transition kinetics of silk fibroin in aqueous solution explored using circular dichroism spectroscopy. ChemistrySelect 6:1735. https:\/\/doi.org\/10.1002\/slct.202004180","journal-title":"ChemistrySelect"},{"key":"8049_CR68","doi-asserted-by":"publisher","DOI":"10.1002\/mame.202200700","volume":"308","author":"XQ Qian","year":"2023","unstructured":"Qian XQ, Wang HY, Jing FY, Zhang YQ, Xu X, Shu Y (2023) A novel immobilization method of urease in silk fibroin scaffolds by annealing at constant temperature and humidity. Macromol Mater Eng 308:2200700. https:\/\/doi.org\/10.1002\/mame.202200700","journal-title":"Macromol Mater Eng"},{"issue":"31","key":"8049_CR69","doi-asserted-by":"publisher","first-page":"7766","DOI":"10.1016\/j.biomaterials.2013.06.039","volume":"34","author":"NA Guziewicz","year":"2013","unstructured":"Guziewicz NA, Massetti AJ, Perez-Ramirez BJ, Kaplan DL (2013) Mechanisms of monoclonal antibody stabilization and release from silk biomaterials. Biomaterials 34(31):7766\u20137775. https:\/\/doi.org\/10.1016\/j.biomaterials.2013.06.039","journal-title":"Biomaterials"},{"key":"8049_CR70","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1016\/j.jconrel.2015.09.037","volume":"219","author":"AB Li","year":"2015","unstructured":"Li AB, Kluge JA, Guziewicz NA, Omenetto FG, Kaplan DL (2015) Silk-based stabilization of biomacromolecules. J Control Release 219:416\u2013430. https:\/\/doi.org\/10.1016\/j.jconrel.2015.09.037","journal-title":"J Control Release"},{"issue":"40","key":"8049_CR71","doi-asserted-by":"publisher","first-page":"26493","DOI":"10.1021\/acsami.6b07362","volume":"8","author":"C Wang","year":"2016","unstructured":"Wang C, Luan J, Tadepalli S, Liu KK, Morrissey JJ, Kharasch ED, Naik RR, Singamaneni S (2016) Silk-encapsulated plasmonic biochips with enhanced thermal stability. ACS Appl Mater Interfaces 8(40):26493\u201326500. https:\/\/doi.org\/10.1021\/acsami.6b07362","journal-title":"ACS Appl Mater Interfaces"}],"container-title":["Microchimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-026-08049-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00604-026-08049-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-026-08049-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T10:55:47Z","timestamp":1778496947000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00604-026-08049-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,20]]},"references-count":71,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["8049"],"URL":"https:\/\/doi.org\/10.1007\/s00604-026-08049-9","relation":{},"ISSN":["0026-3672","1436-5073"],"issn-type":[{"value":"0026-3672","type":"print"},{"value":"1436-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,20]]},"assertion":[{"value":"26 February 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The plasma samples from healthy individuals, patients with premalignant lesions -low- and high-grade colorectal adenomas- and with early-stage CRC handled and analyzed were provided by biobank of Hospital Cl\u00ednico San Carlos after approval of the corresponding Ethical Committee (CEI PI 13_2020-v2). Written informed consent was obtained from all study participants.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Human Ethics and Consent to Participate"}},{"value":"not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Clinical trial number"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"322"}}