{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T19:35:38Z","timestamp":1757619338401,"version":"3.44.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006451","name":"Hospices Civils de Lyon","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006451","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Neurochir"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Purpose<\/jats:title>\n            <jats:p>Accurate placement of electrodes within the subthalamic nucleus is critical for deep brain stimulation (STN-DBS) in Parkinson\u2019s disease (PD). Our objective was to compare microelectrode recording (MER) and an automatic MR-based segmentation tool (BrainLab Elements<jats:sup>TM<\/jats:sup>) for STN targeting and the determination of STN boundaries.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Seventy-eight PD patients were included. Electrode placement within the STN and STN entry and exit points were determined by both methods and compared for concordance.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Of 344 trajectories, 269 were inside the STN, with good concordance of both techniques (Fleiss\u2019 kappa 0.721, [95%CI 0.623, 0.819]). Concordance of MER and MR-based for the selection of the optimal trajectory was good (Fleiss\u2019 kappa 0.693, [95%CI 0.578, 0.808]), with less than 2.75mm difference between MER and MR-based for the STN entry (upper limit of agreement 2.752 [95%CI 2.365 to 3.138] mm; lower limit of agreement -2.406 [95%CI -2.793 to -2.020] mm) and exit points (upper limit of agreement 2.750 [95%CI 2.351 to 3.149] mm; lower limit of agreement -2.577mm [95%CI -2.976 to -2.178]).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>We demonstrated that MER and MR-based segmentation have a good concordance to determine STN boundaries during DBS surgery.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s00701-025-06619-z","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T05:57:35Z","timestamp":1753163855000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A comparison of electrophysiological microrecording versus automatic MR-based segmentation to determine subthalamic nucleus boundaries"],"prefix":"10.1007","volume":"167","author":[{"given":"Camilla","family":"de Laurentis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phane","family":"Thobois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teodor","family":"Danaila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chloe","family":"Laurencin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"Polo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phane","family":"Prange","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emile","family":"Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"6619_CR1","doi-asserted-by":"publisher","first-page":"S145","DOI":"10.1002\/mds.10156","volume":"17","author":"A Benazzouz","year":"2002","unstructured":"Benazzouz A, Breit S, Koudsie A, Pollak P, Krack P, Benabid A-L (2002) Intraoperative microrecordings of the subthalamic nucleus in Parkinson\u2019s disease. Mov Disord 17:S145\u2013S149. https:\/\/doi.org\/10.1002\/mds.10156","journal-title":"Mov Disord"},{"key":"6619_CR2","doi-asserted-by":"publisher","first-page":"e346","DOI":"10.1016\/j.wneu.2023.10.053","volume":"181","author":"HC Budnick","year":"2024","unstructured":"Budnick HC, Schneider D, Zauber SE, Witt TC, Gupta K (2024) Susceptibility-Weighted MRI Approximates Intraoperative Microelectrode Recording During Deep Brain Stimulation of the Subthalamic Nucleus for Parkinson\u2019s Disease. World Neurosurg 181:e346\u2013e355. https:\/\/doi.org\/10.1016\/j.wneu.2023.10.053","journal-title":"World Neurosurg"},{"key":"6619_CR3","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1016\/j.brainresbull.2010.01.001","volume":"81","author":"H-L Chan","year":"2010","unstructured":"Chan H-L, Lin M-A, Lee S-T, Tsai Y-T, Chao P-K, Wu T (2010) Complex analysis of neuronal spike trains of deep brain nuclei in patients with Parkinson\u2019s disease. Brain Res Bull 81:534\u2013542. https:\/\/doi.org\/10.1016\/j.brainresbull.2010.01.001","journal-title":"Brain Res Bull"},{"key":"6619_CR4","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1080\/02688697.2018.1478061","volume":"32","author":"C Chircop","year":"2018","unstructured":"Chircop C, Dingli N, Aquilina A, Zrinzo L, Aquilina J (2018) MRI-verified \u201casleep\u201d deep brain stimulation in Malta through cross border collaboration: clinical outcome of the first five years. Br J Neurosurg 32:365\u2013371. https:\/\/doi.org\/10.1080\/02688697.2018.1478061","journal-title":"Br J Neurosurg"},{"key":"6619_CR5","first-page":"47","volume-title":"International Review of Neurobiology","author":"VA Coenen","year":"2021","unstructured":"Coenen VA, Reisert M (2021) Chapter Three - DTI for brain targeting: Diffusion weighted imaging fiber tractography\u2014Assisted deep brain stimulation. In: Moro E, Polosan M, Hamani C (eds) International Review of Neurobiology. Academic Press, pp 47\u201367"},{"key":"6619_CR6","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1159\/000516098","volume":"99","author":"A De Roquemaurel","year":"2021","unstructured":"De Roquemaurel A, Wirth T, Vijiaratnam N, Ferreira F, Zrinzo L, Akram H, Foltynie T, Limousin P (2021) Stimulation Sweet Spot in Subthalamic Deep Brain Stimulation \u2013 Myth or Reality? A Critical Review of Literature. Stereotact Funct Neurosurg 99:425\u2013442. https:\/\/doi.org\/10.1159\/000516098","journal-title":"Stereotact Funct Neurosurg"},{"key":"6619_CR7","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1056\/NEJMoa060281","volume":"355","author":"G Deuschl","year":"2006","unstructured":"Deuschl G, Schade-Brittinger C, Krack P, Volkmann J, Sch\u00e4fer H, B\u00f6tzel K, Daniels C, Deutschl\u00e4nder A, Dillmann U, Eisner W, Gruber D, Hamel W, Herzog J, Hilker R, Klebe S, Klo\u00df M, Koy J, Krause M, Kupsch A et al (2006) A Randomized Trial of Deep-Brain Stimulation for Parkinson\u2019s Disease. N Engl J Med 355:896\u2013908. https:\/\/doi.org\/10.1056\/NEJMoa060281","journal-title":"N Engl J Med"},{"key":"6619_CR8","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1136\/jnnp.2010.205542","volume":"82","author":"T Foltynie","year":"2011","unstructured":"Foltynie T, Zrinzo L, Martinez-Torres I, Tripoliti E, Petersen E, Holl E, Aviles-Olmos I, Jahanshahi M, Hariz M, Limousin P (2011) MRI-guided STN DBS in Parkinson\u2019s disease without microelectrode recording: efficacy and safety. J Neurol Neurosurg Psychiatry 82:358\u2013363. https:\/\/doi.org\/10.1136\/jnnp.2010.205542","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"6619_CR9","doi-asserted-by":"publisher","first-page":"325","DOI":"10.3109\/10929080701730987","volume":"12","author":"T Guo","year":"2007","unstructured":"Guo T, Parrent AG, Peters TM (2007) Surgical targeting accuracy analysis of six methods for subthalamic nucleus deep brain stimulation. Comput Aided Surg 12:325\u2013334. https:\/\/doi.org\/10.3109\/10929080701730987","journal-title":"Comput Aided Surg"},{"key":"6619_CR10","doi-asserted-by":"publisher","first-page":"S162","DOI":"10.1002\/mds.10159","volume":"17","author":"MI Hariz","year":"2002","unstructured":"Hariz MI (2002) Complications of deep brain stimulation surgery. Mov Disord 17:S162\u2013S166. https:\/\/doi.org\/10.1002\/mds.10159","journal-title":"Mov Disord"},{"key":"6619_CR11","doi-asserted-by":"publisher","first-page":"3377","DOI":"10.1002\/hbm.23594","volume":"38","author":"A Horn","year":"2017","unstructured":"Horn A, Neumann W, Degen K, Schneider G, K\u00fchn AA (2017) Toward an electrophysiological \u201csweet spot\u201d for deep brain stimulation in the subthalamic nucleus. Hum Brain Mapp 38:3377\u20133390. https:\/\/doi.org\/10.1002\/hbm.23594","journal-title":"Hum Brain Mapp"},{"key":"6619_CR12","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.jdbs.2023.12.004","volume":"4","author":"C Isler","year":"2024","unstructured":"Isler C, Bas G (2024) New radiological techniques for planning of deep brain stimulation. Deep Brain Stimulation 4:24\u201328. https:\/\/doi.org\/10.1016\/j.jdbs.2023.12.004","journal-title":"Deep Brain Stimulation"},{"key":"6619_CR13","doi-asserted-by":"publisher","unstructured":"Kochanski R, Sani S (2018) Awake versus Asleep Deep Brain Stimulation Surgery: Technical Considerations and Critical Review of the Literature. Brain Sci 8. https:\/\/doi.org\/10.3390\/brainsci8010017","DOI":"10.3390\/brainsci8010017"},{"key":"6619_CR14","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.1056\/NEJMoa035275","volume":"349","author":"P Krack","year":"2003","unstructured":"Krack P, Batir A, Blercom NV, Chabard\u00e8s S, Fraix V, Ardouin C, Koudsie A, Dowsey Limousin P, Benazzouz A, LeBas JF, Benabid A-L, Pollak P (2003) Five-Year Follow-up of Bilateral Stimulation of the Subthalamic Nucleus in Advanced Parkinson\u2019s Disease. N Engl J Med 349:1925\u20131934. https:\/\/doi.org\/10.1056\/NEJMoa035275","journal-title":"N Engl J Med"},{"key":"6619_CR15","doi-asserted-by":"publisher","first-page":"100045","DOI":"10.1016\/j.gloepi.2020.100045","volume":"3","author":"MA Mansournia","year":"2021","unstructured":"Mansournia MA, Waters R, Nazemipour M, Bland M, Altman DG (2021) Bland-Altman methods for comparing methods of measurement and response to criticisms. Glob Epidemiol 3:100045. https:\/\/doi.org\/10.1016\/j.gloepi.2020.100045","journal-title":"Glob Epidemiol"},{"key":"6619_CR16","doi-asserted-by":"publisher","first-page":"276","DOI":"10.11613\/BM.2012.031","volume":"22","author":"ML McHugh","year":"2012","unstructured":"McHugh ML (2012) Interrater reliability: the kappa statistic. Biochem Med (Zagreb) 22:276\u2013282","journal-title":"Biochem Med (Zagreb)"},{"key":"6619_CR17","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/s40120-021-00233-8","volume":"10","author":"P van den Munckhof","year":"2021","unstructured":"van den Munckhof P, Bot M, Schuurman PR (2021) Targeting of the Subthalamic Nucleus in Patients with Parkinson\u2019s Disease Undergoing Deep Brain Stimulation Surgery. Neurol Ther 10:61\u201373. https:\/\/doi.org\/10.1007\/s40120-021-00233-8","journal-title":"Neurol Ther"},{"key":"6619_CR18","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1016\/j.brs.2019.05.001","volume":"12","author":"TAK Nguyen","year":"2019","unstructured":"Nguyen TAK, Nowacki A, Debove I, Petermann K, Tinkhauser G, Wiest R, Sch\u00fcpbach M, Krack P, Pollo C (2019) Directional stimulation of subthalamic nucleus sweet spot predicts clinical efficacy: Proof of concept. Brain Stimul 12:1127\u20131134. https:\/\/doi.org\/10.1016\/j.brs.2019.05.001","journal-title":"Brain Stimul"},{"key":"6619_CR19","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1093\/ons\/opx175","volume":"15","author":"A Nowacki","year":"2018","unstructured":"Nowacki A, Debove I, Fiechter M, Rossi F, Oertel MF, Wiest R, Sch\u00fcpbach M, Pollo C (2018) Targeting Accuracy of the Subthalamic Nucleus in Deep Brain Stimulation Surgery: Comparison Between 3 T T2-Weighted Magnetic Resonance Imaging and Microelectrode Recording Results. Oper Neurosurg 15:66. https:\/\/doi.org\/10.1093\/ons\/opx175","journal-title":"Oper Neurosurg"},{"key":"6619_CR20","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1159\/000507028","volume":"98","author":"WH Polanski","year":"2020","unstructured":"Polanski WH, Zolal A, Sitoci-Ficici KH, Hiepe P, Schackert G, Sobottka SB (2020) Comparison of Automatic Segmentation Algorithms for the Subthalamic Nucleus. Stereotact Funct Neurosurg 98:256\u2013262. https:\/\/doi.org\/10.1159\/000507028","journal-title":"Stereotact Funct Neurosurg"},{"key":"6619_CR21","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1093\/ons\/opz015","volume":"17","author":"PC Reinacher","year":"2019","unstructured":"Reinacher PC, V\u00e1rkuti B, Kr\u00fcger MT, Piroth T, Egger K, Roelz R, Coenen VA (2019) Automatic Segmentation of the Subthalamic Nucleus: A Viable Option to Support Planning and Visualization of Patient-Specific Targeting in Deep Brain Stimulation. Operative Surg 17:497\u2013502. https:\/\/doi.org\/10.1093\/ons\/opz015","journal-title":"Operative Surg"},{"key":"6619_CR22","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1007\/s00701-021-04790-7","volume":"163","author":"S Ribault","year":"2021","unstructured":"Ribault S, Simon E, Berthiller J, Polo G, Nunes A, Brinzeu A, Mertens P, Danaila T, Thobois S, Laurencin C (2021) Comparison of clinical outcomes and accuracy of electrode placement between robot-assisted and conventional deep brain stimulation of the subthalamic nucleus: a single-center study. Acta Neurochir 163:1327\u20131333. https:\/\/doi.org\/10.1007\/s00701-021-04790-7","journal-title":"Acta Neurochir"},{"key":"6619_CR23","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.1093\/brain\/awh571","volume":"128","author":"MC Rodriguez-Oroz","year":"2005","unstructured":"Rodriguez-Oroz MC, Obeso JA, Lang AE, Houeto J-L, Pollak P, Rehncrona S, Kulisevsky J, Albanese A, Volkmann J, Hariz MI, Quinn NP, Speelman JD, Guridi J, Zamarbide I, Gironell A, Molet J, Pascual-Sedano B, Pidoux B, Bonnet AM et al (2005) Bilateral deep brain stimulation in Parkinson\u2019s disease: a multicentre study with 4 years follow-up. Brain 128:2240\u20132249. https:\/\/doi.org\/10.1093\/brain\/awh571","journal-title":"Brain"},{"key":"6619_CR24","doi-asserted-by":"publisher","first-page":"2307","DOI":"10.1007\/s00701-011-1081-7","volume":"153","author":"JR Schlaier","year":"2011","unstructured":"Schlaier JR, Habermeyer C, Warnat J, Lange M, Janzen A, Hochreiter A, Proescholdt M, Brawanski A, Fellner C (2011) Discrepancies between the MRI- and the electrophysiologically defined subthalamic nucleus. Acta Neurochir 153:2307\u20132318. https:\/\/doi.org\/10.1007\/s00701-011-1081-7","journal-title":"Acta Neurochir"},{"key":"6619_CR25","doi-asserted-by":"publisher","unstructured":"Tonge M, Kocabicak E, Ackermans L, Kuijf M, Temel Y (2015) Final electrode position in subthalamic nucleus deep brain stimulation surgery: a comparison of indirect and direct targeting methods. Turk Neurosurg. https:\/\/doi.org\/10.5137\/1019-5149.JTN.13739-14.1","DOI":"10.5137\/1019-5149.JTN.13739-14.1"},{"key":"6619_CR26","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1111\/jon.13133","volume":"33","author":"T Tonroe","year":"2023","unstructured":"Tonroe T, McDermott H, Pearce P, Acevedo N, Thevathasan W, Xu SS, Bulluss K, Perera T (2023) Anatomical targeting for electrode localization in subthalamic nucleus deep brain stimulation: A comparative study. J Neuroimaging 33:792\u2013801. https:\/\/doi.org\/10.1111\/jon.13133","journal-title":"J Neuroimaging"},{"key":"6619_CR27","doi-asserted-by":"publisher","first-page":"e297","DOI":"10.1371\/journal.pmed.0040297","volume":"4","author":"JP Vandenbroucke","year":"2007","unstructured":"Vandenbroucke JP, von Elm E, Altman DG, G\u00f8tzsche PC, Mulrow CD, Pocock SJ, Poole C, Schlesselman JJ, Egger M, STROBE Initiative (2007) Strengthening the Reporting of Observational Studies in Epidemiology (STROBE): Explanation and Elaboration. PLoS Med 4:e297. https:\/\/doi.org\/10.1371\/journal.pmed.0040297","journal-title":"PLoS Med"},{"key":"6619_CR28","doi-asserted-by":"publisher","first-page":"105372","DOI":"10.1016\/j.nbd.2021.105372","volume":"155","author":"Z Yin","year":"2021","unstructured":"Yin Z, Zhu G, Zhao B, Bai Y, Jiang Y, Neumann W-J, K\u00fchn AA, Zhang J (2021) Local field potentials in Parkinson\u2019s disease: A frequency-based review. Neurobiol Dis 155:105372. https:\/\/doi.org\/10.1016\/j.nbd.2021.105372","journal-title":"Neurobiol Dis"},{"key":"6619_CR29","doi-asserted-by":"publisher","unstructured":"Zeiler F, Wilkinson M, Krcek J (2013) Subthalamic nucleus deep brain stimulation: an invaluable role for MER. Can J Neurol Sci 40. https:\/\/doi.org\/10.1017\/s0317167100014682","DOI":"10.1017\/s0317167100014682"}],"container-title":["Acta Neurochirurgica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00701-025-06619-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00701-025-06619-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00701-025-06619-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T17:20:10Z","timestamp":1757265610000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00701-025-06619-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,22]]},"references-count":29,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["6619"],"URL":"https:\/\/doi.org\/10.1007\/s00701-025-06619-z","relation":{},"ISSN":["0942-0940"],"issn-type":[{"type":"electronic","value":"0942-0940"}],"subject":[],"published":{"date-parts":[[2025,7,22]]},"assertion":[{"value":"13 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study protocol was reviewed and approved by the local ethics committee of the Hospices Civils de Lyon (Comit\u00e9 Scientifique et \u00c9thique des Hospices Civils de Lyon), IRB (Internal Review Board) 00013204, AGORA number 23-5439.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Study approval statement"}},{"value":"Informed consent was obtained from all the participants\/legal guardian of participants prior to the study, to use their data in a completely anonymized form. Written informed consent was not deemed necessary because the study was conducted using regularly collected data. This study protocol and consent procedure was reviewed and approved by the local ethics committee of the Hospices Civils de Lyon (Comit\u00e9 Scientifique et \u00c9thique des Hospices Civils de Lyon, Lyon, France), approval numbers IRB (Internal Review Board) 00013204, AGORA number 23-5439 (conformit\u00e9 MR004 n\u00b023-5439), decision date 20\/05\/2024, and non-opposition was collected from patients. The study was in line with local and national guidelines.Our study has therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Human ethics and consent to participate declaration"}},{"value":"C. de Laurentis declares that she has no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.S. Thobois declares honoraria from Boston, Medtronic, Abbvie, Merz, NHC, MDS for consulting, board, or conferences; meeting and travel grant from the MDS and Abbvie.T. Danaila declares honoraria from Abbvie and Orkyn.C. Laurencin declares travel grants from Abbvie, and honoraria from Orphalan.G. Polo declares that he has no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.S. Prange declares honoraria from Abbvie for consulting.E. Simon declares honoraria from Boston & BrainLab for conferences, meeting and travel grant from Boston.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"199"}}