{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T18:59:08Z","timestamp":1789844348242,"version":"4.0.1"},"reference-count":5,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006447","name":"University of Zurich","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006447","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Neurochir"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Perfused placentas provide an excellent and accessible model for microvascular dissection, microsuturing and microanastomosis training \u2014 particularly in the early microsurgical learning curve. This way, a significant amount of live animals can be spared.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Method<\/jats:title>\n                <jats:p>We present the Zurich Microsurgery Lab protocol, detailing steps for obtaining, selecting, cleaning, flushing, cannulating, and preserving human placentas \u2014 as well as microsurgical training examples \u2014 in a tried-and-true, safe, cost-effective, and high-yield fashion.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Our technique enables highly realistic microsurgical training (microdissection, microvascular repair, microanastomosis) based on readily available materials. Proper handling, preparation, and preservation of the perfused placenta models is key.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00701-023-05847-5","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T04:23:56Z","timestamp":1700713436000},"page":"3821-3824","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["How we do it: the Zurich Microsurgery Lab technique for placenta preparation"],"prefix":"10.1007","volume":"165","author":[{"given":"Lara Maria","family":"H\u00f6bner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1039-2098","authenticated-orcid":false,"given":"Victor E.","family":"Staartjes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Colombo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Seb\u00f6k","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Regli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Esposito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,11,23]]},"reference":[{"key":"5847_CR1","doi-asserted-by":"publisher","first-page":"846","DOI":"10.3171\/2016.10.JNS162083","volume":"128","author":"MMR de Oliveira","year":"2018","unstructured":"de Oliveira MMR, Ferrarez CE, Ramos TM, Malheiros JA, Nicolato A, Machado CJ, Ferreira MT, de Oliveira FB, de Sousa CFPM, Costa PHV, Gusmao S, Lanzino G, Maestro RD (2018) Learning brain aneurysm microsurgical skills in a human placenta model: predictive validity. J Neurosurg 128:846\u2013852. https:\/\/doi.org\/10.3171\/2016.10.JNS162083","journal-title":"J Neurosurg"},{"key":"5847_CR2","doi-asserted-by":"publisher","first-page":"e854","DOI":"10.1016\/j.wneu.2020.11.034","volume":"146","author":"M Del Maestro","year":"2021","unstructured":"Del Maestro M, Rampini AD, Mauramati S, Giotta Lucifero A, Bertino G, Occhini A, Benazzo M, Galzio R, Luzzi S (2021) Dye-perfused human placenta for vascular microneurosurgery training: preparation protocol and validation testing. World Neurosurg 146:e854\u2013e864. https:\/\/doi.org\/10.1016\/j.wneu.2020.11.034","journal-title":"World Neurosurg"},{"key":"5847_CR3","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/S0007-1226(83)90066-8","volume":"36","author":"JC McGregor","year":"1983","unstructured":"McGregor JC, Wyllie FJ, Grigor KM (1983) Some anatomical observations on the human placenta as applied to microvascular surgical practice. Br J Plast Surg 36:387\u2013391. https:\/\/doi.org\/10.1016\/S0007-1226(83)90066-8","journal-title":"Br J Plast Surg"},{"key":"5847_CR4","doi-asserted-by":"publisher","first-page":"e694","DOI":"10.1016\/j.wneu.2018.07.246","volume":"119","author":"MM Oliveira","year":"2018","unstructured":"Oliveira MM, Wendling L, Malheiros JA, Nicolato A, Prosdocimi A, Guerra L, Costa PHV, Ferrarez CE, Ferreira MT, Sauvageau E, Hanel R (2018) Human placenta simulator for intracranial\u2013intracranial bypass: vascular anatomy and 5 bypass techniques. World Neurosurg 119:e694\u2013e702. https:\/\/doi.org\/10.1016\/j.wneu.2018.07.246","journal-title":"World Neurosurg"},{"key":"5847_CR5","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/0007-1226(85)90248-6","volume":"38","author":"N Waterhouse","year":"1985","unstructured":"Waterhouse N, Moss ALH, Townsend PLG (1985) The development of a dynamic model for microvascular research and practice using human placenta: a preliminary report. Br J Plast Surg 38:389\u2013393. https:\/\/doi.org\/10.1016\/0007-1226(85)90248-6","journal-title":"Br J Plast Surg"}],"container-title":["Acta Neurochirurgica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00701-023-05847-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00701-023-05847-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00701-023-05847-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T14:24:07Z","timestamp":1703168647000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00701-023-05847-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,23]]},"references-count":5,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["5847"],"URL":"https:\/\/doi.org\/10.1007\/s00701-023-05847-5","relation":{},"ISSN":["0942-0940"],"issn-type":[{"value":"0942-0940","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,23]]},"assertion":[{"value":"17 September 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 November 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This work is exempt from ethical review.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Giuseppe Esposito is lecturer for Aesculap B. Braun and Baxter AG. Luca Regli is lecturer for Aesculap B. Braun. The authors declare that the article and its content were composed in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}