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Here, a green, magneto-electrochemical biosensing strategy is reported for the selective detection of\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    based on pathogen-specific nuclease activity. Uniform organic\u2013inorganic hybrid polyhedral oligomeric silsesquioxane (POSS) nanoparticles were synthesized via an ultrafast UV-initiated emulsion polymerization within 5\u00a0min using an eco-friendly approach. The nanoparticles were sequentially functionalized by in situ deposition of superparamagnetic iron oxide nanoparticles and biomimetic polydopamine coating, enabling robust and high-density immobilization of nuclease-responsive oligonucleotide probes. The resulting PDA@SPION\/POSS nanohybrids exhibit controlled size, preserved structural integrity, and strong superparamagnetic behavior, allowing efficient magnetic manipulation and electrochemical signal transduction. Upon exposure to\n                    <jats:italic>S. pneumoniae<\/jats:italic>\n                    , nuclease-mediated probe cleavage produces a pronounced electrochemical response, enabling label-free detection over a wide dynamic range (10\n                    <jats:sup>2<\/jats:sup>\n                    \u201310\u2078 CFU mL\u207b\u00b9) with a detection limit of 10\n                    <jats:sup>2<\/jats:sup>\n                    CFU mL\u207b\u00b9. High selectivity against non-target bacteria highlights the specificity of the enzymatic recognition mechanism. This work establishes a sustainable and amplification-free biosensing platform with strong potential for rapid clinical diagnostics.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1007\/s00604-026-08087-3","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T15:12:18Z","timestamp":1777734738000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Magneto-electrochemical biosensing for pathogen detection using nuclease-responsive nanohybrids"],"prefix":"10.1007","volume":"193","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2586-6770","authenticated-orcid":false,"given":"Gunes","family":"Kibar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5331-7253","authenticated-orcid":false,"given":"Murat","family":"Kavruk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6308-8087","authenticated-orcid":false,"given":"Frank J.","family":"Hernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4285-2933","authenticated-orcid":false,"given":"Baris A.","family":"Borsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9056-0025","authenticated-orcid":false,"given":"Ali Dogan","family":"Dursun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7659-5990","authenticated-orcid":false,"given":"Veli Cengiz","family":"Ozalp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,2]]},"reference":[{"key":"8087_CR1","unstructured":"CDC (2024) About pneumococcal disease | Pneumococcal | CDC. https:\/\/www.cdc.gov\/pneumococcal\/about\/index.html"},{"key":"8087_CR2","doi-asserted-by":"publisher","first-page":"1736","DOI":"10.1016\/S0140-6736(18)32203-7","volume":"392","author":"GA Roth","year":"2018","unstructured":"Roth GA, Abate D, Abate KH et al (2018) Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980\u20132017: a systematic analysis for the Global Burden of Disease Study 2017. 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