{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T08:37:39Z","timestamp":1786523859129,"version":"build-2736575974"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Research Project of Jiangsu Association of Chinese Medicine","award":["CYTF 2024050"],"award-info":[{"award-number":["CYTF 2024050"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microchim Acta"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1007\/s00604-026-08287-x","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T09:42:40Z","timestamp":1784713360000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual-mode fluorescence and UV-vis aptasensor for hepatocellular carcinoma detection using magnetic separation"],"prefix":"10.1007","volume":"193","author":[{"given":"Yiwen","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liquan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danhao","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoshen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinbing","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoqi","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"issue":"5","key":"8287_CR1","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1016\/j.jhep.2024.10.031","volume":"82","author":"EY Tan","year":"2025","unstructured":"Tan EY, Danpanichkul P, Yong JN et al (2025) Liver cancer in 2021: global burden of disease study. J Hepatol 82(5):851\u2013860","journal-title":"J Hepatol"},{"issue":"7","key":"8287_CR2","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/j.cmet.2022.05.003","volume":"34","author":"DQ Huang","year":"2022","unstructured":"Huang DQ, Singal AG, Kono Y et al (2022) Changing global epidemiology of liver cancer from 2010 to 2019: NASH is the fastest growing cause of liver cancer. Cell Metabol 34(7):969\u2013977e2","journal-title":"Cell Metabol"},{"issue":"4","key":"8287_CR3","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1007\/s10620-019-05537-2","volume":"64","author":"M Sayiner","year":"2019","unstructured":"Sayiner M, Golabi P, Younossi ZM (2019) Disease burden of hepatocellular carcinoma: a global perspective. Dig Dis Sci 64(4):910\u2013917","journal-title":"Dig Dis Sci"},{"issue":"2","key":"8287_CR4","doi-asserted-by":"publisher","first-page":"358","DOI":"10.3350\/cmh.2023.0092","volume":"29","author":"P Konyn","year":"2023","unstructured":"Konyn P, Ahmed A, Kim D (2023) The current trends in the health burden of primary liver cancer across the globe. Clin Mol Hepatol 29(2):358","journal-title":"Clin Mol Hepatol"},{"issue":"11","key":"8287_CR5","first-page":"151","volume":"15","author":"M Lampimukhi","year":"2023","unstructured":"Lampimukhi M, Qassim T, Venu R et al (2023) A review of incidence and related risk factors in the development of hepatocellular carcinoma. Cureus 15(11):151","journal-title":"Cureus"},{"issue":"12","key":"8287_CR6","doi-asserted-by":"publisher","first-page":"864","DOI":"10.1038\/s41571-023-00825-3","volume":"20","author":"AG Singal","year":"2023","unstructured":"Singal AG, Kanwal F, Llovet JM (2023) Global trends in hepatocellular carcinoma epidemiology: implications for screening, prevention and therapy. Nat reviews Clin Oncol 20(12):864\u2013884","journal-title":"Nat reviews Clin Oncol"},{"issue":"1","key":"8287_CR7","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1186\/s12943-024-02101-z","volume":"23","author":"YT Chan","year":"2024","unstructured":"Chan YT, Zhang C, Wu J et al (2024) Biomarkers for diagnosis and therapeutic options in hepatocellular carcinoma. Mol Cancer 23(1):189","journal-title":"Mol Cancer"},{"issue":"12","key":"8287_CR8","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.12998\/wjcc.v7.i12.1367","volume":"7","author":"LA Balaceanu","year":"2019","unstructured":"Balaceanu LA (2019) Biomarkers vs imaging in the early detection of hepatocellular carcinoma and prognosis. World J Clin Cases 7(12):1367","journal-title":"World J Clin Cases"},{"issue":"10","key":"8287_CR9","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1038\/s41575-022-00620-y","volume":"19","author":"P Johnson","year":"2022","unstructured":"Johnson P, Zhou Q, Dao DY et al (2022) Circulating biomarkers in the diagnosis and management of hepatocellular carcinoma. Nat reviews Gastroenterol Hepatol 19(10):670\u2013681","journal-title":"Nat reviews Gastroenterol Hepatol"},{"issue":"12","key":"8287_CR10","doi-asserted-by":"publisher","first-page":"1972","DOI":"10.1002\/hep4.1823","volume":"5","author":"N Adeniji","year":"2021","unstructured":"Adeniji N, Dhanasekaran R (2021) Current and emerging tools for hepatocellular carcinoma surveillance. Hepatol Commun 5(12):1972\u20131986","journal-title":"Hepatol Commun"},{"issue":"19","key":"8287_CR11","doi-asserted-by":"publisher","first-page":"4647","DOI":"10.3390\/cancers14194647","volume":"14","author":"L Cerrito","year":"2022","unstructured":"Cerrito L, Ainora ME, Mosoni C et al (2022) Prognostic role of molecular and imaging biomarkers for predicting advanced hepatocellular carcinoma treatment efficacy. Cancers 14(19):4647","journal-title":"Cancers"},{"issue":"22","key":"8287_CR12","doi-asserted-by":"publisher","first-page":"1708","DOI":"10.2174\/0113816128298875240321073907","volume":"30","author":"KK Sharma","year":"2024","unstructured":"Sharma KK, Mohsin M, Mittal P et al (2024) Diagnosis of the initial stage of hepatocellular carcinoma: a review. Curr Pharm Design 30(22):1708\u20131724","journal-title":"Curr Pharm Design"},{"issue":"15","key":"8287_CR13","doi-asserted-by":"publisher","first-page":"3880","DOI":"10.3390\/cancers15153880","volume":"15","author":"H\u00c7 Y\u0131ld\u0131r\u0131m","year":"2023","unstructured":"Y\u0131ld\u0131r\u0131m H\u00c7, Kavgaci G, Chalabiyev E et al (2023) Advances in the early detection of hepatobiliary cancers. Cancers 15(15):3880","journal-title":"Cancers"},{"issue":"2","key":"8287_CR14","doi-asserted-by":"publisher","first-page":"216","DOI":"10.3748\/wjg.v28.i2.216","volume":"28","author":"H Hanif","year":"2022","unstructured":"Hanif H, Ali MJ, Susheela AT et al (2022) Update on the applications and limitations of alpha-fetoprotein for hepatocellular carcinoma. World J Gastroenterol 28(2):216","journal-title":"World J Gastroenterol"},{"issue":"1","key":"8287_CR15","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1186\/s40001-025-03003-6","volume":"30","author":"S Zafar","year":"2025","unstructured":"Zafar S, Hafeez A, Shah H et al (2025) Emerging biomarkers for early cancer detection and diagnosis: Challenges, innovations, and clinical perspectives. Eur J Med Res 30(1):760","journal-title":"Eur J Med Res"},{"issue":"1","key":"8287_CR16","doi-asserted-by":"publisher","first-page":"37","DOI":"10.3390\/s24010037","volume":"24","author":"S Das","year":"2023","unstructured":"Das S, Dey MK, Devireddy R et al (2023) Biomarkers in cancer detection, diagnosis, and prognosis. Sensors 24(1):37","journal-title":"Sensors"},{"issue":"1","key":"8287_CR17","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1002\/biof.2001","volume":"50","author":"Y Liu","year":"2024","unstructured":"Liu Y, Lu X, Chen M et al (2024) Advances in screening, synthesis, modification, and biomedical applications of peptides and peptide aptamers. BioFactors 50(1):33\u201357","journal-title":"BioFactors"},{"key":"8287_CR18","doi-asserted-by":"publisher","first-page":"2427","DOI":"10.2147\/IJN.S507715","volume":"12","author":"H Yu","year":"2025","unstructured":"Yu H, Yu J, Yao G (2025) Recent advances in aptamers-based nanosystems for diagnosis and therapy of cardiovascular diseases: an updated review. Int J Nanomed 12:2427\u20132443","journal-title":"Int J Nanomed"},{"issue":"37","key":"8287_CR19","doi-asserted-by":"publisher","first-page":"5850","DOI":"10.2174\/0929867329666220224155037","volume":"29","author":"DA Sousa","year":"2022","unstructured":"Sousa DA, Carneiro M, Ferreira D et al (2022) Recent advances in the selection of cancer-specific aptamers for the development of biosensors. Curr Med Chem 29(37):5850\u20135880","journal-title":"Curr Med Chem"},{"issue":"10","key":"8287_CR20","doi-asserted-by":"publisher","first-page":"e202301008","DOI":"10.1002\/cbdv.202301008","volume":"20","author":"PUA Raj","year":"2023","unstructured":"Raj PUA, John G (2023) Aptamers: features, synthesis and applications. Chem Biodivers 20(10):e202301008","journal-title":"Chem Biodivers"},{"issue":"1","key":"8287_CR21","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1186\/s12951-020-00613-6","volume":"18","author":"C Tang","year":"2020","unstructured":"Tang C, He Z, Liu H et al (2020) Application of magnetic nanoparticles in nucleic acid detection. J Nanobiotechnol 18(1):62","journal-title":"J Nanobiotechnol"},{"issue":"1","key":"8287_CR22","doi-asserted-by":"publisher","first-page":"29","DOI":"10.3390\/bios15010029","volume":"15","author":"L Zheng","year":"2025","unstructured":"Zheng L, Ye Q, Wang M et al (2025) Research progress in small-molecule detection using aptamer-based SERS techniques. Biosensors 15(1):29","journal-title":"Biosensors"},{"issue":"4","key":"8287_CR23","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.3390\/s18041041","volume":"18","author":"H Modh","year":"2018","unstructured":"Modh H, Scheper T, Walter JG (2018) Aptamer-modified magnetic beads in biosensing. Sensors 18(4):1041","journal-title":"Sensors"},{"issue":"24","key":"8287_CR24","doi-asserted-by":"publisher","first-page":"5717","DOI":"10.1039\/C9CS00174C","volume":"48","author":"MK Masud","year":"2019","unstructured":"Masud MK, Na J, Younus M et al (2019) Superparamagnetic nanoarchitectures for disease-specific biomarker detection. Chem Soc Rev 48(24):5717\u20135751","journal-title":"Chem Soc Rev"},{"issue":"27","key":"8287_CR25","doi-asserted-by":"publisher","first-page":"31418","DOI":"10.1021\/acsami.1c05355","volume":"13","author":"N Soda","year":"2021","unstructured":"Soda N, Gonzaga ZJ, Chen S et al (2021) Bioengineered polymer nanobeads for isolation and electrochemical detection of cancer biomarkers. ACS Appl Mater Interfaces 13(27):31418\u201331430","journal-title":"ACS Appl Mater Interfaces"},{"issue":"30","key":"8287_CR26","doi-asserted-by":"publisher","first-page":"2004734","DOI":"10.1002\/adma.202004734","volume":"33","author":"X Liu","year":"2021","unstructured":"Liu X, Wu W, Cui D et al (2021) Functional micro\/nanomaterials for multiplexed biodetection. Adv Mater 33(30):2004734","journal-title":"Adv Mater"},{"issue":"19","key":"8287_CR27","doi-asserted-by":"publisher","first-page":"4551","DOI":"10.3390\/molecules29194551","volume":"29","author":"X Ma","year":"2024","unstructured":"Ma X, Ge Y, Xia N (2024) Overview of the design and application of dual-signal immunoassays. Molecules 29(19):4551","journal-title":"Molecules"},{"key":"8287_CR28","doi-asserted-by":"publisher","first-page":"115007","DOI":"10.1016\/j.microc.2025.115007","volume":"10","author":"S Mansouri","year":"2025","unstructured":"Mansouri S, Alharbi Y, Alqahtani A (2025) Triple-mode biosensing strategies: A critical review of design principles, performance metrics, and applications for ultrasensitive detection. Microchem J 10:115007","journal-title":"Microchem J"},{"issue":"39","key":"8287_CR29","doi-asserted-by":"publisher","first-page":"2403122","DOI":"10.1002\/adfm.202403122","volume":"34","author":"Q Han","year":"2024","unstructured":"Han Q, Wang H, Wang J (2024) Multi-mode\/signal biosensors: Electrochemical integrated sensing techniques. Adv Funct Mater 34(39):2403122","journal-title":"Adv Funct Mater"},{"key":"8287_CR30","doi-asserted-by":"publisher","first-page":"137890","DOI":"10.1016\/j.snb.2025.137890","volume":"440","author":"J Hu","year":"2025","unstructured":"Hu J, Wu C, Liu B et al (2025) A dual-mode paper-based humidity sensor: Optical and electrical dual-signal sensing platform for multifunctional applications. Sens Actuators B 440:137890","journal-title":"Sens Actuators B"},{"key":"8287_CR31","doi-asserted-by":"publisher","first-page":"140190","DOI":"10.1016\/j.foodchem.2024.140190","volume":"457","author":"C Wang","year":"2024","unstructured":"Wang C, Gu C, Zhao X et al (2024) Self-designed portable dual-mode fluorescence device with custom python-based analysis software for rapid detection via dual-color FRET aptasensor with IoT capabilities. Food Chem 457:140190","journal-title":"Food Chem"},{"issue":"12","key":"8287_CR32","doi-asserted-by":"publisher","first-page":"3390","DOI":"10.1007\/s12161-022-02366-4","volume":"15","author":"C Wang","year":"2022","unstructured":"Wang C, Zhao X, Gu C et al (2022) Fabrication of a versatile aptasensing chip for aflatoxin B1 in photothermal and electrochemical dual modes. Food Anal Methods 15(12):3390\u20133399","journal-title":"Food Anal Methods"},{"issue":"4","key":"8287_CR33","doi-asserted-by":"publisher","first-page":"e33434","DOI":"10.1371\/journal.pone.0033434","volume":"7","author":"L Meng","year":"2012","unstructured":"Meng L, Yang L, Zhao X et al (2012) Targeted delivery of chemotherapy agents using a liver cancer-specific aptamer. PLoS ONE 7(4):e33434","journal-title":"PLoS ONE"},{"issue":"5","key":"8287_CR34","doi-asserted-by":"publisher","first-page":"2688","DOI":"10.3892\/or.2017.5527","volume":"37","author":"Z Lai","year":"2017","unstructured":"Lai Z, Tan J, Wan R et al (2017) An \u2018activatable\u2019 aptamer-based fluorescence probe for the detection of HepG2 cells. Oncol Rep 37(5):2688\u20132694","journal-title":"Oncol Rep"},{"issue":"5","key":"8287_CR35","doi-asserted-by":"publisher","first-page":"2844","DOI":"10.1021\/acs.analchem.6b03971","volume":"89","author":"R Deng","year":"2017","unstructured":"Deng R, Qu H, Liang L et al (2017) Tracing the therapeutic process of targeted aptamer\/drug conjugate on cancer cells by surface-enhanced Raman scattering spectroscopy. Anal Chem 89(5):2844\u20132851","journal-title":"Anal Chem"},{"key":"8287_CR36","first-page":"115","volume":"15","author":"EI McIvor","year":"2017","unstructured":"McIvor EI (2017) Aptamer targeted drug delivery and progress towards novel biomarker identification in hepatocellular carcinoma cells. Diss 15:115","journal-title":"Diss"},{"issue":"4","key":"8287_CR37","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1007\/s11468-021-01381-1","volume":"16","author":"K Khurana","year":"2021","unstructured":"Khurana K, Jaggi N (2021) Localized surface plasmonic properties of Au and Ag nanoparticles for sensors: a review. Plasmonics 16(4):981\u2013999","journal-title":"Plasmonics"},{"key":"8287_CR38","first-page":"118626","volume":"9","author":"M Dadmehr","year":"2025","unstructured":"Dadmehr M, Allahverdi M, Kalat NK et al (2025) Molecular biosensors for detection of miRNA and its application in medical diagnosis. TRAC Trends Anal Chem 9:118626","journal-title":"TRAC Trends Anal Chem"},{"issue":"3","key":"8287_CR39","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1007\/s00604-025-07018-y","volume":"192","author":"F Hosseini","year":"2025","unstructured":"Hosseini F, Dadmehr M, Hosseini M (2025) Fluorescence-based detection of Let-7a miRNA through HCR-based approach upon the in situ interaction of AuNPs@ CdS QDs and FRET mechanism. Microchim Acta 192(3):153","journal-title":"Microchim Acta"},{"key":"8287_CR40","doi-asserted-by":"publisher","first-page":"111127","DOI":"10.1016\/j.microc.2024.111127","volume":"205","author":"M Dadmehr","year":"2024","unstructured":"Dadmehr M, Korouzhdehi B, Hosseini M (2024) Multimodal lab-on-a-tube detection of AFB1 based on fluorescence and nanozyme activity of DNA templated silver nanocluster: enhanced sensitivity through G-quadruplex formation. Microchem J 205:111127","journal-title":"Microchem J"}],"container-title":["Microchimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-026-08287-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00604-026-08287-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00604-026-08287-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T08:17:02Z","timestamp":1786522622000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00604-026-08287-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,22]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["8287"],"URL":"https:\/\/doi.org\/10.1007\/s00604-026-08287-x","relation":{},"ISSN":["0026-3672","1436-5073"],"issn-type":[{"value":"0026-3672","type":"print"},{"value":"1436-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,22]]},"assertion":[{"value":"1 May 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This study was approved by the Ethics Committee of Zhejiang Provincial People's Hospital (approval No.: KY2024086). All procedures were performed in accordance with the Declaration of Helsinki. Human serum samples used in this study were obtained from healthy volunteers, and written informed consent was obtained from all participants prior to sample collection.","order":1,"name":"Ethics","label":"Ethical statement","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"562"}}